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TBP Antibody - ChIP-seq Grade (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

目录号

格式

价格

C15200002-10

10 µg

$115.00
Bulk order

其他格式

C15200002

100 µg

Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID

Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).

Lot	DA-0010
Concentration
Species reactivity	Human, mouse
Type	Monoclonal ChIP grade, ChIP-seq grade
Purity	Ammonium sulphate purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	4 - 5 μg/IP	Fig 1, 2
WB	1:500	Fig 3

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.

Validation Data

Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP
ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.

A.
B.
C.
D.

Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP
ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.

Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP
Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Target Description
Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).

应用

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

ChIP-seq (ab)
Read more

ChIP-qPCR (ab)
Read more

siRNA Knockdown
Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level o... Read more

出版物

How to properly cite this product in your work

Diagenode strongly recommends using this: TBP Antibody - ChIP-seq Grade (sample size) (Diagenode Cat# C15200002-10 Lot# DA-0010). Click here to copy to clipboard.

Using our products in your publication? Let us know!

Enhanced frequency of transcription pre-initiation complexes assemblyafter exposure to UV irradiation results in increased repair activity andreduced probabilities for mutagenesis.
Liakos A. et al.
In addition to being essential for gene expression, transcription is crucial for the maintenance of genome integrity. Here, we undertook a systematic approach, to monitor the assembly kinetics of the pre-initiating RNA Polymerase (Pol) II at promoters at steady state and different stages during recovery from UV irra...

A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.
Llimos G. et al.
Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits....

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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<p>In addition to being essential for gene expression, transcription is crucial for the maintenance of genome integrity. Here, we undertook a systematic approach, to monitor the assembly kinetics of the pre-initiating RNA Polymerase (Pol) II at promoters at steady state and different stages during recovery from UV irradiation-stress, when pre-initiation and initiation steps have been suggested to be transiently shut down. Taking advantage of the reversible dissociation of pre-initiating Pol II after high salt treatment, we found that de novo recruitment of the available Pol II molecules at active promoters not only persists upon UV at all times tested but occurs significantly faster in the early phase of recovery (2 h) than in unexposed human fibroblasts at the majority of active genes. Our method unveiled groups of genes with significantly different pre-initiation complex (PIC) assembly dynamics after UV that present distinct rates of UV-related mutational signatures in melanoma tumours, providing functional relevance to the importance of keeping transcription initiation active during UV recovery. Our findings uncover novel mechanistic insights further detailing the multilayered transcriptional response to genotoxic stress and link PIC assembly dynamics after exposure to genotoxins with cancer mutational landscapes.</p>',
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			'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning &gt;100&thinsp;kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning &gt;150&thinsp;kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>',
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		'antibody_id' => '313',
		'name' => 'TBP Antibody',
		'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p>
<p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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	'antibody_id' => '313',
	'name' => 'TBP Antibody',
	'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p>
<p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<div class="row">
<div class="small-3 columns">
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>'
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	'date' => '2022-04-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565',
	'doi' => '10.1038/s41467-022-29625-6',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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		'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p>
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<div class="row">
<div class="small-3 columns">
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<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
			'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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			'date' => '2023-07-01',
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			'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning &gt;100&thinsp;kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning &gt;150&thinsp;kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>',
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		'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p>
<p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<div class="small-8 columns">
<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<div class="small-3 columns">
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<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<h3>Epigenetic antibodies you can trust!</h3>
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<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
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<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>',
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		'format' => '10 µg',
		'catalog_number' => 'C15200002-10',
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		'sf_code' => 'C15200002-D001-000582',
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		'slug' => 'tbp-monoclonal-antibody-classic-10',
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		'modified' => '2022-01-05 14:58:18',
		'created' => '2020-10-29 11:12:44',
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		'host' => '*****',
		'id' => '585',
		'name' => 'TBP Antibody - ChIP-seq Grade (sample size)',
		'description' => '',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'DA-0010',
		'concentration' => '',
		'reactivity' => 'Human, mouse',
		'type' => 'Monoclonal <strong>ChIP grade, ChIP-seq grade</strong>',
		'purity' => 'Ammonium sulphate purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 - 5 &mu;g/IP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>WB</td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;l per IP.</small></p>',
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'tbp-monoclonal-antibody-classic-10',
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		'modified' => '2020-11-04 15:39:37',
		'created' => '2020-10-29 11:08:01',
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			'name' => 'C15200002 TBP Antibody',
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			'created' => '2020-10-29 11:13:43'
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			'id' => '1960',
			'antibody_id' => '313',
			'name' => 'TBP Antibody',
			'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p>
<p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div>
</div>
<div class="spacer">
<p></p>
</div>
<div class="spacer">
<p></p>
</div>
<div class="spacer">
<p></p>
</div>
<div class="spacer">
<p></p>
</div>
<div class="spacer">
<p></p>
</div>
<div class="spacer">
<p></p>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
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			'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated  in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
			'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
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			'description' => '<div class="row">
<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
</div>
<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
			'meta_title' => 'Transcription factor Antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'ChIP-grade antibodies',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
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<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>'
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	'doi' => '10.1038/s41467-022-29625-6',
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		'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p>
<p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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			'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated  in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<li>100% satisfaction guarantee</li>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<p>In addition to being essential for gene expression, transcription is crucial for the maintenance of genome integrity. Here, we undertook a systematic approach, to monitor the assembly kinetics of the pre-initiating RNA Polymerase (Pol) II at promoters at steady state and different stages during recovery from UV irradiation-stress, when pre-initiation and initiation steps have been suggested to be transiently shut down. Taking advantage of the reversible dissociation of pre-initiating Pol II after high salt treatment, we found that de novo recruitment of the available Pol II molecules at active promoters not only persists upon UV at all times tested but occurs significantly faster in the early phase of recovery (2 h) than in unexposed human fibroblasts at the majority of active genes. Our method unveiled groups of genes with significantly different pre-initiation complex (PIC) assembly dynamics after UV that present distinct rates of UV-related mutational signatures in melanoma tumours, providing functional relevance to the importance of keeping transcription initiation active during UV recovery. Our findings uncover novel mechanistic insights further detailing the multilayered transcriptional response to genotoxic stress and link PIC assembly dynamics after exposure to genotoxins with cancer mutational landscapes.</p>',
			'date' => '2023-07-01',
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			'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning &gt;100&thinsp;kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning &gt;150&thinsp;kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>',
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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	'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p>
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 &mu;g of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the &ldquo;Auto Histone ChIP-seq&rdquo; kit (Cat. No. C01010022) on the IP-Star automated system. The IP&rsquo;d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP&rsquo;d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div>
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<p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 &mu;g of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 &mu;g) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
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<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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	'date' => '2022-04-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565',
	'doi' => '10.1038/s41467-022-29625-6',
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Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Datasheet TBP C15200002 DATASHEET Datasheet description	Download