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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ZMYND8 (Cat. No. CS-136-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:36,000.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ZMYND8</strong><br />Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against ZMYND8 (Cat. No. CS-136-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 132 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ZMYND8 (Cat. No. CS-136-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:36,000.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ZMYND8</strong><br />Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against ZMYND8 (Cat. No. CS-136-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 132 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310136_fig1.jpg" alt="ELISA" height="288" width="366" /><br /><br /></p>
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ZMYND8 (Cat. No. CS-136-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:36,000.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310136_fig2.jpg" alt="Western blot" height="106" width="108" /></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ZMYND8</strong><br />Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against ZMYND8 (Cat. No. CS-136-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 132 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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