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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2a (Cat. No. pAb-126-050), crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br />Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. pAb-126-050) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2a (Cat. No. pAb-126-050), crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br />Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. pAb-126-050) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span>Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2a (Cat. No. pAb-126-050), crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br />Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. pAb-126-050) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.</small></p>
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JMJD2a (UniProtKB/Swiss-Prot entry O75164), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.',
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2a (Cat. No. pAb-126-050), crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br />Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. pAb-126-050) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.</small></p>
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'safety_sheet_id' => '3116'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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