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Other name : Cas13a
Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR associated protein 13a) using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1 µg/µl |
| Species reactivity | Leptotrichia shahii |
| Type | Monoclonal |
| Purity | Protein G purified monoclonal antibody |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
| Storage Buffer | PBS containing 0.05 % Na-azide |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
|
Applications |
Suggested dilution/amount |
References |
|
Western blotting |
1:1,000 |
Fig 1 |
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against C2C2
Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. The marker is shown on the left, the position of the C2C2 protein is indicated on the right.
| C2C2 monoclonal antibody datasheet DATASHEET Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR ass... | Download |
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Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. The marker is shown on the left, the position of the C2C2 protein is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C15200249', 'old_catalog_number' => 'C15200241', 'sf_code' => 'C15200249-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '445', 'price_USD' => '465', 'price_GBP' => '420', 'price_JPY' => '69710', 'price_CNY' => '/', 'price_AUD' => '1162', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 13, 2019', 'slug' => 'c2c2-monoclonal-antibody-50-ug', 'meta_title' => 'C2C2 Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'C2C2 (CRISPR associated protein 13a) Monoclonal Antibody validated in WB. Batch-specific data available on the website. 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Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'C2C2 (CRISPR associated protein 13a) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Other name : Cas13a', 'meta_title' => 'C2C2 Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2994', 'antibody_id' => '544', 'name' => 'C2C2 Antibody', 'description' => '<p><strong>Other name : Cas13a</strong></p> <p>Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR associated protein 13a) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200241-WB.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" width="167" height="290" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Batch-specific data available on the website. Other name : Cas13a' $meta_title = 'C2C2 Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2994', 'antibody_id' => '544', 'name' => 'C2C2 Antibody', 'description' => '<p><strong>Other name : Cas13a</strong></p> <p>Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR associated protein 13a) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200241-WB.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" width="167" height="290" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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C2C2 (Cas13a) may be useful for isolating specific RNA sequences or even studying single RNA molecules with high specificity. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'C2C2 (CRISPR associated protein 13a) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Other name : Cas13a', 'meta_title' => 'C2C2 Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2994', 'antibody_id' => '544', 'name' => 'C2C2 Antibody', 'description' => '<p><strong>Other name : Cas13a</strong></p> <p>Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR associated protein 13a) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200241-WB.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" width="167" height="290" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified in Leptotrichia shahii. This CRISPR system is characterized by the presence of the single effector protein C2C2. C2C2 lacks homology to any known DNA nuclease domain but contains two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and is thought to function solely as an RNA-guided RNA-targeting CRISPR effector.', 'clonality' => '', 'isotype' => 'IgG1', 'lot' => '001', 'concentration' => '1 µg/µl', 'reactivity' => 'Leptotrichia shahii', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<p><strong> </strong></p> <table> <tbody> <tr> <td width="158"> <p><strong>Applications</strong></p> </td> <td width="153"> <p><strong>Suggested dilution/amount</strong></p> </td> <td width="99"> <p><strong>References</strong></p> </td> </tr> <tr> <td width="158"> <p>Western blotting</p> </td> <td width="153"> <p>1:1,000</p> </td> <td width="99"> <p>Fig 1</p> </td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. 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Batch-specific data available on the website. Other name : Cas13a' $meta_title = 'C2C2 Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2994', 'antibody_id' => '544', 'name' => 'C2C2 Antibody', 'description' => '<p><strong>Other name : Cas13a</strong></p> <p>Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR associated protein 13a) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200241-WB.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" width="167" height="290" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. The marker is shown on the left, the position of the C2C2 protein is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C15200249', 'old_catalog_number' => 'C15200241', 'sf_code' => 'C15200249-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '445', 'price_USD' => '465', 'price_GBP' => '420', 'price_JPY' => '69710', 'price_CNY' => '/', 'price_AUD' => '1162', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 13, 2019', 'slug' => 'c2c2-monoclonal-antibody-50-ug', 'meta_title' => 'C2C2 Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'C2C2 (CRISPR associated protein 13a) Monoclonal Antibody validated in WB. Batch-specific data available on the website. 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In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified in Leptotrichia shahii. This CRISPR system is characterized by the presence of the single effector protein C2C2. C2C2 lacks homology to any known DNA nuclease domain but contains two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and is thought to function solely as an RNA-guided RNA-targeting CRISPR effector.', 'clonality' => '', 'isotype' => 'IgG1', 'lot' => '001', 'concentration' => '1 µg/µl', 'reactivity' => 'Leptotrichia shahii', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<p><strong> </strong></p> <table> <tbody> <tr> <td width="158"> <p><strong>Applications</strong></p> </td> <td width="153"> <p><strong>Suggested dilution/amount</strong></p> </td> <td width="99"> <p><strong>References</strong></p> </td> </tr> <tr> <td width="158"> <p>Western blotting</p> </td> <td width="153"> <p>1:1,000</p> </td> <td width="99"> <p>Fig 1</p> </td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. 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Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '134', 'position' => '0', 'parent_id' => '100', 'name' => 'RNA-targeting CRISPR antibodies', 'description' => '<p>The latest tool for CRISPR gene editing is a new CRISPR-C2C2 (Cas13a) system which utilizes RNA-guided RNA-targeting for RNA knockdown and binding in cells. C2C2 (Cas13a) may be useful for isolating specific RNA sequences or even studying single RNA molecules with high specificity. Unlike editing DNA as with Cas9 which makes permanent genomic changes, targeting RNA with C2C2 allows temporarily altering the amount of protein potentially offering a better therapeutic approach in the future. </p> <p>Use Diagenode's new C2C2 (Cas13a) polyclonal antibody to determine precision and success metrics in CRISPR experiments.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'antibodies-against-rna-targeting-crispr', 'cookies_tag_id' => '1', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Antibodies against RNA-targeting CRISPR', 'modified' => '2018-04-26 15:44:54', 'created' => '2018-03-05 11:46:15', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array( [maximum depth reached] ) ) ), 'Document' => array( (int) 0 => array( 'id' => '1027', 'name' => 'C2C2 monoclonal antibody datasheet', 'description' => '<p>Monoclonal antibody 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'C2C2 (CRISPR associated protein 13a) Monoclonal Antibody validated in WB. Batch-specific data available on the website. Other name : Cas13a', 'meta_title' => 'C2C2 Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2994', 'antibody_id' => '544', 'name' => 'C2C2 Antibody', 'description' => '<p><strong>Other name : Cas13a</strong></p> <p>Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR associated protein 13a) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200241-WB.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" width="167" height="290" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. The marker is shown on the left, the position of the C2C2 protein is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C15200249', 'old_catalog_number' => 'C15200241', 'sf_code' => 'C15200249-D001-000581', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '445', 'price_USD' => '465', 'price_GBP' => '420', 'price_JPY' => '69710', 'price_CNY' => '/', 'price_AUD' => '1162', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 13, 2019', 'slug' => 'c2c2-monoclonal-antibody-50-ug', 'meta_title' => 'C2C2 Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'C2C2 (CRISPR associated protein 13a) Monoclonal Antibody validated in WB. Batch-specific data available on the website. 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Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>' $name = 'WB' $document = array( 'id' => '1027', 'name' => 'C2C2 monoclonal antibody datasheet', 'description' => '<p>Monoclonal antibody raised in mouse against the C2C2 protein from Leptotrichia shahii (CRISPR associated protein 13a) using a recombinant protein.</p> <p><strong>Other name : Cas13a</strong></p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => '../files/products/antibodies/Datasheet_C15200241_C2C2.pdf', 'slug' => 'c2c2-monoclonal-antibody-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-09-13 14:49:32', 'created' => '2018-09-13 14:49:32', 'ProductsDocument' => array( 'id' => '2672', 'product_id' => '2994', 'document_id' => '1027' ) ) $sds = array( 'id' => '1586', 'name' => 'C2C2 antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/C2C2/SDS-C15200249-C2C2_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2021-08-30 17:13:51', 'created' => '2021-08-30 17:13:51', 'ProductsSafetySheet' => array( 'id' => '2762', 'product_id' => '2994', 'safety_sheet_id' => '1586' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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