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<p><small><strong>Figure 1. ChIP using the Diagenode monoclonal antibody against hERα </strong><br />from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 µg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006-050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.</small></p>
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<p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p>
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<p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p>
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<p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p>
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<p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p>
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<p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p>
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<p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p>
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<p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_wb.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 µg/ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_ic.png" alt="Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 µg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform.</small></p>
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<p><small><strong> Figure 1. ChIP using the Diagenode monoclonal antibody </strong><br /><span>ChIP using the Diagenode monoclonal antibody </span>against hERα from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting., the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 μg of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure 1. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene (Cat. No. pp-1006- 050), used as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig2.png" alt="ER alpha Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode monoclonal antibody against hERα </strong><br />Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the Diagenode monoclonal antibody directed against ER alpha (Cat. No. MAb-009-050) at a concentration of 7 μg/ ml. Figure 2 shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200009_fig3.png" alt="ER alpha Antibody validated in Immunocytochemistry" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 3. Immunocytochemistry using the Diagenode monoclonal antibody against hERα </strong><br />COS-7 cells transiently overexpressing human ERα (left) or ERß1 (right) were labeled with the Diagenode antibody against ER alpha (Cat. No. MAb-009-050), used at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure 3 shows the specificity of the antibody for the ER alpha isoform. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'id' => '5057',
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)
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(int) 0 => 'immunochemistry'
)
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'id' => '31',
'position' => '10',
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'name' => 'IC',
'description' => '<p>Immunochemistry</p>',
'in_footer' => false,
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'slug' => 'immunochemistry',
'meta_keywords' => 'Immunochemistry,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunochemistry applications',
'meta_title' => 'Immunochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:31',
'created' => '2015-07-08 13:48:10',
'locale' => 'zho'
)
$description = '<p>Immunochemistry</p>'
$name = 'IC'
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'id' => '505',
'name' => 'Datasheet hERalpha MAb-009-050',
'description' => 'Datasheet description',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/Datasheet_hERalpha_MAb-009-050.pdf',
'slug' => 'datasheet-heralpha-mab-009-050',
'meta_keywords' => null,
'meta_description' => null,
'modified' => '2015-07-07 11:47:44',
'created' => '2015-07-07 11:47:44',
'ProductsDocument' => array(
'id' => '283',
'product_id' => '1969',
'document_id' => '505'
)
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'id' => '572',
'name' => 'ER alpha antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/ER/SDS-C15200009-ER_alpha_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 14:57:14',
'created' => '2020-07-01 14:57:14',
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'id' => '1103',
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'safety_sheet_id' => '572'
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$publication = array(
'id' => '3363',
'name' => 'Oestrogen Receptor-α binds the FOXP3 promoter and modulates regulatory T-cell function in human cervical cancer',
'authors' => 'Adurthi S. et al.',
'description' => '<p>Oestrogen controls Foxp3 expression in regulatory T cells (T<sub>reg</sub> cells) via a mechanism thought to involve oestrogen receptor alpha (ERα), but the molecular basis and functional impact of ERα signalling in T<sub>reg</sub> cells remain unclear. We report that ERα ligand oestradiol (E2) is significantly increased in human cervical cancer (CxCa) tissues and tumour-infiltrating T<sub>reg</sub> cells (CD4<sup>+</sup>CD25<sup>hi</sup>CD127<sup>low</sup>), whereas blocking ERα with the antagonist ICI 182,780 abolishes FOXP3 expression and impairs the function of CxCa infiltrating T<sub>reg</sub> cells. Using a novel approach of co-immunoprecipitation with antibodies to E2 for capture, we identified binding of E2:ERα complexes to FOXP3 protein in CxCa-derived T<sub>reg</sub> cells. Chromatin immunoprecipitation analyses of male blood T<sub>reg</sub> cells revealed ERα occupancy at the FOXP3 promoter and conserved non-coding DNA elements 2 and 3. Accordingly, computational analyses of the enriched regions uncovered eight putative oestrogen response elements predicted to form a loop that can activate the FOXP3 promoter. Together, these data suggest that E2-mediated ERα signalling is critical for the sustenance of FOXP3 expression and T<sub>reg</sub> cell function in human CxCa via direct interaction of ERα with FOXP3 promoter. Overall, our work gives a molecular insight into ERα signalling and highlights a fundamental role of E2 in controlling human T<sub>reg</sub> cell physiology.</p>',
'date' => '2017-12-11',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/29229929',
'doi' => '',
'modified' => '2018-04-24 10:07:53',
'created' => '2018-04-24 10:07:53',
'ProductsPublication' => array(
'id' => '2735',
'product_id' => '1969',
'publication_id' => '3363'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/29229929" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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