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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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