| Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico Protein extraction from tissues and cultured cells is the first step for many biochemical and ana... | Download |
The 15 ml Bioruptor® Tubes strongly improve protein extraction efficiency. The tubes have been thoroughly validated for use on the Bioruptor® Pico and are strongly recommended for extraction of proteins using this instrument. Recommended sample volume: 500 μl - 2 ml.
| Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico Protein extraction from tissues and cultured cells is the first step for many biochemical and ana... | Download |
| 15 ml Bioruptor® Tubes DATASHEET The 15 ml Bioruptor® Tubes C30010017 strongly improve protein extraction efficie... | Download |
| Which tubes for which Bioruptor®? QUICK GUIDE Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu... | Download |
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var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2024-06-14 15:28:50', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha673ef16cab9df"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3046" class="alert button expand" form="Quote-3046" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3046 #state-3046").parent().parent().show(); } else { $("#Quote-3046 #state-3046").parent().parent().hide(); $("#Quote-3046 #state-3046").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3046" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptorpico2" data-reveal-id="quoteModal-3046" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Pico sonication device</h6> </div> </div> </li> ' $related = array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 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Recommended sample volume: 500 μl - 2 ml.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '50 pc', 'catalog_number' => 'C30010017', 'old_catalog_number' => '', 'sf_code' => 'C30010017-1860', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '75', 'price_USD' => '115', 'price_GBP' => '65', 'price_JPY' => '11750', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-bioruptor-tubes-50-pc', 'meta_title' => '15 ml Bioruptor® Tubes', 'meta_keywords' => '', 'meta_description' => '15 ml Bioruptor® Tubes', 'modified' => '2022-01-25 04:02:13', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>', 'label1' => 'Characteristics', 'info1' => '<p><span>The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction </span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p> <ul> <li>Fast and simple</li> <li>No contamination between samples</li> <li>Efficient</li> <li>Gentle processing</li> <li>Reproducible</li> <li>Temperature controlled</li> <li>Multiplexing capability</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 g', 'catalog_number' => 'C20000021', 'old_catalog_number' => 'AL-003-0020', 'sf_code' => 'C20000021-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '95', 'price_USD' => '100', 'price_GBP' => '90', 'price_JPY' => '14880', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'protein-extraction-beads-20-g', 'meta_title' => 'Protein Extraction beads', 'meta_keywords' => '', 'meta_description' => 'Protein Extraction beads', 'modified' => '2020-05-27 14:42:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '1 pack', 'catalog_number' => 'B01200016', 'old_catalog_number' => 'O-ring-15', 'sf_code' => 'B01200016-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '300', 'price_USD' => '350', 'price_GBP' => '250', 'price_JPY' => '46995', 'price_CNY' => '', 'price_AUD' => '875', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack', 'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'meta_keywords' => '', 'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'modified' => '2022-01-25 04:00:28', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2024-06-14 15:28:50', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha673ef16cab9df"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3046" class="alert button expand" form="Quote-3046" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3046 #state-3046").parent().parent().show(); } else { $("#Quote-3046 #state-3046").parent().parent().hide(); $("#Quote-3046 #state-3046").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3046" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptorpico2" data-reveal-id="quoteModal-3046" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Pico sonication device</h6> </div> </div> </li> ' $related = array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 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Recommended sample volume: 500 μl - 2 ml.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '50 pc', 'catalog_number' => 'C30010017', 'old_catalog_number' => '', 'sf_code' => 'C30010017-1860', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '75', 'price_USD' => '115', 'price_GBP' => '65', 'price_JPY' => '11750', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-bioruptor-tubes-50-pc', 'meta_title' => '15 ml Bioruptor® Tubes', 'meta_keywords' => '', 'meta_description' => '15 ml Bioruptor® Tubes', 'modified' => '2022-01-25 04:02:13', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>', 'label1' => 'Characteristics', 'info1' => '<p><span>The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction </span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p> <ul> <li>Fast and simple</li> <li>No contamination between samples</li> <li>Efficient</li> <li>Gentle processing</li> <li>Reproducible</li> <li>Temperature controlled</li> <li>Multiplexing capability</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 g', 'catalog_number' => 'C20000021', 'old_catalog_number' => 'AL-003-0020', 'sf_code' => 'C20000021-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '95', 'price_USD' => '100', 'price_GBP' => '90', 'price_JPY' => '14880', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'protein-extraction-beads-20-g', 'meta_title' => 'Protein Extraction beads', 'meta_keywords' => '', 'meta_description' => 'Protein Extraction beads', 'modified' => '2020-05-27 14:42:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '1 pack', 'catalog_number' => 'B01200016', 'old_catalog_number' => 'O-ring-15', 'sf_code' => 'B01200016-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '300', 'price_USD' => '350', 'price_GBP' => '250', 'price_JPY' => '46995', 'price_CNY' => '', 'price_AUD' => '875', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack', 'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'meta_keywords' => '', 'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'modified' => '2022-01-25 04:00:28', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2024-06-14 15:28:50', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha673ef16cab9df"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3046" class="alert button expand" form="Quote-3046" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3046 #state-3046").parent().parent().show(); } else { $("#Quote-3046 #state-3046").parent().parent().hide(); $("#Quote-3046 #state-3046").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3046" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptorpico2" data-reveal-id="quoteModal-3046" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Pico sonication device</h6> </div> </div> </li> ' $related = array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 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Recommended sample volume: 500 μl - 2 ml.</span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '50 pc', 'catalog_number' => 'C30010017', 'old_catalog_number' => '', 'sf_code' => 'C30010017-1860', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '75', 'price_USD' => '115', 'price_GBP' => '65', 'price_JPY' => '11750', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-bioruptor-tubes-50-pc', 'meta_title' => '15 ml Bioruptor® Tubes', 'meta_keywords' => '', 'meta_description' => '15 ml Bioruptor® Tubes', 'modified' => '2022-01-25 04:02:13', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>', 'label1' => 'Characteristics', 'info1' => '<p><span>The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction </span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p> <ul> <li>Fast and simple</li> <li>No contamination between samples</li> <li>Efficient</li> <li>Gentle processing</li> <li>Reproducible</li> <li>Temperature controlled</li> <li>Multiplexing capability</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 g', 'catalog_number' => 'C20000021', 'old_catalog_number' => 'AL-003-0020', 'sf_code' => 'C20000021-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '95', 'price_USD' => '100', 'price_GBP' => '90', 'price_JPY' => '14880', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'protein-extraction-beads-20-g', 'meta_title' => 'Protein Extraction beads', 'meta_keywords' => '', 'meta_description' => 'Protein Extraction beads', 'modified' => '2020-05-27 14:42:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '1 pack', 'catalog_number' => 'B01200016', 'old_catalog_number' => 'O-ring-15', 'sf_code' => 'B01200016-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '300', 'price_USD' => '350', 'price_GBP' => '250', 'price_JPY' => '46995', 'price_CNY' => '', 'price_AUD' => '875', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack', 'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'meta_keywords' => '', 'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'modified' => '2022-01-25 04:00:28', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2024-06-14 15:28:50', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha673ef16cab9df"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3046" class="alert button expand" form="Quote-3046" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3046 #state-3046").parent().parent().show(); } else { $("#Quote-3046 #state-3046").parent().parent().hide(); $("#Quote-3046 #state-3046").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3046" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptorpico2" data-reveal-id="quoteModal-3046" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Pico sonication device</h6> </div> </div> </li> ' $related = array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 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