PAPOLA Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15310137
(CS-137-100)

100 µl

$260.00
Bulk order

Alternative names: PAP, PAP-alpha

Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.

Lot	A427-001
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100 - 1:500	Fig 1
Western Blotting	1:1,000	Fig 2

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.

Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA
Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.

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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA</strong><br />Nuclear extracts from HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.</small></p>
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			'modified' => '2023-01-10 11:38:24',
			'created' => '2023-01-10 11:38:24',
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			'url' => 'files/SDS/PAPOLA/SDS-C15310137-PAPOLA_antibody-BE-nl-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2023-01-10 11:38:24',
			'created' => '2023-01-10 11:38:24',
			'ProductsSafetySheet' => array(
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		(int) 5 => array(
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			'name' => 'PAPOLA antibody SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/PAPOLA/SDS-C15310137-PAPOLA_antibody-BE-fr-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2023-01-10 11:38:24',
			'created' => '2023-01-10 11:38:24',
			'ProductsSafetySheet' => array(
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			'modified' => '2023-01-10 11:38:24',
			'created' => '2023-01-10 11:38:24',
			'ProductsSafetySheet' => array(
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$other_formats = array()
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$testimonials = ''
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$related_products = ''
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$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
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	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'online' => true,
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	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
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		'application_id' => '19'
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$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
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	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
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$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
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	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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	'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
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	'meta_description' => '',
	'modified' => '2016-06-15 11:24:06',
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	'url' => 'files/SDS/PAPOLA/SDS-C15310137-PAPOLA_antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2023-01-10 11:38:24',
	'created' => '2023-01-10 11:38:24',
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		'product_id' => '2124',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
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	'meta_keywords' => '',
	'meta_description' => 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha',
	'meta_title' => 'PAPOLA Polyclonal Antibody | Diagenode',
	'product' => array(
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			'antibody_id' => '186',
			'name' => 'PAPOLA Antibody ',
			'description' => '<p><span>Alternative names: <strong>PAP</strong>, <strong>PAP-alpha</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>',
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			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig1.jpg" alt="ELISA" height="244" width="303" /><br /><br /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig2.jpg" alt="Western blot" height="115" width="104" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA</strong><br />Nuclear extracts from HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.</small></p>
</div>
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			'locale' => 'eng'
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<th>Suggested dilution</th>
<th>References</th>
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<td>1:100 - 1:500</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
</tbody>
</table>',
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			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2016-08-03 11:52:08',
			'created' => '0000-00-00 00:00:00',
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$meta_description = 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha'
$meta_title = 'PAPOLA Polyclonal Antibody | Diagenode'
$product = array(
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		'antibody_id' => '186',
		'name' => 'PAPOLA Antibody ',
		'description' => '<p><span>Alternative names: <strong>PAP</strong>, <strong>PAP-alpha</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig1.jpg" alt="ELISA" height="244" width="303" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig2.jpg" alt="Western blot" height="115" width="104" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA</strong><br />Nuclear extracts from HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.</small></p>
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		'slug' => 'papola-polyclonal-antibody-pioneer-100-ul',
		'meta_title' => 'PAPOLA Polyclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha',
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		'concentration' => 'not determined',
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<th>Suggested dilution</th>
<th>References</th>
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<td>ELISA</td>
<td>1:100 - 1:500</td>
<td>Fig 1</td>
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<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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</tbody>
</table>',
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'meta_description' => '',
		'modified' => '2016-08-03 11:52:08',
		'created' => '0000-00-00 00:00:00',
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
			'meta_title' => 'Transcription factor Antibodies | Diagenode',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'created' => '2015-11-02 14:49:22',
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			'description' => 'Datasheet description',
			'image_id' => null,
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			'name' => 'Antibodies you can trust',
			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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			'type' => 'Poster',
			'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
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			'modified' => '2015-10-01 20:18:31',
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			'name' => 'Epigenetic Antibodies Brochure',
			'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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			'created' => '2023-01-10 11:38:24',
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	(int) 3 => 'GB',
	(int) 4 => 'DK',
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$other_formats = array()
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)
$chipseq_service = array(
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$application = array(
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	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'slug' => 'western-blot-antibodies',
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	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'created' => '2015-01-07 09:20:00',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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	'meta_description' => 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha',
	'meta_title' => 'PAPOLA Polyclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
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			'antibody_id' => '186',
			'name' => 'PAPOLA Antibody ',
			'description' => '<p><span>Alternative names: <strong>PAP</strong>, <strong>PAP-alpha</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig1.jpg" alt="ELISA" height="244" width="303" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig2.jpg" alt="Western blot" height="115" width="104" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA</strong><br />Nuclear extracts from HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.</small></p>
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			'slug' => 'papola-polyclonal-antibody-pioneer-100-ul',
			'meta_title' => 'PAPOLA Polyclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha',
			'modified' => '2022-01-05 15:54:55',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
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			'id' => '186',
			'name' => 'PAPOLA polyclonal antibody',
			'description' => 'PAPOLA (UniProtKB/Swiss-Prot entry P51003) is an RNA polymerase that generates the poly(A) tail of RNA’s by adding AMP monomers to the 3’-end. PAPOLA may also be required for the endoribonucleolytic cleavage reaction at some polyadenylation sites.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A427-001',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Pioneer',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
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<tbody>
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<td>ELISA</td>
<td>1:100 - 1:500</td>
<td>Fig 1</td>
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<td>Fig 2</td>
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA</strong><br />Nuclear extracts from HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.</small></p>
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	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '38',
	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
	'image_id' => null,
	'type' => 'Brochure',
	'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
	'slug' => 'epigenetic-antibodies-brochure',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2016-06-15 11:24:06',
	'created' => '2015-07-03 16:05:27',
	'ProductsDocument' => array(
		'id' => '1667',
		'product_id' => '2124',
		'document_id' => '38'
	)
)
$sds = array(
	'id' => '2940',
	'name' => 'PAPOLA antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/PAPOLA/SDS-C15310137-PAPOLA_antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2023-01-10 11:38:24',
	'created' => '2023-01-10 11:38:24',
	'ProductsSafetySheet' => array(
		'id' => '4828',
		'product_id' => '2124',
		'safety_sheet_id' => '2940'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha',
	'meta_title' => 'PAPOLA Polyclonal Antibody | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2124',
			'antibody_id' => '186',
			'name' => 'PAPOLA Antibody ',
			'description' => '<p><span>Alternative names: <strong>PAP</strong>, <strong>PAP-alpha</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig1.jpg" alt="ELISA" height="244" width="303" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig2.jpg" alt="Western blot" height="115" width="104" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA</strong><br />Nuclear extracts from HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '100 µl',
			'catalog_number' => 'C15310137',
			'old_catalog_number' => 'CS-137-100',
			'sf_code' => 'C15310137-D001-001161',
			'type' => 'FRE',
			'search_order' => '03-Antibody',
			'price_EUR' => '260',
			'price_USD' => '260',
			'price_GBP' => '245',
			'price_JPY' => '40730',
			'price_CNY' => '',
			'price_AUD' => '650',
			'country' => 'ALL',
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			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'papola-polyclonal-antibody-pioneer-100-ul',
			'meta_title' => 'PAPOLA Polyclonal Antibody | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha',
			'modified' => '2022-01-05 15:54:55',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => '186',
			'name' => 'PAPOLA polyclonal antibody',
			'description' => 'PAPOLA (UniProtKB/Swiss-Prot entry P51003) is an RNA polymerase that generates the poly(A) tail of RNA’s by adding AMP monomers to the 3’-end. PAPOLA may also be required for the endoribonucleolytic cleavage reaction at some polyadenylation sites.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A427-001',
			'concentration' => 'not determined',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Pioneer',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:100 - 1:500</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
</tbody>
</table>',
			'storage_conditions' => '',
			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2016-08-03 11:52:08',
			'created' => '0000-00-00 00:00:00',
			'select_label' => '186 - PAPOLA polyclonal antibody (A427-001 - not determined - Human - Whole antiserum - Rabbit)'
		),
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		'Group' => array(),
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		'Application' => array(
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		'Image' => array(
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		'SafetySheet' => array(
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$meta_keywords = ''
$meta_description = 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha'
$meta_title = 'PAPOLA Polyclonal Antibody | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2124',
		'antibody_id' => '186',
		'name' => 'PAPOLA Antibody ',
		'description' => '<p><span>Alternative names: <strong>PAP</strong>, <strong>PAP-alpha</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.</span></p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig1.jpg" alt="ELISA" height="244" width="303" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310137_fig2.jpg" alt="Western blot" height="115" width="104" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA</strong><br />Nuclear extracts from HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
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		'sf_code' => 'C15310137-D001-001161',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '260',
		'price_USD' => '260',
		'price_GBP' => '245',
		'price_JPY' => '40730',
		'price_CNY' => '',
		'price_AUD' => '650',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'papola-polyclonal-antibody-pioneer-100-ul',
		'meta_title' => 'PAPOLA Polyclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'PAPOLA (PolyA polymerase alpha) Polyclonal Antibody  validated  in  WB and ELISA.  Batch-specific data available on the website. Alternative names: PAP, PAP-alpha',
		'modified' => '2022-01-05 15:54:55',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '186',
		'name' => 'PAPOLA polyclonal antibody',
		'description' => 'PAPOLA (UniProtKB/Swiss-Prot entry P51003) is an RNA polymerase that generates the poly(A) tail of RNA’s by adding AMP monomers to the 3’-end. PAPOLA may also be required for the endoribonucleolytic cleavage reaction at some polyadenylation sites.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A427-001',
		'concentration' => 'not determined',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Whole antiserum',
		'classification' => 'Pioneer',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:100 - 1:500</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2016-08-03 11:52:08',
		'created' => '0000-00-00 00:00:00',
		'select_label' => '186 - PAPOLA polyclonal antibody (A427-001 - not determined - Human - Whole antiserum - Rabbit)'
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(),
	'Application' => array(
		(int) 0 => array(
			'id' => '20',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'ELISA',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
			'created' => '2014-07-08 08:13:28',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
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			'position' => '50',
			'parent_id' => '4',
			'name' => 'Transcription',
			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
</ul>',
			'no_promo' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'transcription-factor',
			'cookies_tag_id' => null,
			'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies',
			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
			'meta_title' => 'Transcription factor Antibodies | Diagenode',
			'modified' => '2020-07-06 12:59:19',
			'created' => '2015-03-12 10:20:08',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
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			),
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			'name' => 'Datasheet PAPOLA CS-137-100',
			'description' => 'Datasheet description',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_PAPOLA_CS-137-100.pdf',
			'slug' => 'datasheet-papola-cs-137-100',
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => '2015-07-07 11:47:45',
			'created' => '2015-07-07 11:47:45',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '11',
			'name' => 'Antibodies you can trust',
			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
			'image_id' => null,
			'type' => 'Poster',
			'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
			'slug' => 'antibodies-you-can-trust-poster',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-10-01 20:18:31',
			'created' => '2015-07-03 16:05:15',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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Datasheet PAPOLA CS-137-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

PAPOLA antibody SDS GB en	Download
PAPOLA antibody SDS US en	Download
PAPOLA antibody SDS DE de	Download
PAPOLA antibody SDS JP ja	Download
PAPOLA antibody SDS BE nl	Download
PAPOLA antibody SDS BE fr	Download
PAPOLA antibody SDS FR fr	Download
PAPOLA antibody SDS ES es	Download

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