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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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'description' => '<p>Diagenode is pleased to offer the <strong>first antibodies</strong> directed against the <strong>CRISPR/Cas9</strong> nuclease from <strong><em>Staphylococcus aureus</em></strong>.</p>
<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
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<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>N-terminus</strong> of Cas9 nuclease</li>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
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<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
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<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
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<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
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<th>Specificity</th>
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<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-WB.jpg" alt="WB figure 1" /></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
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<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
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<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
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<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
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<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
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<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
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<th>Antibody</th>
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<th>Specificity</th>
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<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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'pmid' => 'https://www.nature.com/articles/s41467-017-01222-y',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
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<p><small> <strong>Western blot analysis using the Diagenode monoclonal antibody directed against <em>S. aureus</em> Cas9</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with <em>S. aureus</em> Cas 9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15200230), diluited 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
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<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
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<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
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<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
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<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
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<th>Antibody</th>
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<th>IF</th>
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<th>Specificity</th>
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<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
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<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310260). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (N-terminal)</strong> <br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<h3><em>S. aureus</em> Cas9 monoclonal antibody</h3>
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<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crispr-cas9-monoclonal-antibody" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310260-IF.jpg" alt="IF figure 1" /></p>
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<p><small> <strong>Immunofluorescence using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (N-terminal)</strong><br />Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the <em>S. aureus</em> CRISPR/Cas9 antibody (cat. No. C15310260) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size" class="tiny details button radius">Learn more</a></div>
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<h3><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</h3>
<ul>
<li>Validated in <strong>WB</strong>, <strong>IF</strong> and <strong>IP</strong></li>
<li>Raised against <strong>C-terminus</strong> of Cas9 nuclease</li>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310259-IP.jpg" alt="IP figure 1" width="400" height="343" /></p>
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<div class="small-8 columns">
<p><small> <strong>IP using the Diagenode antibody directed against <em>S. aureus</em> CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 µg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 µl of the Diagenode antibody against CRISPR/Cas9 (cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 µg) is shown in lane 1 and 2.</small></p>
<div class="small-12 medium-12 large-12 columns text-right"><a href="../p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal" class="tiny details button radius">Learn more</a></div>
</div>
</div>
<h3>Which CRISPR/Cas9 antibody is the best for your application?</h3>
<table>
<thead>
<tr>
<th>Antibody</th>
<th>WB</th>
<th>IP</th>
<th>IF</th>
<th>Antibody raised against</th>
<th>Specificity</th>
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<tbody>
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<td><a href="/p/s-aureus-crispr-cas9-monoclonal-antibody"><em>S. aureus</em> CRISPR/Cas9 monoclonal antibody</a></td>
<td>+++</td>
<td>+++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-n-terminal-sample-size"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (N-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>N-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
</tr>
<tr>
<td><a href="/p/s-aureus-crisprcas9-polyclonal-antibody-c-terminal"><em>S. aureus</em> CRISPR/Cas9 polyclonal antibody (C-terminal)</a></td>
<td>++</td>
<td>++</td>
<td>+++</td>
<td>C-terminus of Cas9 nuclease</td>
<td><em>Staphylococcus aureus</em></td>
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<p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with S. aureus Cas9 (lane 2) and HeLa cells transfected with S. pyogenes Cas9 (lane 3) using the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259), diluited 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong><br />IP was performed on whole cell extracts (200 μg) from HEK293 cells transfected with a Cas9 expression vector (lane 1 and 3), or untransfected cells (lane 2 and 4) using 1 μl of the Diagenode antibody against CRISPR/Cas9 (Cat. No. C15310259). The immunoprecipitated proteins were subsequently analysed by Western blot with the monoclonal CRISPR/Cas9 antibody (C15200230). Lane 3 and 4 show the result of the IP, the input (10 μg) is shown in lane 1 and 2.</small></p>
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<p><small> <strong>Figure 3. Immunofluorescence using the Diagenode antibody directed against S. aureus CRISPR/Cas9 (C-terminal)</strong> <br /> Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the S. aureus CRISPR/ Cas9 antibody (Cat. No. C15310259) diluted 1:1,000 in blocking solution at 4°C o/n, followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-GFP antibody.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>'
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