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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'name' => 'NFYB polyclonal antibody - Classic',
'description' => '<p><span>Polyclonal antibody raised in rabbit against NFYB (nuclear transcription factor Y, beta), using a recombinant protein.</span></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="ChIP results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="ChIP-seq results figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="ChIP-seq results figure B" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="ChIP-seq results figure C" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="ChIP-seq results figure D" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against NFYB (Cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (Cat. C15410241) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="ChIP results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="ChIP-seq results figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="ChIP-seq results figure B" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="ChIP-seq results figure C" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="ChIP-seq results figure D" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against NFYB (Cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (Cat. C15410241) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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'description' => 'NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.',
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/IP</td>
<td>Fig 1, 2</td>
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<tr>
<td>Western Blotting</td>
<td>1:500 - 1:3,000</td>
<td>Fig 3</td>
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<td>Immunoprecipitation</td>
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<td>Fig 4</td>
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<td>Immunofluorescence</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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'description' => '<p><span>Polyclonal antibody raised in rabbit against<strong> NFYB (nuclear transcription factor Y, beta),</strong> using a recombinant protein.</span></p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
</div>
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<th>Suggested dilution</th>
<th>References</th>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/IP</td>
<td>Fig 1, 2</td>
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<tr>
<td>Western Blotting</td>
<td>1:500 - 1:3,000</td>
<td>Fig 3</td>
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<td>Immunoprecipitation</td>
<td>2.5 μg/IP</td>
<td>Fig 4</td>
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<td>Immunofluorescence</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="NFYB Antibody ChIP Grade" caption="false" width="288" height="217" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="NFYB Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-B.jpg" alt="NFYB Antibody for ChIP-seq" caption="false" width="447" height="122" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-C.jpg" alt="NFYB Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-D.jpg" alt="NFYB Antibody validated in ChIP-seq " caption="false" width="447" height="84" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-WB.jpg" alt="NFYB Antibody validated in Western Blot" caption="false" width="196" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IP.jpg" alt="NFYB Antibody validated in Immunoprecipitation" width="196" height="215" caption="false" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-IFA.jpg" alt="NFYB Antibody validated in Immunofluorescence" caption="false" width="288" height="133" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.</small></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chip.jpg" alt="ChIP results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410241-chipseq-A.jpg" alt="ChIP-seq results figure A" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB</strong><br /> ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes. </small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB</strong><br /> Nuclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against NFYB (Cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB</strong><br /> Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract). </small></p>
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<p><small><strong> Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB</strong><br /> HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (Cat. C15410241) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p>
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