Protein Extraction beads

Catalog Number

Format

Price

C20000021
(AL-003-0020)

20 g

$100.00

Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.

Characteristics
The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction beads offers unique benefits for tissue disruption and homogenization:
- Fast and simple
- No contamination between samples
- Efficient
- Gentle processing
- Reproducible
- Temperature controlled
- Multiplexing capability

Documents
Protein Extraction beads DATASHEET
The protein extraction beads C20000021 have been extensively validated to be us... Download

Publications

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p73 regulates epidermal wound healing and induced keratinocyte programming.
Beeler JS, Marshall CB, Gonzalez-Ericsson PI, Shaver TM, Santos Guasch GL, Lea ST, Johnson KN, Jin H, Venters BJ, Sanders ME, Pietenpol JA
p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that is expressed in select p63-positive basal cells and can heterodimerize with p63. p73-/- mice lack multiciliated cells and have reduced ...

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<p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p>
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<p><span>The protein extraction beads&nbsp;</span><strong>C20000021&nbsp;</strong><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">have been extensively validated to be used in combination with Diagenode&rsquo;s Bioruptor</span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">&reg; </span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">Standard, Plus and Pico to ensure efficient disruption and homogenization of fresh and frozen tissues. The Protein Extraction Beads guarantee high yields of proteins.</span></p>
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            <h6 style="height:60px">15 ml Bioruptor® Pico Tubes</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200016_tube_holder.jpg" alt="some alt" class="th"/></a>        </div>



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                <span class="success label" style="">B01200016</span>
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<form action="/en/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/>
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        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml sonication accessories for Bioruptor<sup>&reg;</sup> Standard & Plus & Pico</strong>  to my shopping cart.</p>

        <div class="row">
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                <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>&reg;</sup> Standard & Plus & Pico',
                    'B01200016',
                     '350',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>&reg;</sup> Standard & Plus & Pico',
                    'B01200016',
                    '350',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/1-5-ml-tpx-microtubes-50-pc"><img src="/img/product/shearing_technologies/C30010010_tube.png" alt="some alt" class="th"/></a>        </div>



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            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2623" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2623" id="CartAdd/2623Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2623" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 1.5 ml Bioruptor® Plus TPX microtubes</strong>  to my shopping cart.</p>

        <div class="row">
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                <button class="alert small button expand" onclick="$(this).addToCart('1.5 ml Bioruptor® Plus TPX microtubes',
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                     '55',
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            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('1.5 ml Bioruptor® Plus TPX microtubes',
                    'C30010010-50',
                    '55',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="1-5-ml-tpx-microtubes-50-pc" data-reveal-id="cartModal-2623" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">1.5 ml Bioruptor® Plus TPX microtubes</h6>
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    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes"><img src="/img/product/shearing_technologies/C30010016_tube.png" alt="some alt" class="th"/></a>        </div>



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            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
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                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
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<form action="/en/carts/add/2630" id="CartAdd/2630Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2630" id="CartProductId"/>
<div class="row">
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        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 1.5 ml Picoruptor Microtubes with Caps</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('1.5 ml Picoruptor Microtubes with Caps',
                    'C30010016',
                     '150',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('1.5 ml Picoruptor Microtubes with Caps',
                    'C30010016',
                    '150',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="1-5-ml-bioruptor-microtubes-with-caps-300-tubes" data-reveal-id="cartModal-2630" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">1.5 ml Bioruptor&reg; Pico Microtubes with Caps</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack"><img src="/img/product/shearing_technologies/B01200040_tube_holder.jpg" alt="some alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
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<form action="/en/carts/add/1792" id="CartAdd/1792Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1792" id="CartProductId"/>
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                    'B01200011',
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                    'B01200011',
                    '1850',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack" data-reveal-id="cartModal-1792" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">1.5 ml tube holder for Bioruptor&reg; Standard ...</h6>
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    </div>
</li>
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	'type' => 'Protocol',
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<div class="section">
<div class="layoutArea">
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<p><span>The protein extraction beads&nbsp;</span><strong>C20000021&nbsp;</strong><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">have been extensively validated to be used in combination with Diagenode&rsquo;s Bioruptor</span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">&reg; </span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">Standard, Plus and Pico to ensure efficient disruption and homogenization of fresh and frozen tissues. The Protein Extraction Beads guarantee high yields of proteins.</span></p>
</div>
</div>
</div>
</div>',
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		'product_id' => '2618',
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	'id' => '3740',
	'name' => 'p73 regulates epidermal wound healing and induced keratinocyte programming.',
	'authors' => 'Beeler JS, Marshall CB, Gonzalez-Ericsson PI, Shaver TM, Santos Guasch GL, Lea ST, Johnson KN, Jin H, Venters BJ, Sanders ME, Pietenpol JA',
	'description' => '<p>p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that is expressed in select p63-positive basal cells and can heterodimerize with p63. p73-/- mice lack multiciliated cells and have reduced numbers of basal epithelial cells in select tissues; however, the role of p73 in basal epithelial cells is unknown. Herein, we show that p73-deficient mice exhibit delayed wound healing despite morphologically normal-appearing skin. The delay in wound healing is accompanied by decreased proliferation and increased levels of biomarkers of the DNA damage response in basal keratinocytes at the epidermal wound edge. In wild-type mice, this same cell population exhibited increased p73 expression after wounding. Analyzing single-cell transcriptomic data, we found that p73 was expressed by epidermal and hair follicle stem cells, cell types required for wound healing. Moreover, we discovered that p73 isoforms expressed in the skin (&Delta;Np73) enhance p63-mediated expression of keratinocyte genes during cellular reprogramming from a mesenchymal to basal keratinocyte-like cell. We identified a set of 44 genes directly or indirectly regulated by &Delta;Np73 that are involved in skin development, cell junctions, cornification, proliferation, and wound healing. Our results establish a role for p73 in cutaneous wound healing through regulation of basal keratinocyte function.</p>',
	'date' => '2019-06-19',
	'pmid' => 'http://www.pubmed.gov/31216312',
	'doi' => '10.1371/journal.pone.0218458',
	'modified' => '2019-08-06 16:55:36',
	'created' => '2019-07-31 13:35:50',
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)
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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	'meta_description' => 'Protein Extraction beads',
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<ul>
<li>Fast and simple</li>
<li>No contamination between samples</li>
<li>Efficient</li>
<li>Gentle processing</li>
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<li>Multiplexing capability</li>
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<div class="large-12 columns">Diagenode offers optimized protein extraction kits for use with the Bioruptor<sup><span>&reg;</span> </sup>sonication devices. The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor<sup><span>&reg;</span></sup>.</div>
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<p><span>The protein extraction beads&nbsp;</span><strong>C20000021&nbsp;</strong><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">have been extensively validated to be used in combination with Diagenode&rsquo;s Bioruptor</span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">&reg; </span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">Standard, Plus and Pico to ensure efficient disruption and homogenization of fresh and frozen tissues. The Protein Extraction Beads guarantee high yields of proteins.</span></p>
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			'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode&rsquo;s Bioruptor</span><span>&reg; </span><span>Pico uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>',
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			'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode&rsquo;s Bioruptor</span><span>&reg; </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>',
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			'description' => '<p>p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that is expressed in select p63-positive basal cells and can heterodimerize with p63. p73-/- mice lack multiciliated cells and have reduced numbers of basal epithelial cells in select tissues; however, the role of p73 in basal epithelial cells is unknown. Herein, we show that p73-deficient mice exhibit delayed wound healing despite morphologically normal-appearing skin. The delay in wound healing is accompanied by decreased proliferation and increased levels of biomarkers of the DNA damage response in basal keratinocytes at the epidermal wound edge. In wild-type mice, this same cell population exhibited increased p73 expression after wounding. Analyzing single-cell transcriptomic data, we found that p73 was expressed by epidermal and hair follicle stem cells, cell types required for wound healing. Moreover, we discovered that p73 isoforms expressed in the skin (&Delta;Np73) enhance p63-mediated expression of keratinocyte genes during cellular reprogramming from a mesenchymal to basal keratinocyte-like cell. We identified a set of 44 genes directly or indirectly regulated by &Delta;Np73 that are involved in skin development, cell junctions, cornification, proliferation, and wound healing. Our results establish a role for p73 in cutaneous wound healing through regulation of basal keratinocyte function.</p>',
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<div class="large-12 columns">Diagenode offers optimized protein extraction kits for use with the Bioruptor<sup><span>&reg;</span> </sup>sonication devices. The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor<sup><span>&reg;</span></sup>.</div>
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<p><span>The protein extraction beads&nbsp;</span><strong>C20000021&nbsp;</strong><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">have been extensively validated to be used in combination with Diagenode&rsquo;s Bioruptor</span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">&reg; </span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">Standard, Plus and Pico to ensure efficient disruption and homogenization of fresh and frozen tissues. The Protein Extraction Beads guarantee high yields of proteins.</span></p>
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			'authors' => 'Beeler JS, Marshall CB, Gonzalez-Ericsson PI, Shaver TM, Santos Guasch GL, Lea ST, Johnson KN, Jin H, Venters BJ, Sanders ME, Pietenpol JA',
			'description' => '<p>p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that is expressed in select p63-positive basal cells and can heterodimerize with p63. p73-/- mice lack multiciliated cells and have reduced numbers of basal epithelial cells in select tissues; however, the role of p73 in basal epithelial cells is unknown. Herein, we show that p73-deficient mice exhibit delayed wound healing despite morphologically normal-appearing skin. The delay in wound healing is accompanied by decreased proliferation and increased levels of biomarkers of the DNA damage response in basal keratinocytes at the epidermal wound edge. In wild-type mice, this same cell population exhibited increased p73 expression after wounding. Analyzing single-cell transcriptomic data, we found that p73 was expressed by epidermal and hair follicle stem cells, cell types required for wound healing. Moreover, we discovered that p73 isoforms expressed in the skin (&Delta;Np73) enhance p63-mediated expression of keratinocyte genes during cellular reprogramming from a mesenchymal to basal keratinocyte-like cell. We identified a set of 44 genes directly or indirectly regulated by &Delta;Np73 that are involved in skin development, cell junctions, cornification, proliferation, and wound healing. Our results establish a role for p73 in cutaneous wound healing through regulation of basal keratinocyte function.</p>',
			'date' => '2019-06-19',
			'pmid' => 'http://www.pubmed.gov/31216312',
			'doi' => '10.1371/journal.pone.0218458',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><span>The protein extraction beads&nbsp;</span><strong>C20000021&nbsp;</strong><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">have been extensively validated to be used in combination with Diagenode&rsquo;s Bioruptor</span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">&reg; </span><span style="font-family: inherit; font-size: 1rem; line-height: 1.6;">Standard, Plus and Pico to ensure efficient disruption and homogenization of fresh and frozen tissues. The Protein Extraction Beads guarantee high yields of proteins.</span></p>
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			'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode&rsquo;s Bioruptor</span><span>&reg; </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>',
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			'description' => '<p>p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that is expressed in select p63-positive basal cells and can heterodimerize with p63. p73-/- mice lack multiciliated cells and have reduced numbers of basal epithelial cells in select tissues; however, the role of p73 in basal epithelial cells is unknown. Herein, we show that p73-deficient mice exhibit delayed wound healing despite morphologically normal-appearing skin. The delay in wound healing is accompanied by decreased proliferation and increased levels of biomarkers of the DNA damage response in basal keratinocytes at the epidermal wound edge. In wild-type mice, this same cell population exhibited increased p73 expression after wounding. Analyzing single-cell transcriptomic data, we found that p73 was expressed by epidermal and hair follicle stem cells, cell types required for wound healing. Moreover, we discovered that p73 isoforms expressed in the skin (&Delta;Np73) enhance p63-mediated expression of keratinocyte genes during cellular reprogramming from a mesenchymal to basal keratinocyte-like cell. We identified a set of 44 genes directly or indirectly regulated by &Delta;Np73 that are involved in skin development, cell junctions, cornification, proliferation, and wound healing. Our results establish a role for p73 in cutaneous wound healing through regulation of basal keratinocyte function.</p>',
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</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-50-pc" data-reveal-id="cartModal-2631" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="1-5-ml-bioruptor-microtubes-with-caps-300-tubes" data-reveal-id="cartModal-2630" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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    </div>
</li>
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	'name' => 'p73 regulates epidermal wound healing and induced keratinocyte programming.',
	'authors' => 'Beeler JS, Marshall CB, Gonzalez-Ericsson PI, Shaver TM, Santos Guasch GL, Lea ST, Johnson KN, Jin H, Venters BJ, Sanders ME, Pietenpol JA',
	'description' => '<p>p63 is a transcriptional regulator of ectodermal development that is required for basal cell proliferation and stem cell maintenance. p73 is a closely related p53 family member that is expressed in select p63-positive basal cells and can heterodimerize with p63. p73-/- mice lack multiciliated cells and have reduced numbers of basal epithelial cells in select tissues; however, the role of p73 in basal epithelial cells is unknown. Herein, we show that p73-deficient mice exhibit delayed wound healing despite morphologically normal-appearing skin. The delay in wound healing is accompanied by decreased proliferation and increased levels of biomarkers of the DNA damage response in basal keratinocytes at the epidermal wound edge. In wild-type mice, this same cell population exhibited increased p73 expression after wounding. Analyzing single-cell transcriptomic data, we found that p73 was expressed by epidermal and hair follicle stem cells, cell types required for wound healing. Moreover, we discovered that p73 isoforms expressed in the skin (&Delta;Np73) enhance p63-mediated expression of keratinocyte genes during cellular reprogramming from a mesenchymal to basal keratinocyte-like cell. We identified a set of 44 genes directly or indirectly regulated by &Delta;Np73 that are involved in skin development, cell junctions, cornification, proliferation, and wound healing. Our results establish a role for p73 in cutaneous wound healing through regulation of basal keratinocyte function.</p>',
	'date' => '2019-06-19',
	'pmid' => 'http://www.pubmed.gov/31216312',
	'doi' => '10.1371/journal.pone.0218458',
	'modified' => '2019-08-06 16:55:36',
	'created' => '2019-07-31 13:35:50',
	'ProductsPublication' => array(
		'id' => '3959',
		'product_id' => '2618',
		'publication_id' => '3740'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/31216312" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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