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<p><small><strong> Figure 1. FUBP1 ChIP results</strong><br />ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.</small></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br />FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 3. IHC</strong><br />Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.</small></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><small><strong> Figure 1. FUBP1 ChIP results</strong><br />ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.</small></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br />FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 3. IHC</strong><br />Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.</small></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br />FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 3. IHC</strong><br />Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.</small></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /> FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining. </small></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br />FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410233-IP.jpg" alt="FUBP1 Antibody validated in Immunoprecipitates" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br />FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410233-IHC.jpg" alt="Antibody validated in Immunohistochemical" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 3. IHC</strong><br />Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410233-WB.jpg" alt="Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410233-IF.png" alt="Antibody validated in Immunofluorescent" caption="false" width="284" height="114" /></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br />FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 3. IHC</strong><br />Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.</small></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<td>1:100 - 1:1,000</td>
<td>Fig 4</td>
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<p><small><strong> Figure 1. FUBP1 ChIP results</strong><br />ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE.</small></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br />FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 3. IHC</strong><br />Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution.</small></p>
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<p><small><strong> Figure 4. Western blot</strong><br />Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% <g class="gr_ gr_27 gr-alert gr_spell gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="27" data-gr-id="27">SDS PAGE</g> FUBP1 antibody diluted 1:5,000</small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /><g class="gr_ gr_29 gr-alert gr_gramm gr_disable_anim_appear Grammar multiReplace" id="29" data-gr-id="29">FUBP1</g> antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. FUBP1 ChIP results</strong><br /> ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or FUBP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 FUSE. </small></p>
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<p><small><strong> Figure 2. FUBP1 IP results</strong><br /> FUBP1 antibody immunoprecipitates FUBP1 protein in IP experiments. IP Sample: 293T whole cell extract A: 30 μg whole cell extract of FUBP1 protein expressing 293T cells B: Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FUBP1 by 2.5 μg of FUBP1 antibody. The immunoprecipitated FUBP1 protein was detected by western blot with the FUBP1 antibody diluted 1:1,000. </small></p>
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<p><small><strong> Figure 3. IHC</strong><br /> Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using FUBP1 antibody at a 1:500 dilution. </small></p>
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<p><small><strong> Figure 4. Western blot</strong><br /> Sample: 30 μg of whole cell lysate A: Jurkat B: K562 C: THP-1 D: NCI-H929 7.5% SDS PAGE FUBP1 antibody diluted 1:5,000 </small></p>
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<p><small><strong> Figure 5. FUBP1 IF results</strong><br /> FUBP1 antibody detects FUBP1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FUBP1 protein stained by FUBP1 antibody diluted 1:500. Blue: Hoechst 33342 staining. </small></p>
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$application = array(
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'slug' => 'chip-qpcr-antibodies',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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