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<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 μL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
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<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
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<p><small><strong> Figure 1. FLAG antibody western blot results </strong><br />Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 μL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.</small></p>
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<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
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<p><small><strong> Figure 2. FLAG antibody western blot results </strong><br />Monoclonal Antibody to detect FLAGTM conjugated proteins detects both <g class="gr_ gr_14 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="14" data-gr-id="14">C terminal</g> linked and <g class="gr_ gr_15 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="15" data-gr-id="15">N terminal</g> linked FLAGTM tagged recombinant proteins by western blot.</small></p>
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ReflectionMethod::invokeArgs() - [internal], line ??
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×
