Diagenode

PAPOLA polyclonal antibody

目录号
格式
价格
C15310137
(CS-137-100)
100 µl
$260.00
  Bulk order



Alternative names: PAP, PAP-alpha

Polyclonal antibody raised in rabbit against human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.

LotA427-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:500 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPOLA (Cat. No. CS-137-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22,500.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against PAPOLA
    Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPOLA (Cat. No. CS-137-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.

  •  应用
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  文档
    Datasheet PAPOLA CS-137-100 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Safety sheets
    PAPOLA antibody SDS GB en Download
    PAPOLA antibody SDS US en Download
    PAPOLA antibody SDS DE de Download
    PAPOLA antibody SDS JP ja Download
    PAPOLA antibody SDS BE nl Download
    PAPOLA antibody SDS BE fr Download
    PAPOLA antibody SDS FR fr Download
    PAPOLA antibody SDS ES es Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PAPOLA polyclonal antibody (Diagenode Cat# C15310137 Lot# A427-001). Click here to copy to clipboard.

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