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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
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<td>Fig 1, 2</td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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