Alternative names: JHDM3A, KDM4A, JMJD2
Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.
| Lot | A288-0011 |
|---|---|
| Concentration | 0.66 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:500 | Fig 1 |
| Western blotting | 1:500 | Fig 2 |
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2a (Cat. No. pAb-126-050), crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2a
Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. pAb-126-050) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.
 How to properly cite our product/service in your workWe strongly recommend using this: JMJD2a Antibody (Hologic Diagenode Cat# C15410126 Lot# A288-0011). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
