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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-wb-1.png" alt="Western blot" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-wb-2.png" alt="Western blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-ip.png" alt="Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-if.png" alt="Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.</p>',
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'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
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'lot' => 'A2577-001',
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'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Polyclonal',
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'application_table' => '<table>
<thead>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tbody>
<tr>
<td>Western blotting</td>
<td>1:5,000</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>IF</td>
<td>2 µl/IP</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td>1:500</td>
<td>Fig 4</td>
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</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
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'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-wb-1.png" alt="Western blot" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-wb-2.png" alt="Western blot" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-ip.png" alt="Immunoprecipitation" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-if.png" alt="Immunofluorescence" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p>
<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
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<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
<div class="small-9 medium-9 large-9 columns">
<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-wb-1.png" alt="Western blot" /></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against LbCRISPR/Cpf1 (Cat. No. C15310263), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-wb-2.png" alt="Western blot" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (<span>Cat</span>. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-ip.png" alt="Immunoprecipitation" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 3. IP using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />IP was performed on whole cell extracts (350 µg) from HEK293 cells transfected with HA-tagged LbCpf1 using 2 µl of the Diagenode antibody against LbCRISPR/Cpf1 (<span>Cat</span>. No. C15310263). The immunoprecipitated proteins were subsequently analysed by Western blot with an anti-HA antibody. Lane 2 and 3 show the result of the IP with the LbCRISPR/Cpf1 andtibody and with beads only, respectively. The input (17.5 µg) is shown in lane 1.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310263-if.png" alt="Immunofluorescence" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 4. Immunofluorescence using the Diagenode antibody directed against LbCRISPR/Cpf1</strong><br />Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (<span>Cat</span>. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
</div>
</div>',
'label2' => 'Background',
'info2' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.</p>',
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'format' => '100 µl',
'catalog_number' => 'C15310263-100',
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'sf_code' => 'C15310263-D001-001161',
'type' => 'FRE',
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'meta_description' => 'L. bacterium CRISPR/Cpf1 Polyclonal Antibody validated in IF, IP and WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-01-16 12:59:12',
'created' => '2016-09-12 12:36:04'
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