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<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<td>2 μg/ChIP</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:5,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>WB</td>
<td>1:500</td>
<td>Fig 4</td>
</tr>
</tbody>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p>
<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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