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<p><span>Polyclonal antibody raised in rabbit against mouse SPI1 (spleen focus forming virus (SFFV) proviral integration oncogene), using two KLH-conjugated synthetic peptides containing a sequence from the N-terminus and from the C-terminus of the protein, respectively.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse SPI1 (Cat. No. CS-120-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,600.</small></p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/SPI1-wb.png" /></center></div>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SPI1</strong><br />Western blot was performed using and the Diagenode antibody against mouse SPI1 (Cat. No. CS-120-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Figure 2 shows the results for mouse fibroblasts (3T3, lane 1), macrophages (RAW, lane 2) and pre-B cells (HAFTL, lane 3). The position of the protein of interest (expected size: 31 kDa) is indicated on the left. Fibroblasts do not express SPI1, whereas macrophages show a high and pre-B cells a low expression of SPI1.<br /> Western blot performed by Maribel Parra, Center for Genomic Regulation, Barcelona, Spain.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse SPI1 (Cat. No. CS-120-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,600.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SPI1</strong><br />Western blot was performed using and the Diagenode antibody against mouse SPI1 (Cat. No. CS-120-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Figure 2 shows the results for mouse fibroblasts (3T3, lane 1), macrophages (RAW, lane 2) and pre-B cells (HAFTL, lane 3). The position of the protein of interest (expected size: 31 kDa) is indicated on the left. Fibroblasts do not express SPI1, whereas macrophages show a high and pre-B cells a low expression of SPI1.<br /> Western blot performed by Maribel Parra, Center for Genomic Regulation, Barcelona, Spain.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against SPI1</strong><br />Western blot was performed using and the Diagenode antibody against mouse SPI1 (Cat. No. CS-120-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Figure 2 shows the results for mouse fibroblasts (3T3, lane 1), macrophages (RAW, lane 2) and pre-B cells (HAFTL, lane 3). The position of the protein of interest (expected size: 31 kDa) is indicated on the left. Fibroblasts do not express SPI1, whereas macrophages show a high and pre-B cells a low expression of SPI1.<br /> Western blot performed by Maribel Parra, Center for Genomic Regulation, Barcelona, Spain.</small></p>
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$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
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(int) 0 => 'western-blot-antibodies'
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'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
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$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
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'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
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'product_id' => '2110',
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)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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