Alternative names: Dnmt3b, DNA MTase HsaIIIB, M.HsaIIIB
Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.
| Lot | A357-001 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:500 | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT3B (cat# CS-044-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,500.
Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT3B
Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against DNMT3B (cat# CS-044-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left.
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