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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the His protein tag </strong><br />Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Diagenode monoclonal antibody directed against His (Cat. No. MAb-191-050). The antibody was used at a dilution of 1:1,000. Figure 2 shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. </small></p>
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include - APP/View/Products/view.ctp, line 755
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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include - APP/View/Products/view.ctp, line 755
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View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies',
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[maximum depth reached]
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'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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'all_format' => false,
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'slug' => 'all-antibodies',
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'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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'created' => '2015-11-02 14:49:22',
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'type' => 'Datasheet',
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'modified' => '2015-07-07 11:47:43',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
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)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
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'name' => 'product/antibodies/ab-chip-icon.png',
'alt' => 'Antibody ChIP icon',
'modified' => '2020-08-12 11:52:55',
'created' => '2018-03-15 15:52:35',
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'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
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'modified' => '2020-09-21 12:11:33',
'created' => '2020-09-21 12:11:33',
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'modified' => '2020-09-21 12:09:05',
'created' => '2020-09-21 12:09:05',
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(int) 3 => array(
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'name' => 'His tag antibody SDS JP ja',
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'modified' => '2020-09-21 12:11:06',
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'ProductsSafetySheet' => array(
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(int) 4 => array(
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(int) 4 => 'DK',
(int) 5 => 'NO',
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(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
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$chipseq_service = array(
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(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
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$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
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'position' => '10',
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'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
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'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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