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Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI
Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.
| Lot | A386-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:1,000 | Fig 1 |
| Western Blotting | 1:500 | Fig 2 (1) |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.
Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.
DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ELISA Enzyme-linked immunosorbent assay. Read more |
| Datasheet DNMT1 CS-111-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: DNMT1 Antibody (Diagenode Cat# C15310111 Lot# A386-004). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. 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Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.', 'clonality' => '', 'isotype' => '', 'lot' => 'A386-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2 (1)</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = '' $meta_description = 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ' $meta_title = 'DNMT1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2101', 'antibody_id' => '199', 'name' => 'DNMT1 Antibody ', 'description' => '<p><span>Alternative names: <strong>Dnmt</strong>, <strong>Met1</strong>, <strong>Met-1</strong>, <strong>Uim</strong>, <strong>MCMT</strong>, <strong>M.MmuI</strong> </span></p> <p><span>Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig1.jpg" alt="ELISA" height="237" width="297" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310111', 'old_catalog_number' => 'CS-111-100', 'sf_code' => 'C15310111-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2241', 'antibody_id' => '152', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human DNMT3A (Cat. No. pAb-084-050), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:500. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-084-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/67 µl', 'catalog_number' => 'C15410084', 'old_catalog_number' => 'pAb-084-050', 'sf_code' => 'C15410084-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-67-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. ', 'modified' => '2022-01-05 15:30:56', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2242', 'antibody_id' => '153', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 92-106.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-085-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-085-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/54 µl', 'catalog_number' => 'C15410085', 'old_catalog_number' => 'pAb-085-050', 'sf_code' => 'C15410085-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-54-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in IP, WB and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:33:31', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2243', 'antibody_id' => '154', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 107-121.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-086-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:400. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-086-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/64 µl', 'catalog_number' => 'C15410086', 'old_catalog_number' => 'pAb-086-050', 'sf_code' => 'C15410086-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-64-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:31:07', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2294', 'antibody_id' => '157', 'name' => 'DNMT3B Antibody ', 'description' => '<p>Alternative names: <strong>Dnmt3b</strong>, <strong>DNA MTase HsaIIIB</strong>, <strong>M.HsaIIIB</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using 3 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_ELISA.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against DNMT3B (Cat. No. C15410218). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:220,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT3B</strong><br /> Whole cell extracts (25 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody against DNMT3B (Cat. No. C15410218) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 3. Immunofluorescence using the Diagenode antibody directed against DNMT3B</strong><br /> Human HeLa cells were stained with the Diagenode antibody against DNMT3B (Cat. No. C15410218) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3B (UniProtKB/Swiss-Prot entry Q9UBC3) catalyses the genome wide de novo methylation of CpG residues, which regulates gene expression. DNMT3B is essential for development. DNA methylation on CpG residues is coordinated with methylation of histones. Six different isoforms of DNMT3B, produced by alternative splicing, exist although isoforms 4 and 5 may not be functional due to the absence of two conserved methyltransferase motifs.</p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg/ 16 μl', 'catalog_number' => 'C15410218', 'old_catalog_number' => '', 'sf_code' => 'C15410218-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3b-polyclonal-antibody-classic-50-mg-16-ml', 'meta_title' => 'DNMT3B Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3B (DNA methyltransferase 3B) Polyclonal Antibody validated in IF, WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB', 'modified' => '2024-01-17 17:55:24', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1980', 'antibody_id' => '630', 'name' => '5-methylcytosine (5-mC) Antibody - clone 33D3', 'description' => '<p><span>Monoclonal antibody raised in mouse against </span><b>5-mC</b><span><span> </span>(</span><b>5-methylcytosine</b><span>) conjugated to ovalbumine (</span><b>33D3 clone</b><span>).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-12 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-A.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="173" /></p> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-B.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="184" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 1. MeDIP-seq with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> Genomic DNA from E14 ES cells was sheared with the Bioruptor® to generate random fragments (size range 300 to 700 bp). One µg of the fragmented DNA was ligated to Illumina adapters and the resulting DNA was used for a standard MeDIP assay, using 2 µg of the Diagenode monoclonal against 5-mC (Cat. No. C15200081). After recovery of the methylated DNA, Illumina sequencing libraries were generated and sequenced on an Illumina Genome Analyzer according to the manufacturer’s instructions. Figure 1A and 1B show Genome browser views of CA simple repeat elements with read distributions specific for 5-mC at 2 gene locations (SigleC15 and Mfsd4). Visual inspection of the peak profiles in a genome browser reveals high enrichment of CA simple repeats in affinity-enriched methylated fragments after MeDIP with the Diagenode 5-mC monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_medip.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP" caption="false" width="355" height="372" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. MeDIP results obtained with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> MeDIP (Methylated DNA immunoprecipitation) was performed on 1 µg fragmented human genomic DNA using 0.2 µg of the Diagenode monoclonal antibody against 5-mC (cat. No. C15200081) and the MagMeDIP Kit (cat. No. C02010021). The fragmented DNA was spiked with the internal controls present in the kit (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with optimized primer sets, included in the kit, specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. Figure 2 shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_Dotblot.png" alt=" 5-mC (5-methylcytosine) Antibody validated in dot blot" caption="false" width="201" height="196" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against 5-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 5-mC (cat. No. C15200081), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (cat. No. C02040010). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane. Figure 3 shows a high specificity of the antibody for the methylated control.</small></p> </div> </div> <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_IF1.png" alt="5-mC (5-methylcytosine) Antibody for immunofluorescence" height="121" width="500" caption="false" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against 5-mC</strong><br />HeLa cells were stained with the Diagenode antibody against 5-mC (Cat. No. C15200081) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p> </div> </div> <!-- <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_SPR.png" alt="5-methylcytosine (5-mC) Antibody" surface="" plasmon="" resonance="" caption="false" width="700" height="372" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 5. Surface plasmon resonance (SPR) analysis of the the Diagenode monoclonal antibody directed against 5-mC</strong><br />A synthesized biotin-labeled 5-mC conjugate was immobilized on a CM4 BIAcore sensorchip (GE Healthcare, France). Briefly, two flowcells were prepared by sequential injections of EDC/NHS, streptavidin, and ethanolamine. One of these flowcells served as negative control (biotinylated spacer without 5-mC), while biotinylated 5-mC conjugate was injected in the other one, to get an immobilization level of 55 response units (RU). All SPR experiments were performed, using HBS-N buffer (10 mM HEPES,150 mM NaCl, pH 7.4), at a flow rate of 5 µl/min. Interaction assays involved injections of 2 different dilutions of the Diagenode 5-mC monoclonal antibody (Cat. No. C15200081) over the biotinylated 5-mC conjugate and negative control surfaces, followed by a 3 min washing step with HBS-N buffer to allow dissociation of the complexes formed. At the end of each cycle, the streptavidin surface was regenerated by injection of 0.1M citric acid (pH=3).</small></p> <p><small>The sensorgrams correspond to the biotinylated 5-mC conjugate surface signal subtracted with the negative control. Data from the sensorgrams that reached binding equilibrium were used for Scatchard analysis. The value of the dissociation constant (kd) obtained by global fitting and 1:1 Langmuir model is 65 nM.</small></p> </div> </div>-->', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200081-100', 'old_catalog_number' => 'MAb-081-100', 'sf_code' => 'C15200081-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '505', 'price_USD' => '575', 'price_GBP' => '450', 'price_JPY' => '79110', 'price_CNY' => '0', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'October 27, 2020', 'slug' => '5-mc-monoclonal-antibody-33d3-premium-100-ug-50-ul', 'meta_title' => '5-methylcytosine (5-mC) Antibody - clone 33D3 (C15200081) | Diagenode', 'meta_keywords' => '5-methylcytosine (5-mC),monoclonal antibody,Methylated DNA Immunoprecipitation', 'meta_description' => '5-methylcytosine (5-mC) Monoclonal Antibody, clone 33D3 validated in MeDIP-seq, MeDIP, DB and IF. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-05-17 10:08:33', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2033', 'antibody_id' => '59', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rat) ', 'description' => '<p>5<span>-hmC is a DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig1.png" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Hydroxymethylated DNA IP results obtained with our hMeDIP kit (Cat. No. AF-104-0016)</strong><br /> Hydroxymethylated DNA IP (hMeDIP) assays were performed using the Diagenode hMeDIP kit. This kit includes: the monoclonal antibody against 5-hydroxymethylcytosine (Cat. No. MAb-633HMC-050), 5-hmC, 5-mC & cytosine DNA standards & Rat IgG (Cat. No. AF-105-0025). The DNA was prepared with the GenDNA module and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. 1 μg of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP’d material has been analysed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control • with Rat IgG as negative control (0.06%, almost no recovery) • with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control • with Rat IgG as negative control (0.03%, almost no recovery) • with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control • with Rat IgG as negative control (0.04%, almost no recovery) • with 5-hmC (97.60% recovery, almost full recovery) These results clearly demonstrate the high specificity and efficiency of the 5-hydroxymethylcytosine monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig2.png" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="375" height="274" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the 5-hmC rat monoclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody directed against 5-hmC (Cat No. MAb-633HMC-050, MAb-633HMC-100) in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig3.png" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" width="190" height="192" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dot blot analysis of the Diagenode 5-hmC and 5-mC monoclonal antibodies with the C, mC and hmC PCR controls</strong><br />Figure 3A: Approximately 200 ng, equivalent 10 pmol of C-bases, of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 5-hydroxymethylcytosine rat monoclonal antibody (dilution 1:500 ; 4 μg/ml final concentration), followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed during 30 seconds. Figure 3B: Incubation of the same membrane with the 5-methylcytosine mouse monoclonal antibody (Cat. No. MAb-335MEC-100/500) (dilution 1:250). Note that the membrane was not stripped after the 5-hmC incubation. The left spot represents the remaining hmC signal. This result confirms that an equal amount of mC bases was spotted at position 2.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig4.png" style="display: block; margin-left: auto; margin-right: auto;" width="115" height="232" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 4. Dot blot analysis of the Diagenode 5-hmC rat monoclonal antibody with the C, mC and hmC PCR controls</strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-base) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode « 5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 4 μg/ml (dilution 1:500) of the 5-hydroxymethylcytosine rat monoclonal antibody, followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15220001', 'old_catalog_number' => 'MAb-633HMC-050', 'sf_code' => 'C15220001-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-rat-classic-50-ug-32-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available', 'modified' => '2024-11-19 16:58:50', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2009', 'antibody_id' => '47', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (mouse) ', 'description' => '<p>One of the <strong>only two monoclonal antibodies raised against 5-hydroxymethylcytosine (5-hmC).</strong> 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig1.png" alt="ChIP" width="160" caption="false" height="280" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode mouse monoclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. MAb-31HMC-020, MAb-31HMC-050, MAb-31HMC-100).</strong> <br />The IgG isotype antibodies from mouse (Cat. No. kch-819-015) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the mouse monoclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig2.png" alt="ELISA" width="190" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Determination of the 5-hmC mouse monoclonal antibody titer </strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode mouse monoclonal antibody directed against 5-hmC (Cat No. MAb-31HMC-050, MAb-31HMC-100) in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig3.png" alt="Dot Blot" width="100" caption="false" height="137" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dotblot analysis of the Diagenode 5-hmC mouse monoclonal antibody with the C, mC and hmC PCR controls </strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 2 μg/ml of the mouse 5-hydroxymethylcytosine monoclonal antibody (dilution 1:500). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C15200200', 'old_catalog_number' => 'Mab-31HMC-050', 'sf_code' => 'C15200200-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-mouse-classic-50-ug-50-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:52:54', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2138', 'antibody_id' => '37', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised against 5-hydroxymethylcytosine (5-hmC). 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-elisa.png" alt="ELISA" width="342" height="266" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the 5-hmC rabbit polyclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig2.png" alt="" width="161" height="399" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode rabbit polyclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. CS-HMC-100).</strong><br />The IgG isotype antibodies from rabbit (Cat. No. kch-504-250) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the Diagenode rabbit polyclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig3.png" alt="Dot Blot" width="135" height="119" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dotblot analysis of the Diagenode 5-hmC rabbit polyclonal antibody with the C, mC and hmC PCR controls</strong><br />100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the rabbit 5-hydroxymethylcytosine polyclonal antibody (dilution 1:200). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310210-100', 'old_catalog_number' => 'CS-HMC-100', 'sf_code' => 'C15310210-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-100-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,polyclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, ELISA and DB. Batch-specific data available on the website. Sample size available', 'modified' => '2022-01-05 15:27:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '2677', 'antibody_id' => '35', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig1.jpg" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 hMeDIP results obtained with the Diagenode antibody directed against 5-hmC</strong><br /> hMeDIP (hydroxymethylated DNA IP) was performed using the Diagenode antibody against 5-hydroxymethylcytosine (Cat. No. pAb-HMC-050). DNA from mouse ES cells was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. One μg of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack for hMeDIP” (Cat No. AF-107-0040). hMeDIP was performed with 3.5 μg of the rabbit 5-hmC antibody and the IP’d DNA was analysed by qPCR using primers specific for the 3 different control sequences. Figure 1 shows that the Diagenode rabbit polyclonal antibody against 5-hmC is highly specific for the 5-hmC base modification (no IP with non-methylated or methylated C control fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig2.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Determination of the antibody titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-hmC (cat. No. pAb-HMC-050), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig3.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3 Dot blot analysis using the Diagenode antibody directed against 5-hmC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-hmC (cat. No. pAb-HMC-050), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:1,000. Figure 3 shows a high specificity of the antibody for the hydroxymethylated control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410205', 'old_catalog_number' => 'pAb-HMC-050', 'sf_code' => 'C15410205-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-50-ug', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,Polyclonal antibody,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:44:20', 'created' => '2015-07-31 14:55:13', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '2136', 'antibody_id' => '440', 'name' => '5-formylcytosine (5-fC) Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-formylcytosine (5-fC) conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-DIP.png" alt="DIP" height="433" width="400" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. DIP results obtained with the Diagenode antibody directed against 5-fC</strong><br />HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the Diagenode antibody against 5-fC (Cat. No. C15310200) in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-fig1.jpg" alt="ELISA" height="277" width="379" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-fC (Cat. No. C15310200). The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be >1:100,000.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until a few years ago, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. As such it may play a role in the regulation of gene activity. This pathway includes further oxidation of the hydroxymethyl group to a formyl or carboxyl group, both catalyzed by TET oxygenases. The formyl and carboxyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) can be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC, 5-hmC and 5-caC. Now, we also present a unique rabbit polyclonal antibody against 5-fC.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310200', 'old_catalog_number' => '', 'sf_code' => 'C15310200-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-formylcytosine-polyclonal-antibody-classic-100-ul', 'meta_title' => '5-formylcytosine (5-fC) Polyclonal Antibody | Diagenode', 'meta_keywords' => '5-formylcytosine (5-fC), polyclonal antibody,Diagenode', 'meta_description' => '5-formylcytosine (5-fC) Polyclonal Antibody validated in DIP and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-01-30 14:16:16', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 10 => array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.</div> <p><span> </span></p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Dotblot.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Fig. 1. Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC and 5-hmC. Now, we also present a unique rabbit polyclonal antibody against 5-Carboxycytosine.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410204-100', 'old_catalog_number' => 'pAb-caC-100', 'sf_code' => 'C15410204-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-cac-polyclonal-antibody-classic-100-ug', 'meta_title' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'Immunoprecipitation,5-Carboxylcytosine (5-caC),polyclonal antibody', 'meta_description' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody validated in DB, IF and IP. Batch-specific data available on the website. Sample size available', 'modified' => '2024-01-17 20:11:37', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '23', 'position' => '40', 'parent_id' => '4', 'name' => 'DNA modifications', 'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p> <p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p> <p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p> <p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p> <p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p> <p>Check out the list below to see all proposed antibodies for DNA modifications.</p> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'dna-methylation-antibody', 'cookies_tag_id' => null, 'meta_keywords' => 'Monoclonal Antibodies,Polyclonal antibodies,DNA methylation,Diagenode', 'meta_description' => 'Diagenode offers Monoclonal and Polyclonal antibodies for DNA Methylation. 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', 'meta_title' => 'DNA modifications - Monoclonal and Polyclonal Antibodies for DNA Methylation | Diagenode', 'modified' => '2020-08-03 15:25:37', 'created' => '2014-07-31 03:51:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '530', 'name' => 'Datasheet DNMT1 CS-111-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_DNMT1_CS-111-100.pdf', 'slug' => 'datasheet-dnmt1-cs-111-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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aria-hidden="true" role="dialog"> <form action="/en/carts/add/2280" id="CartAdd/2280Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2280" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 5-Carboxylcytosine (5-caC) Antibody </strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="5-cac-polyclonal-antibody-classic-100-ug" data-reveal-id="cartModal-2280" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">5-Carboxylcytosine (5-caC) Antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to 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Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2101', 'antibody_id' => '199', 'name' => 'DNMT1 Antibody ', 'description' => '<p><span>Alternative names: <strong>Dnmt</strong>, <strong>Met1</strong>, <strong>Met-1</strong>, <strong>Uim</strong>, <strong>MCMT</strong>, <strong>M.MmuI</strong> </span></p> <p><span>Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig1.jpg" alt="ELISA" height="237" width="297" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. 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Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310111', 'old_catalog_number' => 'CS-111-100', 'sf_code' => 'C15310111-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. 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Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.', 'clonality' => '', 'isotype' => '', 'lot' => 'A386-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2 (1)</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = '' $meta_description = 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ' $meta_title = 'DNMT1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2101', 'antibody_id' => '199', 'name' => 'DNMT1 Antibody ', 'description' => '<p><span>Alternative names: <strong>Dnmt</strong>, <strong>Met1</strong>, <strong>Met-1</strong>, <strong>Uim</strong>, <strong>MCMT</strong>, <strong>M.MmuI</strong> </span></p> <p><span>Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig1.jpg" alt="ELISA" height="237" width="297" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310111', 'old_catalog_number' => 'CS-111-100', 'sf_code' => 'C15310111-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'modified' => '2022-01-05 15:30:29', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '199', 'name' => 'DNMT1 polyclonal antibody', 'description' => 'DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.', 'clonality' => '', 'isotype' => '', 'lot' => 'A386-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2 (1)</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2241', 'antibody_id' => '152', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human DNMT3A (Cat. No. pAb-084-050), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:500. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-084-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/67 µl', 'catalog_number' => 'C15410084', 'old_catalog_number' => 'pAb-084-050', 'sf_code' => 'C15410084-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-67-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. ', 'modified' => '2022-01-05 15:30:56', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2242', 'antibody_id' => '153', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 92-106.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-085-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-085-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/54 µl', 'catalog_number' => 'C15410085', 'old_catalog_number' => 'pAb-085-050', 'sf_code' => 'C15410085-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-54-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in IP, WB and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:33:31', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2243', 'antibody_id' => '154', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 107-121.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-086-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:400. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-086-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/64 µl', 'catalog_number' => 'C15410086', 'old_catalog_number' => 'pAb-086-050', 'sf_code' => 'C15410086-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-64-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:31:07', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2294', 'antibody_id' => '157', 'name' => 'DNMT3B Antibody ', 'description' => '<p>Alternative names: <strong>Dnmt3b</strong>, <strong>DNA MTase HsaIIIB</strong>, <strong>M.HsaIIIB</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using 3 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_ELISA.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against DNMT3B (Cat. No. C15410218). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:220,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT3B</strong><br /> Whole cell extracts (25 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody against DNMT3B (Cat. No. C15410218) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 3. Immunofluorescence using the Diagenode antibody directed against DNMT3B</strong><br /> Human HeLa cells were stained with the Diagenode antibody against DNMT3B (Cat. No. C15410218) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3B (UniProtKB/Swiss-Prot entry Q9UBC3) catalyses the genome wide de novo methylation of CpG residues, which regulates gene expression. DNMT3B is essential for development. DNA methylation on CpG residues is coordinated with methylation of histones. Six different isoforms of DNMT3B, produced by alternative splicing, exist although isoforms 4 and 5 may not be functional due to the absence of two conserved methyltransferase motifs.</p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg/ 16 μl', 'catalog_number' => 'C15410218', 'old_catalog_number' => '', 'sf_code' => 'C15410218-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3b-polyclonal-antibody-classic-50-mg-16-ml', 'meta_title' => 'DNMT3B Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3B (DNA methyltransferase 3B) Polyclonal Antibody validated in IF, WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB', 'modified' => '2024-01-17 17:55:24', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1980', 'antibody_id' => '630', 'name' => '5-methylcytosine (5-mC) Antibody - clone 33D3', 'description' => '<p><span>Monoclonal antibody raised in mouse against </span><b>5-mC</b><span><span> </span>(</span><b>5-methylcytosine</b><span>) conjugated to ovalbumine (</span><b>33D3 clone</b><span>).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-12 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-A.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="173" /></p> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-B.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="184" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 1. MeDIP-seq with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> Genomic DNA from E14 ES cells was sheared with the Bioruptor® to generate random fragments (size range 300 to 700 bp). One µg of the fragmented DNA was ligated to Illumina adapters and the resulting DNA was used for a standard MeDIP assay, using 2 µg of the Diagenode monoclonal against 5-mC (Cat. No. C15200081). After recovery of the methylated DNA, Illumina sequencing libraries were generated and sequenced on an Illumina Genome Analyzer according to the manufacturer’s instructions. Figure 1A and 1B show Genome browser views of CA simple repeat elements with read distributions specific for 5-mC at 2 gene locations (SigleC15 and Mfsd4). Visual inspection of the peak profiles in a genome browser reveals high enrichment of CA simple repeats in affinity-enriched methylated fragments after MeDIP with the Diagenode 5-mC monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_medip.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP" caption="false" width="355" height="372" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. MeDIP results obtained with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> MeDIP (Methylated DNA immunoprecipitation) was performed on 1 µg fragmented human genomic DNA using 0.2 µg of the Diagenode monoclonal antibody against 5-mC (cat. No. C15200081) and the MagMeDIP Kit (cat. No. C02010021). The fragmented DNA was spiked with the internal controls present in the kit (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with optimized primer sets, included in the kit, specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. Figure 2 shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_Dotblot.png" alt=" 5-mC (5-methylcytosine) Antibody validated in dot blot" caption="false" width="201" height="196" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against 5-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 5-mC (cat. No. C15200081), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (cat. No. C02040010). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane. Figure 3 shows a high specificity of the antibody for the methylated control.</small></p> </div> </div> <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_IF1.png" alt="5-mC (5-methylcytosine) Antibody for immunofluorescence" height="121" width="500" caption="false" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against 5-mC</strong><br />HeLa cells were stained with the Diagenode antibody against 5-mC (Cat. No. C15200081) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p> </div> </div> <!-- <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_SPR.png" alt="5-methylcytosine (5-mC) Antibody" surface="" plasmon="" resonance="" caption="false" width="700" height="372" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 5. Surface plasmon resonance (SPR) analysis of the the Diagenode monoclonal antibody directed against 5-mC</strong><br />A synthesized biotin-labeled 5-mC conjugate was immobilized on a CM4 BIAcore sensorchip (GE Healthcare, France). Briefly, two flowcells were prepared by sequential injections of EDC/NHS, streptavidin, and ethanolamine. One of these flowcells served as negative control (biotinylated spacer without 5-mC), while biotinylated 5-mC conjugate was injected in the other one, to get an immobilization level of 55 response units (RU). All SPR experiments were performed, using HBS-N buffer (10 mM HEPES,150 mM NaCl, pH 7.4), at a flow rate of 5 µl/min. Interaction assays involved injections of 2 different dilutions of the Diagenode 5-mC monoclonal antibody (Cat. No. C15200081) over the biotinylated 5-mC conjugate and negative control surfaces, followed by a 3 min washing step with HBS-N buffer to allow dissociation of the complexes formed. At the end of each cycle, the streptavidin surface was regenerated by injection of 0.1M citric acid (pH=3).</small></p> <p><small>The sensorgrams correspond to the biotinylated 5-mC conjugate surface signal subtracted with the negative control. Data from the sensorgrams that reached binding equilibrium were used for Scatchard analysis. The value of the dissociation constant (kd) obtained by global fitting and 1:1 Langmuir model is 65 nM.</small></p> </div> </div>-->', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200081-100', 'old_catalog_number' => 'MAb-081-100', 'sf_code' => 'C15200081-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '505', 'price_USD' => '575', 'price_GBP' => '450', 'price_JPY' => '79110', 'price_CNY' => '0', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'October 27, 2020', 'slug' => '5-mc-monoclonal-antibody-33d3-premium-100-ug-50-ul', 'meta_title' => '5-methylcytosine (5-mC) Antibody - clone 33D3 (C15200081) | Diagenode', 'meta_keywords' => '5-methylcytosine (5-mC),monoclonal antibody,Methylated DNA Immunoprecipitation', 'meta_description' => '5-methylcytosine (5-mC) Monoclonal Antibody, clone 33D3 validated in MeDIP-seq, MeDIP, DB and IF. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-05-17 10:08:33', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2033', 'antibody_id' => '59', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rat) ', 'description' => '<p>5<span>-hmC is a DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig1.png" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Hydroxymethylated DNA IP results obtained with our hMeDIP kit (Cat. No. AF-104-0016)</strong><br /> Hydroxymethylated DNA IP (hMeDIP) assays were performed using the Diagenode hMeDIP kit. This kit includes: the monoclonal antibody against 5-hydroxymethylcytosine (Cat. No. MAb-633HMC-050), 5-hmC, 5-mC & cytosine DNA standards & Rat IgG (Cat. No. AF-105-0025). The DNA was prepared with the GenDNA module and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. 1 μg of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP’d material has been analysed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control • with Rat IgG as negative control (0.06%, almost no recovery) • with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control • with Rat IgG as negative control (0.03%, almost no recovery) • with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control • with Rat IgG as negative control (0.04%, almost no recovery) • with 5-hmC (97.60% recovery, almost full recovery) These results clearly demonstrate the high specificity and efficiency of the 5-hydroxymethylcytosine monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig2.png" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="375" height="274" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the 5-hmC rat monoclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody directed against 5-hmC (Cat No. MAb-633HMC-050, MAb-633HMC-100) in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig3.png" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" width="190" height="192" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dot blot analysis of the Diagenode 5-hmC and 5-mC monoclonal antibodies with the C, mC and hmC PCR controls</strong><br />Figure 3A: Approximately 200 ng, equivalent 10 pmol of C-bases, of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 5-hydroxymethylcytosine rat monoclonal antibody (dilution 1:500 ; 4 μg/ml final concentration), followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed during 30 seconds. Figure 3B: Incubation of the same membrane with the 5-methylcytosine mouse monoclonal antibody (Cat. No. MAb-335MEC-100/500) (dilution 1:250). Note that the membrane was not stripped after the 5-hmC incubation. The left spot represents the remaining hmC signal. This result confirms that an equal amount of mC bases was spotted at position 2.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig4.png" style="display: block; margin-left: auto; margin-right: auto;" width="115" height="232" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 4. Dot blot analysis of the Diagenode 5-hmC rat monoclonal antibody with the C, mC and hmC PCR controls</strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-base) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode « 5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 4 μg/ml (dilution 1:500) of the 5-hydroxymethylcytosine rat monoclonal antibody, followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15220001', 'old_catalog_number' => 'MAb-633HMC-050', 'sf_code' => 'C15220001-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-rat-classic-50-ug-32-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available', 'modified' => '2024-11-19 16:58:50', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2009', 'antibody_id' => '47', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (mouse) ', 'description' => '<p>One of the <strong>only two monoclonal antibodies raised against 5-hydroxymethylcytosine (5-hmC).</strong> 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig1.png" alt="ChIP" width="160" caption="false" height="280" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode mouse monoclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. MAb-31HMC-020, MAb-31HMC-050, MAb-31HMC-100).</strong> <br />The IgG isotype antibodies from mouse (Cat. No. kch-819-015) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the mouse monoclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig2.png" alt="ELISA" width="190" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Determination of the 5-hmC mouse monoclonal antibody titer </strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode mouse monoclonal antibody directed against 5-hmC (Cat No. MAb-31HMC-050, MAb-31HMC-100) in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig3.png" alt="Dot Blot" width="100" caption="false" height="137" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dotblot analysis of the Diagenode 5-hmC mouse monoclonal antibody with the C, mC and hmC PCR controls </strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 2 μg/ml of the mouse 5-hydroxymethylcytosine monoclonal antibody (dilution 1:500). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C15200200', 'old_catalog_number' => 'Mab-31HMC-050', 'sf_code' => 'C15200200-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-mouse-classic-50-ug-50-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:52:54', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2138', 'antibody_id' => '37', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised against 5-hydroxymethylcytosine (5-hmC). 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-elisa.png" alt="ELISA" width="342" height="266" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the 5-hmC rabbit polyclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig2.png" alt="" width="161" height="399" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode rabbit polyclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. CS-HMC-100).</strong><br />The IgG isotype antibodies from rabbit (Cat. No. kch-504-250) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the Diagenode rabbit polyclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig3.png" alt="Dot Blot" width="135" height="119" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dotblot analysis of the Diagenode 5-hmC rabbit polyclonal antibody with the C, mC and hmC PCR controls</strong><br />100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the rabbit 5-hydroxymethylcytosine polyclonal antibody (dilution 1:200). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310210-100', 'old_catalog_number' => 'CS-HMC-100', 'sf_code' => 'C15310210-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-100-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,polyclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, ELISA and DB. Batch-specific data available on the website. Sample size available', 'modified' => '2022-01-05 15:27:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '2677', 'antibody_id' => '35', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig1.jpg" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 hMeDIP results obtained with the Diagenode antibody directed against 5-hmC</strong><br /> hMeDIP (hydroxymethylated DNA IP) was performed using the Diagenode antibody against 5-hydroxymethylcytosine (Cat. No. pAb-HMC-050). DNA from mouse ES cells was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. One μg of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack for hMeDIP” (Cat No. AF-107-0040). hMeDIP was performed with 3.5 μg of the rabbit 5-hmC antibody and the IP’d DNA was analysed by qPCR using primers specific for the 3 different control sequences. Figure 1 shows that the Diagenode rabbit polyclonal antibody against 5-hmC is highly specific for the 5-hmC base modification (no IP with non-methylated or methylated C control fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig2.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Determination of the antibody titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-hmC (cat. No. pAb-HMC-050), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig3.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3 Dot blot analysis using the Diagenode antibody directed against 5-hmC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-hmC (cat. No. pAb-HMC-050), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:1,000. Figure 3 shows a high specificity of the antibody for the hydroxymethylated control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410205', 'old_catalog_number' => 'pAb-HMC-050', 'sf_code' => 'C15410205-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-50-ug', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,Polyclonal antibody,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:44:20', 'created' => '2015-07-31 14:55:13', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '2136', 'antibody_id' => '440', 'name' => '5-formylcytosine (5-fC) Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-formylcytosine (5-fC) conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-DIP.png" alt="DIP" height="433" width="400" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. DIP results obtained with the Diagenode antibody directed against 5-fC</strong><br />HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the Diagenode antibody against 5-fC (Cat. No. C15310200) in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-fig1.jpg" alt="ELISA" height="277" width="379" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-fC (Cat. No. C15310200). The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be >1:100,000.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until a few years ago, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. As such it may play a role in the regulation of gene activity. This pathway includes further oxidation of the hydroxymethyl group to a formyl or carboxyl group, both catalyzed by TET oxygenases. The formyl and carboxyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) can be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC, 5-hmC and 5-caC. Now, we also present a unique rabbit polyclonal antibody against 5-fC.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310200', 'old_catalog_number' => '', 'sf_code' => 'C15310200-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-formylcytosine-polyclonal-antibody-classic-100-ul', 'meta_title' => '5-formylcytosine (5-fC) Polyclonal Antibody | Diagenode', 'meta_keywords' => '5-formylcytosine (5-fC), polyclonal antibody,Diagenode', 'meta_description' => '5-formylcytosine (5-fC) Polyclonal Antibody validated in DIP and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-01-30 14:16:16', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 10 => array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.</div> <p><span> </span></p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Dotblot.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Fig. 1. Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC and 5-hmC. Now, we also present a unique rabbit polyclonal antibody against 5-Carboxycytosine.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410204-100', 'old_catalog_number' => 'pAb-caC-100', 'sf_code' => 'C15410204-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-cac-polyclonal-antibody-classic-100-ug', 'meta_title' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'Immunoprecipitation,5-Carboxylcytosine (5-caC),polyclonal antibody', 'meta_description' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody validated in DB, IF and IP. Batch-specific data available on the website. Sample size available', 'modified' => '2024-01-17 20:11:37', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '23', 'position' => '40', 'parent_id' => '4', 'name' => 'DNA modifications', 'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. 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Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p> <p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p> <p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p> <p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p> <p>Check out the list below to see all proposed antibodies for DNA modifications.</p> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'dna-methylation-antibody', 'cookies_tag_id' => null, 'meta_keywords' => 'Monoclonal Antibodies,Polyclonal antibodies,DNA methylation,Diagenode', 'meta_description' => 'Diagenode offers Monoclonal and Polyclonal antibodies for DNA Methylation. 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aria-hidden="true" role="dialog"> <form action="/en/carts/add/2280" id="CartAdd/2280Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2280" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 5-Carboxylcytosine (5-caC) Antibody </strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="5-cac-polyclonal-antibody-classic-100-ug" data-reveal-id="cartModal-2280" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">5-Carboxylcytosine (5-caC) Antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.</div> <p><span> </span></p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Dotblot.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Fig. 1. Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC and 5-hmC. Now, we also present a unique rabbit polyclonal antibody against 5-Carboxycytosine.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410204-100', 'old_catalog_number' => 'pAb-caC-100', 'sf_code' => 'C15410204-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-cac-polyclonal-antibody-classic-100-ug', 'meta_title' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'Immunoprecipitation,5-Carboxylcytosine (5-caC),polyclonal antibody', 'meta_description' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody validated in DB, IF and IP. Batch-specific data available on the website. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2101', 'antibody_id' => '199', 'name' => 'DNMT1 Antibody ', 'description' => '<p><span>Alternative names: <strong>Dnmt</strong>, <strong>Met1</strong>, <strong>Met-1</strong>, <strong>Uim</strong>, <strong>MCMT</strong>, <strong>M.MmuI</strong> </span></p> <p><span>Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig1.jpg" alt="ELISA" height="237" width="297" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310111', 'old_catalog_number' => 'CS-111-100', 'sf_code' => 'C15310111-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'modified' => '2022-01-05 15:30:29', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '199', 'name' => 'DNMT1 polyclonal antibody', 'description' => 'DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.', 'clonality' => '', 'isotype' => '', 'lot' => 'A386-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2 (1)</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = '' $meta_description = 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ' $meta_title = 'DNMT1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2101', 'antibody_id' => '199', 'name' => 'DNMT1 Antibody ', 'description' => '<p><span>Alternative names: <strong>Dnmt</strong>, <strong>Met1</strong>, <strong>Met-1</strong>, <strong>Uim</strong>, <strong>MCMT</strong>, <strong>M.MmuI</strong> </span></p> <p><span>Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig1.jpg" alt="ELISA" height="237" width="297" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310111', 'old_catalog_number' => 'CS-111-100', 'sf_code' => 'C15310111-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'modified' => '2022-01-05 15:30:29', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '199', 'name' => 'DNMT1 polyclonal antibody', 'description' => 'DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.', 'clonality' => '', 'isotype' => '', 'lot' => 'A386-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2 (1)</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2241', 'antibody_id' => '152', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human DNMT3A (Cat. No. pAb-084-050), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:500. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-084-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/67 µl', 'catalog_number' => 'C15410084', 'old_catalog_number' => 'pAb-084-050', 'sf_code' => 'C15410084-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-67-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. ', 'modified' => '2022-01-05 15:30:56', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2242', 'antibody_id' => '153', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 92-106.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-085-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-085-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/54 µl', 'catalog_number' => 'C15410085', 'old_catalog_number' => 'pAb-085-050', 'sf_code' => 'C15410085-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-54-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in IP, WB and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:33:31', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2243', 'antibody_id' => '154', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 107-121.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-086-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:400. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-086-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/64 µl', 'catalog_number' => 'C15410086', 'old_catalog_number' => 'pAb-086-050', 'sf_code' => 'C15410086-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-64-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:31:07', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2294', 'antibody_id' => '157', 'name' => 'DNMT3B Antibody ', 'description' => '<p>Alternative names: <strong>Dnmt3b</strong>, <strong>DNA MTase HsaIIIB</strong>, <strong>M.HsaIIIB</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using 3 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_ELISA.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against DNMT3B (Cat. No. C15410218). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:220,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT3B</strong><br /> Whole cell extracts (25 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody against DNMT3B (Cat. No. C15410218) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 3. Immunofluorescence using the Diagenode antibody directed against DNMT3B</strong><br /> Human HeLa cells were stained with the Diagenode antibody against DNMT3B (Cat. No. C15410218) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3B (UniProtKB/Swiss-Prot entry Q9UBC3) catalyses the genome wide de novo methylation of CpG residues, which regulates gene expression. DNMT3B is essential for development. DNA methylation on CpG residues is coordinated with methylation of histones. Six different isoforms of DNMT3B, produced by alternative splicing, exist although isoforms 4 and 5 may not be functional due to the absence of two conserved methyltransferase motifs.</p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg/ 16 μl', 'catalog_number' => 'C15410218', 'old_catalog_number' => '', 'sf_code' => 'C15410218-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3b-polyclonal-antibody-classic-50-mg-16-ml', 'meta_title' => 'DNMT3B Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3B (DNA methyltransferase 3B) Polyclonal Antibody validated in IF, WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB', 'modified' => '2024-01-17 17:55:24', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1980', 'antibody_id' => '630', 'name' => '5-methylcytosine (5-mC) Antibody - clone 33D3', 'description' => '<p><span>Monoclonal antibody raised in mouse against </span><b>5-mC</b><span><span> </span>(</span><b>5-methylcytosine</b><span>) conjugated to ovalbumine (</span><b>33D3 clone</b><span>).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-12 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-A.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="173" /></p> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-B.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="184" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 1. MeDIP-seq with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> Genomic DNA from E14 ES cells was sheared with the Bioruptor® to generate random fragments (size range 300 to 700 bp). One µg of the fragmented DNA was ligated to Illumina adapters and the resulting DNA was used for a standard MeDIP assay, using 2 µg of the Diagenode monoclonal against 5-mC (Cat. No. C15200081). After recovery of the methylated DNA, Illumina sequencing libraries were generated and sequenced on an Illumina Genome Analyzer according to the manufacturer’s instructions. Figure 1A and 1B show Genome browser views of CA simple repeat elements with read distributions specific for 5-mC at 2 gene locations (SigleC15 and Mfsd4). Visual inspection of the peak profiles in a genome browser reveals high enrichment of CA simple repeats in affinity-enriched methylated fragments after MeDIP with the Diagenode 5-mC monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_medip.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP" caption="false" width="355" height="372" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. MeDIP results obtained with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> MeDIP (Methylated DNA immunoprecipitation) was performed on 1 µg fragmented human genomic DNA using 0.2 µg of the Diagenode monoclonal antibody against 5-mC (cat. No. C15200081) and the MagMeDIP Kit (cat. No. C02010021). The fragmented DNA was spiked with the internal controls present in the kit (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with optimized primer sets, included in the kit, specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. Figure 2 shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_Dotblot.png" alt=" 5-mC (5-methylcytosine) Antibody validated in dot blot" caption="false" width="201" height="196" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against 5-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 5-mC (cat. No. C15200081), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (cat. No. C02040010). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane. Figure 3 shows a high specificity of the antibody for the methylated control.</small></p> </div> </div> <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_IF1.png" alt="5-mC (5-methylcytosine) Antibody for immunofluorescence" height="121" width="500" caption="false" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against 5-mC</strong><br />HeLa cells were stained with the Diagenode antibody against 5-mC (Cat. No. C15200081) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p> </div> </div> <!-- <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_SPR.png" alt="5-methylcytosine (5-mC) Antibody" surface="" plasmon="" resonance="" caption="false" width="700" height="372" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 5. Surface plasmon resonance (SPR) analysis of the the Diagenode monoclonal antibody directed against 5-mC</strong><br />A synthesized biotin-labeled 5-mC conjugate was immobilized on a CM4 BIAcore sensorchip (GE Healthcare, France). Briefly, two flowcells were prepared by sequential injections of EDC/NHS, streptavidin, and ethanolamine. One of these flowcells served as negative control (biotinylated spacer without 5-mC), while biotinylated 5-mC conjugate was injected in the other one, to get an immobilization level of 55 response units (RU). All SPR experiments were performed, using HBS-N buffer (10 mM HEPES,150 mM NaCl, pH 7.4), at a flow rate of 5 µl/min. Interaction assays involved injections of 2 different dilutions of the Diagenode 5-mC monoclonal antibody (Cat. No. C15200081) over the biotinylated 5-mC conjugate and negative control surfaces, followed by a 3 min washing step with HBS-N buffer to allow dissociation of the complexes formed. At the end of each cycle, the streptavidin surface was regenerated by injection of 0.1M citric acid (pH=3).</small></p> <p><small>The sensorgrams correspond to the biotinylated 5-mC conjugate surface signal subtracted with the negative control. Data from the sensorgrams that reached binding equilibrium were used for Scatchard analysis. The value of the dissociation constant (kd) obtained by global fitting and 1:1 Langmuir model is 65 nM.</small></p> </div> </div>-->', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200081-100', 'old_catalog_number' => 'MAb-081-100', 'sf_code' => 'C15200081-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '505', 'price_USD' => '575', 'price_GBP' => '450', 'price_JPY' => '79110', 'price_CNY' => '0', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'October 27, 2020', 'slug' => '5-mc-monoclonal-antibody-33d3-premium-100-ug-50-ul', 'meta_title' => '5-methylcytosine (5-mC) Antibody - clone 33D3 (C15200081) | Diagenode', 'meta_keywords' => '5-methylcytosine (5-mC),monoclonal antibody,Methylated DNA Immunoprecipitation', 'meta_description' => '5-methylcytosine (5-mC) Monoclonal Antibody, clone 33D3 validated in MeDIP-seq, MeDIP, DB and IF. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-05-17 10:08:33', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2033', 'antibody_id' => '59', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rat) ', 'description' => '<p>5<span>-hmC is a DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig1.png" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Hydroxymethylated DNA IP results obtained with our hMeDIP kit (Cat. No. AF-104-0016)</strong><br /> Hydroxymethylated DNA IP (hMeDIP) assays were performed using the Diagenode hMeDIP kit. This kit includes: the monoclonal antibody against 5-hydroxymethylcytosine (Cat. No. MAb-633HMC-050), 5-hmC, 5-mC & cytosine DNA standards & Rat IgG (Cat. No. AF-105-0025). The DNA was prepared with the GenDNA module and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. 1 μg of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP’d material has been analysed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control • with Rat IgG as negative control (0.06%, almost no recovery) • with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control • with Rat IgG as negative control (0.03%, almost no recovery) • with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control • with Rat IgG as negative control (0.04%, almost no recovery) • with 5-hmC (97.60% recovery, almost full recovery) These results clearly demonstrate the high specificity and efficiency of the 5-hydroxymethylcytosine monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig2.png" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="375" height="274" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the 5-hmC rat monoclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody directed against 5-hmC (Cat No. MAb-633HMC-050, MAb-633HMC-100) in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig3.png" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" width="190" height="192" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dot blot analysis of the Diagenode 5-hmC and 5-mC monoclonal antibodies with the C, mC and hmC PCR controls</strong><br />Figure 3A: Approximately 200 ng, equivalent 10 pmol of C-bases, of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 5-hydroxymethylcytosine rat monoclonal antibody (dilution 1:500 ; 4 μg/ml final concentration), followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed during 30 seconds. Figure 3B: Incubation of the same membrane with the 5-methylcytosine mouse monoclonal antibody (Cat. No. MAb-335MEC-100/500) (dilution 1:250). Note that the membrane was not stripped after the 5-hmC incubation. The left spot represents the remaining hmC signal. This result confirms that an equal amount of mC bases was spotted at position 2.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig4.png" style="display: block; margin-left: auto; margin-right: auto;" width="115" height="232" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 4. Dot blot analysis of the Diagenode 5-hmC rat monoclonal antibody with the C, mC and hmC PCR controls</strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-base) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode « 5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 4 μg/ml (dilution 1:500) of the 5-hydroxymethylcytosine rat monoclonal antibody, followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15220001', 'old_catalog_number' => 'MAb-633HMC-050', 'sf_code' => 'C15220001-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-rat-classic-50-ug-32-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available', 'modified' => '2024-11-19 16:58:50', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2009', 'antibody_id' => '47', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (mouse) ', 'description' => '<p>One of the <strong>only two monoclonal antibodies raised against 5-hydroxymethylcytosine (5-hmC).</strong> 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig1.png" alt="ChIP" width="160" caption="false" height="280" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode mouse monoclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. MAb-31HMC-020, MAb-31HMC-050, MAb-31HMC-100).</strong> <br />The IgG isotype antibodies from mouse (Cat. No. kch-819-015) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the mouse monoclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig2.png" alt="ELISA" width="190" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Determination of the 5-hmC mouse monoclonal antibody titer </strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode mouse monoclonal antibody directed against 5-hmC (Cat No. MAb-31HMC-050, MAb-31HMC-100) in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig3.png" alt="Dot Blot" width="100" caption="false" height="137" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dotblot analysis of the Diagenode 5-hmC mouse monoclonal antibody with the C, mC and hmC PCR controls </strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 2 μg/ml of the mouse 5-hydroxymethylcytosine monoclonal antibody (dilution 1:500). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C15200200', 'old_catalog_number' => 'Mab-31HMC-050', 'sf_code' => 'C15200200-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-mouse-classic-50-ug-50-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:52:54', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2138', 'antibody_id' => '37', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised against 5-hydroxymethylcytosine (5-hmC). 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-elisa.png" alt="ELISA" width="342" height="266" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the 5-hmC rabbit polyclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig2.png" alt="" width="161" height="399" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode rabbit polyclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. CS-HMC-100).</strong><br />The IgG isotype antibodies from rabbit (Cat. No. kch-504-250) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the Diagenode rabbit polyclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig3.png" alt="Dot Blot" width="135" height="119" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dotblot analysis of the Diagenode 5-hmC rabbit polyclonal antibody with the C, mC and hmC PCR controls</strong><br />100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the rabbit 5-hydroxymethylcytosine polyclonal antibody (dilution 1:200). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310210-100', 'old_catalog_number' => 'CS-HMC-100', 'sf_code' => 'C15310210-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-100-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,polyclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, ELISA and DB. Batch-specific data available on the website. Sample size available', 'modified' => '2022-01-05 15:27:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '2677', 'antibody_id' => '35', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig1.jpg" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 hMeDIP results obtained with the Diagenode antibody directed against 5-hmC</strong><br /> hMeDIP (hydroxymethylated DNA IP) was performed using the Diagenode antibody against 5-hydroxymethylcytosine (Cat. No. pAb-HMC-050). DNA from mouse ES cells was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. One μg of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack for hMeDIP” (Cat No. AF-107-0040). hMeDIP was performed with 3.5 μg of the rabbit 5-hmC antibody and the IP’d DNA was analysed by qPCR using primers specific for the 3 different control sequences. Figure 1 shows that the Diagenode rabbit polyclonal antibody against 5-hmC is highly specific for the 5-hmC base modification (no IP with non-methylated or methylated C control fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig2.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Determination of the antibody titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-hmC (cat. No. pAb-HMC-050), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig3.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3 Dot blot analysis using the Diagenode antibody directed against 5-hmC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-hmC (cat. No. pAb-HMC-050), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:1,000. Figure 3 shows a high specificity of the antibody for the hydroxymethylated control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410205', 'old_catalog_number' => 'pAb-HMC-050', 'sf_code' => 'C15410205-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-50-ug', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,Polyclonal antibody,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:44:20', 'created' => '2015-07-31 14:55:13', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '2136', 'antibody_id' => '440', 'name' => '5-formylcytosine (5-fC) Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-formylcytosine (5-fC) conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-DIP.png" alt="DIP" height="433" width="400" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. DIP results obtained with the Diagenode antibody directed against 5-fC</strong><br />HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the Diagenode antibody against 5-fC (Cat. No. C15310200) in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-fig1.jpg" alt="ELISA" height="277" width="379" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-fC (Cat. No. C15310200). The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be >1:100,000.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until a few years ago, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. As such it may play a role in the regulation of gene activity. This pathway includes further oxidation of the hydroxymethyl group to a formyl or carboxyl group, both catalyzed by TET oxygenases. The formyl and carboxyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) can be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC, 5-hmC and 5-caC. Now, we also present a unique rabbit polyclonal antibody against 5-fC.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310200', 'old_catalog_number' => '', 'sf_code' => 'C15310200-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-formylcytosine-polyclonal-antibody-classic-100-ul', 'meta_title' => '5-formylcytosine (5-fC) Polyclonal Antibody | Diagenode', 'meta_keywords' => '5-formylcytosine (5-fC), polyclonal antibody,Diagenode', 'meta_description' => '5-formylcytosine (5-fC) Polyclonal Antibody validated in DIP and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-01-30 14:16:16', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 10 => array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.</div> <p><span> </span></p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Dotblot.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Fig. 1. Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC and 5-hmC. 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Batch-specific data available on the website. Sample size available', 'modified' => '2024-01-17 20:11:37', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '23', 'position' => '40', 'parent_id' => '4', 'name' => 'DNA modifications', 'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p> <p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p> <p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p> <p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p> <p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p> <p>Check out the list below to see all proposed antibodies for DNA modifications.</p> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'dna-methylation-antibody', 'cookies_tag_id' => null, 'meta_keywords' => 'Monoclonal Antibodies,Polyclonal antibodies,DNA methylation,Diagenode', 'meta_description' => 'Diagenode offers Monoclonal and Polyclonal antibodies for DNA Methylation. 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2848', 'name' => 'DNMT1 Antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/DNMT1/SDS-C15310111-DNMT1_Antibody-US-en-GHS_1_0.pdf', 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aria-hidden="true" role="dialog"> <form action="/en/carts/add/2280" id="CartAdd/2280Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2280" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 5-Carboxylcytosine (5-caC) Antibody </strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="5-cac-polyclonal-antibody-classic-100-ug" data-reveal-id="cartModal-2280" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">5-Carboxylcytosine (5-caC) Antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.</div> <p><span> </span></p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Dotblot.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Fig. 1. Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC and 5-hmC. Now, we also present a unique rabbit polyclonal antibody against 5-Carboxycytosine.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410204-100', 'old_catalog_number' => 'pAb-caC-100', 'sf_code' => 'C15410204-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-cac-polyclonal-antibody-classic-100-ug', 'meta_title' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'Immunoprecipitation,5-Carboxylcytosine (5-caC),polyclonal antibody', 'meta_description' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody validated in DB, IF and IP. Batch-specific data available on the website. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2101', 'antibody_id' => '199', 'name' => 'DNMT1 Antibody ', 'description' => '<p><span>Alternative names: <strong>Dnmt</strong>, <strong>Met1</strong>, <strong>Met-1</strong>, <strong>Uim</strong>, <strong>MCMT</strong>, <strong>M.MmuI</strong> </span></p> <p><span>Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig1.jpg" alt="ELISA" height="237" width="297" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310111', 'old_catalog_number' => 'CS-111-100', 'sf_code' => 'C15310111-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'modified' => '2022-01-05 15:30:29', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '199', 'name' => 'DNMT1 polyclonal antibody', 'description' => 'DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.', 'clonality' => '', 'isotype' => '', 'lot' => 'A386-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2 (1)</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'en' $meta_keywords = '' $meta_description = 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ' $meta_title = 'DNMT1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2101', 'antibody_id' => '199', 'name' => 'DNMT1 Antibody ', 'description' => '<p><span>Alternative names: <strong>Dnmt</strong>, <strong>Met1</strong>, <strong>Met-1</strong>, <strong>Uim</strong>, <strong>MCMT</strong>, <strong>M.MmuI</strong> </span></p> <p><span>Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig1.jpg" alt="ELISA" height="237" width="297" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310111_fig2.jpg" alt="Western blot" height="116" width="178" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)</strong><br /> Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310111', 'old_catalog_number' => 'CS-111-100', 'sf_code' => 'C15310111-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DNMT1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT1 (DNA (cytosine-5)-methyltransferase 1) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI ', 'modified' => '2022-01-05 15:30:29', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '199', 'name' => 'DNMT1 polyclonal antibody', 'description' => 'DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.', 'clonality' => '', 'isotype' => '', 'lot' => 'A386-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1,000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2 (1)</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:56:43', 'created' => '0000-00-00 00:00:00', 'select_label' => '199 - DNMT1 polyclonal antibody (A386-004 - not determined - Mouse - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2241', 'antibody_id' => '152', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against human DNMT3A (Cat. No. pAb-084-050), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:500. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410084_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-084-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/67 µl', 'catalog_number' => 'C15410084', 'old_catalog_number' => 'pAb-084-050', 'sf_code' => 'C15410084-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-67-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. ', 'modified' => '2022-01-05 15:30:56', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2242', 'antibody_id' => '153', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 92-106.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-085-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410085_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-085-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/54 µl', 'catalog_number' => 'C15410085', 'old_catalog_number' => 'pAb-085-050', 'sf_code' => 'C15410085-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-54-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in IP, WB and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:33:31', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2243', 'antibody_id' => '154', 'name' => 'DNMT3A Antibody ', 'description' => '<p><span>Alternative names: <strong>DNMT3A2</strong>, <strong>TBRS</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 107-121.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human DNMT3A (Cat. No. pAb-086-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:400. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410086_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Western blot and Immunoprecipitation using the Diagenode antibody directed against DNMT3A</strong><br /> Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4- tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 μg of antibody against DNMT3A (Cat. No. pAb-086-050). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody (diluted 1:2,000) (lane 3). </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3A (UniProtKB/Swiss-Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/64 µl', 'catalog_number' => 'C15410086', 'old_catalog_number' => 'pAb-086-050', 'sf_code' => 'C15410086-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3a-polyclonal-antibody-classic-50-ug-64-ul', 'meta_title' => 'DNMT3A Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3A (DNA methyltransferase 3A) Polyclonal Antibody validated in WB, IP and ELISA. Batch-specific data available on the website. Alternative names: DNMT3A2, TBRS', 'modified' => '2022-01-05 15:31:07', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2294', 'antibody_id' => '157', 'name' => 'DNMT3B Antibody ', 'description' => '<p>Alternative names: <strong>Dnmt3b</strong>, <strong>DNA MTase HsaIIIB</strong>, <strong>M.HsaIIIB</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using 3 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_ELISA.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against DNMT3B (Cat. No. C15410218). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:220,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_WB.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT3B</strong><br /> Whole cell extracts (25 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody against DNMT3B (Cat. No. C15410218) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410218_IF.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 3. Immunofluorescence using the Diagenode antibody directed against DNMT3B</strong><br /> Human HeLa cells were stained with the Diagenode antibody against DNMT3B (Cat. No. C15410218) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the DNMT3B antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>DNMT3B (UniProtKB/Swiss-Prot entry Q9UBC3) catalyses the genome wide de novo methylation of CpG residues, which regulates gene expression. DNMT3B is essential for development. DNA methylation on CpG residues is coordinated with methylation of histones. Six different isoforms of DNMT3B, produced by alternative splicing, exist although isoforms 4 and 5 may not be functional due to the absence of two conserved methyltransferase motifs.</p> <p> </p>', 'label3' => '', 'info3' => '', 'format' => '50 μg/ 16 μl', 'catalog_number' => 'C15410218', 'old_catalog_number' => '', 'sf_code' => 'C15410218-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dnmt3b-polyclonal-antibody-classic-50-mg-16-ml', 'meta_title' => 'DNMT3B Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'DNMT3B (DNA methyltransferase 3B) Polyclonal Antibody validated in IF, WB and ELISA. Batch-specific data available on the website. Alternative names: Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB', 'modified' => '2024-01-17 17:55:24', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1980', 'antibody_id' => '630', 'name' => '5-methylcytosine (5-mC) Antibody - clone 33D3', 'description' => '<p><span>Monoclonal antibody raised in mouse against </span><b>5-mC</b><span><span> </span>(</span><b>5-methylcytosine</b><span>) conjugated to ovalbumine (</span><b>33D3 clone</b><span>).</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-12 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-A.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="173" /></p> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200081_ChIPSeq-B.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP-seq" caption="false" width="886" height="184" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 1. MeDIP-seq with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> Genomic DNA from E14 ES cells was sheared with the Bioruptor® to generate random fragments (size range 300 to 700 bp). One µg of the fragmented DNA was ligated to Illumina adapters and the resulting DNA was used for a standard MeDIP assay, using 2 µg of the Diagenode monoclonal against 5-mC (Cat. No. C15200081). After recovery of the methylated DNA, Illumina sequencing libraries were generated and sequenced on an Illumina Genome Analyzer according to the manufacturer’s instructions. Figure 1A and 1B show Genome browser views of CA simple repeat elements with read distributions specific for 5-mC at 2 gene locations (SigleC15 and Mfsd4). Visual inspection of the peak profiles in a genome browser reveals high enrichment of CA simple repeats in affinity-enriched methylated fragments after MeDIP with the Diagenode 5-mC monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_medip.png" alt="5-mC (5-methylcytosine) Antibody validated in MeDIP" caption="false" width="355" height="372" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. MeDIP results obtained with the Diagenode monoclonal antibody directed against 5-mC</strong><br /> MeDIP (Methylated DNA immunoprecipitation) was performed on 1 µg fragmented human genomic DNA using 0.2 µg of the Diagenode monoclonal antibody against 5-mC (cat. No. C15200081) and the MagMeDIP Kit (cat. No. C02010021). The fragmented DNA was spiked with the internal controls present in the kit (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with optimized primer sets, included in the kit, specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. Figure 2 shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200081_Dotblot.png" alt=" 5-mC (5-methylcytosine) Antibody validated in dot blot" caption="false" width="201" height="196" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against 5-mC</strong><br />To demonstrate the specificity of the Diagenode antibody against 5-mC (cat. No. C15200081), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (cat. No. C02040010). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane. Figure 3 shows a high specificity of the antibody for the methylated control.</small></p> </div> </div> <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_IF1.png" alt="5-mC (5-methylcytosine) Antibody for immunofluorescence" height="121" width="500" caption="false" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against 5-mC</strong><br />HeLa cells were stained with the Diagenode antibody against 5-mC (Cat. No. C15200081) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p> </div> </div> <!-- <div class="row"> <div class="small-12 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/C15200081_SPR.png" alt="5-methylcytosine (5-mC) Antibody" surface="" plasmon="" resonance="" caption="false" width="700" height="372" /></center></div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 5. Surface plasmon resonance (SPR) analysis of the the Diagenode monoclonal antibody directed against 5-mC</strong><br />A synthesized biotin-labeled 5-mC conjugate was immobilized on a CM4 BIAcore sensorchip (GE Healthcare, France). Briefly, two flowcells were prepared by sequential injections of EDC/NHS, streptavidin, and ethanolamine. One of these flowcells served as negative control (biotinylated spacer without 5-mC), while biotinylated 5-mC conjugate was injected in the other one, to get an immobilization level of 55 response units (RU). All SPR experiments were performed, using HBS-N buffer (10 mM HEPES,150 mM NaCl, pH 7.4), at a flow rate of 5 µl/min. Interaction assays involved injections of 2 different dilutions of the Diagenode 5-mC monoclonal antibody (Cat. No. C15200081) over the biotinylated 5-mC conjugate and negative control surfaces, followed by a 3 min washing step with HBS-N buffer to allow dissociation of the complexes formed. At the end of each cycle, the streptavidin surface was regenerated by injection of 0.1M citric acid (pH=3).</small></p> <p><small>The sensorgrams correspond to the biotinylated 5-mC conjugate surface signal subtracted with the negative control. Data from the sensorgrams that reached binding equilibrium were used for Scatchard analysis. The value of the dissociation constant (kd) obtained by global fitting and 1:1 Langmuir model is 65 nM.</small></p> </div> </div>-->', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200081-100', 'old_catalog_number' => 'MAb-081-100', 'sf_code' => 'C15200081-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '505', 'price_USD' => '575', 'price_GBP' => '450', 'price_JPY' => '79110', 'price_CNY' => '0', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'October 27, 2020', 'slug' => '5-mc-monoclonal-antibody-33d3-premium-100-ug-50-ul', 'meta_title' => '5-methylcytosine (5-mC) Antibody - clone 33D3 (C15200081) | Diagenode', 'meta_keywords' => '5-methylcytosine (5-mC),monoclonal antibody,Methylated DNA Immunoprecipitation', 'meta_description' => '5-methylcytosine (5-mC) Monoclonal Antibody, clone 33D3 validated in MeDIP-seq, MeDIP, DB and IF. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-05-17 10:08:33', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2033', 'antibody_id' => '59', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rat) ', 'description' => '<p>5<span>-hmC is a DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig1.png" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Hydroxymethylated DNA IP results obtained with our hMeDIP kit (Cat. No. AF-104-0016)</strong><br /> Hydroxymethylated DNA IP (hMeDIP) assays were performed using the Diagenode hMeDIP kit. This kit includes: the monoclonal antibody against 5-hydroxymethylcytosine (Cat. No. MAb-633HMC-050), 5-hmC, 5-mC & cytosine DNA standards & Rat IgG (Cat. No. AF-105-0025). The DNA was prepared with the GenDNA module and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. 1 μg of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP’d material has been analysed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmethylated control • with Rat IgG as negative control (0.06%, almost no recovery) • with 5-hmC antibody (0.61%, almost no recovery) - hMeDIP on methylated control • with Rat IgG as negative control (0.03%, almost no recovery) • with 5-hmC antibody (0.62%, almost no recovery) - hMeDIP on hydroxymethylated control • with Rat IgG as negative control (0.04%, almost no recovery) • with 5-hmC (97.60% recovery, almost full recovery) These results clearly demonstrate the high specificity and efficiency of the 5-hydroxymethylcytosine monoclonal antibody.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig2.png" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="375" height="274" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the 5-hmC rat monoclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody directed against 5-hmC (Cat No. MAb-633HMC-050, MAb-633HMC-100) in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig3.png" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" width="190" height="192" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dot blot analysis of the Diagenode 5-hmC and 5-mC monoclonal antibodies with the C, mC and hmC PCR controls</strong><br />Figure 3A: Approximately 200 ng, equivalent 10 pmol of C-bases, of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 5-hydroxymethylcytosine rat monoclonal antibody (dilution 1:500 ; 4 μg/ml final concentration), followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed during 30 seconds. Figure 3B: Incubation of the same membrane with the 5-methylcytosine mouse monoclonal antibody (Cat. No. MAb-335MEC-100/500) (dilution 1:250). Note that the membrane was not stripped after the 5-hmC incubation. The left spot represents the remaining hmC signal. This result confirms that an equal amount of mC bases was spotted at position 2.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15220001-fig4.png" style="display: block; margin-left: auto; margin-right: auto;" width="115" height="232" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 4. Dot blot analysis of the Diagenode 5-hmC rat monoclonal antibody with the C, mC and hmC PCR controls</strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-base) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode « 5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat. No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 4 μg/ml (dilution 1:500) of the 5-hydroxymethylcytosine rat monoclonal antibody, followed by an HRP conjugated anti-rat secondary antibody. The membrane was exposed for 30 seconds.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg', 'catalog_number' => 'C15220001', 'old_catalog_number' => 'MAb-633HMC-050', 'sf_code' => 'C15220001-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-rat-classic-50-ug-32-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (rat) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available', 'modified' => '2024-11-19 16:58:50', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2009', 'antibody_id' => '47', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (mouse) ', 'description' => '<p>One of the <strong>only two monoclonal antibodies raised against 5-hydroxymethylcytosine (5-hmC).</strong> 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig1.png" alt="ChIP" width="160" caption="false" height="280" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode mouse monoclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. MAb-31HMC-020, MAb-31HMC-050, MAb-31HMC-100).</strong> <br />The IgG isotype antibodies from mouse (Cat. No. kch-819-015) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the mouse monoclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig2.png" alt="ELISA" width="190" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Determination of the 5-hmC mouse monoclonal antibody titer </strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode mouse monoclonal antibody directed against 5-hmC (Cat No. MAb-31HMC-050, MAb-31HMC-100) in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200200-fig3.png" alt="Dot Blot" width="100" caption="false" height="137" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dotblot analysis of the Diagenode 5-hmC mouse monoclonal antibody with the C, mC and hmC PCR controls </strong><br />200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0020) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with 2 μg/ml of the mouse 5-hydroxymethylcytosine monoclonal antibody (dilution 1:500). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C15200200', 'old_catalog_number' => 'Mab-31HMC-050', 'sf_code' => 'C15200200-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-monoclonal-antibody-mouse-classic-50-ug-50-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,monoclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Monoclonal Antibody (mouse) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:52:54', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2138', 'antibody_id' => '37', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised against 5-hydroxymethylcytosine (5-hmC). 5-hmC is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Preliminary results indicate that 5-hmC may have important roles distinct from 5-methylcytosine (5-mC). Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-elisa.png" alt="ELISA" width="342" height="266" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the 5-hmC rabbit polyclonal antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig2.png" alt="" width="161" height="399" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. An hydroxymethylated DNA IP (hMeDIP) was performed using the Diagenode rabbit polyclonal antibody directed against 5-hydroxymethylcytosine (Cat. No. CS-HMC-100).</strong><br />The IgG isotype antibodies from rabbit (Cat. No. kch-504-250) was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to have DNA fragments of 300-500 bp. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP’d material has been analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the Diagenode rabbit polyclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310210-fig3.png" alt="Dot Blot" width="135" height="119" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Dotblot analysis of the Diagenode 5-hmC rabbit polyclonal antibody with the C, mC and hmC PCR controls</strong><br />100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002) were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the rabbit 5-hydroxymethylcytosine polyclonal antibody (dilution 1:200). The membranes were exposed for 30 seconds. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310210-100', 'old_catalog_number' => 'CS-HMC-100', 'sf_code' => 'C15310210-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-100-ul', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,5-hmC, 5-mC,polyclonal antibody ,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, ELISA and DB. Batch-specific data available on the website. Sample size available', 'modified' => '2022-01-05 15:27:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '2677', 'antibody_id' => '35', 'name' => '5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig1.jpg" alt="hMeDIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1 hMeDIP results obtained with the Diagenode antibody directed against 5-hmC</strong><br /> hMeDIP (hydroxymethylated DNA IP) was performed using the Diagenode antibody against 5-hydroxymethylcytosine (Cat. No. pAb-HMC-050). DNA from mouse ES cells was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. One μg of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack for hMeDIP” (Cat No. AF-107-0040). hMeDIP was performed with 3.5 μg of the rabbit 5-hmC antibody and the IP’d DNA was analysed by qPCR using primers specific for the 3 different control sequences. Figure 1 shows that the Diagenode rabbit polyclonal antibody against 5-hmC is highly specific for the 5-hmC base modification (no IP with non-methylated or methylated C control fragments). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig2.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2 Determination of the antibody titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-hmC (cat. No. pAb-HMC-050), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410205-fig3.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3 Dot blot analysis using the Diagenode antibody directed against 5-hmC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-hmC (cat. No. pAb-HMC-050), a Dot blot analysis was performed using the hmC, mC and C controls from the Diagenode “5-hmC, 5-mC & cytosine DNA Standard Pack” (Cat No. AF-101-0002). One hundred to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:1,000. Figure 3 shows a high specificity of the antibody for the hydroxymethylated control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells).</p> <p>Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics : 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies.</p> <p>Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique monoclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.</p>', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410205', 'old_catalog_number' => 'pAb-HMC-050', 'sf_code' => 'C15410205-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-hmc-polyclonal-antibody-rabbit-classic-50-ug', 'meta_title' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody(rabbit) | Diagenode', 'meta_keywords' => '5-hydroxymethylcytosine,Polyclonal antibody,Diagenode', 'meta_description' => '5-hydroxymethylcytosine (5-hmC) Polyclonal Antibody (rabbit) validated in hMeDIP, DB and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2024-11-19 16:44:20', 'created' => '2015-07-31 14:55:13', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '2136', 'antibody_id' => '440', 'name' => '5-formylcytosine (5-fC) Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against 5-formylcytosine (5-fC) conjugated to KLH.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-DIP.png" alt="DIP" height="433" width="400" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. DIP results obtained with the Diagenode antibody directed against 5-fC</strong><br />HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the Diagenode antibody against 5-fC (Cat. No. C15310200) in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310200-fig1.jpg" alt="ELISA" height="277" width="379" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-fC (Cat. No. C15310200). The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be >1:100,000.</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until a few years ago, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. As such it may play a role in the regulation of gene activity. This pathway includes further oxidation of the hydroxymethyl group to a formyl or carboxyl group, both catalyzed by TET oxygenases. The formyl and carboxyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) can be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC, 5-hmC and 5-caC. Now, we also present a unique rabbit polyclonal antibody against 5-fC.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310200', 'old_catalog_number' => '', 'sf_code' => 'C15310200-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-formylcytosine-polyclonal-antibody-classic-100-ul', 'meta_title' => '5-formylcytosine (5-fC) Polyclonal Antibody | Diagenode', 'meta_keywords' => '5-formylcytosine (5-fC), polyclonal antibody,Diagenode', 'meta_description' => '5-formylcytosine (5-fC) Polyclonal Antibody validated in DIP and ELISA. Batch-specific data available on the website. Sample size available.', 'modified' => '2023-01-30 14:16:16', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 10 => array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.</div> <p><span> </span></p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Dotblot.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Fig. 1. Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC and 5-hmC. 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Batch-specific data available on the website. Sample size available', 'modified' => '2024-01-17 20:11:37', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '23', 'position' => '40', 'parent_id' => '4', 'name' => 'DNA modifications', 'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p> <p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p> <p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p> <p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p> <p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p> <p>Check out the list below to see all proposed antibodies for DNA modifications.</p> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'dna-methylation-antibody', 'cookies_tag_id' => null, 'meta_keywords' => 'Monoclonal Antibodies,Polyclonal antibodies,DNA methylation,Diagenode', 'meta_description' => 'Diagenode offers Monoclonal and Polyclonal antibodies for DNA Methylation. 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2848', 'name' => 'DNMT1 Antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/DNMT1/SDS-C15310111-DNMT1_Antibody-US-en-GHS_1_0.pdf', 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aria-hidden="true" role="dialog"> <form action="/en/carts/add/2280" id="CartAdd/2280Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2280" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 5-Carboxylcytosine (5-caC) Antibody </strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('5-Carboxylcytosine (5-caC) Antibody ', 'C15410204-100', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="5-cac-polyclonal-antibody-classic-100-ug" data-reveal-id="cartModal-2280" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">5-Carboxylcytosine (5-caC) Antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2280', 'antibody_id' => '234', 'name' => '5-Carboxylcytosine (5-caC) Antibody ', 'description' => '<div data-canvas-width="124.25999999999996" style="left: 329.401px; top: 425.793px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.0021);">Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.</div> <p><span> </span></p> <p><strong></strong></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Dotblot.jpg" alt="Dot blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small><strong> Fig. 1. Dot blot analysis using the Diagenode antibody directed against 5-caC</strong><br /> To demonstrate the specificity of the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified C-bases (indicated in red). 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The antibody was used at a dilution of 1:1,000. The binding of antibody to the DNA was measured by ECL chemiluminescence. Figure 1 shows a high specificity of the antibody for the carboxylated cytosine. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-Immunostaining.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 2. Immunofluorescence assay using the Diagenode antibody directed against 5-caC</strong><br /> 293T cells were transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) and stained with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050), diluted 1:500, and with an anti-FLAG antibody, followed by DAPI counterstaining. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410204-chip.jpg" alt="Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Fig. 3. Immunoprecipitation using the Diagenode antibody directed against 5-caC</strong><br /> Immunoprecipitation was performed with the Diagenode antibody against 5-caC (cat. No. pAb-CaC-020/050) on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control. Figure 3 shows the enrichment calculated as the ratio of the recovery of the control DNA versus the recovery of the GAPDH negative control. </small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<p>Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.</p> <p>Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.</p> <p>Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC and 5-hmC. Now, we also present a unique rabbit polyclonal antibody against 5-Carboxycytosine.</p>', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15410204-100', 'old_catalog_number' => 'pAb-caC-100', 'sf_code' => 'C15410204-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '5-cac-polyclonal-antibody-classic-100-ug', 'meta_title' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody | Diagenode', 'meta_keywords' => 'Immunoprecipitation,5-Carboxylcytosine (5-caC),polyclonal antibody', 'meta_description' => '5-Carboxylcytosine (5-caC) Polyclonal Antibody validated in DB, IF and IP. Batch-specific data available on the website. 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