Request a quote for a bulk order for NFYA Antibody. Please fill out the form here below. Your local sales account manager will get in touch with you shortly and send you a quotation based on your requirements.
Alternative names: NF-YA, HAP2, CBF-A, CBF-B
Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.
| Lot | 001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal ChIP grade, ChIP-seq grade |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 1:1,000 | Fig 1, 2 |
| Western Blotting | 1:1,000 | Fig 3 |
| Immunohistochemistry | 1:400 | Fig 4 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA
ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA
ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.
Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA
Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.
Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA
Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).
NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IHC Immunohistochemistry Read more |
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| NFYA polyclonal antibody C15310261 DATASHEET Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), us... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| SDS C15310261 NFYA Antibody US en | Download |
| SDS C15310261 NFYA Antibody GB en | Download |
| SDS C15310261 NFYA Antibody ES es | Download |
| SDS C15310261 NFYA Antibody FR fr | Download |
| SDS C15310261 NFYA Antibody BE fr | Download |
| SDS C15310261 NFYA Antibody BE nl | Download |
| SDS C15310261 NFYA Antibody DE de | Download |
| SDS C15310261 NFYA Antibody JP ja | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: NFYA Antibody (Diagenode Cat# C15310261 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody' $meta_description = 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B' $meta_title = 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode' $product = array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '906', 'name' => 'NFYA polyclonal antibody C15310261', 'description' => '<p>Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYA_polyclonal_antibody_C15310261.pdf', 'slug' => 'datasheet_nfya_polyclonal_antibody_c15310261', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-22 13:48:02', 'created' => '2016-06-22 13:48:02', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3415', 'name' => 'SDS C15310261 NFYA Antibody US en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-16 12:39:04', 'created' => '2024-01-16 12:39:04', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3409', 'name' => 'SDS C15310261 NFYA Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-16 12:37:10', 'created' => '2024-01-16 12:37:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3411', 'name' => 'SDS C15310261 NFYA Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-16 12:37:49', 'created' => '2024-01-16 12:37:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3410', 'name' => 'SDS C15310261 NFYA Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-16 12:37:30', 'created' => '2024-01-16 12:37:30', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3414', 'name' => 'SDS C15310261 NFYA Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:50', 'created' => '2024-01-16 12:38:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3413', 'name' => 'SDS C15310261 NFYA Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:29', 'created' => '2024-01-16 12:38:29', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3412', 'name' => 'SDS C15310261 NFYA Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-16 12:38:08', 'created' => '2024-01-16 12:38:08', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4602', 'product_id' => '2818', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( 'id' => '2128', 'product_id' => '2818', 'document_id' => '11' ) ) $sds = array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( 'id' => '5530', 'product_id' => '2818', 'safety_sheet_id' => '3416' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody' $meta_description = 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B' $meta_title = 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode' $product = array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '906', 'name' => 'NFYA polyclonal antibody C15310261', 'description' => '<p>Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYA_polyclonal_antibody_C15310261.pdf', 'slug' => 'datasheet_nfya_polyclonal_antibody_c15310261', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-22 13:48:02', 'created' => '2016-06-22 13:48:02', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3415', 'name' => 'SDS C15310261 NFYA Antibody US en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-16 12:39:04', 'created' => '2024-01-16 12:39:04', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3409', 'name' => 'SDS C15310261 NFYA Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-16 12:37:10', 'created' => '2024-01-16 12:37:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3411', 'name' => 'SDS C15310261 NFYA Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-16 12:37:49', 'created' => '2024-01-16 12:37:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3410', 'name' => 'SDS C15310261 NFYA Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-16 12:37:30', 'created' => '2024-01-16 12:37:30', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3414', 'name' => 'SDS C15310261 NFYA Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:50', 'created' => '2024-01-16 12:38:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3413', 'name' => 'SDS C15310261 NFYA Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:29', 'created' => '2024-01-16 12:38:29', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3412', 'name' => 'SDS C15310261 NFYA Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-16 12:38:08', 'created' => '2024-01-16 12:38:08', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4602', 'product_id' => '2818', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( 'id' => '2128', 'product_id' => '2818', 'document_id' => '11' ) ) $sds = array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( 'id' => '5530', 'product_id' => '2818', 'safety_sheet_id' => '3416' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody' $meta_description = 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B' $meta_title = 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode' $product = array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '906', 'name' => 'NFYA polyclonal antibody C15310261', 'description' => '<p>Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYA_polyclonal_antibody_C15310261.pdf', 'slug' => 'datasheet_nfya_polyclonal_antibody_c15310261', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-22 13:48:02', 'created' => '2016-06-22 13:48:02', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3415', 'name' => 'SDS C15310261 NFYA Antibody US en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-16 12:39:04', 'created' => '2024-01-16 12:39:04', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3409', 'name' => 'SDS C15310261 NFYA Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-16 12:37:10', 'created' => '2024-01-16 12:37:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3411', 'name' => 'SDS C15310261 NFYA Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-16 12:37:49', 'created' => '2024-01-16 12:37:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3410', 'name' => 'SDS C15310261 NFYA Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-16 12:37:30', 'created' => '2024-01-16 12:37:30', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3414', 'name' => 'SDS C15310261 NFYA Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:50', 'created' => '2024-01-16 12:38:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3413', 'name' => 'SDS C15310261 NFYA Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:29', 'created' => '2024-01-16 12:38:29', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3412', 'name' => 'SDS C15310261 NFYA Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-16 12:38:08', 'created' => '2024-01-16 12:38:08', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4602', 'product_id' => '2818', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( 'id' => '2128', 'product_id' => '2818', 'document_id' => '11' ) ) $sds = array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( 'id' => '5530', 'product_id' => '2818', 'safety_sheet_id' => '3416' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody' $meta_description = 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B' $meta_title = 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode' $product = array( 'Product' => array( 'id' => '2818', 'antibody_id' => '481', 'name' => 'NFYA Antibody', 'description' => '<p>Alternative names: <strong>NF-YA</strong>, <strong>HAP2</strong>, <strong>CBF-A</strong>, <strong>CBF-B</strong></p> <p>Polyclonal antibody raised in rabbit against <strong>NFYA (Nuclear transcription factor-Y, subunit A)</strong>, using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-chip.jpg" alt="NFYA Antibody ChIP Grade" caption="false" width="288" height="220" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p> </div> <div class="small-10 columns"> <p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15310261', 'old_catalog_number' => '', 'sf_code' => 'C15310261-D001-001161', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 16, 2017', 'slug' => 'nfya-polyclonal-antibody-100-ul', 'meta_title' => 'NFYA Antibody - ChIP-seq Grade (C15310261) | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'NFYA (Nuclear transcription factor-Y, subunit A) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB and IHC. Alternative names: NF-YA, HAP2, CBF-A, CBF-B', 'modified' => '2024-01-16 12:42:43', 'created' => '2016-06-22 13:46:12', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '481', 'name' => 'NFYA polyclonal antibody', 'description' => 'NFYA (UniProt/Swiss-Prot entry P23511) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. Depending on the cofactors involved, NFY can function both as an activator or a repressor, The NFYA subunit is responsible for the sequence specific interactions of the complex, suggesting a role as regulatory subunit. NFYA regulates the transcription of the core clock component ARNTL/BMAL1.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Whole antiserum', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq *</td> <td>1:1,000</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:400</td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:04:16', 'created' => '2016-06-22 13:38:17', 'select_label' => '481 - NFYA polyclonal antibody (001 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '906', 'name' => 'NFYA polyclonal antibody C15310261', 'description' => '<p>Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYA_polyclonal_antibody_C15310261.pdf', 'slug' => 'datasheet_nfya_polyclonal_antibody_c15310261', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-22 13:48:02', 'created' => '2016-06-22 13:48:02', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '3415', 'name' => 'SDS C15310261 NFYA Antibody US en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2024-01-16 12:39:04', 'created' => '2024-01-16 12:39:04', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3409', 'name' => 'SDS C15310261 NFYA Antibody GB en', 'language' => 'en', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2024-01-16 12:37:10', 'created' => '2024-01-16 12:37:10', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3411', 'name' => 'SDS C15310261 NFYA Antibody ES es', 'language' => 'es', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2024-01-16 12:37:49', 'created' => '2024-01-16 12:37:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3410', 'name' => 'SDS C15310261 NFYA Antibody FR fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2024-01-16 12:37:30', 'created' => '2024-01-16 12:37:30', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3414', 'name' => 'SDS C15310261 NFYA Antibody BE fr', 'language' => 'fr', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:50', 'created' => '2024-01-16 12:38:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3413', 'name' => 'SDS C15310261 NFYA Antibody BE nl', 'language' => 'nl', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2024-01-16 12:38:29', 'created' => '2024-01-16 12:38:29', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3412', 'name' => 'SDS C15310261 NFYA Antibody DE de', 'language' => 'de', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2024-01-16 12:38:08', 'created' => '2024-01-16 12:38:08', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( 'id' => '4602', 'product_id' => '2818', 'application_id' => '43' ) ) $slugs = array( (int) 0 => 'chip-qpcr-antibodies' ) $applications = array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'locale' => 'eng' ) $description = '' $name = 'ChIP-qPCR (ab)' $document = array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( 'id' => '2128', 'product_id' => '2818', 'document_id' => '11' ) ) $sds = array( 'id' => '3416', 'name' => 'SDS C15310261 NFYA Antibody JP ja', 'language' => 'ja', 'url' => 'files/SDS/NFYA/SDS-C15310261-NFYA_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2024-01-16 12:39:25', 'created' => '2024-01-16 12:39:25', 'ProductsSafetySheet' => array( 'id' => '5530', 'product_id' => '2818', 'safety_sheet_id' => '3416' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.
