It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.
| Chromatin Shearing Kit Low SDS (for Histone) | Chromatin Shearing Kit Low SDS (for TF) | Chromatin Shearing Kit High SDS | Chromatin Shearing Kit (for Plant) | |
|
SDS concentration |
< 0.1% |
0.2% |
1% |
0.5% |
|
Nuclei isolation |
Yes |
Yes |
No |
Yes |
|
Allows for shearing of... cells/tissue |
100 million cells |
100 million cells |
100 million cells |
up to 25 g of tissue |
|
Corresponding to shearing buffers from |
Table comes from our Guide for successful chromatin preparation using the Bioruptor® Pico
| Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c... | Download |
| Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro... | Download |
| ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus A review of the critical points for chromatin shearing. | Download |
