Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).
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Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID
Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).
| Lot | DA-0010 |
|---|---|
| Concentration | |
| Species reactivity | Human, mouse |
| Type | Monoclonal ChIP grade, ChIP-seq grade |
| Purity | Ammonium sulphate purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 4 - 5 μg/IP | Fig 1, 2 |
| WB | 1:500 | Fig 3 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.
Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP
ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.
Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP
ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.
Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP
Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| siRNA Knockdown Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level o... Read more |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Datasheet TBP C15200002 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: TBP Antibody (sample size) (Diagenode Cat# C15200002-10 Lot# DA-0010). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Enhanced frequency of transcription pre-initiation complexes assemblyafter exposure to UV irradiation results in increased repair activity andreduced probabilities for mutagenesis. |
A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation. |
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No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). 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No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '585', 'name' => 'TBP Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'DA-0010', 'concentration' => '', 'reactivity' => 'Human, mouse', 'type' => 'Monoclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Ammonium sulphate purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>4 - 5 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>WB</td> <td>1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-04 15:39:37', 'created' => '2020-10-29 11:08:01', 'select_label' => '585 - TBP Antibody - ChIP-seq Grade (sample size) (DA-0010 - - Human, mouse - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '291', 'name' => 'C15200002 TBP Antibody', 'product_id' => '1960', 'modified' => '2020-10-29 11:13:43', 'created' => '2020-10-29 11:13:43' ), 'Master' => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4834', 'name' => 'Enhanced frequency of transcription pre-initiation complexes assemblyafter exposure to UV irradiation results in increased repair activity andreduced probabilities for mutagenesis.', 'authors' => 'Liakos A. et al.', 'description' => '<p>In addition to being essential for gene expression, transcription is crucial for the maintenance of genome integrity. Here, we undertook a systematic approach, to monitor the assembly kinetics of the pre-initiating RNA Polymerase (Pol) II at promoters at steady state and different stages during recovery from UV irradiation-stress, when pre-initiation and initiation steps have been suggested to be transiently shut down. Taking advantage of the reversible dissociation of pre-initiating Pol II after high salt treatment, we found that de novo recruitment of the available Pol II molecules at active promoters not only persists upon UV at all times tested but occurs significantly faster in the early phase of recovery (2 h) than in unexposed human fibroblasts at the majority of active genes. Our method unveiled groups of genes with significantly different pre-initiation complex (PIC) assembly dynamics after UV that present distinct rates of UV-related mutational signatures in melanoma tumours, providing functional relevance to the importance of keeping transcription initiation active during UV recovery. Our findings uncover novel mechanistic insights further detailing the multilayered transcriptional response to genotoxic stress and link PIC assembly dynamics after exposure to genotoxins with cancer mutational landscapes.</p>', 'date' => '2023-07-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37470822', 'doi' => '10.1093/nar/gkad593', 'modified' => '2023-08-01 13:47:31', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/tbp-monoclonal-antibody-classic-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'ProductsGroup' => array( 'id' => '329', 'product_id' => '3111', 'group_id' => '291' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-002-100)</span>' $country_code = 'US' $other_format = array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( 'id' => '5215', 'product_id' => '3111', 'application_id' => '45' ) ) $slugs = array( (int) 0 => 'sirna' ) $applications = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'locale' => 'eng' ) $description = '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>' $name = 'siRNA Knockdown' $document = array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '2949', 'product_id' => '3111', 'document_id' => '686' ) ) $publication = array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( 'id' => '6412', 'product_id' => '3111', 'publication_id' => '4559' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/35440565" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'product' => array( 'Product' => array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '585', 'name' => 'TBP Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'DA-0010', 'concentration' => '', 'reactivity' => 'Human, mouse', 'type' => 'Monoclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Ammonium sulphate purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>4 - 5 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>WB</td> <td>1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-04 15:39:37', 'created' => '2020-10-29 11:08:01', 'select_label' => '585 - TBP Antibody - ChIP-seq Grade (sample size) (DA-0010 - - Human, mouse - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/tbp-monoclonal-antibody-classic-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '585', 'name' => 'TBP Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'DA-0010', 'concentration' => '', 'reactivity' => 'Human, mouse', 'type' => 'Monoclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Ammonium sulphate purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>4 - 5 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>WB</td> <td>1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-04 15:39:37', 'created' => '2020-10-29 11:08:01', 'select_label' => '585 - TBP Antibody - ChIP-seq Grade (sample size) (DA-0010 - - Human, mouse - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '291', 'name' => 'C15200002 TBP Antibody', 'product_id' => '1960', 'modified' => '2020-10-29 11:13:43', 'created' => '2020-10-29 11:13:43' ), 'Master' => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4834', 'name' => 'Enhanced frequency of transcription pre-initiation complexes assemblyafter exposure to UV irradiation results in increased repair activity andreduced probabilities for mutagenesis.', 'authors' => 'Liakos A. et al.', 'description' => '<p>In addition to being essential for gene expression, transcription is crucial for the maintenance of genome integrity. Here, we undertook a systematic approach, to monitor the assembly kinetics of the pre-initiating RNA Polymerase (Pol) II at promoters at steady state and different stages during recovery from UV irradiation-stress, when pre-initiation and initiation steps have been suggested to be transiently shut down. Taking advantage of the reversible dissociation of pre-initiating Pol II after high salt treatment, we found that de novo recruitment of the available Pol II molecules at active promoters not only persists upon UV at all times tested but occurs significantly faster in the early phase of recovery (2 h) than in unexposed human fibroblasts at the majority of active genes. Our method unveiled groups of genes with significantly different pre-initiation complex (PIC) assembly dynamics after UV that present distinct rates of UV-related mutational signatures in melanoma tumours, providing functional relevance to the importance of keeping transcription initiation active during UV recovery. Our findings uncover novel mechanistic insights further detailing the multilayered transcriptional response to genotoxic stress and link PIC assembly dynamics after exposure to genotoxins with cancer mutational landscapes.</p>', 'date' => '2023-07-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37470822', 'doi' => '10.1093/nar/gkad593', 'modified' => '2023-08-01 13:47:31', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/tbp-monoclonal-antibody-classic-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'ProductsGroup' => array( 'id' => '329', 'product_id' => '3111', 'group_id' => '291' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-002-100)</span>' $country_code = 'US' $other_format = array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( 'id' => '5215', 'product_id' => '3111', 'application_id' => '45' ) ) $slugs = array( (int) 0 => 'sirna' ) $applications = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'locale' => 'eng' ) $description = '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>' $name = 'siRNA Knockdown' $document = array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '2949', 'product_id' => '3111', 'document_id' => '686' ) ) $publication = array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( 'id' => '6412', 'product_id' => '3111', 'publication_id' => '4559' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/35440565" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'product' => array( 'Product' => array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '585', 'name' => 'TBP Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'DA-0010', 'concentration' => '', 'reactivity' => 'Human, mouse', 'type' => 'Monoclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Ammonium sulphate purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>4 - 5 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>WB</td> <td>1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-04 15:39:37', 'created' => '2020-10-29 11:08:01', 'select_label' => '585 - TBP Antibody - ChIP-seq Grade (sample size) (DA-0010 - - Human, mouse - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/tbp-monoclonal-antibody-classic-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '585', 'name' => 'TBP Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'DA-0010', 'concentration' => '', 'reactivity' => 'Human, mouse', 'type' => 'Monoclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Ammonium sulphate purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>4 - 5 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>WB</td> <td>1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-04 15:39:37', 'created' => '2020-10-29 11:08:01', 'select_label' => '585 - TBP Antibody - ChIP-seq Grade (sample size) (DA-0010 - - Human, mouse - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '291', 'name' => 'C15200002 TBP Antibody', 'product_id' => '1960', 'modified' => '2020-10-29 11:13:43', 'created' => '2020-10-29 11:13:43' ), 'Master' => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4834', 'name' => 'Enhanced frequency of transcription pre-initiation complexes assemblyafter exposure to UV irradiation results in increased repair activity andreduced probabilities for mutagenesis.', 'authors' => 'Liakos A. et al.', 'description' => '<p>In addition to being essential for gene expression, transcription is crucial for the maintenance of genome integrity. Here, we undertook a systematic approach, to monitor the assembly kinetics of the pre-initiating RNA Polymerase (Pol) II at promoters at steady state and different stages during recovery from UV irradiation-stress, when pre-initiation and initiation steps have been suggested to be transiently shut down. Taking advantage of the reversible dissociation of pre-initiating Pol II after high salt treatment, we found that de novo recruitment of the available Pol II molecules at active promoters not only persists upon UV at all times tested but occurs significantly faster in the early phase of recovery (2 h) than in unexposed human fibroblasts at the majority of active genes. Our method unveiled groups of genes with significantly different pre-initiation complex (PIC) assembly dynamics after UV that present distinct rates of UV-related mutational signatures in melanoma tumours, providing functional relevance to the importance of keeping transcription initiation active during UV recovery. Our findings uncover novel mechanistic insights further detailing the multilayered transcriptional response to genotoxic stress and link PIC assembly dynamics after exposure to genotoxins with cancer mutational landscapes.</p>', 'date' => '2023-07-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37470822', 'doi' => '10.1093/nar/gkad593', 'modified' => '2023-08-01 13:47:31', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/tbp-monoclonal-antibody-classic-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'ProductsGroup' => array( 'id' => '329', 'product_id' => '3111', 'group_id' => '291' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-002-100)</span>' $country_code = 'US' $other_format = array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( 'id' => '5215', 'product_id' => '3111', 'application_id' => '45' ) ) $slugs = array( (int) 0 => 'sirna' ) $applications = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'locale' => 'eng' ) $description = '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>' $name = 'siRNA Knockdown' $document = array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '2949', 'product_id' => '3111', 'document_id' => '686' ) ) $publication = array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( 'id' => '6412', 'product_id' => '3111', 'publication_id' => '4559' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/35440565" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-04 15:39:37', 'created' => '2020-10-29 11:08:01', 'select_label' => '585 - TBP Antibody - ChIP-seq Grade (sample size) (DA-0010 - - Human, mouse - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/tbp-monoclonal-antibody-classic-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '585', 'name' => 'TBP Antibody - ChIP-seq Grade (sample size)', 'description' => '', 'clonality' => '', 'isotype' => '', 'lot' => 'DA-0010', 'concentration' => '', 'reactivity' => 'Human, mouse', 'type' => 'Monoclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Ammonium sulphate purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>4 - 5 μg/IP</td> <td>Fig 1, 2</td> </tr> <tr> <td>WB</td> <td>1:500</td> <td>Fig 3</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-11-04 15:39:37', 'created' => '2020-10-29 11:08:01', 'select_label' => '585 - TBP Antibody - ChIP-seq Grade (sample size) (DA-0010 - - Human, mouse - Ammonium sulphate purified - Mouse)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '291', 'name' => 'C15200002 TBP Antibody', 'product_id' => '1960', 'modified' => '2020-10-29 11:13:43', 'created' => '2020-10-29 11:13:43' ), 'Master' => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 4 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4834', 'name' => 'Enhanced frequency of transcription pre-initiation complexes assemblyafter exposure to UV irradiation results in increased repair activity andreduced probabilities for mutagenesis.', 'authors' => 'Liakos A. et al.', 'description' => '<p>In addition to being essential for gene expression, transcription is crucial for the maintenance of genome integrity. Here, we undertook a systematic approach, to monitor the assembly kinetics of the pre-initiating RNA Polymerase (Pol) II at promoters at steady state and different stages during recovery from UV irradiation-stress, when pre-initiation and initiation steps have been suggested to be transiently shut down. Taking advantage of the reversible dissociation of pre-initiating Pol II after high salt treatment, we found that de novo recruitment of the available Pol II molecules at active promoters not only persists upon UV at all times tested but occurs significantly faster in the early phase of recovery (2 h) than in unexposed human fibroblasts at the majority of active genes. Our method unveiled groups of genes with significantly different pre-initiation complex (PIC) assembly dynamics after UV that present distinct rates of UV-related mutational signatures in melanoma tumours, providing functional relevance to the importance of keeping transcription initiation active during UV recovery. Our findings uncover novel mechanistic insights further detailing the multilayered transcriptional response to genotoxic stress and link PIC assembly dynamics after exposure to genotoxins with cancer mutational landscapes.</p>', 'date' => '2023-07-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37470822', 'doi' => '10.1093/nar/gkad593', 'modified' => '2023-08-01 13:47:31', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/tbp-monoclonal-antibody-classic-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3111', 'antibody_id' => '585', 'name' => 'TBP Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '10 µg', 'catalog_number' => 'C15200002-10', 'old_catalog_number' => '', 'sf_code' => 'C15200002-D001-000582', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'tbp-monoclonal-antibody-classic-10', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 14:58:18', 'created' => '2020-10-29 11:12:44', 'ProductsGroup' => array( 'id' => '329', 'product_id' => '3111', 'group_id' => '291' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(MAb-002-100)</span>' $country_code = 'US' $other_format = array( 'id' => '1960', 'antibody_id' => '313', 'name' => 'TBP Antibody', 'description' => '<p>Alternative names: <strong>GTF2D</strong>, <strong>GTF2D1</strong>, <strong>SCA17</strong>, <strong>TF2D</strong>, <strong>TFIID</strong></p> <p><span>Monoclonal antibody raised in mouse against the amino-terminal domain of human <strong>TBP (TATA box binding protein).</strong></span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-1.png" alt="TBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></div> <div class="small-8 columns"> <p><small><strong>Figure 1 ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TBP </strong><br />ChIP was performed with 5 μg of the Diagenode antibody against TBP (Cat. No. C15200002) on sheared chromatin from 1 million HeLaS3 cells using the “Auto Histone ChIP-seq” kit (Cat. No. C01010022) on the IP-Star automated system. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (figure 2A). The IP’d DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.</small></p> </div> </div> <div class="row"> <div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-2.png" alt="TBP Antibody - ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /><br /> B. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-3.png" alt="TBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /><br /> C. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-4.png" alt="TBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /><br /> D. <img src="https://www.diagenode.com/img/product/antibodies/C15200002-chipseq-5.png" alt="TBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></div> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="spacer"> <p></p> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-chip.png" alt="TBP Antibody for ChIP assay" width="269" height="340" caption="false" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2 ChIP results obtained with the Diagenode monoclonal antibody against TBP </strong><br />ChIP assays were performed using U2OS cells, the Diagenode antibody directed against TBP (Cat. No. C15200002) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 μg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes (Cat. No. C17011004 and C17011001), a region 0.5 and 1 kb upstream of the GAPDH promoter (Cat. No. C17011002 and C17011003), respectively, and for exon 2 of the myoglobin gene (cat. No. C17011006) as a negative control. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200002-wb.png" alt="TBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against TBP</strong><br />Whole cell extracts (40 μg) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against TBP (Cat. No. C15200002) diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200002', 'old_catalog_number' => 'MAb-002-100', 'sf_code' => 'C15200002-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 17, 2017', 'slug' => 'tbp-monoclonal-antibody-classic-100-ul', 'meta_title' => 'TBP Antibody - ChIP-seq Grade (C15200002) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TBP (TATA box binding protein) Monoclonal Antibody validated in ChIP-seq, ChIP-qPCR and WB. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: GTF2D, GTF2D1, SCA17, TF2D, TFIID', 'modified' => '2022-01-05 14:58:08', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'ProductsApplication' => array( 'id' => '5215', 'product_id' => '3111', 'application_id' => '45' ) ) $slugs = array( (int) 0 => 'sirna' ) $applications = array( 'id' => '45', 'position' => '10', 'parent_id' => '40', 'name' => 'siRNA Knockdown', 'description' => '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'sirna', 'meta_keywords' => 'siRNA knockdown antibodies', 'meta_description' => 'Antibodies validated by siRNA Knockdown', 'meta_title' => '', 'modified' => '2017-01-26 15:58:23', 'created' => '2017-01-09 10:53:09', 'locale' => 'eng' ) $description = '<div class="row"> <div class="small-10 columns"> <h3>Epigenetic antibodies you can trust!</h3> <p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p> <p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p> <center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center> <p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p> </div> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p> </div> </div> <div class="spaced"></div> <p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>' $name = 'siRNA Knockdown' $document = array( 'id' => '686', 'name' => 'Datasheet TBP C15200002', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TBP_C15200002.pdf', 'slug' => 'datasheet-tbp-C15200002', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-20 17:18:31', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '2949', 'product_id' => '3111', 'document_id' => '686' ) ) $publication = array( 'id' => '4559', 'name' => 'A leukemia-protective germline variant mediates chromatin moduleformation via transcription factor nucleation.', 'authors' => 'Llimos G. et al.', 'description' => '<p>Non-coding variants coordinate transcription factor (TF) binding and chromatin mark enrichment changes over regions spanning >100 kb. These molecularly coordinated regions are named "variable chromatin modules" (VCMs), providing a conceptual framework of how regulatory variation might shape complex traits. To better understand the molecular mechanisms underlying VCM formation, here, we mechanistically dissect a VCM-modulating noncoding variant that is associated with reduced chronic lymphocytic leukemia (CLL) predisposition and disease progression. This common, germline variant constitutes a 5-bp indel that controls the activity of an AXIN2 gene-linked VCM by creating a MEF2 binding site, which, upon binding, activates a super-enhancer-like regulatory element. This triggers a large change in TF binding activity and chromatin state at an enhancer cluster spanning >150 kb, coinciding with subtle, long-range chromatin compaction and robust AXIN2 up-regulation. Our results support a model in which the indel acts as an AXIN2 VCM-activating TF nucleation event, which modulates CLL pathology.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35440565', 'doi' => '10.1038/s41467-022-29625-6', 'modified' => '2022-11-24 10:00:25', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( 'id' => '6412', 'product_id' => '3111', 'publication_id' => '4559' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/35440565" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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