Diagenode

H4K8ac Antibody

Catalog Number
Format
Price
C15310103
(CS-103-100)
100 µl
$380.00
  Bulk order



Polyclonal antibody raised in rabbit against histone H4 containing the acetylated lysine 8 (H4K8ac), using a KLH-conjugated synthetic peptide.

LotA156-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μl/ChIP Fig 1
ELISA 1:500 Fig 2
Dot Blotting 1:20,000 Fig 3
Western Blotting 1:250 Fig 4

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data

    H4K8ac Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode crude serum directed against H4K8ac
    Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H4K8ac (cat. No. CS-103-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right. Lane 2 shows the result of the Western analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide (cat. No. sp-103-050) for 1 hour at room temperature.

         H4K8ac Antibody ELISA validation

    Figure 2. Determination of the antibody titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human H4K8ac (cat. No. CS-103-100). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:17,500.

    H4K8ac Antibody validated in Dot Blot

    Figure 3. Cross reactivity test using the Diagenode antibody directed against H4K8ac
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H4K8ac (cat. No. CS-103-100) with peptides containing other modifications of histone H4 and H3 and an unmodified histone H4 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the modification of interest.

    H4K8ac Antibody validated in Western Blot

    Figure 4. Western blot analysis using the Diagenode crude serum against H4K8ac
    Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2c (Cat. No. CS-105-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H4 is associated with active gene transcription.

  •  Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet H4K8ac CS-103-100 DATASHEET
    Datasheet description
    Download
  •  Safety sheets
    H4K8ac Antibody SDS US en Download
    H4K8ac Antibody SDS GB en Download
    H4K8ac Antibody SDS BE fr Download
    H4K8ac Antibody SDS FR fr Download
    H4K8ac Antibody SDS ES es Download
    H4K8ac Antibody SDS DE de Download
    H4K8ac Antibody SDS JP ja Download
    H4K8ac Antibody SDS BE nl Download
  •  Publications

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