Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells Published: August, 2020 Abstract:
Chronic exposure to inorganic arsenic is associated with a variety of adverse health effects, including lung, bladder, kidney, and liver cancer. Several mechanisms have been proposed for arsenic-induced tumorigenesis; however, insufficient knowled... Read more »
Corticosteroid receptors adopt distinct cyclical transcriptional signatures Published: March, 2018 Abstract:
Mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs) are two closely related hormone-activated transcription factors that regulate major pathophysiologic functions. High homology between these receptors accounts for the crossbindin... Read more »
Corticosteroid receptors adopt distinct cyclical transcriptional signatures Published: March, 2018 Abstract:
Mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs) are two closely related hormone-activated transcription factors that regulate major pathophysiologic functions. High homology between these receptors accounts for the crossbindin... Read more »
MYC drives overexpression of telomerase RNA (hTR/TERC) in prostate cancer Published: September, 2017 Abstract:
Telomerase consists of at least two essential elements, an RNA component hTR or TERC that contains the template for telomere DNA addition, and a catalytic reverse transcriptase (TERT). While expression of TERT has been considered the key rate limi... Read more »
The complex genetics of hypoplastic left heart syndrome Published: July, 2017 Abstract:
Congenital heart disease (CHD) affects up to 1% of live births. Although a genetic etiology is indicated by an increased recurrence risk, sporadic occurrence suggests that CHD genetics is complex. Here, we show that hypoplastic left heart syndrome... Read more »
Crebbp loss cooperates with Bcl2 over-expression to promote lymphoma in mice Published: March, 2017 Abstract:
CREBBP is targeted by inactivating mutations in follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL). Here, we provide evidence from transgenic mouse models that Crebbp deletion results in deficits in B-cell development and can coope... Read more »
Osterix and RUNX2 are Transcriptional Regulators of Sclerostin in Human Bone Published: May, 2016 Abstract:
Sclerostin, encoded by the SOST gene, works as an inhibitor of the Wnt pathway and therefore is an important regulator of bone homeostasis. Due to its potent action as an inhibitor of bone formation, blocking sclerostin activity is the purpose of ... Read more »
Interaction with WDR5 Promotes Target Gene Recognition and Tumorigenesis by MYC. Published: May, 2015 Abstract:
MYC is an oncoprotein transcription factor that is overexpressed in the majority of malignancies. The oncogenic potential of MYC stems from its ability to bind regulatory sequences in thousands of target genes, which depends on interaction of MYC ... Read more »
C2H2 zinc finger proteins greatly expand the human regulatory lexicon. Published: May, 2015 Abstract:
Cys2-His2 zinc finger (C2H2-ZF) proteins represent the largest class of putative human transcription factors. However, for most C2H2-ZF proteins it is unknown whether they even bind DNA or, if they do, to which sequences. Here, by combining data f... Read more »
Global regulation of heterochromatin spreading by Leo1. Published: May, 2015 Abstract:
Heterochromatin plays important roles in eukaryotic genome regulation. However, the repressive nature of heterochromatin combined with its propensity to self-propagate necessitates robust mechanisms to contain heterochromatin within defined bounda... Read more »
Targeting the MLL complex in castration-resistant prostate cancer. Published: April, 2015 Abstract:
Resistance to androgen deprivation therapies and increased androgen receptor (AR) activity are major drivers of castration-resistant prostate cancer (CRPC). Although prior work has focused on targeting AR directly, co-activators of AR signaling, w... Read more »
Bacterial Infection Remodels the DNA Methylation Landscape of Human Dendritic Cells Published: March, 2015 Abstract:
DNA methylation is thought to be robust to environmental perturbations on a short time scale. Here, we challenge that view by demonstrating that the infection of human dendritic cells (DCs) with a pathogenic bacteria is associated with rapid chang... Read more »
The DREAM complex promotes gene body H2A.Z for target repression. Published: March, 2015 Abstract:
The DREAM (DP, Retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell cycle genes, but its mechanism of action is poorly understood. Here we show that Caenorhabditis elegans DREAM targets have an unusual p... Read more »
Genome-wide redistribution of BRD4 binding sites in transformation resistant cells. Published: March, 2015 Abstract:
Hutchinson-Gilford progeria syndrome (HGPS) patients do not develop cancer despite a significant accumulation of DNA damage in their cells. We have recently reported that HGPS cells are refractory to experimental oncogenic transformation and we id... Read more »
Dynamic Regulation of Schwann Cell Enhancers after Peripheral Nerve Injury. Published: January, 2015 Abstract:
Myelination of the peripheral nervous system is required for axonal function and long term stability. After peripheral nerve injury, Schwann cells transition from axon myelination to a demyelinated state that supports neuronal survival and ultimat... Read more »
USP7 COOPERATES WITH SCML2 TO REGULATE THE ACTIVITY OF PRC1. Published: January, 2015 Abstract:
USP7 is a protein deubiquitinase with an essential role in development. Here, we provide evidence that USP7 regulates the activity of the Polycomb Repressive Complex 1 (PRC1) in coordination with SCML2. There are six versions of PRC1 defined by th... Read more »
An AUTS2-Polycomb complex activates gene expression in the CNS. Published: December, 2014 Abstract:
Naturally occurring variations of Polycomb repressive complex 1 (PRC1) comprise a core assembly of Polycomb group proteins and additional factors that include, surprisingly, autism susceptibility candidate 2 (AUTS2). Although AUTS2 is often disrup... Read more »
RNA helicase DDX21 coordinates transcription and ribosomal RNA processing. Published: November, 2014 Abstract:
DEAD-box RNA helicases are vital for the regulation of various aspects of the RNA life cycle, but the molecular underpinnings of their involvement, particularly in mammalian cells, remain poorly understood. Here we show that the DEAD-box RNA helic... Read more »
DNA Copy-Number Control through Inhibition of Replication Fork Progression. Published: November, 2014 Abstract:
Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the lev... Read more »
AF9 YEATS Domain Links Histone Acetylation to DOT1L-Mediated H3K79 Methylation. Published: October, 2014 Abstract:
The recognition of modified histones by "reader" proteins constitutes a key mechanism regulating gene expression in the chromatin context. Compared with the great variety of readers for histone methylation, few protein modules that recognize histo... Read more »
ETS1 is a genome-wide effector of RAS/ERK signaling in epithelial cells. Published: October, 2014 Abstract:
The RAS/ERK pathway is commonly activated in carcinomas and promotes oncogenesis by altering transcriptional programs. However, the array of cis-regulatory elements and trans-acting factors that mediate these transcriptional changes is still uncle... Read more »
Genome-wide map of regulatory interactions in the human genome. Published: September, 2014 Abstract:
Increasing evidence suggests that interactions between regulatory genomic elements play an important role in regulating gene expression. We generated a genome-wide interaction map of regulatory elements in human cells (ENCODE tier 1 cells, K562, G... Read more »
Immunogenetics. Chromatin state dynamics during blood formation. Published: August, 2014 Abstract:
Chromatin modifications are crucial for development, yet little is known about their dynamics during differentiation. Hematopoiesis provides a well-defined model to study chromatin state dynamics; however, technical limitations impede profiling of... Read more »
FUS is sequestered in nuclear aggregates in ALS patient fibroblasts. Published: July, 2014 Abstract:
Mutations in the RNA-binding protein FUS have been shown to cause the neurodegenerative disease amyotrophic lateral sclerosis. We investigate whether mutant FUS protein in ALS patient-derived fibroblasts affects normal FUS functions in the nucleus... Read more »
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Published: April, 2014 Abstract:
Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided genome editing and transcription regulation in eukaryotic cells, yet their in vivo target specificity is poorly understood. Here we mapped genome-wide binding sites of a... Read more »
Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1. Published: April, 2014 Abstract:
Genetic deficiencies in transcription factors can lead to the loss of certain types of cells and tissue. The steroidogenic tissue-specific nuclear receptor Ad4BP/SF-1 (NR5A1) is one such gene, because mice in which this gene is disrupted fail to d... Read more »
ZBED6 Modulates the Transcription of Myogenic Genes in Mouse Myoblast Cells. Published: April, 2014 Abstract:
ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse m... Read more »
Identification of HilD-regulated genes in Salmonella enterica serovar Typhimurium. Published: March, 2014 Abstract:
Salmonella enterica serovar Typhimurium (S. Typhimurium) pathogenicity island 1 (SPI-1) encodes a type III secretion system required for invasion of host gut epithelial cells. Expression of SPI-1 virulence genes is controlled by a complex hierarch... Read more »
Epigenomic alterations define lethal CIMP-positive ependymomas of infancy. Published: February, 2014 Abstract:
Ependymomas are common childhood brain tumours that occur throughout the nervous system, but are most common in the paediatric hindbrain. Current standard therapy comprises surgery and radiation, but not cytotoxic chemotherapy as it does not furth... Read more »
Genome-wide binding patterns of thyroid hormone receptor beta. Published: February, 2014 Abstract:
Thyroid hormone (TH) receptors (TRs) play central roles in metabolism and are major targets for pharmaceutical intervention. Presently, however, there is limited information about genome wide localizations of TR binding sites. Thus, complexities o... Read more »
A cohesin-independent role for NIPBL at promoters provides insights in CdLS. Published: February, 2014 Abstract:
The cohesin complex is crucial for chromosome segregation during mitosis and has recently also been implicated in transcriptional regulation and chromatin architecture. The NIPBL protein is required for the loading of cohesin onto chromatin, but h... Read more »
CLOCK:BMAL1 is a pioneer-like transcription factor. Published: January, 2014 Abstract:
The mammalian circadian clock relies on the master genes CLOCK and BMAL1 to drive rhythmic gene expression and regulate biological functions under circadian control. Here we show that rhythmic CLOCK:BMAL1 DNA binding promotes rhythmic chromatin op... Read more »
Large conserved domains of low DNA methylation maintained by Dnmt3a. Published: January, 2014 Abstract:
Gains and losses in DNA methylation are prominent features of mammalian cell types. To gain insight into the mechanisms that promote shifts in DNA methylation and contribute to changes in cell fate, including malignant transformation, we performed... Read more »
Nfatc1 orchestrates aging in hair follicle stem cells. Published: December, 2013 Abstract:
Hair production is fueled by stem cells (SCs), which transition between cyclical bouts of rest and activity. Here, we explore why hair growth wanes with age. We show that aged hair follicle SCs (HFSCs) in mice exhibit enhanced resting and abbrevia... Read more »
Mediator links transcription and DNA repair by facilitating Rad2/XPG recruitment. Published: December, 2013 Abstract:
Mediator is a large multiprotein complex conserved in all eukaryotes. The crucial function of Mediator in transcription is now largely established. However, we found that this complex also plays an important role by connecting transcription with D... Read more »
Coupling transcription factor occupancy to nucleosome architecture with DNase-FLASH. Published: November, 2013 Abstract:
It is currently not possible to resolve the genome-wide relationship of transcription factors (TFs) and nucleosomes at the level of individual chromatin templates despite rapidly increasing data on TF and nucleosome occupancy in the human genome. ... Read more »
Promiscuous RNA binding by Polycomb repressive complex 2. Published: November, 2013 Abstract:
Polycomb repressive complex 2 (PRC2) is a histone methyltransferase required for epigenetic silencing during development and cancer. Long noncoding RNAs (lncRNAs) recruit PRC2 to chromatin, but the general role of RNA in maintaining repressed chro... Read more »
PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells. Published: November, 2013 Abstract:
EZH2 is the catalytic subunit of PRC2, a central epigenetic repressor essential for development processes in vivo and for the differentiation of embryonic stem cells (ESCs) in vitro. The biochemical function of PRC2 in depositing repressive H3K27m... Read more »
Gene dysregulation by histone variant H2A.Z in bladder cancer Published: October, 2013 Abstract:
Background The incorporation of histone variants into nucleosomes is one of the main strategies that the cell uses to regulate the structure and function of chromatin. Histone H2A.Z is an evolutionarily conserved histone H2A variant that is pref... Read more »
Genomic organization of human transcription initiation complexes. Published: October, 2013 Abstract:
The human genome is pervasively transcribed, yet only a small fraction is coding. Here we address whether this non-coding transcription arises at promoters, and detail the interactions of initiation factors TATA box binding protein (TBP), transcri... Read more »
Gene regulation and priming by topoisomerase IIα in embryonic stem cells. Published: September, 2013 Abstract:
Topoisomerases resolve torsional stress, while their function in gene regulation, especially during cellular differentiation, remains unknown. Here we find that the expression of topo II isoforms, topoisomerase IIα and topoisomerase IIβ, is the ch... Read more »
Addiction of t(8;21) and inv(16) acute myeloid leukemia to native RUNX1. Published: September, 2013 Abstract:
The t(8;21) and inv(16) chromosomal aberrations generate the oncoproteins AML1-ETO (A-E) and CBFβ-SMMHC (C-S). The role of these oncoproteins in acute myeloid leukemia (AML) etiology has been well studied. Conversely, the function of native RUNX1 ... Read more »
Ras-Induced Changes in H3K27me3 Occur after Those in Transcriptional Activity. Published: August, 2013 Abstract:
Oncogenic signaling pathways regulate gene expression in part through epigenetic modification of chromatin including DNA methylation and histone modification. Trimethylation of histone H3 at lysine-27 (H3K27), which correlates with transcriptional... Read more »
De Novo Mutations in the Genome Organizer CTCF Cause Intellectual Disability. Published: July, 2013 Abstract:
An increasing number of genes involved in chromatin structure and epigenetic regulation has been implicated in a variety of developmental disorders, often including intellectual disability. By trio exome sequencing and subsequent mutational screen... Read more »
Uniform, optimal signal processing of mapped deep-sequencing data. Published: July, 2013 Abstract:
Despite their apparent diversity, many problems in the analysis of high-throughput sequencing data are merely special cases of two general problems, signal detection and signal estimation. Here we adapt formally optimal solutions from signal proce... Read more »
Estrogen-dependent dynamic profile of eNOS-DNA associations in prostate cancer. Published: May, 2013 Abstract:
In previous work we have documented the nuclear translocation of endothelial NOS (eNOS) and its participation in combinatorial complexes with Estrogen Receptor Beta (ERβ) and Hypoxia Inducible Factors (HIFs) that determine localized chromatin remo... Read more »
Placing the HIRA histone chaperone complex in the chromatin landscape. Published: April, 2013 Abstract:
The HIRA chaperone complex, comprised of HIRA, UBN1, and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand HIRA's function and m... Read more »
Multiple roles for Piwi in silencing Drosophila transposons. Published: February, 2013 Abstract:
Silencing of transposons in the Drosophila ovary relies on three Piwi family proteins--Piwi, Aubergine (Aub), and Ago3--acting in concert with their small RNA guides, the Piwi-interacting RNAs (piRNAs). Aub and Ago3 are found in the germ cell cyto... Read more »
Specificity and Function of Archaeal DNA Replication Initiator Proteins. Published: January, 2013 Abstract:
Chromosomes with multiple DNA replication origins are a hallmark of Eukaryotes and some Archaea. All eukaryal nuclear replication origins are defined by the origin recognition complex (ORC) that recruits the replicative helicase MCM(2-7) via Cdc6 ... Read more »
MeCP2 modulates gene expression pathways in astrocytes. Published: January, 2013 Abstract:
ABSTRACT: BACKGROUND: Mutations in MECP2 encoding methyl-CpG-binding protein 2 (MeCP2) cause the X-linked neurodevelopmental disorder Rett syndrome. Rett syndrome patients exhibit neurological symptoms that include irregular breathing, impaired mo... Read more »
In Vivo Epigenomic Profiling of Germ Cells Reveals Germ Cell Molecular Signatures. Published: January, 2013 Abstract:
The limited number of in vivo germ cells poses an impediment to genome-wide studies. Here, we applied a small-scale chromatin immunoprecipitation sequencing (ChIP-seq) method on purified mouse fetal germ cells to generate genome-wide maps of four ... Read more »
Histone methyltransferase MLL3 contributes to genome-scale circadian transcription. Published: January, 2013 Abstract:
Daily cyclical expression of thousands of genes in tissues such as the liver is orchestrated by the molecular circadian clock, the disruption of which is implicated in metabolic disorders and cancer. Although we understand much about the circadian... Read more »
A Molecular Roadmap of Reprogramming Somatic Cells into iPS Cells. Published: December, 2012 Abstract:
Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is inefficient, complicating mechanistic studies. Here, we examined defined intermediate cell populations poised to becoming iPSCs by genome-wide analyses. W... Read more »
Limitations and possibilities of low cell number ChIP-seq. Published: November, 2012 Abstract:
ABSTRACT: BACKGROUND: Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting materi... Read more »
A genome-wide signature of glucocorticoid receptor binding in neuronal PC12 cells. Published: October, 2012 Abstract:
ABSTRACT: BACKGROUND: Glucocorticoids, secreted by the adrenals in response to stress, profoundly affect structure and plasticity of neurons. Glucocorticoid action in neurons is mediated by glucocorticoid receptors (GR) that operate as transcripti... Read more »
Widespread plasticity in CTCF occupancy linked to DNA methylation. Published: September, 2012 Abstract:
CTCF is a ubiquitously expressed regulator of fundamental genomic processes including transcription, intra- and interchromosomal interactions, and chromatin structure. Because of its critical role in genome function, CTCF binding patterns have lon... Read more »
Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA. Published: August, 2012 Abstract:
How different organs are formed from small sets of undifferentiated precursor cells is a key question in developmental biology. To understand the molecular mechanisms underlying organ specification in plants, we studied the function of the homeoti... Read more »
Cohesins Repress KSHV Immediate Early Gene Transcription During Latency. Published: June, 2012 Abstract:
Chromatin-organizing factors, like CTCF and cohesins, have been implicated in the control of complex viral regulatory programs. We investigated the role of CTCF and cohesin in the control of the latent to lytic switch for Kaposi's Sarcoma-Associat... Read more »
Functional Genomic Methods to Study Estrogen Receptor Activity. Published: May, 2012 Abstract:
Estrogen Receptor (ER) is a nuclear receptor that mediates the actions of estrogen and tamoxifen. ER is expressed in a major fraction of human breast cancers. Recently, genomic maps for estrogen- and tamoxifen-ER have been published. Interestingly... Read more »
The linker histone plays a dual role during gametogenesis in S. cerevisiae. Published: May, 2012 Abstract:
The differentiation of gametes involves dramatic changes to chromatin, affecting transcription, meiosis, and cell morphology. Sporulation in S. cerevisiae shares many chromatin features with spermatogenesis, including a ten-fold compaction of the ... Read more »
Genome Wide Analysis Of Pax8 Binding Provides New Insights Into Thyroid Functions. Published: April, 2012 Abstract:
ABSTRACT: BACKGROUND: The transcription factor Pax8 is essential for the differentiation of thyroid cells. However, there are few data on genes transcriptionally regulated by Pax8 other than thyroid-related genes. To better understand the role of ... Read more »
A dual role of linker histone H1.4 Lys 34 acetylation in transcriptional activation. Published: April, 2012 Abstract:
The linker histone H1 is a key player in chromatin organization, yet our understanding of the regulation of H1 functions by post-translational modifications is very limited. We provide here the first functional characterization of H1 acetylation. ... Read more »
Genome-wide analysis of histone H3.1 and H3.3 variants in Arabidopsis thaliana. Published: March, 2012 Abstract:
Nucleosomes package eukaryotic DNA and are composed of four different histone proteins, designated H3, H4, H2A, and H2B. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. We profiled H3... Read more »
Chromatin-modifying enzymes as modulators of reprogramming. Published: March, 2012 Abstract:
Generation of induced pluripotent stem cells (iPSCs) by somatic cell reprogramming involves global epigenetic remodelling. Whereas several proteins are known to regulate chromatin marks associated with the distinct epigenetic states of cells befor... Read more »
Widespread site-dependent buffering of human regulatory polymorphism. Published: March, 2012 Abstract:
The average individual is expected to harbor thousands of variants within non-coding genomic regions involved in gene regulation. However, it is currently not possible to interpret reliably the functional consequences of genetic variation within a... Read more »
Dynamic MicroRNA Gene Transcription and Processing during T Cell Development. Published: February, 2012 Abstract:
By disrupting microRNA (miRNA) biogenesis, we previously showed that this pathway is critical for the differentiation and function of T cells. Although various cloning studies have shown that many miRNAs are expressed during T cell development, an... Read more »
A workflow for genome-wide mapping of archaeal transcription factors with ChIP-seq. Published: February, 2012 Abstract:
Deciphering the structure of gene regulatory networks across the tree of life remains one of the major challenges in postgenomic biology. We present a novel ChIP-seq workflow for the archaea using the model organism Halobacterium salinarum sp. NRC... Read more »
Research Resource: The Pdx1 Cistrome of Pancreatic Islets. Published: February, 2012 Abstract:
The homeodomain transcription factor pancreas duodenal homeobox 1 (Pdx1, also known as insulin promoter factor 1) is a master regulator of pancreas development, as mice or humans lacking Pdx1 function are a pancreatic. Importantly, heterozygous mu... Read more »
The little elongation complex regulates small nuclear RNA transcription. Published: December, 2011 Abstract:
Eleven-nineteen lysine-rich leukemia (ELL) participates in the super elongation complex (SEC) with the RNA polymerase II (Pol II) CTD kinase P-TEFb. SEC is a key regulator in the expression of HOX genes in mixed lineage leukemia (MLL)-based hemato... Read more »
Distinct Epigenomic Features in End-Stage Failing Human Hearts Published: November, 2011 Abstract:
Background—The epigenome refers to marks on the genome, including DNA methylation and histone modifications, that regulate the expression of underlying genes. A consistent profile of gene expression changes in end-stage cardiomyopathy led us to hy... Read more »
Systematic bias in high-throughput sequencing data and its correction by BEADS. Published: August, 2011 Abstract:
Genomic sequences obtained through high-throughput sequencing are not uniformly distributed across the genome. For example, sequencing data of total genomic DNA show significant, yet unexpected enrichments on promoters and exons. This systematic b... Read more »
Occupancy of chromatin organizers in the Epstein-Barr virus genome. Published: June, 2011 Abstract:
The human CCCTC-binding factor, CTCF, regulates transcription of the double-stranded DNA genomes of herpesviruses. The architectural complex cohesin and RNA Polymerase II also contribute to this organization. We profiled the occupancy of CTCF, coh... Read more »
Mapping and analysis of chromatin state dynamics in nine human cell types. Published: May, 2011 Abstract:
Chromatin profiling has emerged as a powerful means of genome annotation and detection of regulatory activity. The approach is especially well suited to the characterization of non-coding portions of the genome, which critically contribute to cell... Read more »
Replication stress induces 53BP1-containing OPT domains in G1 cells. Published: April, 2011 Abstract:
Chromosomal deletions and rearrangements in tumors are often associated with common fragile sites, which are specific genomic loci prone to gaps and breaks in metaphase chromosomes. Common fragile sites appear to arise through incomplete DNA repli... Read more »
EpiChIP: gene-by-gene quantification of epigenetic modification levels. Published: March, 2011 Abstract:
The combination of chromatin immunoprecipitation with next-generation sequencing technology (ChIP-seq) is a powerful and increasingly popular method for mapping protein-DNA interactions in a genome-wide fashion. The conventional way of analyzing t... Read more »
ChIP-chip versus ChIP-seq: lessons for experimental design and data analysis. Published: January, 2011 Abstract:
BACKGROUND: Chromatin immunoprecipitation (ChIP) followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows genome-wide discovery of protein-DNA interactions such as transcription factor bindings and histone m... Read more »
Role of p53 serine 46 in p53 target gene regulation. Published: January, 2011 Abstract:
The tumor suppressor p53 plays a crucial role in cellular growth control inducing a plethora of different response pathways. The molecular mechanisms that discriminate between the distinct p53-responses have remained largely elusive. Here, we have... Read more »
ZNF274 recruits the histone methyltransferase SETDB1 to the 3' ends of ZNF genes. Published: December, 2010 Abstract:
Only a small percentage of human transcription factors (e.g. those associated with a specific differentiation program) are expressed in a given cell type. Thus, cell fate is mainly determined by cell type-specific silencing of transcription factor... Read more »
Mapping protein-DNA interactions using ChIP-sequencing. Abstract:
Chromatin immunoprecipitation (ChIP) allows enrichment of genomic regions which are associated with specific transcription factors, histone modifications, and indeed any other epitopes which are present on chromatin. The original ChIP methods used... Read more »
