D-Plex UDI for cell-free RNA-seq - Set A includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexing with the D-Plex cell-free RNA-seq Kit.
Two sets of indexes are available separately:
- C05030045 D-Plex UDI for cell-free RNA-seq - Set A
- C05030046 D-Plex UDI for cell-free RNA-seq - Set B
The use of UDIs is highly recommended to mitigate errors caused by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.
Each set can be used for library multiplexing of up to 24 samples. Sets A and B can be used simultaneously for library multiplexing of up to 48 samples.
The D-Plex technology utilizes capture and amplification by tailing and switching, a ligation-free method for library preparation, and offers key advantages such as:
- Low to ultra-low input capability
- Ease of use in a one-day, one-tube protocol
- High performance and library complexity
