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Polyclonal antibody raised in rabbit against NFYB (nuclear transcription factor Y, beta), using a recombinant protein.
| Lot | 42817 |
|---|---|
| Concentration | 0.42 μg/μl |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1% BSA, 20% glycerol and 0.025% proclin300. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 2 μg/IP | Fig 1, 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3 |
| Immunoprecipitation | 2.5 μg/IP | Fig 4 |
| Immunofluorescence | 1:100 - 1:1,000 | Fig 5 |
* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB
ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB
ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.
Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB
Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB
Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract).
Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB
HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.
NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
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| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| IP Immunoprecipitation Read more |
| Datasheet NFYB C15410241 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: NFYB Antibody (Diagenode Cat# C15410241-100 Lot# 42817). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. 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There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. 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' $meta_title = 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode' $product = array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'modified' => '2022-10-06 11:02:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '405', 'name' => 'NFYB polyclonal antibody - Classic', 'description' => 'NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 1% BSA, 20% glycerol and 0.025% proclin300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-06 10:50:24', 'created' => '2015-07-27 10:14:58', 'select_label' => '405 - NFYB polyclonal antibody - Classic (42817 - 0.42 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ) ), 'Group' => array( 'Group' => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ), 'Master' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'modified' => '2022-10-06 11:02:02', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '564', 'name' => 'Datasheet NFYB C15410241', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYB_C15410241.pdf', 'slug' => 'datasheet-nfyb-c15410241', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/nfyb-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2335', 'antibody_id' => '405', 'name' => 'NFYB polyclonal antibody - Classic', 'description' => '<p><span>Polyclonal antibody raised in rabbit against NFYB (nuclear transcription factor Y, beta), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="ChIP results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="ChIP-seq results figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="ChIP-seq results figure B" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="ChIP-seq results figure C" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="ChIP-seq results figure D" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against NFYB (Cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (Cat. C15410241) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410241-25', 'old_catalog_number' => '', 'sf_code' => 'C15410241-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-25-mg', 'meta_title' => 'NFYB Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IF, IP and WB. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. 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There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. 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' $meta_title = 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode' $product = array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. 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There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 1% BSA, 20% glycerol and 0.025% proclin300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-06 10:50:24', 'created' => '2015-07-27 10:14:58', 'select_label' => '405 - NFYB polyclonal antibody - Classic (42817 - 0.42 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ) ), 'Group' => array( 'Group' => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ), 'Master' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'modified' => '2022-10-06 11:02:02', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '564', 'name' => 'Datasheet NFYB C15410241', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYB_C15410241.pdf', 'slug' => 'datasheet-nfyb-c15410241', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/nfyb-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2335', 'antibody_id' => '405', 'name' => 'NFYB polyclonal antibody - Classic', 'description' => '<p><span>Polyclonal antibody raised in rabbit against NFYB (nuclear transcription factor Y, beta), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="ChIP results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="ChIP-seq results figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="ChIP-seq results figure B" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="ChIP-seq results figure C" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="ChIP-seq results figure D" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against NFYB (Cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (Cat. C15410241) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410241-25', 'old_catalog_number' => '', 'sf_code' => 'C15410241-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-25-mg', 'meta_title' => 'NFYB Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IF, IP and WB. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. 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The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. 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There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 1% BSA, 20% glycerol and 0.025% proclin300.', 'precautions' => 'This product is for research use only. 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' $meta_title = 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode' $product = array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. 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There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 1% BSA, 20% glycerol and 0.025% proclin300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-06 10:50:24', 'created' => '2015-07-27 10:14:58', 'select_label' => '405 - NFYB polyclonal antibody - Classic (42817 - 0.42 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ) ), 'Group' => array( 'Group' => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ), 'Master' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'modified' => '2022-10-06 11:02:02', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '564', 'name' => 'Datasheet NFYB C15410241', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYB_C15410241.pdf', 'slug' => 'datasheet-nfyb-c15410241', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/nfyb-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2335', 'antibody_id' => '405', 'name' => 'NFYB polyclonal antibody - Classic', 'description' => '<p><span>Polyclonal antibody raised in rabbit against NFYB (nuclear transcription factor Y, beta), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="ChIP results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="ChIP-seq results figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="ChIP-seq results figure B" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="ChIP-seq results figure C" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="ChIP-seq results figure D" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against NFYB (Cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (Cat. C15410241) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410241-25', 'old_catalog_number' => '', 'sf_code' => 'C15410241-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-25-mg', 'meta_title' => 'NFYB Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IF, IP and WB. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. 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There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 1% BSA, 20% glycerol and 0.025% proclin300.', 'precautions' => 'This product is for research use only. 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' $meta_title = 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode' $product = array( 'Product' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'modified' => '2022-10-06 11:02:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '405', 'name' => 'NFYB polyclonal antibody - Classic', 'description' => 'NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.', 'clonality' => '', 'isotype' => '', 'lot' => '42817', 'concentration' => '0.42 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2.5 μg/IP</td> <td>Fig 4</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 5</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'PBS containing 1% BSA, 20% glycerol and 0.025% proclin300.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-06 10:50:24', 'created' => '2015-07-27 10:14:58', 'select_label' => '405 - NFYB polyclonal antibody - Classic (42817 - 0.42 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ) ), 'Group' => array( 'Group' => array( 'id' => '62', 'name' => 'C15410241', 'product_id' => '2336', 'modified' => '2016-02-18 21:35:50', 'created' => '2016-02-18 21:35:50' ), 'Master' => array( 'id' => '2336', 'antibody_id' => '405', 'name' => 'NFYB Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410241-100', 'old_catalog_number' => '', 'sf_code' => 'C15410241-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-100-mg', 'meta_title' => 'NFYB Antibody - ChIP-seq Grade (C15410241) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, WB, IP and IF. ', 'modified' => '2022-10-06 11:02:02', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '564', 'name' => 'Datasheet NFYB C15410241', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NFYB_C15410241.pdf', 'slug' => 'datasheet-nfyb-c15410241', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/nfyb-polyclonal-antibody-classic-100-mg' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $pro = array( 'id' => '2335', 'antibody_id' => '405', 'name' => 'NFYB polyclonal antibody - Classic', 'description' => '<p><span>Polyclonal antibody raised in rabbit against NFYB (nuclear transcription factor Y, beta), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="ChIP results" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="ChIP-seq results figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="ChIP-seq results figure B" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="ChIP-seq results figure C" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="ChIP-seq results figure D" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against NFYB (Cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (Cat. C15410241) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 μl', 'catalog_number' => 'C15410241-25', 'old_catalog_number' => '', 'sf_code' => 'C15410241-000032380', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '140', 'price_GBP' => '100', 'price_JPY' => '12600', 'price_CNY' => '', 'price_AUD' => '350', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => false, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nfyb-polyclonal-antibody-classic-25-mg', 'meta_title' => 'NFYB Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NFYB (Nuclear transcription factor Y, beta) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IF, IP and WB. 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1440', 'product_id' => '2336', 'document_id' => '38' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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