Diagenode

DNA Methylation

Complete solutions for DNA methylation studies

Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.

DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.



  • Learn more
    5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.


Main DNA methylation technologies

Overview of the three main approaches for studying DNA methylation.

  1. Chemical modification with bisulfite – Bisulfite conversion
  2. Enrichment of methylated DNA (including MeDIP and MBD)
  3. Treatment with methylation-sensitive or dependent restriction enzymes

 

Description Features
Bisulfite conversion Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.
  • Single nucleotide resolution
  • Quantitative analysis - methylation rate (%)
  • Gold standard and well studied
  • Compatible with automation
Methylated DNA enrichment (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.
  • Resolution depends on the fragment size of the enriched methylated DNA (300 bp)
  • Qualitative analysis
  • Compatible with automation
Restriction enzyme-based digestion Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.
  • Determination of methylation status is limited by the enzyme recognition site
  • Easy to use

Recommended products for DNA Methylation

Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysi...
16 rxns $690.00
C02010021 MagMeDIP Kit
  Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries f...
48 rxns (IP) $750.00
C02020010 MethylCap kit
The MethylCap kit allows to specifically capture DNA fragments containing methylated CpGs. The assay is based on the affinity purification of methylated DNA ...
48 rxns
C02030030 Premium Bisulfite kit
Make your Bisulfite conversion now in only 60 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion...
50 rxns $240.00
C02030036 Premium RRBS kit V2
RRBS for low DNA amounts and accurate analysis

Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at t...
24 rxns $1,590.00
C02030037 Premium RRBS kit V2 x96
RRBS for low DNA amounts and accurate analysis

Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s...
96 rxns $3,745.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation anal...
16 rxns $690.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $240.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $120.00
C02040018 DNA hydroxymethylation control package
The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer set that can be added to the DNA...
48 rxns $150.00
C02040012 DNA methylation control package
The DNA methylation control package includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer ...
40 rxns $300.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets th...
48 rxns $275.00
G02180000 Human Methylome Service
NEW! The Human Methylome is a Next-Generation Sequencing (NGS) service assay that allows a comprehensive DNA methylation profiling in human samples from...
C02020012 MethylCap protein
The MethylCap protein has been extensively validated for specific isolation of DNA fragments containing methylated CpGs. It consists of the methyl-CpG-bindin...
100 µg $565.00
C09010012 MethylTaq Plus 2X Master Mix
MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfite converted NGS libraries, e...
135 μL $120.00
C02030042 Premium Methyl UDI-UMI Adapters Module - 96 rxns
Premium Methyl UDI-UMI Adapters Module - 96 rxns includes 96 methylated full-length adapters with unique dual indexes (UDI) and un...
96 rxns $950.00
C02030040 Premium Methyl UDI-UMI Adapters Module - Set A
Premium Methyl UDI-UMI Adapters Module - Set A includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mo...
24 rxns $250.00
C02030041 Premium Methyl UDI-UMI Adapters Module - Set B
Premium Methyl UDI-UMI Adapters Module - Set B includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mo...
24 rxns $250.00

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