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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human <strong>SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1),</strong> using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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