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Monoclonal antibody raised in mouse against human NRF1 (Nuclear Respiratory Factor 1), using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 2.2 μg/μl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 2 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:1,000 | Fig 3 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.
Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1
ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1
ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).
Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1
Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-qPCR (ab) Read more |
| NRF1 monoclonal antibody DATASHEET Datasheet of the NRF1 monoclonal antibody | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: NRF1 Antibody (sample size) (Diagenode Cat# C15200013-10 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Batch-specific data available on the website. Sample size available.' $meta_title = 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode' $product = array( 'Product' => array( 'id' => '2937', 'antibody_id' => '466', 'name' => 'NRF1 Antibody (sample size)', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'nrf1-monoclonal-antibody-classic', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-09 16:49:58', 'created' => '2015-12-02 11:18:58', 'select_label' => '466 - NRF1 monoclonal antibody - Classic (001 - 2.2 μg/μl - Human - Protein A purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '236', 'name' => 'C15200013', 'product_id' => '2770', 'modified' => '2018-01-08 12:48:17', 'created' => '2018-01-08 12:48:17' ), 'Master' => array( 'id' => '2770', 'antibody_id' => '466', 'name' => 'NRF1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15200013', 'old_catalog_number' => '', 'sf_code' => 'C15200013-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nrf1-monoclonal-antibody-classic', 'meta_title' => 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode', 'meta_keywords' => 'Monoclonal antibody,NRF1 monoclonal antibody,ChIP-seq', 'meta_description' => 'NRF1 (Nuclear Respiratory Factor 1) Monoclonal Antibody validated in WB, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '861', 'name' => 'NRF1 monoclonal antibody', 'description' => '<p>Datasheet of the NRF1 monoclonal antibody</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_C15200013_NRF1.pdf', 'slug' => 'nrf1-monoclonal-antibody-C15200013', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-12-02 16:55:34', 'created' => '2015-12-02 16:35:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '1238', 'name' => 'NRF1 antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1240', 'name' => 'NRF1 antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2021-08-27 16:00:00', 'created' => '2021-08-27 16:00:00', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1235', 'name' => 'NRF1 antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1239', 'name' => 'NRF1 antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1234', 'name' => 'NRF1 antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1233', 'name' => 'NRF1 antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1237', 'name' => 'NRF1 antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1236', 'name' => 'NRF1 antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/nrf1-monoclonal-antibody-classic' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2770', 'antibody_id' => '466', 'name' => 'NRF1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. 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ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. 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ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'NRF1 (Nuclear Respiratory Factor 1) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode' $product = array( 'Product' => array( 'id' => '2937', 'antibody_id' => '466', 'name' => 'NRF1 Antibody (sample size)', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'nrf1-monoclonal-antibody-classic', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-09 16:49:58', 'created' => '2015-12-02 11:18:58', 'select_label' => '466 - NRF1 monoclonal antibody - Classic (001 - 2.2 μg/μl - Human - Protein A purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '236', 'name' => 'C15200013', 'product_id' => '2770', 'modified' => '2018-01-08 12:48:17', 'created' => '2018-01-08 12:48:17' ), 'Master' => array( 'id' => '2770', 'antibody_id' => '466', 'name' => 'NRF1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. 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ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '861', 'name' => 'NRF1 monoclonal antibody', 'description' => '<p>Datasheet of the NRF1 monoclonal antibody</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_C15200013_NRF1.pdf', 'slug' => 'nrf1-monoclonal-antibody-C15200013', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-12-02 16:55:34', 'created' => '2015-12-02 16:35:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '1238', 'name' => 'NRF1 antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1240', 'name' => 'NRF1 antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2021-08-27 16:00:00', 'created' => '2021-08-27 16:00:00', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1235', 'name' => 'NRF1 antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1239', 'name' => 'NRF1 antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1234', 'name' => 'NRF1 antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1233', 'name' => 'NRF1 antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1237', 'name' => 'NRF1 antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1236', 'name' => 'NRF1 antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/nrf1-monoclonal-antibody-classic' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2770', 'antibody_id' => '466', 'name' => 'NRF1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. 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ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. 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ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode' $product = array( 'Product' => array( 'id' => '2937', 'antibody_id' => '466', 'name' => 'NRF1 Antibody (sample size)', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. 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Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. 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ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. 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Sample size available.', 'modified' => '2021-12-23 12:23:07', 'created' => '2015-12-02 15:28:00' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '861', 'name' => 'NRF1 monoclonal antibody', 'description' => '<p>Datasheet of the NRF1 monoclonal antibody</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_C15200013_NRF1.pdf', 'slug' => 'nrf1-monoclonal-antibody-C15200013', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-12-02 16:55:34', 'created' => '2015-12-02 16:35:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '1238', 'name' => 'NRF1 antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1240', 'name' => 'NRF1 antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2021-08-27 16:00:00', 'created' => '2021-08-27 16:00:00', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1235', 'name' => 'NRF1 antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1239', 'name' => 'NRF1 antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1234', 'name' => 'NRF1 antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1233', 'name' => 'NRF1 antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1237', 'name' => 'NRF1 antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1236', 'name' => 'NRF1 antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/nrf1-monoclonal-antibody-classic' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2770', 'antibody_id' => '466', 'name' => 'NRF1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15200013', 'old_catalog_number' => '', 'sf_code' => 'C15200013-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nrf1-monoclonal-antibody-classic', 'meta_title' => 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode', 'meta_keywords' => 'Monoclonal antibody,NRF1 monoclonal antibody,ChIP-seq', 'meta_description' => 'NRF1 (Nuclear Respiratory Factor 1) Monoclonal Antibody validated in WB, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. 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ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. 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ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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Batch-specific data available on the website. Sample size available.' $meta_title = 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode' $product = array( 'Product' => array( 'id' => '2937', 'antibody_id' => '466', 'name' => 'NRF1 Antibody (sample size)', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15200013', 'old_catalog_number' => '', 'sf_code' => 'C15200013-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nrf1-monoclonal-antibody-classic', 'meta_title' => 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode', 'meta_keywords' => 'Monoclonal antibody,NRF1 monoclonal antibody,ChIP-seq', 'meta_description' => 'NRF1 (Nuclear Respiratory Factor 1) Monoclonal Antibody validated in WB, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. Sample size available.', 'modified' => '2021-12-23 12:23:07', 'created' => '2015-12-02 15:28:00' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '861', 'name' => 'NRF1 monoclonal antibody', 'description' => '<p>Datasheet of the NRF1 monoclonal antibody</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_C15200013_NRF1.pdf', 'slug' => 'nrf1-monoclonal-antibody-C15200013', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-12-02 16:55:34', 'created' => '2015-12-02 16:35:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '1238', 'name' => 'NRF1 antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1240', 'name' => 'NRF1 antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2021-08-27 16:00:00', 'created' => '2021-08-27 16:00:00', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1235', 'name' => 'NRF1 antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1239', 'name' => 'NRF1 antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1234', 'name' => 'NRF1 antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1233', 'name' => 'NRF1 antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '1237', 'name' => 'NRF1 antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '1236', 'name' => 'NRF1 antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-08-27 15:06:59', 'created' => '2021-08-27 15:06:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/nrf1-monoclonal-antibody-classic' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2770', 'antibody_id' => '466', 'name' => 'NRF1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein. </p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15200013', 'old_catalog_number' => '', 'sf_code' => 'C15200013-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'nrf1-monoclonal-antibody-classic', 'meta_title' => 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode', 'meta_keywords' => 'Monoclonal antibody,NRF1 monoclonal antibody,ChIP-seq', 'meta_description' => 'NRF1 (Nuclear Respiratory Factor 1) Monoclonal Antibody validated in WB, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. Sample size available.', 'modified' => '2021-12-23 12:23:07', 'created' => '2015-12-02 15:28:00' ) ) $pro = array( 'id' => '2937', 'antibody_id' => '466', 'name' => 'NRF1 Antibody (sample size)', 'description' => '', 'label1' => 'Validation data', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200013-10', 'old_catalog_number' => '', 'sf_code' => 'C15200013-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '0', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'nrf1-monoclonal-antibody-classic-10-ug', 'meta_title' => 'NRF1 Antibody - ChIP-seq Grade (C15200013) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NRF1 (Nuclear Respiratory Factor 1) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. 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ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. 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