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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p>
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<p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p>
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<td>1:1,000</td>
<td>Fig 1, 2</td>
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<p><small>* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>',
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<p>Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p>
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<p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p>
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<p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p>
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<p>Polyclonal antibody raised in rabbit against NFYA (Nuclear transcription factor-Y, subunit A), using a KLH conjugated synthetic peptide from the N-terminus of the protein.</p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-a.jpg" alt="NFYA Antibody ChIP-seq Grade" caption="false" width="922" height="110" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-b.jpg" alt="NFYA Antibody for ChIP-seq " caption="false" width="922" height="259" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-c.jpg" alt="NFYA Antibody for ChIP-seq assay" caption="false" width="922" height="191" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/C15310261-chipseq-d.jpg" alt="NFYA Antibody validated in ChIP-seq " caption="false" width="922" height="194" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p>
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<p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p>
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<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-Wb.jpg" alt="NFYA Antibody validated in western Blot" caption="false" width="129" height="161" /></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310261-IF.jpg" alt="NFYA Antibody validated in Immunohistochemistry " caption="false" width="129" height="127" /></p>
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<p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYA (Cat. No. C15310261) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYA</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells using 1 μl of the Diagenode antibody against NFYA (Cat. No. C15310261) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NFYA</strong><br />Nuclear extracts from CHO-7 cells (35 μg) were analysed by Western blot using the Diagenode antibody against NFYA (Cat. No. C15310261) diluted 1:1,000. Figure 3 shows a doublet representing both isoforms of NFYA.</small></p>
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<p><small><strong>Figure 4. Immunohistochemistry using the Diagenode antibody directed against NFYA</strong><br />Formalin fixed paraffin embedded human prostate tissue was stained with the Diagenode antibody against NFYA (cat. No. C15310261) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of NFYA (red).</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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[main] - APP/webroot/index.php, line 118
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