Total RNA sequencing (RNA-Seq) detects both coding and noncoding RNAs and is typically used to measure gene and transcript abundance as well as to identify novel components of the transcriptome. Messenger RNA-Seq focuses on the quantification of gene expression, the identification of unknown transcripts, the discovery of alternative splicing and gene fusion events. And finally, small non-coding RNA sequencing (sncRNA-Seq) will detect small (<100 nucleotides long) RNAs that operate as key regulators in cellular processes.
This analysis provides information for either genes or isoforms with their expression levels.
If you require a type of analysis that is not in the previous list, please consult with our expert bioinformatics team.
MGcount: Diagenode has developed a bioinformatics software for counting whole-transcriptome RNA-seq reads from one or more input alignment files. It is specially designed to incorporate multi-mapping and multi-overlapping reads in the quantification using a flexible methodology that is compatible with any biotype. At the end of its execution, it produces a count matrix, compatible with any downstream analysis.
 How to properly cite our product/service in your workWe strongly recommend using this: RNA Data Analysis (Hologic Diagenode Cat# G02030005). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
MGcount: a total RNA-seq quantification tool to address multi-mappingand multi-overlapping alignments ambiguity in non-coding transcripts |
