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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-ChIP.png" alt="H3T6pK9me3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-IF.png" alt="H3T6pK9me3 Antibody validated in Immunofluorescence " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2 H3T6pK9me3 antibody Immunofluorescence results</strong><br /> Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-WB.png" alt="H3T6pK9me3 Antibody validated in Western blot " style="display: block; margin-left: auto; margin-right: auto;" width="75" height="188" /></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-WB-2.png" alt="H3T6pK9me3 Antibody validated in Western blot " style="display: block; margin-left: auto; margin-right: auto;" width="197" height="185" /></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-Dotblot.png" alt="H3T6pK9me3 Antibody validated in Dot blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><small><strong> Figure 2 H3T6pK9me3 antibody Immunofluorescence results</strong><br /> Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue). </small></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
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<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><small><strong> Figure 2 H3T6pK9me3 antibody Immunofluorescence results</strong><br /> Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue). </small></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-WB-2.png" alt="H3T6pK9me3 Antibody validated in Western blot " style="display: block; margin-left: auto; margin-right: auto;" width="197" height="185" /></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-ChIP.png" alt="H3T6pK9me3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-IF.png" alt="H3T6pK9me3 Antibody validated in Immunofluorescence " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2 H3T6pK9me3 antibody Immunofluorescence results</strong><br /> Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-WB.png" alt="H3T6pK9me3 Antibody validated in Western blot " style="display: block; margin-left: auto; margin-right: auto;" width="75" height="188" /></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-WB-2.png" alt="H3T6pK9me3 Antibody validated in Western blot " style="display: block; margin-left: auto; margin-right: auto;" width="197" height="185" /></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-Dotblot.png" alt="H3T6pK9me3 Antibody validated in Dot blot " style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
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<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
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<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
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<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
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<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-ChIP.png" alt="H3T6pK9me3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><small><strong> Figure 2 H3T6pK9me3 antibody Immunofluorescence results</strong><br /> Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue). </small></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-WB-2.png" alt="H3T6pK9me3 Antibody validated in Western blot " style="display: block; margin-left: auto; margin-right: auto;" width="197" height="185" /></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410285-ChIP.png" alt="H3T6pK9me3 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. H3T6pK9me3 antibody ChIP results</strong><br /> Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin. </small></p>
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<p><small><strong> Figure 2 H3T6pK9me3 antibody Immunofluorescence results</strong><br /> Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue). </small></p>
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<p><small><strong> Figure 3. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 4. H3T6pK9me3 antibody Western blot results</strong><br /> Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p>
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<p><small><strong> Figure 5. H3T6pK9me3 antibody Dot blot results</strong><br /> Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
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<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '4207',
'product_id' => '2404',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'eng'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '345',
'name' => 'Datasheet H3T6p C15410282',
'description' => 'Datasheet description',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/Datasheet_H3T6p_C15410282.pdf',
'slug' => 'datasheet-h3t6p-c15410282',
'meta_keywords' => null,
'meta_description' => null,
'modified' => '2015-07-07 11:47:44',
'created' => '2015-07-07 11:47:44',
'ProductsDocument' => array(
'id' => '498',
'product_id' => '2404',
'document_id' => '345'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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