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Figure 1. ChIP RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune mouse IgG B. 5 μg of RbAp48 antibody (C15200206) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
Figure 2. Immunoprecipitation RbAp48 antibody immunoprecipitates RbAp48 protein in IP experiments. IP Sample: 1,000 μg HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2.5 μg of preimmune mouse IgG C. Immunoprecipitation of RbAp48 protein with 2.5 μg of RbAp48 antibody (C15200206) The immunoprecipitated RbAp48 protein was detected by western blot with the RbAp48 antibody diluted 1:500.
Figure 3. Immunofluorescent analysis Immunofluorescence analysis of HeLa cells, using RbAp48 (Cat. No. C15200206) antibody at a 1:100 dilution.
Figure 4. Western blot Detection of RbAp48 in HeLa nuclear extract.
IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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