Diagenode

Targeted DNA methylation service

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  •  描述
  •  文档
  •  相关
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目录号
格式
G02070000

Targeted DNA methylation analysis allows for the quantitative study of cytosine methylation at known and precise locations throughout the genome. Depending on the size of the region of interest, a number of methodologies can be employed including bisulfite pyrosequencing, bisulfite amplicon sequencing (BSAS), and custom capturing with probes. These techniques share a common methodology of bisulfite conversion followed by sequencing in order to quantitatively detect the methylation status of the target regions at single nucleotide resolution.

  • Description

  • Bioinformatic analysis

    Analysis

    Features

    Standard
    • FASTQ raw data
    • FASTQC quality control insights
    • Alignment of methyl-seq data against reference genome
    • Methylation calling and extraction
    • Summary statistics
    Differential methylation analysis
    • Methylation level analysis
    • Differentially Methylated CpGs (DMCs) analysis
    • Differentially Methylated Regions (DMRs) analysis
    • Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)

  •  出版物

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