H3T6pK9me3 polyclonal antibody

Figure 1. H3T6pK9me3 antibody ChIP results
Chromatin Immunoprecipitation of H3T6pK9me3 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me3 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, and normalized to the input chromatin.

Figure 2 H3T6pK9me3 antibody Immunofluorescence results
Immunofluorescence of H3T6pK9me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody incubated at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me3 is nuclear and chromosomal. Staining: H3T6pK9me3 is expressed in green and the nuclei are counterstained with DAPI (blue).

Figure 3. H3T6pK9me3 antibody Western blot results
Western Blot of H3T6pK9me3 antibody. 30 μg C. elegans embryo lysate. Primary antibody: diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

Figure 4. H3T6pK9me3 antibody Western blot results
Western Blot of H3T6pK9me3 antibody. 30 μg HeLa histone extracts. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

Figure 5. H3T6pK9me3 antibody Dot blot results
Dot Blot of H3T6pK9me3 antibody. Lane 1: T6. Lane 2: T6p. Lane 3: T6pK9me1. Lane 4: T6pK9me2. Lane 5: T6pK9me3. Lane 6: K9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lane 10: K9ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.