Alternative names: PRKCBP1, RACK7, PRO2893
Polyclonal antibody raised in rabbit against human ZMYND8 (zinc finger, MYND-type containing 8), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
| Lot | A331-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:500 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ZMYND8 (Cat. No. CS-136-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:36,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against ZMYND8
Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against ZMYND8 (Cat. No. CS-136-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 132 kDa); the marker (in kDa) is shown on the left.
 How to properly cite our product/service in your workWe strongly recommend using this: ZMYND8 Antibody (Hologic Diagenode Cat# C15310136 Lot# A331-004). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
