Figure 2. Western blot analysis using the Diagenode antibody directed against SPI1
Western blot was performed using and the Diagenode antibody against mouse SPI1 (Cat. No. CS-120-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Figure 2 shows the results for mouse fibroblasts (3T3, lane 1), macrophages (RAW, lane 2) and pre-B cells (HAFTL, lane 3). The position of the protein of interest (expected size: 31 kDa) is indicated on the left. Fibroblasts do not express SPI1, whereas macrophages show a high and pre-B cells a low expression of SPI1.
Western blot performed by Maribel Parra, Center for Genomic Regulation, Barcelona, Spain.