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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<td>Fig 1, 2</td>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<td>Fig 1, 2</td>
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<td>Fig 3</td>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-if.png" alt="Immunofluorescence" /></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-if.png" alt="Immunofluorescence" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<td>Western blotting</td>
<td>1:5,000</td>
<td>Fig 1, 2</td>
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<td>IF</td>
<td>2 µl/IP</td>
<td>Fig 3</td>
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<td>IP</td>
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<td>Fig 4</td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-wb-1.png" alt="Western blot" /></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-if.png" alt="Immunofluorescence" /></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<div class="small-4 columns">
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<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<th>References</th>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p>
<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
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<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
<div class="small-9 medium-9 large-9 columns">
<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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