TET3 Polyclonal Antibody

Lot	A2307P
Concentration	1 µg/µl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:1,000	Fig 1
Western Blotting	1:1,000	Fig 2, 3

ELISA

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against mouse TET3 (cat. No. C15410311). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:20,300.

Western blot

Figure 2. Western blot analysis using the Diagenode antibody directed against TET3
Whole cell extracts (25 μg) from Jurkat cells were analysed by Western blot using the Diagenode antibody against TET3 (cat. No. C15410311) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Western blot

Figure 3. Western blot analysis using the Diagenode antibody directed against TET3
Whole cell extracts (25 μg) from Jurkat cells were analysed by Western blot using the Diagenode antibody against TET3 (cat. No. C15410311) diluted 1:200 in TBS- Tween containing 5% skimmed milk. Lane 2 shows the results after incubation of the antibody with the immunizing peptides. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Target description

TET3 (UniProtKB/Swiss-Prot entry O43151) is a member of the ten-eleven translocation (TET) gene family which play a role in the DNA methylation process. It catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which is the first step in demethylation of the DNA. TET3 may therefore play an important role in gene activation and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Diseases associated with TET3 include acute myeloid leukemia.

Applications

ELISA
Enzyme-linked immunosorbent assay. Read more

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

Documents

Datasheet TET3 C15410311 DATASHEET Polyclonal antibody raised in rabbit against TET3 (Tet Methylcytosine Dioxygenase 3), using 4 KLH...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download

Safety sheets

TET3 Antibody SDS US en	Download
TET3 Antibody SDS GB en	Download
TET3 Antibody SDS ES es	Download
TET3 Antibody SDS DE de	Download
TET3 Antibody SDS JP ja	Download
TET3 Antibody SDS BE nl	Download
TET3 Antibody SDS BE fr	Download
TET3 Antibody SDS FR fr	Download

Publications

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TET3 Antibody

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TET2 Antibody

5-methylcytosine (5-mC) Antibody - clone 33D3

5-hydroxymethylcytosine (5-hmC) Antibody (rat)

5-hydroxymethylcytosine (5-hmC) Antibody (mouse)

5-hydroxymethylcytosine (5-hmC) Antibody (rabbit)

5-hydroxymethylcytosine (5-hmC) Antibody (rabbit)

5-formylcytosine (5-fC) Antibody

5-Carboxylcytosine (5-caC) Antibody

Events