His tag Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15200191
(MAb-191-050)

50 μg

$380.00
Bulk order

Monoclonal antibody raised in mouse against the 6xHis tag (amino acid sequence HHHHHH) using a KLH-conjugated synthetic peptide. The antibody recognizes the Histidine epitope which is one of the most popular protein tags. The Diagenode His tag antibody is extremely specific and allows unambiguous identification and quantitative analysis of His-tagged proteins.

Lot	001
Concentration	2.1 µg/µl
Species reactivity	The His monoclonal is a multi-purpose antibody that recognizes His epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
Type	Monoclonal
Purity	Protein A purified monoclonal antibody in PBS (pH 7.2).
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution^*	References
Western Blotting	1:1,000 - 1:3,000	Fig 1, 2
IP	1:150 - 1:200
IS	1:500 - 1:1,000

^* Please note that the optimal antibody amount should be determined by the end-user for each specific assay condition.

Validation Data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag
Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody.

Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag
Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody.

Documents
Datasheet HisTag MAb-191-050 DATASHEET
Datasheet description Download

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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<td>Western Blotting</td>
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<td>Fig 1, 2</td>
</tr>
<tr>
<td>IP</td>
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			'id' => '761',
			'name' => 'His tag antibody SDS BE fr',
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			'countries' => 'BE',
			'modified' => '2020-09-21 12:08:22',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200191_WB-1.png" alt="Western Blot" width="110" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200191_WB-2.png" alt="Western Blot" width="150" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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<td>Fig 1, 2</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount should be determined by the end-user for each specific assay condition.</small></p>',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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		'concentration' => '2.1 &micro;g/&micro;l',
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<th>References</th>
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<td>Western Blotting</td>
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<td>Fig 1, 2</td>
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<td>IP</td>
<td>1:150 - 1:200</td>
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<td>IS</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount should be determined by the end-user for each specific assay condition.</small></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'description' => '<p><span>Monoclonal antibody raised in mouse against the 6xHis tag (amino acid sequence HHHHHH) using a KLH-conjugated synthetic peptide. The antibody recognizes the Histidine epitope which is one of the most popular protein tags. The Diagenode His tag antibody is extremely specific and allows unambiguous identification and quantitative analysis of His-tagged proteins.</span></p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200191_WB-1.png" alt="Western Blot" width="110" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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<thead>
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<th>Suggested dilution<sup>*</sup></th>
<th>References</th>
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<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:3,000</td>
<td>Fig 1, 2</td>
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<tr>
<td>IP</td>
<td>1:150 - 1:200</td>
<td></td>
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<tr>
<td>IS</td>
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<td></td>
</tr>
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<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount should be determined by the end-user for each specific assay condition.</small></p>',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '291',
	'name' => 'Datasheet HisTag MAb-191-050',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_HisTag_MAb-191-050.pdf',
	'slug' => 'datasheet-histag-mab-191-050',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:43',
	'created' => '2015-07-07 11:47:43',
	'ProductsDocument' => array(
		'id' => '574',
		'product_id' => '2006',
		'document_id' => '291'
	)
)
$sds = array(
	'id' => '764',
	'name' => 'His tag antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/His/SDS-C15200191-His_tag_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2020-09-21 12:09:46',
	'created' => '2020-09-21 12:09:46',
	'ProductsSafetySheet' => array(
		'id' => '1395',
		'product_id' => '2006',
		'safety_sheet_id' => '764'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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His tag antibody SDS GB en	Download
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