Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.
DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.
Overview of the three main approaches for studying DNA methylation.
Description | Features | |
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Bisulfite conversion | Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution. |
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Methylated DNA enrichment | (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA. |
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Restriction enzyme-based digestion | Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites. |
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Cat. No. | Product | Format | Price | |
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C02010031 |
hMeDIP kit x16 (monoclonal mouse antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto... |
16 rxns | $690.00 | |
C02010021 |
MagMeDIP qPCR Kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo... |
48 rxns (IP) | $750.00 | |
C02020010 |
MethylCap kit The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methylbinding domain) protein . This procedure has first been optimi... |
48 rxns | $695.00 | |
C02030030 |
Premium Bisulfite kit Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r... |
50 rxns | $240.00 | |
C02030036 |
Premium RRBS kit V2 (Coming soon) Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, targeted solution to perform genome-scale DNA methylation analysis... |
24 rxns | $1,590.00 | |
C02030037 |
Premium RRBS kit V2 x96 RRBS for low DNA amounts and accurate analysis Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s... |
96 rxns | $3,745.00 | |
C02010034 |
Auto hMeDIP kit x16 (monoclonal mouse antibody) This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy... |
16 rxns | $690.00 | |
C02030031 |
Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate... |
40 rxns | $240.00 | |
C02030035 |
Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S... |
8 rxns | $120.00 | |
C02040018 |
DNA hydroxymethylation control package The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer pair. The control can be ad... |
48 rxns | $150.00 | |
C02040012 |
DNA Methylation control package The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your... |
40 rxns | $300.00 | |
C02040019 |
DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes... |
48 rxns | $275.00 | |
G02180000 |
Human Methylome Service NEW! Human Methylome enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker... |
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C02020012 |
MethylCap protein The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs. It consists of the methyl binding domain (MBD) of human MeC... |
100 µg | $565.00 | |
C09010012 |
MethylTaq Plus 2X Master Mix MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfiteconvertedNGS libraries, except p... |
135 μL | $120.00 | |
C02030042 |
Premium Methyl UDI-UMI Adapters Module - 96 rxns Premium Methyl UDI-UMI Adapters Module - 96 rxns includes 96 methylated full-length adapters with unique dual indexes (UDI) and uniq... |
96 rxns | $950.00 | |
C02030040 |
Premium Methyl UMI-UDI Adapters - Set A (Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ... |
24 rxns | $250.00 | |
C02030041 |
Premium Methyl UMI-UDI Adapters - Set B (Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ... |
24 rxns | $250.00 |