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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against C2C2</strong><br />Western blot was performed on protein extracts from U2OS cells spiked with a partial recombinant C2C2 protein (430 AA, lane 1), using the Diagenode antibody against C2C2 (Cat. No. C15200249), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. The marker is shown on the left, the position of the C2C2 protein is indicated on the right.</small></p>
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'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified in Leptotrichia shahii. This CRISPR system is characterized by the presence of the single effector protein C2C2. C2C2 lacks homology to any known DNA nuclease domain but contains two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and is thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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