Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'product' => array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode'
$meta_description = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available'
$meta_title = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode'
$product = array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '208',
'name' => 'C15200233',
'product_id' => '2962',
'modified' => '2018-03-01 15:10:47',
'created' => '2017-04-26 17:11:35'
),
'Master' => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '123',
'position' => '4',
'parent_id' => '100',
'name' => 'CRISPR/Cpf1 antibodies',
'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p>
<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
</ul>
<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
<div class="small-9 medium-9 large-9 columns">
<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
</div>
</div>
<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
</ul>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div>
<div class="small-7 medium-7 large-7 columns">
<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'crispr-cpf1-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cpf1 polyclonal antibody,CRISPR/Cas9 Antibodies,diagenode',
'meta_description' => 'Diagenode offer the first antibodies Raised against the Cpf1 nuclease from Acidaminococcus sp., described as a potential candidate for genome editing in mammalian cells.',
'meta_title' => 'CRISPR/Cpf1 polyclonal antibodies | Diagenode',
'modified' => '2018-03-29 11:58:50',
'created' => '2016-09-09 11:04:51',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
)
),
'Document' => array(
(int) 0 => array(
'id' => '948',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/l-bacterium-crispr-cpf1-monoclonal-antibody.pdf',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:57:56',
'created' => '2017-04-26 16:57:56',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1126',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-GB-en-GHS_3_0.pdf',
'countries' => 'GB',
'modified' => '2021-01-26 13:14:24',
'created' => '2021-01-26 13:14:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1128',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-US-en-GHS_3_0.pdf',
'countries' => 'US',
'modified' => '2021-01-26 13:15:41',
'created' => '2021-01-26 13:15:41',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1123',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-DE-de-GHS_3_0.pdf',
'countries' => 'DE',
'modified' => '2021-01-26 13:12:35',
'created' => '2021-01-26 13:12:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1127',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-01-26 13:15:04',
'created' => '2021-01-26 13:15:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1122',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-nl-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:12:07',
'created' => '2021-01-26 13:12:07',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1121',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-fr-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:11:29',
'created' => '2021-01-26 13:11:29',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1125',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-FR-fr-GHS_3_0.pdf',
'countries' => 'FR',
'modified' => '2021-01-26 13:13:34',
'created' => '2021-01-26 13:13:34',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
(int) 1 => array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
)
$pro = array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'ProductsGroup' => array(
'id' => '279',
'product_id' => '2887',
'group_id' => '208'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
'id' => '4631',
'product_id' => '2887',
'application_id' => '19'
)
)
$slugs = array(
(int) 0 => 'western-blot-antibodies'
)
$applications = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2542',
'product_id' => '2887',
'document_id' => '991'
)
)
$sds = array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
'id' => '1969',
'product_id' => '2887',
'safety_sheet_id' => '1124'
)
)
$publication = array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
'id' => '3762',
'product_id' => '2887',
'publication_id' => '3683'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30804551" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'product' => array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode'
$meta_description = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available'
$meta_title = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode'
$product = array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '208',
'name' => 'C15200233',
'product_id' => '2962',
'modified' => '2018-03-01 15:10:47',
'created' => '2017-04-26 17:11:35'
),
'Master' => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '123',
'position' => '4',
'parent_id' => '100',
'name' => 'CRISPR/Cpf1 antibodies',
'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p>
<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
</ul>
<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
<div class="small-9 medium-9 large-9 columns">
<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
</div>
</div>
<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
</ul>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div>
<div class="small-7 medium-7 large-7 columns">
<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'crispr-cpf1-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cpf1 polyclonal antibody,CRISPR/Cas9 Antibodies,diagenode',
'meta_description' => 'Diagenode offer the first antibodies Raised against the Cpf1 nuclease from Acidaminococcus sp., described as a potential candidate for genome editing in mammalian cells.',
'meta_title' => 'CRISPR/Cpf1 polyclonal antibodies | Diagenode',
'modified' => '2018-03-29 11:58:50',
'created' => '2016-09-09 11:04:51',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
)
),
'Document' => array(
(int) 0 => array(
'id' => '948',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/l-bacterium-crispr-cpf1-monoclonal-antibody.pdf',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:57:56',
'created' => '2017-04-26 16:57:56',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1126',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-GB-en-GHS_3_0.pdf',
'countries' => 'GB',
'modified' => '2021-01-26 13:14:24',
'created' => '2021-01-26 13:14:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1128',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-US-en-GHS_3_0.pdf',
'countries' => 'US',
'modified' => '2021-01-26 13:15:41',
'created' => '2021-01-26 13:15:41',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1123',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-DE-de-GHS_3_0.pdf',
'countries' => 'DE',
'modified' => '2021-01-26 13:12:35',
'created' => '2021-01-26 13:12:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1127',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-01-26 13:15:04',
'created' => '2021-01-26 13:15:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1122',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-nl-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:12:07',
'created' => '2021-01-26 13:12:07',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1121',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-fr-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:11:29',
'created' => '2021-01-26 13:11:29',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1125',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-FR-fr-GHS_3_0.pdf',
'countries' => 'FR',
'modified' => '2021-01-26 13:13:34',
'created' => '2021-01-26 13:13:34',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
(int) 1 => array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
)
$pro = array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'ProductsGroup' => array(
'id' => '279',
'product_id' => '2887',
'group_id' => '208'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
'id' => '4631',
'product_id' => '2887',
'application_id' => '19'
)
)
$slugs = array(
(int) 0 => 'western-blot-antibodies'
)
$applications = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2542',
'product_id' => '2887',
'document_id' => '991'
)
)
$sds = array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
'id' => '1969',
'product_id' => '2887',
'safety_sheet_id' => '1124'
)
)
$publication = array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
'id' => '3762',
'product_id' => '2887',
'publication_id' => '3683'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30804551" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'product' => array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode'
$meta_description = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available'
$meta_title = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode'
$product = array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '208',
'name' => 'C15200233',
'product_id' => '2962',
'modified' => '2018-03-01 15:10:47',
'created' => '2017-04-26 17:11:35'
),
'Master' => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '123',
'position' => '4',
'parent_id' => '100',
'name' => 'CRISPR/Cpf1 antibodies',
'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p>
<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
</ul>
<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
<div class="small-9 medium-9 large-9 columns">
<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
</div>
</div>
<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
</ul>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div>
<div class="small-7 medium-7 large-7 columns">
<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'crispr-cpf1-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cpf1 polyclonal antibody,CRISPR/Cas9 Antibodies,diagenode',
'meta_description' => 'Diagenode offer the first antibodies Raised against the Cpf1 nuclease from Acidaminococcus sp., described as a potential candidate for genome editing in mammalian cells.',
'meta_title' => 'CRISPR/Cpf1 polyclonal antibodies | Diagenode',
'modified' => '2018-03-29 11:58:50',
'created' => '2016-09-09 11:04:51',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
)
),
'Document' => array(
(int) 0 => array(
'id' => '948',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/l-bacterium-crispr-cpf1-monoclonal-antibody.pdf',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:57:56',
'created' => '2017-04-26 16:57:56',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1126',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-GB-en-GHS_3_0.pdf',
'countries' => 'GB',
'modified' => '2021-01-26 13:14:24',
'created' => '2021-01-26 13:14:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1128',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-US-en-GHS_3_0.pdf',
'countries' => 'US',
'modified' => '2021-01-26 13:15:41',
'created' => '2021-01-26 13:15:41',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1123',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-DE-de-GHS_3_0.pdf',
'countries' => 'DE',
'modified' => '2021-01-26 13:12:35',
'created' => '2021-01-26 13:12:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1127',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-01-26 13:15:04',
'created' => '2021-01-26 13:15:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1122',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-nl-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:12:07',
'created' => '2021-01-26 13:12:07',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1121',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-fr-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:11:29',
'created' => '2021-01-26 13:11:29',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1125',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-FR-fr-GHS_3_0.pdf',
'countries' => 'FR',
'modified' => '2021-01-26 13:13:34',
'created' => '2021-01-26 13:13:34',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
(int) 1 => array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
)
$pro = array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'ProductsGroup' => array(
'id' => '279',
'product_id' => '2887',
'group_id' => '208'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
'id' => '4631',
'product_id' => '2887',
'application_id' => '19'
)
)
$slugs = array(
(int) 0 => 'western-blot-antibodies'
)
$applications = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2542',
'product_id' => '2887',
'document_id' => '991'
)
)
$sds = array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
'id' => '1969',
'product_id' => '2887',
'safety_sheet_id' => '1124'
)
)
$publication = array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
'id' => '3762',
'product_id' => '2887',
'publication_id' => '3683'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30804551" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'carrousel_order' => array(
(int) 0 => 'Gene editing'
),
'language' => 'cn',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'product' => array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
)
$carrousel_order = array(
(int) 0 => 'Gene editing'
)
$language = 'cn'
$meta_keywords = 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode'
$meta_description = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available'
$meta_title = 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode'
$product = array(
'Product' => array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => '505',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in Acidaminococcus sp. and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
'clonality' => '',
'isotype' => '',
'lot' => '001',
'concentration' => '1 µg/µl',
'reactivity' => 'Lachnospiraceae bacterium',
'type' => 'Monoclonal',
'purity' => 'Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.',
'classification' => '',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1:20,000</td>
<td>Fig 1, 2</td>
</tr>
</tbody>
</table>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => '',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:01:16',
'created' => '2017-04-26 15:39:07',
'select_label' => '505 - L. bacterium CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - Lachnospiraceae bacterium - Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '208',
'name' => 'C15200233',
'product_id' => '2962',
'modified' => '2018-03-01 15:10:47',
'created' => '2017-04-26 17:11:35'
),
'Master' => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '123',
'position' => '4',
'parent_id' => '100',
'name' => 'CRISPR/Cpf1 antibodies',
'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p>
<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
</ul>
<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
<div class="small-9 medium-9 large-9 columns">
<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
</div>
</div>
<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
</ul>
<div class="row">
<div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div>
<div class="small-7 medium-7 large-7 columns">
<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'crispr-cpf1-antibodies',
'cookies_tag_id' => '1',
'meta_keywords' => 'CRISPR/Cpf1 polyclonal antibody,CRISPR/Cas9 Antibodies,diagenode',
'meta_description' => 'Diagenode offer the first antibodies Raised against the Cpf1 nuclease from Acidaminococcus sp., described as a potential candidate for genome editing in mammalian cells.',
'meta_title' => 'CRISPR/Cpf1 polyclonal antibodies | Diagenode',
'modified' => '2018-03-29 11:58:50',
'created' => '2016-09-09 11:04:51',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array(
[maximum depth reached]
)
)
),
'Document' => array(
(int) 0 => array(
'id' => '948',
'name' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody',
'description' => '<p>Monoclonal antibody raised in mouse against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/l-bacterium-crispr-cpf1-monoclonal-antibody.pdf',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-26 16:57:56',
'created' => '2017-04-26 16:57:56',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1782',
'name' => 'product/antibodies/cas9-icon.png',
'alt' => 'CRISPR/Cas9 Antibody ',
'modified' => '2020-11-27 07:01:20',
'created' => '2018-03-15 15:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1126',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-GB-en-GHS_3_0.pdf',
'countries' => 'GB',
'modified' => '2021-01-26 13:14:24',
'created' => '2021-01-26 13:14:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1128',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-US-en-GHS_3_0.pdf',
'countries' => 'US',
'modified' => '2021-01-26 13:15:41',
'created' => '2021-01-26 13:15:41',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1123',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-DE-de-GHS_3_0.pdf',
'countries' => 'DE',
'modified' => '2021-01-26 13:12:35',
'created' => '2021-01-26 13:12:35',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1127',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-01-26 13:15:04',
'created' => '2021-01-26 13:15:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1122',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-nl-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:12:07',
'created' => '2021-01-26 13:12:07',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1121',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-BE-fr-GHS_3_0.pdf',
'countries' => 'BE',
'modified' => '2021-01-26 13:11:29',
'created' => '2021-01-26 13:11:29',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1125',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-FR-fr-GHS_3_0.pdf',
'countries' => 'FR',
'modified' => '2021-01-26 13:13:34',
'created' => '2021-01-26 13:13:34',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/cn/p/l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2962',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <em>Lachnospiraceae bacterium</em> (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-1.jpg" alt="WB" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. </small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15200233-wb-2-bis.jpg" alt="WB" /></div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1</strong><br /> Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag. </small></p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '100 µg',
'catalog_number' => 'C15200233-100',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000526',
'type' => 'FRE',
'search_order' => '03-Antibody',
'price_EUR' => '555',
'price_USD' => '580',
'price_GBP' => '500',
'price_JPY' => '86940',
'price_CNY' => '0',
'price_AUD' => '1450',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-100-ug',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:27:54',
'created' => '2018-03-01 15:10:18'
),
(int) 1 => array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
)
$pro = array(
'id' => '2887',
'antibody_id' => '505',
'name' => '<em>L. bacterium</em> CRISPR/Cpf1 Antibody',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 µg/50 µl',
'catalog_number' => 'C15200233-50',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '445',
'price_USD' => '465',
'price_GBP' => '420',
'price_JPY' => '69710',
'price_CNY' => '0',
'price_AUD' => '1162',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-50',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => 'L. bacterium CRISPR/Cpf1 monoclonal antibody,Western blot,CRISPR,Diagenode',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-01-05 15:28:18',
'created' => '2017-04-26 15:59:17',
'ProductsGroup' => array(
'id' => '279',
'product_id' => '2887',
'group_id' => '208'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2888',
'antibody_id' => '505',
'name' => '<i>L. bacterium</i> CRISPR/Cpf1 Antibody (sample size)',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µg',
'catalog_number' => 'C15200233-10',
'old_catalog_number' => '',
'sf_code' => 'C15200233-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '125',
'price_USD' => '130',
'price_GBP' => '120',
'price_JPY' => '19580',
'price_CNY' => '0',
'price_AUD' => '325',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => 'April 25, 2017',
'slug' => 'l-bacterium-crispr-cpf1-monoclonal-antibody-10',
'meta_title' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody | Diagenode',
'meta_keywords' => '',
'meta_description' => 'L. bacterium CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.',
'modified' => '2022-01-05 15:28:06',
'created' => '2017-04-26 17:10:09',
'ProductsGroup' => array(
'id' => '233',
'product_id' => '2888',
'group_id' => '208'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
'id' => '4631',
'product_id' => '2887',
'application_id' => '19'
)
)
$slugs = array(
(int) 0 => 'western-blot-antibodies'
)
$applications = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '991',
'name' => ' Accurate QC to optimize CRISPR/Cas9 genome editing specificity',
'description' => '<p>The CRISPR/Cas9 technology is delivering superior genetic models for fundamental disease research, drug screening, therapy development, rapid diagnostics, and transcriptional modulation. Although CRISPR/Cas9 enables rapid genome editing, several aspects affect its efficiency and specificity including guide RNA design, delivery methods, and off-targets effects. Diagenode has developed strategies to overcome these common pitfalls and has optimized CRISPR/Cas9 genome editing specificity</p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/CRISPR-Cas9-Poster-Accurate_QC.pdf',
'slug' => 'crispr-cas9-accurate-qc',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-02-12 15:36:31',
'created' => '2018-02-12 13:15:37',
'ProductsDocument' => array(
'id' => '2542',
'product_id' => '2887',
'document_id' => '991'
)
)
$sds = array(
'id' => '1124',
'name' => 'L.bacterium CRISPR Cpf1 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/L_bacterium/SDS-C15200233-L_bacterium_CRISPR_Cpf1_Antibody-ES-es-GHS_3_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 13:13:06',
'created' => '2021-01-26 13:13:06',
'ProductsSafetySheet' => array(
'id' => '1969',
'product_id' => '2887',
'safety_sheet_id' => '1124'
)
)
$publication = array(
'id' => '3683',
'name' => 'One-step generation of modular CAR-T cells with AAV-Cpf1.',
'authors' => 'Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S',
'description' => '<p>Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activating CRISPR (clustered regularly interspaced short palindromic repeats) RNA (tracrRNA)-independent CRISPR-Cpf1 systems with adeno-associated virus (AAV), we were able to build a stable CAR-T cell with homology-directed-repair knock-in and immune-checkpoint knockout (KIKO CAR-T cell) at high efficiency in one step. The modularity of the AAV-Cpf1 KIKO system enables flexible and highly efficient generation of double knock-in of two different CARs in the same T cell. Compared with Cas9-based methods, the AAV-Cpf1 system generates double-knock-in CAR-T cells more efficiently. CD22-specific AAV-Cpf1 KIKO CAR-T cells have potency comparable to that of Cas9 CAR-T cells in cytokine production and cancer cell killing, while expressing lower levels of exhaustion markers. This versatile system opens new capabilities of T-cell engineering with simplicity and precision.</p>',
'date' => '2019-03-01',
'pmid' => 'http://www.pubmed.gov/30804551',
'doi' => '10.1038/s41592-019-0329-7',
'modified' => '2019-07-01 11:15:29',
'created' => '2019-06-21 14:55:31',
'ProductsPublication' => array(
'id' => '3762',
'product_id' => '2887',
'publication_id' => '3683'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30804551" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
