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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against SKI8 </strong><br />Western blot was performed on nuclear extract from HeLa cells (lane 1), Drosophila adult whole cell extracts (lane 2), and the purified human PAF complex (lane 3) (Figure 1A) or on nuclear extracts from HeLa cells (HeLa NE, 20 μg) (Figure 1B), using the Diagenode antibody against SKI8 (Cat. No. pAb-012-150) diluted 1:1,000. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against SKI8 </strong><br />Western blot was performed on nuclear extract from HeLa cells (lane 1), Drosophila adult whole cell extracts (lane 2), and the purified human PAF complex (lane 3) (Figure 1A) or on nuclear extracts from HeLa cells (HeLa NE, 20 μg) (Figure 1B), using the Diagenode antibody against SKI8 (Cat. No. pAb-012-150) diluted 1:1,000. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'url' => 'files/SDS/Ski8/SDS-C15410012-Ski8_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
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'created' => '2021-01-26 10:16:04',
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[maximum depth reached]
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),
(int) 6 => array(
'id' => '989',
'name' => 'Ski8 antibody SDS FR fr',
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'countries' => 'FR',
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),
(int) 7 => array(
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'name' => 'Ski8 antibody SDS ES es',
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'created' => '2021-01-26 10:17:20',
'ProductsSafetySheet' => array(
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)
)
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$country = 'US'
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(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-012-150)</span></small>'
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '4313',
'product_id' => '2179',
'application_id' => '43'
)
)
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(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '1754',
'product_id' => '2179',
'document_id' => '38'
)
)
$sds = array(
'id' => '988',
'name' => 'Ski8 antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Ski8/SDS-C15410012-Ski8_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-01-26 10:17:20',
'created' => '2021-01-26 10:17:20',
'ProductsSafetySheet' => array(
'id' => '1731',
'product_id' => '2179',
'safety_sheet_id' => '988'
)
)
$publication = array(
'id' => '3065',
'name' => 'Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a',
'authors' => 'Strikoudis A. et al.',
'description' => '<p>Pluripotent embryonic stem cells (ESCs) self-renew or differentiate into all tissues of the developing embryo and cell-specification factors are necessary to balance gene expression. Here we delineate the function of the PHD-finger protein 5a (Phf5a) in ESC self-renewal and ascribe its role in regulating pluripotency, cellular reprogramming and myoblast specification. We demonstrate that Phf5a is essential for maintaining pluripotency, since depleted ESCs exhibit hallmarks of differentiation. Mechanistically, we attribute Phf5a function to the stabilization of the Paf1 transcriptional complex and control of RNA polymerase II elongation on pluripotency loci. Apart from an ESC-specific factor, we demonstrate that Phf5a controls differentiation of adult myoblasts. Our findings suggest a potent mode of regulation by Phf5a in stem cells, which directs their transcriptional programme, ultimately regulating maintenance of pluripotency and cellular reprogramming.</p>',
'date' => '2016-10-17',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27749823',
'doi' => '',
'modified' => '2016-11-08 09:38:01',
'created' => '2016-11-08 09:38:01',
'ProductsPublication' => array(
'id' => '1693',
'product_id' => '2179',
'publication_id' => '3065'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/27749823" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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