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Total RNA-seq Service

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  •  Description
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Catalog Number
Format
G02030000
Custom

Exploring the whole transcriptome allows to gain insight of potential transcriptional and regulatory network mechanisms. With Total RNA-seq services, we will sequence the whole transcriptome and record the complete set of RNA transcripts, including mRNAs and long non-coding RNAs, present in a biological sample under specific circumstances. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.

Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation can be performed with an optional rRNA depletion step to enable cost-effective sequencing of RNA samples.

High discovery power with total RNA-seq

  • Detection of both coding and non-coding RNAs with optional rRNA depletion
  • Recognition of long coding RNAs involved in chromatin remodeling and transcriptional/post-transcriptional regulation
  • Investigation of the structure of genes, their splicing patterns and other post transcriptional modifications
  • Suitable for a broad range of input amounts
  • Compatible with FFPE samples
  • Fast turnaround time (from 5 to 6 weeks)

See our other RNA-seq Profiling Services

  • Sample Requirements
    • Project size: minimum of 24 samples
    • Sample type: total isolated RNA
    • Required quantities: minimum 5 ng of total RNA (Human, Mouse, Rat) or 100 ng (Plant)
    • Shipment conditions: shipped in 1.5 mL or 0.5 mL tubes on dry ice
  • Services Workflow
    Total RNA-seq Service

    What our service includes

    1. RNA quality control
      • Determination of RIN value (RNA integration number)
      • Measurement of RNA concentration
    2. Library preparation
      • Optional rRNA depletion
      • Stranded library construction
      • Quality control of libraries prior to sequencing
    3. Sequencing on Illumina platforms
      • 50 million raw reads per sample
    4. Data analysis (optional for additional fee)
      • Standard bioinformatic analysis
      • Advanced bioinformatic analysis
      • Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
  • Bioinformatics Analysis

    Standard bioinformatic analysis
    (optional for additional fee)

    Advanced bioinformatic analysis
    (optional for additional fee)
    • Demultiplexing, read quality control, trimming and filtering
    • Alignment to reference genome
    • Quantification of coding and non-coding RNA biotypes with specialized quantification tool MGCount (software developed by Diagenode)

    Provided files:

    • Report with sequencing statistics
    • Raw data in FASTQ format
    • FastQC report
    • Alignment in BAM format
    • Un-normalized read counts
    • Differential expression analysis
    • Gene ontology terms analysis
    • Pathway analysis
    • Alternative splicing analysis
    • Novel transcript identification

  •  Documents
    The Diagenode Epigenetics custom service POSTER
    Complete workflows for genome-scale DNA methylation and histone marks analysis Epigenetics is cr...    		 Download
  •  Publications

    How to properly cite our product/service in your work

    We strongly recommend using this: Total RNA-seq Service (Hologic Diagenode Cat# G02030000). Click here to copy to clipboard.

    Using our products or services in your publication? Let us know!

    Long-term effects of myo-inositol on traumatic brain injury: Epigenomic and transcriptomic studies
    Oganezovi N. et al.
    Background and purpose Traumatic brain injury (TBI) and its consequences remain great challenges for neurology. Consequences of TBI are associated with various alterations in the brain but little is known about long-term changes of epigenetic DNA methylation patterns. Moreover, nothing is known about potential trea...

    Inflammatory stress-mediated chromatin changes underlie dysfunction in endothelial cells
    Liu H. et al.
    Inflammatory stresses underlie endothelial dysfunction and contribute to the development of chronic cardiovascular disorders such as atherosclerosis and vascular fibrosis. The initial transcriptional response of endothelial cells to pro-inflammatory cytokines such as TNF-alpha is well established. However, very few ...

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