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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
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<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
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'info3' => '<h3>Shearing Accessories</h3>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
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<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
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<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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'name' => '15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
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'label1' => '',
'info1' => '',
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'slug' => '15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack',
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'meta_keywords' => '',
'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus',
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'name' => '50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'description' => '<p><span>The attachment for 50 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 3 x 50 ml tubes at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
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'search_order' => '01-Accessory',
'price_EUR' => '1850',
'price_USD' => '1850',
'price_GBP' => '1600',
'price_JPY' => '289800',
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'price_AUD' => '4625',
'country' => 'ALL',
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'last_datasheet_update' => '0000-00-00',
'slug' => '50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack',
'meta_title' => '50 ml tube holder for Bioruptor Standard & Bioruptor Plus',
'meta_keywords' => '',
'meta_description' => '50 ml tube holder for Bioruptor Standard & Bioruptor Plus',
'modified' => '2017-08-19 10:37:17',
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(int) 3 => array(
'id' => '1814',
'antibody_id' => null,
'name' => 'Water cooler, 115V',
'description' => '<p><span>This water cooling system allows continuous (Bioruptor® Standard or Plus) or regulated cooling (Bioruptor® Plus or Pico) of the Bioruptor sonication bath. The regulated valve (</span><a href="http://www.diagenode.com/en/catalog/sonication-54/bioruptor-accessories-56/water-cooler-accessories-60/product/single-cycle-valve-bioruptor-pico-bioruptor-plus--369" target="_blank">Single Cycle valve</a><span>) ensures that water will only be replaced during the OFF cycle to avoid any interference between the water flow and the sonication process.</span></p>',
'label1' => 'Characteristics',
'info1' => '<table>
<tbody>
<tr>
<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
</tr>
<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
</tr>
<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
</tr>
<tr>
<td>Safety classification</td>
<td>Class I/NFL</td>
</tr>
<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
</tr>
<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
</tr>
<tr>
<td>Refrigerant</td>
<td>R290</td>
</tr>
<tr>
<td>Refrigerant quantity</td>
<td>0.04 kg</td>
</tr>
<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
</tr>
<tr>
<td>max. delivery</td>
<td>20 l/min</td>
</tr>
<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
</tr>
<tr>
<td>max. delivery (suction)</td>
<td>17 l/min</td>
</tr>
<tr>
<td>Pump connection</td>
<td>G 1/4" female thread</td>
</tr>
<tr>
<td>min. filling capacity</td>
<td>1.4 l</td>
</tr>
<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
</tr>
<tr>
<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
</tr>
<tr>
<td>Net weight</td>
<td>23 kg</td>
</tr>
<tr>
<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
</tr>
<tr>
<td>max. current</td>
<td>2.8 A</td>
</tr>
<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
</tr>
<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
</tr>
<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
</tr>
</tbody>
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'name' => 'Water cooler, 230V',
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'label1' => 'Characteristics',
'info1' => '<table>
<tbody>
<tr>
<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
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<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
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<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
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<tr>
<td>Safety classification</td>
<td>Class I/NFL</td>
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<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
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<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
</tr>
<tr>
<td>Refrigerant</td>
<td>R290</td>
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<td>Refrigerant quantity</td>
<td>0.04 kg</td>
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<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
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<tr>
<td>max. delivery</td>
<td>20 l/min</td>
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<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
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<td>max. delivery (suction)</td>
<td>17 l/min</td>
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<td>Pump connection</td>
<td>G 1/4" female thread</td>
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<td>min. filling capacity</td>
<td>1.4 l</td>
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<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
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<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
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<td>Net weight</td>
<td>23 kg</td>
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<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
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<tr>
<td>max. current</td>
<td>2.8 A</td>
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<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
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<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
</tr>
<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
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'slug' => 'water-cooler-230v-1-unit',
'meta_title' => 'Water cooler, 230V',
'meta_keywords' => '',
'meta_description' => 'Water cooler, 230V',
'modified' => '2018-02-14 16:26:52',
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(int) 5 => array(
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'name' => 'Single Cycle Valve for Water Cooler',
'description' => '<p><span>The Single Cycle Valve for the Bioruptor® controls the water flow between the Bioruptor® Pico (B01060001) / Bioruptor® Plus (UCD-300) and the water cooler. Cold water cycles into the Bioruptor® sonication bath only during the off cycle (no sonication) and is stopped during the on cycle (sonication). This reduces any water flow disturbance in the tank and allows for more efficient sonication of samples.</span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '特徴',
'info1' => '<ul>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 unit',
'catalog_number' => 'B02020005',
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'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
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<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
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<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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<p><span>Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</span></p>
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<h2 style="text-align: center;">Technology explained</h2>
<div class="container-wrapper-genially" style="position: relative; min-height: 400px; max-width: 80%; margin: 0 auto;"><video width="300" height="150" style="position: absolute; top: 45%; left: 50%; transform: translate(-50%, -50%); width: 80px; height: 80px; margin-bottom: 10%;" class="loader-genially" autoplay="autoplay" loop="loop" playsinline="playsInline" muted="muted"><source src="https://static.genial.ly/resources/panel-loader-low.mp4" type="video/mp4" />Your browser does not support the video tag.</video>
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<p><span> <br /></span></p>
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<h2 style="text-align: center;">Reproductibility is our priority</h2>
</div>
</div>
<div><img src="https://www.diagenode.com/img/shearing/reproductibility.png" alt="reproductibility" />
<p class="bottom_note"></p>
</div>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;">An affordable instrument for wide range of applications</h2>
</div>
</div>
<p style="text-align: center;">Designed for any researchers, the Bioruptor gives the user the right level of flexibility.</p>
<table style="width: 972px;">
<tbody>
<tr style="height: 56px;">
<th style="width: 380px; height: 56px;"></th>
<th class="text-center" style="width: 126px; height: 56px;">Bioruptor</th>
<th class="text-center" style="width: 141px; height: 56px;">Cup Horn Sonicators</th>
<th class="text-center" style="width: 156px; height: 56px;">Focused <br />ultra-sonicators</th>
<th class="text-center" style="width: 155px; height: 56px;">Multi Sample Sonicator</th>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Instrument pricing</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Consumables pricing</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Range of applications</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Scalable and sample volume flexibility</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Throughput</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
</tbody>
</table>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">Bioruptor ultrasonication for best results in:</h2>
<p><b><span>✓ Chromatin shearing</span><span> </span><span style="font-weight: 400;">- Industry leader in accurate and tight fragment ranges</span></b></p>
<p><b><span>✓ DNA shearing</span><span> </span><span style="font-weight: 400;">- Excellent results for optimal fragment lengths in NGS library prep</span></b></p>
<p><b><span>✓<span> </span></span>Protein aggregation studies </b><span style="font-weight: 400;">- Standardizing seeding with the robust Bioruptor.<br /></span><i><span style="font-weight: 400;">Read the app note by Dr. Kelvin Luk at the University of Pennsylvania </span></i><a href="https://www.diagenode.com/en/documents/standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease" style="color: #13b29c;"><i><span style="font-weight: 400;">“Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>3D genome analysis with Hi-C</b><span style="font-weight: 400;"> - Preparing chromatin libraries with high-quality sonication.<br /></span><i><span style="font-weight: 400;">Read the app note, “</span></i><a href="https://www.diagenode.com/en/documents/applicationnote-arima-low-input" style="color: #13b29c;"><i><span style="font-weight: 400;">Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>Mass spectrometry</b> <b>and increasing protein identification</b><span style="font-weight: 400;">- Sample preparation using Preomics iST and Bioruptor sonication.<br /></span><i><span style="font-weight: 400;">Read the app note “</span></i><a href="https://www.diagenode.com/en/documents/wp-ist-adaptators" style="color: #13b29c;"><i><span style="font-weight: 400;">Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder”</span></i></a></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>Cell lysis, liposome prep, protein extraction, RNA extraction and more</b></span></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>CUT&RUN –Sonication of input DNA (for enrichment comparison) for NGS</b></span></p>
</div>
</div>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
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<p><span>Disruption of bacterial cells is required for releasing biological molecules from within the cells for various biological applications. Ultrasound technology has been widely used for such cell lysis applications. Ultrasonic disruption is based on cavitation, the creation of cavities in cell suspension. These cavities or small bubbles of dissolved gases or vapors arise from the alteration of pressure in liquids. The bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming leading to mechanical stress that effectively lyses cells. Diagenode’s Bioruptor</span><span><sup>®</sup> </span><span>sonicaton device uses state-of-the-art ultrasound technology to disrupt cells for biological, chemical, pharmaceutical, and industrial applications. </span></p>
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'id' => '42',
'name' => 'Bioruptor<sup>®</sup> NGS: Unbiased DNA shearing for Next-Generation Sequencing',
'description' => '<p><span>Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center, University of Liège (Belgium). The center routinely performs a variety of Next-Generation Sequencing (NGS) applications including de novo sequencing, whole genome and amplicon sequencing, and targeted resequencing on an Illumina GAII</span><span>x </span><span>analyzer. Coppieters recognizes that optimal sequencing results rely heavily on the quality and preparation of DNA libraries. Library preparation can be complex with multiple steps that require optimization. As Coppieters notes, “A prerequisite to obtaining high quality Next-Generation Sequencing data is to have libraries that have the desired insert size (November 2011)”. </span></p>',
'image_id' => null,
'type' => 'Case study',
'url' => 'files/case_studies/CaseStudy-Bioruptor.pdf',
'slug' => 'casestudy-bioruptor',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 15:45:29',
'created' => '2015-07-03 16:05:36',
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(int) 4 => array(
'id' => '964',
'name' => 'LC-MS measurement of whole-body uptake of pharmaceuticals in zebrafish using the Bioruptor® Plus for sample preparation',
'description' => '<p>The laboratory for Pharmaceutical Analysis at KU Leuven is developing innovative separation and detection techniques in pharmaceutical applications. Within the Department of Pharmaceutical and Pharmacological Sciences, the research group of Professor Deirdre Cabooter is specifically working on the implementation of different liquid chromatography (LC) based techniques for pharmaceutical and bio-analytical purposes. These techniques include 2D-LC and capillary LC hyphenated with mass spectrometry. Another emphasis is on the fundamental investigation of novel chromatographic supports and the development of generic separation strategies for complex samples.</p>',
'image_id' => '247',
'type' => 'Application note',
'url' => 'files/application_notes/AN-rms-07_2017.pdf',
'slug' => 'lc-ms-measurement-of-whole-body-uptake-of-pharmaceuticals-in-zebrafish-application-note',
'meta_keywords' => '',
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'modified' => '2017-07-27 14:19:16',
'created' => '2017-07-27 14:19:16',
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[maximum depth reached]
)
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'id' => '1050',
'name' => 'Spatial tissue proteomics of FFPE specimen using the Bioruptor® for sample preparation',
'description' => '<p><span>Thanks to the excellent preservation of morphological features, formalin fixed and paraffin embedded (FFPE) tissues are an invaluable resource for spatial tissue proteomics. At the same time, due to the chemical crosslinking, it is extremely difficult to lyse such samples and solubilize proteins for deep proteomic analysis. A team of researchers from the EMBL in Heidelberg led by Martin Beck, Stephan Singer (University Hospital Heidelberg and University of Greifswald) and Alessandro Ori (Leibniz Institute on Aging – Fritz Lipmann Institute) has developed a strategy that utilizes the Bioruptor Plus in combination with heat-induced reversing of formalin fixation to enhance the efficient protein extraction from FFPE tissues [1]. This enables the ultrasensitive and highly reproducible proteomic analysis of microdissected FFPE specimen with high spatial resolution. </span></p>',
'image_id' => null,
'type' => 'Application note',
'url' => 'files/application_notes/bioruptor-ffpe-application_note.pdf',
'slug' => 'bioruptor-ffpe-application-note',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2019-05-03 17:39:43',
'created' => '2019-05-03 17:37:10',
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[maximum depth reached]
)
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(int) 6 => array(
'id' => '1057',
'name' => 'Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology',
'description' => '<p>Dr. Kelvin Luk’s lab at the University of Pennsylvania focuses on understanding Parkinson’s disease (PD), a progressive neurodegenerative condition for which there is currently no cure. Their current efforts focus on the biology of midbrain dopamine neurons, PD drug discovery, and the role of protein misfolding in PD. We have demonstrated that the Bioruptor® provides a number of benefits in standardizing and creating representative seeding material that can serve to nucleate inclusions in neurons to better understand protein aggregation and misfolding models in α-synuclein.</p>',
'image_id' => '247',
'type' => 'Application note',
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'slug' => 'standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease',
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[maximum depth reached]
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'name' => 'Datasheet of Bioruptor tubes',
'description' => '<p>Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf',
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'id' => '1155',
'name' => 'Chromatin shearing with the Bioruptor Plus',
'description' => '',
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'type' => 'Manual',
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'slug' => 'chromatin-shearing-guide-bioruptor-plus',
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'modified' => '2022-06-15 16:17:37',
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'id' => '1170',
'name' => 'Critical steps for Bioruptor® maintenance and efficient shearing',
'description' => '',
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'type' => 'Document',
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'slug' => 'critical-steps-bioruptor-maintenance',
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'modified' => '2023-08-31 14:27:41',
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'slug' => '',
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'modified' => '2015-07-24 11:49:49',
'created' => '2014-12-22 03:33:31',
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'id' => '5',
'name' => 'Easy to use',
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'modified' => '2015-07-24 11:50:14',
'created' => '2014-11-11 14:16:51',
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'name' => 'Processing of 3-12 samples',
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'name' => 'Sample size 100 µl – 20 ml',
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'name' => 'Advanced timing control',
'slug' => '',
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'modified' => '2015-07-24 11:58:31',
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'Image' => array(
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'id' => '134',
'name' => 'product/shearing_technologies/bioruptor_plus.jpg',
'alt' => 'Bioruptor Plus Sonication Device',
'modified' => '2020-11-24 16:50:33',
'created' => '2015-06-10 17:28:55',
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'Protocol' => array(
(int) 0 => array(
'id' => '36',
'name' => 'Activity Test - Procedure for immunoprecipitation of native chromatin proteins',
'description' => '<p><span>Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate” team from the University Paris Descartes developed a protocol allowing the immunoprecipitation of chromatin proteins prior the analysis of their activity. They used the Bioruptor</span><span>® </span><span>in the initial step of the procedure to release this specific protein, which is advantageous in preserving protein quality. </span></p>',
'image_id' => '202',
'type' => 'Protocol',
'url' => 'files/protocols/activity_test_bioruptor_protocol.pdf',
'slug' => 'activity-test-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:12',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '28',
'name' => 'Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL',
'description' => '<p><span>For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respectively). To guarantee homogeneity of sonication, the tube holders should always be completely filled with tubes. </span></p>',
'image_id' => '221',
'type' => 'Protocol',
'url' => 'files/protocols/Bacterial_Cell_Disruption_with_Bioruptor_protocol.pdf',
'slug' => 'bacterial-cell-disruption-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2019-04-03 13:31:51',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2',
'name' => 'ChIP - Just 4 steps to foolproofing fragmentation for ChIP using Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p><span>Just follow these four steps for shearing chromatin, and you’ll be well on your way to excellent ChIP results without the troubleshooting headache. </span></p>',
'image_id' => null,
'type' => 'Protocol',
'url' => 'files/protocols/WP-4steps-fragmentation-for-chip-V1_09_14.pdf',
'slug' => 'wp-4steps-fragmentation-for-chip-v1-09-14',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-01-14 14:32:45',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '18',
'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus',
'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>',
'image_id' => '214',
'type' => 'Protocol',
'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf',
'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:41:17',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '27',
'name' => 'Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus',
'description' => '<p>Standard procedure to shear Chromatin from Yeast. </p>',
'image_id' => '218',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_Shearing_in_Yeast_Cells_with_Bioruptor_protocol.pdf',
'slug' => 'chromatin-shearing-in-yeast-cells-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-17 19:32:52',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '10',
'name' => 'FFPE DNA extraction protocol using the Bioruptor®',
'description' => '<p><span>The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, providing an invaluable repository of information for genetic analysis. These samples can be analyzed for a wealth of applications including biomarker discovery, drug development, and cancer research. </span><span>Diagenode’s FFPE DNA Extraction kit is optimized for the extraction of DNA from FFPE tissue sections in conjunction with the Bioruptor</span><span>®</span><span>. </span></p>',
'image_id' => '204',
'type' => 'Protocol',
'url' => 'files/protocols/FFPE_DNA_extraction_protocol.pdf',
'slug' => 'ffpe-dna-extraction-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:42:46',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 8 => array(
'id' => '61',
'name' => 'Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis',
'description' => '<p>Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck Institute for Biochemistry, Martinsried, Germany are using the Bioruptor<span>® </span>for their research using C. elegans, a powerful metazoan model system to study and understand fundamental problems in biology. Traditionally C. elegans research has focused mainly on genetics. However, recent studies suggest that C. elegans can also be a facile system for biochemistry. Tagged proteins can be expressed in vivo and interacting partners can be identified using various biochemical approaches. In addition, protein networks and pathways can be identified by combining genetic screens with biochemistry.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/protocols/mass_spectrometry -Celegans-protocol.pdf',
'slug' => 'mass_spectrometry -Celegans-protocol',
'meta_keywords' => 'mass_spectrometry -Celegans-protocol',
'meta_description' => 'mass_spectrometry -Celegans-protocol',
'modified' => '2016-01-15 12:07:23',
'created' => '2016-01-15 11:55:19',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 9 => array(
'id' => '62',
'name' => 'Mass-Spectrometry: procedure for shotgun proteomics',
'description' => '<p>Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in addition to degradation and removal of unwanted components like nucleic acids. Therefore, it requires mechanical breakdown accompanied by denaturing agents and heating of the samples. Matthias Mann and his team from the Department of Proteomics and Signal Transduction at the Max Plank Institute of Biochemistry developed and employ highly efficient protocols for protein isolation in conjunction with Bioruptor to perform quantitative proteomics.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/protocols/bioruptor-mass_spectometry-protocol.pdf',
'slug' => 'bioruptor-mass_spectometry-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-02-05 14:22:56',
'created' => '2016-02-05 14:22:56',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 10 => array(
'id' => '23',
'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus',
'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>',
'image_id' => '213',
'type' => 'Protocol',
'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf',
'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:40:14',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 11 => array(
'id' => '26',
'name' => 'RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit',
'description' => '<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disruption releases RNA, while homogenization reduces sample viscosity to facilitate RNA purification. Diagenode’s Bioruptor</span><span>® </span><span>Sonicator uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues in one step. Diagenode’s RNA extraction reagent (included in the RNA extraction kit) is used as sonication medium and maintains the integrity of RNA while disrupting cells and dissolving cell components. </span></p>',
'image_id' => '216',
'type' => 'Protocol',
'url' => 'files/protocols/RNA_Extraction_from_Tissue_with_Bioruptor_Standard_Plus_protocol.pdf',
'slug' => 'rna-extraction-from-tissue-with-bioruptor-standard-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:45:55',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 12 => array(
'id' => '43',
'name' => 'TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®',
'description' => '<p><span>The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a native environment. Molecular complexes can then be isolated and used for various applications for the identification of partners. Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes combined the TAP tagging with the Bioruptor</span><span>®</span><span>. This combination is essential for the efficient purification of a chromatin protein. </span></p>',
'image_id' => null,
'type' => 'Protocol',
'url' => 'files/protocols/TAP-TAG-procedure-for-bioruptor.pdf',
'slug' => 'tap-tag-procedure-for-bioruptor',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:40',
'created' => '2015-08-10 10:50:46',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 13 => array(
'id' => '24',
'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®',
'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>',
'image_id' => '220',
'type' => 'Protocol',
'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf',
'slug' => 'western-blot-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:39:29',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4454',
'name' => 'Histone lysine demethylase inhibition reprograms prostate cancermetabolism and mechanics.',
'authors' => 'Chianese Ugo and Papulino Chiara and Passaro Eugenia andEvers Tom Mj and Babaei Mehrad and Toraldo Antonella andDe Marchi Tommaso and Niméus Emma and Carafa Vincenzo andNicoletti Maria Maddalena and Del Gaudio Nunzio andIaccarino Nunzia an',
'description' => '<p>OBJECTIVE: Aberrant activity of androgen receptor (AR) is the primary cause underlying development and progression of prostate cancer (PCa) and castration-resistant PCa (CRPC). Androgen signaling regulates gene transcription and lipid metabolism, facilitating tumor growth and therapy resistance in early and advanced PCa. Although direct AR signaling inhibitors exist, AR expression and function can also be epigenetically regulated. Specifically, lysine (K)-specific demethylases (KDMs), which are often overexpressed in PCa and CRPC phenotypes, regulate the AR transcriptional program. METHODS: We investigated LSD1/UTX inhibition, two KDMs, in PCa and CRPC using a multi-omics approach. We first performed a mitochondrial stress test to evaluate respiratory capacity after treatment with MC3324, a dual KDM-inhibitor, and then carried out lipidomic, proteomic, and metabolic analyses. We also investigated mechanical cellular properties with acoustic force spectroscopy. RESULTS: MC3324 induced a global increase in H3K4me2 and H3K27me3 accompanied by significant growth arrest and apoptosis in androgen-responsive and -unresponsive PCa systems. LSD1/UTX inhibition downregulated AR at both transcriptional and non-transcriptional level, showing cancer selectivity, indicating its potential use in resistance to androgen deprivation therapy. Since MC3324 impaired metabolic activity, by modifying the protein and lipid content in PCa and CRPC cell lines. Epigenetic inhibition of LSD1/UTX disrupted mitochondrial ATP production and mediated lipid plasticity, which affected the phosphocholine class, an important structural element for the cell membrane in PCa and CRPC associated with changes in physical and mechanical properties of cancer cells. CONCLUSIONS: Our data suggest a network in which epigenetics, hormone signaling, metabolite availability, lipid content, and mechano-metabolic process are closely related. This network may be able to identify additional hotspots for pharmacological intervention and underscores the key role of KDM-mediated epigenetic modulation in PCa and CRPC.</p>',
'date' => '2022-08-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35944897',
'doi' => '10.1016/j.molmet.2022.101561',
'modified' => '2022-10-21 09:37:56',
'created' => '2022-09-28 09:53:13',
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[maximum depth reached]
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(int) 1 => array(
'id' => '4214',
'name' => 'Comprehensive characterization of the epigenetic landscape in Multiple Myeloma',
'authors' => 'Elina Alaterre et al.',
'description' => '<p>Background: Human multiple myeloma (MM) cell lines (HMCLs) have been widely used to understand the<br />molecular processes that drive MM biology. Epigenetic modifications are involved in MM development,<br />progression, and drug resistance. A comprehensive characterization of the epigenetic landscape of MM would<br />advance our understanding of MM pathophysiology and may attempt to identify new therapeutic targets.<br />Methods: We performed chromatin immunoprecipitation sequencing to analyze histone mark changes<br />(H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3 and H3K36me3) on 16 HMCLs.<br />Results: Differential analysis of histone modification profiles highlighted links between histone modifications<br />and cytogenetic abnormalities or recurrent mutations. Using histone modifications associated to enhancer<br />regions, we identified super-enhancers (SE) associated with genes involved in MM biology. We also identified<br />promoters of genes enriched in H3K9me3 and H3K27me3 repressive marks associated to potential tumor<br />suppressor functions. The prognostic value of genes associated with repressive domains and SE was used to<br />build two distinct scores identifying high-risk MM patients in two independent cohorts (CoMMpass cohort; n =<br />674 and Montpellier cohort; n = 69). Finally, we explored H3K4me3 marks comparing drug-resistant and<br />-sensitive HMCLs to identify regions involved in drug resistance. From these data, we developed epigenetic<br />biomarkers based on the H3K4me3 modification predicting MM cell response to lenalidomide and histone<br />deacetylase inhibitors (HDACi).<br />Conclusions: The epigenetic landscape of MM cells represents a unique resource for future biological studies.<br />Furthermore, risk-scores based on SE and repressive regions together with epigenetic biomarkers of drug<br />response could represent new tools for precision medicine in MM.</p>',
'date' => '2022-01-16',
'pmid' => 'https://www.thno.org/v12p1715',
'doi' => '10.7150/thno.54453',
'modified' => '2022-01-27 13:17:28',
'created' => '2022-01-27 13:14:17',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4164',
'name' => 'Chromatin dysregulation associated with NSD1 mutation in head and necksquamous cell carcinoma.',
'authors' => 'Farhangdoost, Nargess et al. ',
'description' => '<p>Chromatin dysregulation has emerged as an important mechanism of oncogenesis. To develop targeted treatments, it is important to understand the transcriptomic consequences of mutations in chromatin modifier genes. Recently, mutations in the histone methyltransferase gene nuclear receptor binding SET domain protein 1 (NSD1) have been identified in a subset of common and deadly head and neck squamous cell carcinomas (HNSCCs). Here, we use genome-wide approaches and genome editing to dissect the downstream effects of loss of NSD1 in HNSCC. We demonstrate that NSD1 mutations are responsible for loss of intergenic H3K36me2 domains, followed by loss of DNA methylation and gain of H3K27me3 in the affected genomic regions. In addition, those regions are enriched in cis-regulatory elements, and subsequent loss of H3K27ac correlates with reduced expression of their target genes. Our analysis identifies genes and pathways affected by the loss of NSD1 and paves the way to further understanding the interplay among chromatin modifications in cancer.</p>',
'date' => '2021-02-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33626351',
'doi' => '10.1016/j.celrep.2021.108769',
'modified' => '2021-12-21 15:35:45',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3396',
'name' => 'The Itaconate Pathway Is a Central Regulatory Node Linking Innate Immune Tolerance and Trained Immunity',
'authors' => 'Domínguez-Andrés Jorge, Novakovic Boris, Li Yang, Scicluna Brendon P., Gresnigt Mark S., Arts Rob J.W., Oosting Marije, Moorlag Simone J.C.F.M., Groh Laszlo A., Zwaag Jelle, Koch Rebecca M., ter Horst Rob, Joosten Leo A.B., Wijmenga Cisca, Michelucci Ales',
'description' => '<p>Sepsis involves simultaneous hyperactivation of the immune system and immune paralysis, leading to both organ dysfunction and increased susceptibility to secondary infections. Acute activation of myeloid cells induced itaconate synthesis, which subsequently mediated innate immune tolerance in human monocytes. In contrast, induction of trained immunity by b-glucan counteracted tolerance induced in a model of human endotoxemia by inhibiting the expression of immune-responsive gene 1 (IRG1), the enzyme that controls itaconate synthesis. b-Glucan also increased the expression of succinate dehydrogenase (SDH), contributing to the integrity of the TCA cycle and leading to an enhanced innate immune response after secondary stimulation. The role of itaconate was further validated by IRG1 and SDH polymorphisms that modulate induction of tolerance and trained immunity in human monocytes. These data demonstrate the importance of the IRG1-itaconateSDH axis in the development of immune tolerance and training and highlight the potential of b-glucaninduced trained immunity to revert immunoparalysis.</p>',
'date' => '2018-10-01',
'pmid' => 'http://www.pubmed.gov/30293776',
'doi' => '10.1016/j.cmet.2018.09.003',
'modified' => '2018-11-22 15:18:30',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3377',
'name' => 'Interplay of cell–cell contacts and RhoA/MRTF‐A signaling regulates cardiomyocyte identity',
'authors' => 'Dorn et al',
'description' => '<p><span>Cell–cell and cell–matrix interactions guide organ development and homeostasis by controlling lineage specification and maintenance, but the underlying molecular principles are largely unknown. Here, we show that in human developing cardiomyocytes cell–cell contacts at the intercalated disk connect to remodeling of the actin cytoskeleton by regulating the RhoA‐</span><span class="styled-content fixed-case">ROCK</span><span><span> </span>signaling to maintain an active<span> </span></span><span class="styled-content fixed-case">MRTF</span><span>/</span><span class="styled-content fixed-case">SRF</span><span><span> </span>transcriptional program essential for cardiomyocyte identity. Genetic perturbation of this mechanosensory pathway activates an ectopic fat gene program during cardiomyocyte differentiation, which ultimately primes the cells to switch to the brown/beige adipocyte lineage in response to adipogenesis‐inducing signals. We also demonstrate by<span> </span></span><em>in vivo</em><span><span> </span>fate mapping and clonal analysis of cardiac progenitors that cardiac fat and a subset of cardiac muscle arise from a common precursor expressing Isl1 and Wt1 during heart development, suggesting related mechanisms of determination between the two lineages.</span></p>',
'date' => '2018-05-15',
'pmid' => 'http://emboj.embopress.org/content/early/2018/05/15/embj.201798133',
'doi' => '10.15252/embj.201798133',
'modified' => '2018-05-22 22:50:53',
'created' => '2018-05-22 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3368',
'name' => 'Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity',
'authors' => 'Darya A. Haas, Arno Meiler, Katharina Geiger, Carola Vogt, Ellen Preuss, Georg Kochs, Andreas Pichlmair',
'description' => '<p><span>Viruses have evolved a plethora of mechanisms to target host antiviral responses. Here, we propose a yet uncharacterized mechanism of immune regulation by the orthomyxovirus Thogoto virus (THOV) ML protein through engaging general transcription factor TFIIB. ML generates a TFIIB depleted nuclear environment by re-localizing it into the cytoplasm. Although a broad effect on gene expression would be anticipated, ML expression, delivery of an ML-derived functional domain or experimental depletion of TFIIB only leads to altered expression of a limited number of genes. Our data indicate that TFIIB is critically important for the </span><em>de novo</em><span>recruitment of Pol II to promoter start sites and that TFIIB may not be required for regulated gene expression from paused promoters. Since many immune genes require<span> </span></span><em>de novo</em><span>recruitment of Pol II, targeting of TFIIB by THOV represents a neat mechanism to affect immune responses while keeping other cellular transcriptional activities intact. Thus, interference with TFIIB activity may be a favourable site for therapeutic intervention to control undesirable inflammation.</span></p>',
'date' => '2018-04-30',
'pmid' => 'http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006980',
'doi' => '10.1371/journal.ppat.1006980',
'modified' => '2018-05-09 08:55:23',
'created' => '2018-05-09 08:55:23',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3369',
'name' => 'HMGB2 Loss upon Senescence Entry Disrupts Genomic Organization and Induces CTCF Clustering across Cell Types',
'authors' => 'Zirkel et. al.',
'description' => '<p><span>Processes like cellular senescence are characterized by complex events giving rise to heterogeneous cell populations. However, the early molecular events driving this cascade remain elusive. We hypothesized that senescence entry is triggered by an early disruption of the cells’ three-dimensional (3D) <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/genome-organization" title="Learn more about Genome Organization">genome organization</a>. To test this, we combined Hi-C, single-cell and population <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transcriptome" title="Learn more about Transcriptome">transcriptomics</a>, imaging, and </span><em>in silico</em><span>modeling of three distinct cells types entering senescence. Genes involved in DNA conformation maintenance are suppressed upon senescence entry across all cell types. We show that nuclear depletion of the abundant<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/hmgb2" title="Learn more about HMGB2">HMGB2</a><span> </span>protein occurs early on the path to senescence and coincides with the dramatic spatial clustering of CT</span><em>CF.</em><span>Knocking down<span> </span></span><em>HMGB2</em><span><span> </span>suffices for senescence-induced<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ctcf" title="Learn more about CTCF">CTCF</a><span> </span>clustering and for loop reshuffling, while ectopically expressing<span> </span></span><em>HMGB2</em><span><span> </span>rescues these effects. Our data suggest that HMGB2-mediated genomic reorganization constitutes a<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/primer-molecular-biology" title="Learn more about Primer (molecular biology)">primer</a><span> </span>for the ensuing senescent program.</span></p>',
'date' => '2018-04-26',
'pmid' => 'https://www.sciencedirect.com/science/article/pii/S1097276518302338',
'doi' => '10.1016/j.molcel.2018.03.030',
'modified' => '2018-05-09 22:50:53',
'created' => '2018-05-09 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3337',
'name' => 'Widespread bacterial protein histidine phosphorylation revealed by mass spectrometry-based proteomics',
'authors' => 'Clement M Potel, Miao-Hsia Lin, Albert J R Heck, Simone Lemeer',
'description' => '<p>For decades, major difficulties in analyzing histidine phosphorylation have limited the study of phosphohistidine signaling. Here we report a method revealing widespread and abundant protein histidine phosphorylation in <i>Escherichia coli</i>. We generated an extensive <i>E. coli</i> phosphoproteome data set, in which a remarkably high percentage (<span class="stix"><span class="stix">∼</span></span>10%) of phosphorylation sites are phosphohistidine sites. This resource should help enable a better understanding of the biological function of histidine phosphorylation.</p>',
'date' => '2018-01-31',
'pmid' => 'https://www.nature.com/articles/nmeth.4580',
'doi' => '10.1038/nmeth.4580',
'modified' => '2018-02-11 02:37:05',
'created' => '2018-02-11 02:37:05',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 8 => array(
'id' => '3214',
'name' => 'MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1',
'authors' => 'Ann-Christin Hau, Britta Moyo Grebbin, Zsuzsa Agoston, Marie Anders-Maurer, Tamara Müller, Anja Groß, Jasmine Kolb, Julian D. Langer, Claudia Döring, Dorothea Schulte',
'description' => '<p><span>Pre–B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone–derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes: In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron-specific gene </span><em>doublecortin (Dcx)</em><span>. Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.</span></p>',
'date' => '2017-07-24',
'pmid' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'doi' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'modified' => '2017-08-29 11:17:03',
'created' => '2017-07-29 07:39:31',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 9 => array(
'id' => '3006',
'name' => 'reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells',
'authors' => 'Kinkley S et al.',
'description' => '<p>The combinatorial action of co-localizing chromatin modifications and regulators determines chromatin structure and function. However, identifying co-localizing chromatin features in a high-throughput manner remains a technical challenge. Here we describe a novel reChIP-seq approach and tailored bioinformatic analysis tool, normR that allows for the sequential enrichment and detection of co-localizing DNA-associated proteins in an unbiased and genome-wide manner. We illustrate the utility of the reChIP-seq method and normR by identifying H3K4me3 or H3K27me3 bivalently modified nucleosomes in primary human CD4(+) memory T cells. We unravel widespread bivalency at hypomethylated CpG-islands coinciding with inactive promoters of developmental regulators. reChIP-seq additionally uncovered heterogeneous bivalency in the population, which was undetectable by intersecting H3K4me3 and H3K27me3 ChIP-seq tracks. Finally, we provide evidence that bivalency is established and stabilized by an interplay between the genome and epigenome. Our reChIP-seq approach augments conventional ChIP-seq and is broadly applicable to unravel combinatorial modes of action.</p>',
'date' => '2016-08-17',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27530917',
'doi' => '',
'modified' => '2016-08-26 11:56:46',
'created' => '2016-08-26 11:38:15',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 10 => array(
'id' => '2981',
'name' => 'UMI-4C for quantitative and targeted chromosomal contact profiling',
'authors' => 'Omer Schwartzman, Zohar Mukamel, Noa Oded-Elkayam, Pedro Olivares-Chauvet, Yaniv Lubling, Gilad Landan, Shai Izraeli and Amos Tanay',
'description' => '<p>We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (umis) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.</p>',
'date' => '2016-07-04',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27376768',
'doi' => '10.1038/nmeth.3922',
'modified' => '2016-07-13 09:33:41',
'created' => '2016-07-13 09:32:29',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 11 => array(
'id' => '2911',
'name' => 'Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock',
'authors' => 'Yoon Sik Kim, Young-Beom Kim, Woong Bin Kim, Seung Won Lee, Seog Bae Oh, Hee-Chul Han, C. Justin LeeEmail author, Christopher S. Colwell and Yang In Kim',
'description' => '<h3>Background</h3>
<p>Recent evidence indicates that histamine, acting on histamine 1 receptor (H1R), resets the circadian clock in the mouse suprachiasmatic nucleus (SCN) by increasing intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) through the activation of Ca<sub>V</sub>1.3 L-type Ca<sup>2+</sup> channels and Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release from ryanodine receptor-mediated internal stores.</p>
<h3>Results</h3>
<p>In the current study, we explored the underlying mechanisms with various techniques including Ca<sup>2+</sup>- and Cl<sup>−</sup>-imaging and extracellular single-unit recording. Our hypothesis was that histamine causes Cl<sup>−</sup> efflux through cystic fibrosis transmembrane conductance regulator (CFTR) to elicit membrane depolarization needed for the activation of Ca<sub>V</sub>1.3 Ca<sup>2+</sup> channels in SCN neurons. We found that histamine elicited Cl<sup>−</sup> efflux and increased [Ca<sup>2+</sup>]<sub>i</sub> in dissociated mouse SCN cells. Both of these events were suppressed by bumetanide [Na<sup>+</sup>-K<sup>+</sup>-2Cl<sup>−</sup> cotransporter isotype 1 (NKCC1) blocker], CFTR<sub>inh</sub>-172 (CFTR inhibitor), gallein (G<sub>βγ</sub> protein inhibitor) and H89 [protein kinase A (PKA) inhibitor]. By itself, H1R activation with 2-pyridylethylamine increased the level of cAMP in the SCN and this regulation was prevented by gallein. Finally, histamine-evoked phase shifts of the circadian neural activity rhythm in the mouse SCN slice were blocked by bumetanide, CFTR<sub>inh</sub>-172, gallein or H89 and were not observed in NKCC1 or CFTR KO mice.</p>
<h3>Conclusions</h3>
<p>Taken together, these results indicate that histamine recruits the H1R-G<sub>βγ</sub>-cAMP/PKA pathway in the SCN neurons to activate Ca<sub>V</sub>1.3 channels through CFTR-mediated Cl<sup>−</sup> efflux and ultimately to phase-shift the circadian clock. This pathway and NKCC1 may well be potential targets for agents designed to treat problems resulting from the disturbance of the circadian system.</p>',
'date' => '2016-03-22',
'pmid' => 'http://molecularbrain.biomedcentral.com/articles/10.1186/s13041-016-0227-1',
'doi' => '10.1186/s13041-016-0227-1',
'modified' => '2016-07-05 10:50:21',
'created' => '2016-05-11 07:38:13',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 12 => array(
'id' => '2810',
'name' => 'Standardizing chromatin research: a simple and universal method for ChIP-seq',
'authors' => 'Laura Arrigoni, Andreas S. Richter, Emily Betancourt, Kerstin Bruder, Sarah Diehl, Thomas Manke and Ulrike Bönisch',
'description' => '<p><span>Here we demonstrate that harmonization of ChIP-seq workflows across cell types and conditions is possible when obtaining chromatin from properly isolated nuclei. We established an ultrasound-based nuclei extraction method (Nuclei Extraction by Sonication) that is highly effective across various organisms, cell types and cell numbers. The described method has the potential to replace complex cell-type-specific, but largely ineffective, nuclei isolation protocols. This article demonstrates protocol standardization using the Bioruptor shearing systems and the IP-Star Automation System for ChIP automation.</span></p>',
'date' => '2015-12-23',
'pmid' => 'http://pubmed.gov/26704968',
'doi' => '10.1093/nar/gkv1495',
'modified' => '2016-06-09 09:47:00',
'created' => '2016-01-10 08:32:58',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 13 => array(
'id' => '2804',
'name' => 'HNF1β drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC)',
'authors' => 'Filipa Lopes-Coelho, Sofia Gouveia-Fernandes, Luís G Gonçalves, Carolina Nunes, Inês Faustino, Fernanda Silva, Ana Félix, Sofia A Pereira, Jacinta Serpa',
'description' => '<p>Chemoresistance to platinum-based antineoplastic agents is a consistent feature among ovarian carcinomas; however, whereas high-grade serous carcinoma (OSC) acquires resistance during chemotherapy, ovarian clear cell carcinoma (OCCC) is intrinsically resistant. The main objective of this study was to explore, in vitro and in vivo, if hepatocyte nuclear factor 1β (HNF1β) and glutaminolysis contribute for the resistance of OCCC to carboplatin through the intrinsically increased GSH bioavailability. To disclose the role of HNF1β, experiments were also performed in an OSC cell line, which does not express HNF1β. Metabolic profiles, GSH quantification, HNF1β, and γ-glutamylcysteine ligase catalytic subunit (GCLC) and modifier subunit (GCLM) expression, cell cycle, and death were assessed in ES2 cell line (OCCC) and OVCAR3 cell line (OSC); HNF1β knockdown was performed in ES2 and murine model of subcutaneous and peritoneal OCCC tumors was established to test buthionine sulphoxamine (BSO), as a sensitizer to carboplatin. Glutaminolysis is activated in ES2 and OVCAR3, though ES2 exclusively synthesizes amino acids and GSH. ES2 cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 to carboplatin. HNF1β regulates the expression of GCLC, but not GCLM, and consequently GSH production in ES2. In vivo, BSO prior to carboplatin reduces dramatically subcutaneous tumor size and GSH levels, as well as peritoneal dissemination. Our study discloses HNF1β as the mediator of intrinsic OCCC chemoresistance and sheds a light to re-explore a cancer adjuvant therapeutic approach using BSO to overcome the lack of efficient therapy in OCCC.</p>',
'date' => '2015-10-31',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26520442',
'doi' => '10.1007/s13277-015-4290-5',
'modified' => '2016-01-19 07:13:46',
'created' => '2015-12-13 09:42:34',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 14 => array(
'id' => '2795',
'name' => 'LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2',
'authors' => 'Maryem A. Hussein, Elina Shrestha, Mireille Ouimet, Tessa J. Barrett, Sarah Leone, Kathryn J. Moore, Yann Hérault, Edward A. Fisher, Michael J. Garabedian',
'description' => '<p>High cholesterol and diabetes are major risk factors for atherosclerosis. Regression of atherosclerosis is mediated in part by the Liver X Receptor (LXR) through the induction of genes involved in cholesterol transport and efflux. In the context of diabetes, regression of atherosclerosis is impaired. We proposed that changes in glucose levels modulate LXR-dependent gene expression. Using a mouse macrophage cell line (RAW 264.7) and primary bone marrow derived macrophages (BMDMs) cultured in normal or diabetes relevant high glucose conditions we found that high glucose inhibits the LXR-dependent expression of ATP-binding cassette transporter A1 (ABCA1), but not ABCG1. To probe for this mechanism, we surveyed the expression of a host of chromatin-modifying enzymes and found that Protein Arginine Methyltransferase 2 (PRMT2) was reduced in high compared to normal glucose conditions. Importantly, ABCA1 expression and ABCA1-mediated cholesterol efflux were reduced in Prmt2-/- compared to wild type BMDMs. Monocytes from diabetic mice also showed decreased expression of Prmt2 compared to non-diabetic counterparts. Thus, PRMT2 represents a glucose-sensitive factor that plays a role in LXR-mediated ABCA1-dependent cholesterol efflux and lends insight to the presence of increased atherosclerosis in diabetic patients.</p>',
'date' => '2015-08-19',
'pmid' => 'http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135218',
'doi' => '10.1371/journal.pone.0135218',
'modified' => '2015-11-10 13:43:13',
'created' => '2015-10-27 10:16:07',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 15 => array(
'id' => '2647',
'name' => 'Sensitive detection of chromatin-altering polymorphisms reveals autoimmune disease mechanisms.',
'authors' => 'Del Rosario RC, Poschmann J, Rouam SL, Png E, Khor CC, Hibberd ML, Prabhakar S',
'description' => 'Most disease associations detected by genome-wide association studies (GWAS) lie outside coding genes, but very few have been mapped to causal regulatory variants. Here, we present a method for detecting regulatory quantitative trait loci (QTLs) that does not require genotyping or whole-genome sequencing. The method combines deep, long-read chromatin immunoprecipitation-sequencing (ChIP-seq) with a statistical test that simultaneously scores peak height correlation and allelic imbalance: the genotype-independent signal correlation and imbalance (G-SCI) test. We performed histone acetylation ChIP-seq on 57 human lymphoblastoid cell lines and used the resulting reads to call 500,066 single-nucleotide polymorphisms de novo within regulatory elements. The G-SCI test annotated 8,764 of these as histone acetylation QTLs (haQTLs)-an order of magnitude larger than the set of candidates detected by expression QTL analysis. Lymphoblastoid haQTLs were highly predictive of autoimmune disease mechanisms. Thus, our method facilitates large-scale regulatory variant detection in any moderately sized cohort for which functional profiling data can be generated, thereby simplifying identification of causal variants within GWAS loci.',
'date' => '2015-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25799442',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
'created' => '2015-07-24 15:39:05',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 16 => array(
'id' => '2643',
'name' => 'Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus, Osmeridae), an anadromous Pacific smelt.',
'authors' => 'Candy JR, Campbell NR, Grinnell MH, Beacham TD, Larson WA, Narum SR',
'description' => 'Twelve eulachon (Thaleichthys pacificus, Osmeridae) populations ranging from Cook Inlet, Alaska and along the west coast of North America to the Columbia River were examined by restriction-site-associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high geneflow species. A total of 4104 single-nucleotide polymorphisms (SNPs) were discovered across the genome, with 193 putatively adaptive SNPs as determined by FST outlier tests. Estimates of population structure in eulachon with the putatively adaptive SNPs were similar, but provided greater resolution of stocks compared with a putatively neutral panel of 3911 SNPs or previous estimates with 14 microsatellites. A cline of increasing measures of genetic diversity from south to north was found in the adaptive panel, but not in the neutral markers (SNPs or microsatellites). This may indicate divergent selective pressures in differing freshwater and marine environments between regional eulachon populations and that these adaptive diversity patterns not seen with neutral markers could be a consideration when determining genetic boundaries for conservation purposes. Estimates of effective population size (Ne ) were similar with the neutral SNP panel and microsatellites and may be utilized to monitor population status for eulachon where census sizes are difficult to obtain. Greater differentiation with the panel of putatively adaptive SNPs provided higher individual assignment accuracy compared to the neutral panel or microsatellites for stock identification purposes. This study presents the first SNPs that have been developed for eulachon, and analyses with these markers highlighted the importance of integrating genome-wide neutral and adaptive genetic variation for the applications of conservation and management.',
'date' => '2015-03-03',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25737187',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
'created' => '2015-07-24 15:39:05',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 17 => array(
'id' => '3076',
'name' => 'Silica nanoparticles induce oxidative stress, inflammation, and endothelial dysfunction in vitro via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling.',
'authors' => 'Caixia Guo,1,2 Yinye Xia,1,2 Piye Niu,1,2 Lizhen Jiang,1,2 Junchao Duan,1,2 Yang Yu,1,2 Xianqing Zhou,1,2 Yanbo Li,1,2 Zhiwei Sun1,2 ',
'description' => '<p><strong>Abstract:</strong><span> Despite the widespread application of silica nanoparticles (SiNPs) in industrial, commercial, and biomedical fields, their response to human cells has not been fully elucidated. Overall, little is known about the toxicological effects of SiNPs on the cardiovascular system. In this study, SiNPs with a 58 nm diameter were used to study their interaction with human umbilical vein endothelial cells (HUVECs). Dose- and time-dependent decrease in cell viability and damage on cell plasma-membrane integrity showed the cytotoxic potential of the SiNPs. SiNPs were found to induce oxidative stress, as evidenced by the significant elevation of reactive oxygen species generation and malondialdehyde production and downregulated activity in glutathione peroxidase. SiNPs also stimulated release of cytoprotective nitric oxide (NO) and upregulated inducible nitric oxide synthase (NOS) messenger ribonucleic acid, while downregulating endothelial NOS and ET-1 messenger ribonucleic acid, suggesting that SiNPs disturbed the NO/NOS system. SiNP-induced oxidative stress and NO/NOS imbalance resulted in endothelial dysfunction. SiNPs induced inflammation characterized by the upregulation of key inflammatory mediators, including IL-1β, IL-6, IL-8, TNFα, ICAM-1, VCAM-1, and MCP-1. In addition, SiNPs triggered the activation of the Nrf2-mediated antioxidant system, as evidenced by the induction of nuclear factor-κB and MAPK pathway activation. Our findings demonstrated that SiNPs could induce oxidative stress, inflammation, and NO/NOS system imbalance, and eventually lead to endothelial dysfunction via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling. This study indicated a potential deleterious effect of SiNPs on the vascular endothelium, which warrants more careful assessment of SiNPs before their application. </span></p>',
'date' => '2015-02-20',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25759575',
'doi' => '10.2147/IJN.S76114',
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'description' => 'Nowadays, identification of proteins from biological samples by mass spectrometry is widely used. In principle there are two scenarios. Proteins are pre-fractionated in some way, e.g. by gel electrophoresis or are analyzed as complex mixture (shot gun). Shot gun proteomics became recently more popular, because of technological developments on the mass spectrometer side which allows now the identification of several thousand proteins from complex biological matrix. However, in many cases it is still useful to separate proteins first in a gel. But not only mass spectrometer technology made progress. This is also true for the sample preparation. Recently, protocols and techniques were developed which make the analysis of starting material in the low microgram range possible and also simplify the whole procedure. Detailed protocols will be described allowing also inexperienced beginners to get good results. ',
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'description' => 'Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). Results indicate that μ-protocadherin downregulation occurs during early stages of tumourigenesis and progression into the adenoma-carcinoma sequence. Epigenetic mechanisms are involved in this silencing.',
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'description' => '<p>We use the <strong>Bioruptor</strong> sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The <strong>H3K4me3 antibody</strong> is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.</p>
<p>Diagenode provides not only very good products for research but also an excellent customer support.</p>',
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$testimonials = '<blockquote><p>We use the <strong>Bioruptor</strong> sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The <strong>H3K4me3 antibody</strong> is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.</p>
<p>Diagenode provides not only very good products for research but also an excellent customer support.</p><cite>Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany</cite></blockquote>
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<form action="/en/carts/add/1816" id="CartAdd/1816Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1816" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Single Cycle Valve for Water Cooler</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="single-cycle-valve-for-water-cooler-1-unit" data-reveal-id="cartModal-1816" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Single Cycle Valve for Water Cooler</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/metallic-soundproof-box-1-unit"><img src="/img/product/shearing_technologies/B01200001%20_soundproof_box.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200001</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1790" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1790" id="CartAdd/1790Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1790" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Metallic soundproof box</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="metallic-soundproof-box-1-unit" data-reveal-id="cartModal-1790" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Metallic soundproof box</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-ml-bioruptor-microtubes-500-tubes"><img src="/img/product/shearing_technologies/C30010013_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010013</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2627" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2627" id="CartAdd/2627Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2627" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5 ml Bioruptor<sup>®</sup> Plus Microtubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-ml-bioruptor-microtubes-500-tubes" data-reveal-id="cartModal-2627" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5 ml Bioruptor® Plus Microtubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010009</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Plus TPX tubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/rna-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000010</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2616" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2616" id="CartAdd/2616Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2616" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> RNA Extraction kit for Bioruptor Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
'165',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
'165',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="rna-extraction-kit-50-rxns" data-reveal-id="cartModal-2616" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">RNA Extraction kit for Bioruptor Plus</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/protein-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000020</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2617" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2617" id="CartAdd/2617Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2617" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction kit for Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-kit-50-rxns" data-reveal-id="cartModal-2617" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/protein-extraction-beads-20-g"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000021</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2618" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2618" id="CartAdd/2618Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2618" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction beads</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads',
'C20000021',
'100',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads',
'C20000021',
'100',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-beads-20-g" data-reveal-id="cartModal-2618" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Protein Extraction beads</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/Bioruptor-Plus-DNA-QC-kit"><img src="/img/product/kits/dnaqckit.jpg" alt="dna qc kit" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C40010001</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2835" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2835" id="CartAdd/2835Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2835" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Bioruptor<sup>®</sup> Plus DNA QC kit</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Bioruptor<sup>®</sup> Plus DNA QC kit',
'C40010001',
'135',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Bioruptor<sup>®</sup> Plus DNA QC kit',
'C40010001',
'135',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="Bioruptor-Plus-DNA-QC-kit" data-reveal-id="cartModal-2835" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus DNA QC kit</h6>
</div>
</div>
</li>
'
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'modified' => '2018-01-26 10:57:27',
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'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®',
'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>',
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'description' => 'Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). Results indicate that μ-protocadherin downregulation occurs during early stages of tumourigenesis and progression into the adenoma-carcinoma sequence. Epigenetic mechanisms are involved in this silencing.',
'date' => '0000-00-00',
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p><span></span></p>
<div class="page" title="Page 9">
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'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
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<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">No</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
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<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
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<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
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'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
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<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
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<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
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<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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'old_catalog_number' => 'UCD-300 TM',
'sf_code' => 'B01020001-',
'type' => 'ACC',
'search_order' => '00-Machine',
'price_EUR' => '22500',
'price_USD' => '29500',
'price_GBP' => '19500',
'price_JPY' => '3524625',
'price_CNY' => '/',
'price_AUD' => '73750',
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'Antibody' => array(
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'Related' => array(
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'id' => '1792',
'antibody_id' => null,
'name' => '1.5 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
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'label1' => '',
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'type' => 'ACC',
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'price_JPY' => '203645',
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'price_AUD' => '4625',
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'slug' => '1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack',
'meta_title' => '1.5 ml tube holder for Bioruptor® Standard & Bioruptor® Plus',
'meta_keywords' => '',
'meta_description' => '1.5 ml tube holder for Bioruptor® Standard & Bioruptor® Plus',
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(int) 1 => array(
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'name' => '15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'description' => '<p><span>The attachment for 15 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 15 ml tubes at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200013',
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'type' => 'ACC',
'search_order' => '01-Accessory',
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'price_USD' => '1850',
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'price_JPY' => '250640',
'price_CNY' => '',
'price_AUD' => '4625',
'country' => 'ALL',
'except_countries' => 'Japan',
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'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack',
'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus',
'meta_keywords' => '',
'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus',
'modified' => '2017-08-19 10:36:46',
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'ProductsRelated' => array(
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'name' => '50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'description' => '<p><span>The attachment for 50 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 3 x 50 ml tubes at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 pack',
'catalog_number' => 'B01200014',
'old_catalog_number' => 'UCD-pack 50',
'sf_code' => 'B01200014-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1850',
'price_USD' => '1850',
'price_GBP' => '1600',
'price_JPY' => '289800',
'price_CNY' => '',
'price_AUD' => '4625',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
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'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack',
'meta_title' => '50 ml tube holder for Bioruptor Standard & Bioruptor Plus',
'meta_keywords' => '',
'meta_description' => '50 ml tube holder for Bioruptor Standard & Bioruptor Plus',
'modified' => '2017-08-19 10:37:17',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
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'Image' => array(
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(int) 3 => array(
'id' => '1814',
'antibody_id' => null,
'name' => 'Water cooler, 115V',
'description' => '<p><span>This water cooling system allows continuous (Bioruptor® Standard or Plus) or regulated cooling (Bioruptor® Plus or Pico) of the Bioruptor sonication bath. The regulated valve (</span><a href="http://www.diagenode.com/en/catalog/sonication-54/bioruptor-accessories-56/water-cooler-accessories-60/product/single-cycle-valve-bioruptor-pico-bioruptor-plus--369" target="_blank">Single Cycle valve</a><span>) ensures that water will only be replaced during the OFF cycle to avoid any interference between the water flow and the sonication process.</span></p>',
'label1' => 'Characteristics',
'info1' => '<table>
<tbody>
<tr>
<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
</tr>
<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
</tr>
<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
</tr>
<tr>
<td>Safety classification</td>
<td>Class I/NFL</td>
</tr>
<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
</tr>
<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
</tr>
<tr>
<td>Refrigerant</td>
<td>R290</td>
</tr>
<tr>
<td>Refrigerant quantity</td>
<td>0.04 kg</td>
</tr>
<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
</tr>
<tr>
<td>max. delivery</td>
<td>20 l/min</td>
</tr>
<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
</tr>
<tr>
<td>max. delivery (suction)</td>
<td>17 l/min</td>
</tr>
<tr>
<td>Pump connection</td>
<td>G 1/4" female thread</td>
</tr>
<tr>
<td>min. filling capacity</td>
<td>1.4 l</td>
</tr>
<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
</tr>
<tr>
<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
</tr>
<tr>
<td>Net weight</td>
<td>23 kg</td>
</tr>
<tr>
<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
</tr>
<tr>
<td>max. current</td>
<td>2.8 A</td>
</tr>
<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
</tr>
<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
</tr>
<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
</tr>
</tbody>
</table>',
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'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'B02010003',
'old_catalog_number' => '',
'sf_code' => 'B02010003-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '4850',
'price_USD' => '4950',
'price_GBP' => '4300',
'price_JPY' => '759750',
'price_CNY' => '',
'price_AUD' => '12375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
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'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'water-cooler-115v-1-unit',
'meta_title' => 'Water cooler, 115V',
'meta_keywords' => '',
'meta_description' => 'Water cooler, 115V',
'modified' => '2018-02-14 16:25:25',
'created' => '2015-06-29 14:08:20',
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(int) 4 => array(
'id' => '1813',
'antibody_id' => null,
'name' => 'Water cooler, 230V',
'description' => '<p><span>This water cooling system allows continuous (Bioruptor® Standard or Plus) or regulated cooling (Bioruptor® Plus or Pico) of the Bioruptor sonication bath. The regulated valve (</span><a href="http://www.diagenode.com/en/catalog/sonication-54/bioruptor-accessories-56/water-cooler-accessories-60/product/single-cycle-valve-bioruptor-pico-bioruptor-plus--369" target="_blank">Single Cycle valve</a><span>) ensures that water will only be replaced during the OFF cycle to avoid any interference between the water flow and the sonication process.</span></p>',
'label1' => 'Characteristics',
'info1' => '<table>
<tbody>
<tr>
<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
</tr>
<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
</tr>
<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
</tr>
<tr>
<td>Safety classification</td>
<td>Class I/NFL</td>
</tr>
<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
</tr>
<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
</tr>
<tr>
<td>Refrigerant</td>
<td>R290</td>
</tr>
<tr>
<td>Refrigerant quantity</td>
<td>0.04 kg</td>
</tr>
<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
</tr>
<tr>
<td>max. delivery</td>
<td>20 l/min</td>
</tr>
<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
</tr>
<tr>
<td>max. delivery (suction)</td>
<td>17 l/min</td>
</tr>
<tr>
<td>Pump connection</td>
<td>G 1/4" female thread</td>
</tr>
<tr>
<td>min. filling capacity</td>
<td>1.4 l</td>
</tr>
<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
</tr>
<tr>
<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
</tr>
<tr>
<td>Net weight</td>
<td>23 kg</td>
</tr>
<tr>
<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
</tr>
<tr>
<td>max. current</td>
<td>2.8 A</td>
</tr>
<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
</tr>
<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
</tr>
<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
</tr>
</tbody>
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'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
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'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
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<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
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'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
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'name' => 'RNA extraction',
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. <strong>Diagenode's Bioruptor</strong><sup>® </sup><strong>Plus</strong> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.</div>
<div class="large-12 columns"></div>
<div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/pro-rna-extract-fig3a.png" /></div>
<div class="small-6 medium-6 large-6 columns text-center end small-offset-3"><small><strong>Efficient extraction of pure RNA with high RIN. </strong><br /><span>Total RNA profiles from mouse brain. Tissue was disrupted with Bioruptor<sup>®</sup> Plus as described in the protocol and analyzed on BioAnalyzer (Agilent). Note that small RNAs are present in all profiles indicating that the RNA is largely intact.</span></small></div>
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'meta_description' => 'Diagenode's Bioruptor® Plus uses state-of-the-art ultrasound ACT (Adaptive Cavitation Technology) combined with RNA extraction reagent to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.',
'meta_title' => 'RNA extraction for Next Generation Sequencing using Diagenode's Bioruptor® sonication device | Diagenode',
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div>
</div>
<p></p>
<div class="row">
<div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" />
<p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p>
</div>
<div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" />
<p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p>
</div>
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'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.',
'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode',
'modified' => '2017-10-16 14:39:42',
'created' => '2014-07-02 04:41:03',
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'name' => 'FFPE DNA extraction',
'description' => '<div class="row">
<div class="large-12 columns">Diagenode's high yields FFPE DNA extraction using Bioruptor<sup><span>®</span></sup> is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by traditional methods or can be sheared with the Bioruptor<sup>®</sup> Pico ultrasonicator for downstream NGS library prep using the MicroPlex Library Preparation Kit.</div>
<div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/ffpe_workflow.png" /></div>
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'meta_description' => 'Diagenode's high yields FFPE DNA extraction using Bioruptor is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no tox',
'meta_title' => 'FFPE DNA extraction using Bioruptor® ultrasonicator | Diagenode',
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<div class="small-12 medium-12 large-12 columns"><br />
<p><span>Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</span></p>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/TAB-BR-comparaison.pdf" target="_blank"><img src="https://www.diagenode.com/img/bouton-comparaison.png" /></a></p>
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<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">Technology explained</h2>
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<p><span> <br /></span></p>
<div class="spacer"></div>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;">Reproductibility is our priority</h2>
</div>
</div>
<div><img src="https://www.diagenode.com/img/shearing/reproductibility.png" alt="reproductibility" />
<p class="bottom_note"></p>
</div>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;">An affordable instrument for wide range of applications</h2>
</div>
</div>
<p style="text-align: center;">Designed for any researchers, the Bioruptor gives the user the right level of flexibility.</p>
<table style="width: 972px;">
<tbody>
<tr style="height: 56px;">
<th style="width: 380px; height: 56px;"></th>
<th class="text-center" style="width: 126px; height: 56px;">Bioruptor</th>
<th class="text-center" style="width: 141px; height: 56px;">Cup Horn Sonicators</th>
<th class="text-center" style="width: 156px; height: 56px;">Focused <br />ultra-sonicators</th>
<th class="text-center" style="width: 155px; height: 56px;">Multi Sample Sonicator</th>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Instrument pricing</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Consumables pricing</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Range of applications</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Scalable and sample volume flexibility</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Throughput</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
</tbody>
</table>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">Bioruptor ultrasonication for best results in:</h2>
<p><b><span>✓ Chromatin shearing</span><span> </span><span style="font-weight: 400;">- Industry leader in accurate and tight fragment ranges</span></b></p>
<p><b><span>✓ DNA shearing</span><span> </span><span style="font-weight: 400;">- Excellent results for optimal fragment lengths in NGS library prep</span></b></p>
<p><b><span>✓<span> </span></span>Protein aggregation studies </b><span style="font-weight: 400;">- Standardizing seeding with the robust Bioruptor.<br /></span><i><span style="font-weight: 400;">Read the app note by Dr. Kelvin Luk at the University of Pennsylvania </span></i><a href="https://www.diagenode.com/en/documents/standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease" style="color: #13b29c;"><i><span style="font-weight: 400;">“Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>3D genome analysis with Hi-C</b><span style="font-weight: 400;"> - Preparing chromatin libraries with high-quality sonication.<br /></span><i><span style="font-weight: 400;">Read the app note, “</span></i><a href="https://www.diagenode.com/en/documents/applicationnote-arima-low-input" style="color: #13b29c;"><i><span style="font-weight: 400;">Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>Mass spectrometry</b> <b>and increasing protein identification</b><span style="font-weight: 400;">- Sample preparation using Preomics iST and Bioruptor sonication.<br /></span><i><span style="font-weight: 400;">Read the app note “</span></i><a href="https://www.diagenode.com/en/documents/wp-ist-adaptators" style="color: #13b29c;"><i><span style="font-weight: 400;">Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder”</span></i></a></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>Cell lysis, liposome prep, protein extraction, RNA extraction and more</b></span></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>CUT&RUN –Sonication of input DNA (for enrichment comparison) for NGS</b></span></p>
</div>
</div>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
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'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
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<div class="section">
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<p><span>Disruption of bacterial cells is required for releasing biological molecules from within the cells for various biological applications. Ultrasound technology has been widely used for such cell lysis applications. Ultrasonic disruption is based on cavitation, the creation of cavities in cell suspension. These cavities or small bubbles of dissolved gases or vapors arise from the alteration of pressure in liquids. The bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming leading to mechanical stress that effectively lyses cells. Diagenode’s Bioruptor</span><span><sup>®</sup> </span><span>sonicaton device uses state-of-the-art ultrasound technology to disrupt cells for biological, chemical, pharmaceutical, and industrial applications. </span></p>
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'name' => 'Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL',
'description' => '<p><span>For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respectively). To guarantee homogeneity of sonication, the tube holders should always be completely filled with tubes. </span></p>',
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'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
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'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
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'description' => '<p><span>The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, providing an invaluable repository of information for genetic analysis. These samples can be analyzed for a wealth of applications including biomarker discovery, drug development, and cancer research. </span><span>Diagenode’s FFPE DNA Extraction kit is optimized for the extraction of DNA from FFPE tissue sections in conjunction with the Bioruptor</span><span>®</span><span>. </span></p>',
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'description' => '<p>Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck Institute for Biochemistry, Martinsried, Germany are using the Bioruptor<span>® </span>for their research using C. elegans, a powerful metazoan model system to study and understand fundamental problems in biology. Traditionally C. elegans research has focused mainly on genetics. However, recent studies suggest that C. elegans can also be a facile system for biochemistry. Tagged proteins can be expressed in vivo and interacting partners can be identified using various biochemical approaches. In addition, protein networks and pathways can be identified by combining genetic screens with biochemistry.</p>',
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'name' => 'Mass-Spectrometry: procedure for shotgun proteomics',
'description' => '<p>Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in addition to degradation and removal of unwanted components like nucleic acids. Therefore, it requires mechanical breakdown accompanied by denaturing agents and heating of the samples. Matthias Mann and his team from the Department of Proteomics and Signal Transduction at the Max Plank Institute of Biochemistry developed and employ highly efficient protocols for protein isolation in conjunction with Bioruptor to perform quantitative proteomics.</p>',
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'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus',
'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>',
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'name' => 'RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit',
'description' => '<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disruption releases RNA, while homogenization reduces sample viscosity to facilitate RNA purification. Diagenode’s Bioruptor</span><span>® </span><span>Sonicator uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues in one step. Diagenode’s RNA extraction reagent (included in the RNA extraction kit) is used as sonication medium and maintains the integrity of RNA while disrupting cells and dissolving cell components. </span></p>',
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'name' => 'TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®',
'description' => '<p><span>The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a native environment. Molecular complexes can then be isolated and used for various applications for the identification of partners. Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes combined the TAP tagging with the Bioruptor</span><span>®</span><span>. This combination is essential for the efficient purification of a chromatin protein. </span></p>',
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'slug' => 'tap-tag-procedure-for-bioruptor',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:40',
'created' => '2015-08-10 10:50:46',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 13 => array(
'id' => '24',
'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®',
'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>',
'image_id' => '220',
'type' => 'Protocol',
'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf',
'slug' => 'western-blot-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:39:29',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4454',
'name' => 'Histone lysine demethylase inhibition reprograms prostate cancermetabolism and mechanics.',
'authors' => 'Chianese Ugo and Papulino Chiara and Passaro Eugenia andEvers Tom Mj and Babaei Mehrad and Toraldo Antonella andDe Marchi Tommaso and Niméus Emma and Carafa Vincenzo andNicoletti Maria Maddalena and Del Gaudio Nunzio andIaccarino Nunzia an',
'description' => '<p>OBJECTIVE: Aberrant activity of androgen receptor (AR) is the primary cause underlying development and progression of prostate cancer (PCa) and castration-resistant PCa (CRPC). Androgen signaling regulates gene transcription and lipid metabolism, facilitating tumor growth and therapy resistance in early and advanced PCa. Although direct AR signaling inhibitors exist, AR expression and function can also be epigenetically regulated. Specifically, lysine (K)-specific demethylases (KDMs), which are often overexpressed in PCa and CRPC phenotypes, regulate the AR transcriptional program. METHODS: We investigated LSD1/UTX inhibition, two KDMs, in PCa and CRPC using a multi-omics approach. We first performed a mitochondrial stress test to evaluate respiratory capacity after treatment with MC3324, a dual KDM-inhibitor, and then carried out lipidomic, proteomic, and metabolic analyses. We also investigated mechanical cellular properties with acoustic force spectroscopy. RESULTS: MC3324 induced a global increase in H3K4me2 and H3K27me3 accompanied by significant growth arrest and apoptosis in androgen-responsive and -unresponsive PCa systems. LSD1/UTX inhibition downregulated AR at both transcriptional and non-transcriptional level, showing cancer selectivity, indicating its potential use in resistance to androgen deprivation therapy. Since MC3324 impaired metabolic activity, by modifying the protein and lipid content in PCa and CRPC cell lines. Epigenetic inhibition of LSD1/UTX disrupted mitochondrial ATP production and mediated lipid plasticity, which affected the phosphocholine class, an important structural element for the cell membrane in PCa and CRPC associated with changes in physical and mechanical properties of cancer cells. CONCLUSIONS: Our data suggest a network in which epigenetics, hormone signaling, metabolite availability, lipid content, and mechano-metabolic process are closely related. This network may be able to identify additional hotspots for pharmacological intervention and underscores the key role of KDM-mediated epigenetic modulation in PCa and CRPC.</p>',
'date' => '2022-08-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35944897',
'doi' => '10.1016/j.molmet.2022.101561',
'modified' => '2022-10-21 09:37:56',
'created' => '2022-09-28 09:53:13',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4214',
'name' => 'Comprehensive characterization of the epigenetic landscape in Multiple Myeloma',
'authors' => 'Elina Alaterre et al.',
'description' => '<p>Background: Human multiple myeloma (MM) cell lines (HMCLs) have been widely used to understand the<br />molecular processes that drive MM biology. Epigenetic modifications are involved in MM development,<br />progression, and drug resistance. A comprehensive characterization of the epigenetic landscape of MM would<br />advance our understanding of MM pathophysiology and may attempt to identify new therapeutic targets.<br />Methods: We performed chromatin immunoprecipitation sequencing to analyze histone mark changes<br />(H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3 and H3K36me3) on 16 HMCLs.<br />Results: Differential analysis of histone modification profiles highlighted links between histone modifications<br />and cytogenetic abnormalities or recurrent mutations. Using histone modifications associated to enhancer<br />regions, we identified super-enhancers (SE) associated with genes involved in MM biology. We also identified<br />promoters of genes enriched in H3K9me3 and H3K27me3 repressive marks associated to potential tumor<br />suppressor functions. The prognostic value of genes associated with repressive domains and SE was used to<br />build two distinct scores identifying high-risk MM patients in two independent cohorts (CoMMpass cohort; n =<br />674 and Montpellier cohort; n = 69). Finally, we explored H3K4me3 marks comparing drug-resistant and<br />-sensitive HMCLs to identify regions involved in drug resistance. From these data, we developed epigenetic<br />biomarkers based on the H3K4me3 modification predicting MM cell response to lenalidomide and histone<br />deacetylase inhibitors (HDACi).<br />Conclusions: The epigenetic landscape of MM cells represents a unique resource for future biological studies.<br />Furthermore, risk-scores based on SE and repressive regions together with epigenetic biomarkers of drug<br />response could represent new tools for precision medicine in MM.</p>',
'date' => '2022-01-16',
'pmid' => 'https://www.thno.org/v12p1715',
'doi' => '10.7150/thno.54453',
'modified' => '2022-01-27 13:17:28',
'created' => '2022-01-27 13:14:17',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4164',
'name' => 'Chromatin dysregulation associated with NSD1 mutation in head and necksquamous cell carcinoma.',
'authors' => 'Farhangdoost, Nargess et al. ',
'description' => '<p>Chromatin dysregulation has emerged as an important mechanism of oncogenesis. To develop targeted treatments, it is important to understand the transcriptomic consequences of mutations in chromatin modifier genes. Recently, mutations in the histone methyltransferase gene nuclear receptor binding SET domain protein 1 (NSD1) have been identified in a subset of common and deadly head and neck squamous cell carcinomas (HNSCCs). Here, we use genome-wide approaches and genome editing to dissect the downstream effects of loss of NSD1 in HNSCC. We demonstrate that NSD1 mutations are responsible for loss of intergenic H3K36me2 domains, followed by loss of DNA methylation and gain of H3K27me3 in the affected genomic regions. In addition, those regions are enriched in cis-regulatory elements, and subsequent loss of H3K27ac correlates with reduced expression of their target genes. Our analysis identifies genes and pathways affected by the loss of NSD1 and paves the way to further understanding the interplay among chromatin modifications in cancer.</p>',
'date' => '2021-02-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33626351',
'doi' => '10.1016/j.celrep.2021.108769',
'modified' => '2021-12-21 15:35:45',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3396',
'name' => 'The Itaconate Pathway Is a Central Regulatory Node Linking Innate Immune Tolerance and Trained Immunity',
'authors' => 'Domínguez-Andrés Jorge, Novakovic Boris, Li Yang, Scicluna Brendon P., Gresnigt Mark S., Arts Rob J.W., Oosting Marije, Moorlag Simone J.C.F.M., Groh Laszlo A., Zwaag Jelle, Koch Rebecca M., ter Horst Rob, Joosten Leo A.B., Wijmenga Cisca, Michelucci Ales',
'description' => '<p>Sepsis involves simultaneous hyperactivation of the immune system and immune paralysis, leading to both organ dysfunction and increased susceptibility to secondary infections. Acute activation of myeloid cells induced itaconate synthesis, which subsequently mediated innate immune tolerance in human monocytes. In contrast, induction of trained immunity by b-glucan counteracted tolerance induced in a model of human endotoxemia by inhibiting the expression of immune-responsive gene 1 (IRG1), the enzyme that controls itaconate synthesis. b-Glucan also increased the expression of succinate dehydrogenase (SDH), contributing to the integrity of the TCA cycle and leading to an enhanced innate immune response after secondary stimulation. The role of itaconate was further validated by IRG1 and SDH polymorphisms that modulate induction of tolerance and trained immunity in human monocytes. These data demonstrate the importance of the IRG1-itaconateSDH axis in the development of immune tolerance and training and highlight the potential of b-glucaninduced trained immunity to revert immunoparalysis.</p>',
'date' => '2018-10-01',
'pmid' => 'http://www.pubmed.gov/30293776',
'doi' => '10.1016/j.cmet.2018.09.003',
'modified' => '2018-11-22 15:18:30',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3377',
'name' => 'Interplay of cell–cell contacts and RhoA/MRTF‐A signaling regulates cardiomyocyte identity',
'authors' => 'Dorn et al',
'description' => '<p><span>Cell–cell and cell–matrix interactions guide organ development and homeostasis by controlling lineage specification and maintenance, but the underlying molecular principles are largely unknown. Here, we show that in human developing cardiomyocytes cell–cell contacts at the intercalated disk connect to remodeling of the actin cytoskeleton by regulating the RhoA‐</span><span class="styled-content fixed-case">ROCK</span><span><span> </span>signaling to maintain an active<span> </span></span><span class="styled-content fixed-case">MRTF</span><span>/</span><span class="styled-content fixed-case">SRF</span><span><span> </span>transcriptional program essential for cardiomyocyte identity. Genetic perturbation of this mechanosensory pathway activates an ectopic fat gene program during cardiomyocyte differentiation, which ultimately primes the cells to switch to the brown/beige adipocyte lineage in response to adipogenesis‐inducing signals. We also demonstrate by<span> </span></span><em>in vivo</em><span><span> </span>fate mapping and clonal analysis of cardiac progenitors that cardiac fat and a subset of cardiac muscle arise from a common precursor expressing Isl1 and Wt1 during heart development, suggesting related mechanisms of determination between the two lineages.</span></p>',
'date' => '2018-05-15',
'pmid' => 'http://emboj.embopress.org/content/early/2018/05/15/embj.201798133',
'doi' => '10.15252/embj.201798133',
'modified' => '2018-05-22 22:50:53',
'created' => '2018-05-22 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3368',
'name' => 'Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity',
'authors' => 'Darya A. Haas, Arno Meiler, Katharina Geiger, Carola Vogt, Ellen Preuss, Georg Kochs, Andreas Pichlmair',
'description' => '<p><span>Viruses have evolved a plethora of mechanisms to target host antiviral responses. Here, we propose a yet uncharacterized mechanism of immune regulation by the orthomyxovirus Thogoto virus (THOV) ML protein through engaging general transcription factor TFIIB. ML generates a TFIIB depleted nuclear environment by re-localizing it into the cytoplasm. Although a broad effect on gene expression would be anticipated, ML expression, delivery of an ML-derived functional domain or experimental depletion of TFIIB only leads to altered expression of a limited number of genes. Our data indicate that TFIIB is critically important for the </span><em>de novo</em><span>recruitment of Pol II to promoter start sites and that TFIIB may not be required for regulated gene expression from paused promoters. Since many immune genes require<span> </span></span><em>de novo</em><span>recruitment of Pol II, targeting of TFIIB by THOV represents a neat mechanism to affect immune responses while keeping other cellular transcriptional activities intact. Thus, interference with TFIIB activity may be a favourable site for therapeutic intervention to control undesirable inflammation.</span></p>',
'date' => '2018-04-30',
'pmid' => 'http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006980',
'doi' => '10.1371/journal.ppat.1006980',
'modified' => '2018-05-09 08:55:23',
'created' => '2018-05-09 08:55:23',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3369',
'name' => 'HMGB2 Loss upon Senescence Entry Disrupts Genomic Organization and Induces CTCF Clustering across Cell Types',
'authors' => 'Zirkel et. al.',
'description' => '<p><span>Processes like cellular senescence are characterized by complex events giving rise to heterogeneous cell populations. However, the early molecular events driving this cascade remain elusive. We hypothesized that senescence entry is triggered by an early disruption of the cells’ three-dimensional (3D) <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/genome-organization" title="Learn more about Genome Organization">genome organization</a>. To test this, we combined Hi-C, single-cell and population <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transcriptome" title="Learn more about Transcriptome">transcriptomics</a>, imaging, and </span><em>in silico</em><span>modeling of three distinct cells types entering senescence. Genes involved in DNA conformation maintenance are suppressed upon senescence entry across all cell types. We show that nuclear depletion of the abundant<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/hmgb2" title="Learn more about HMGB2">HMGB2</a><span> </span>protein occurs early on the path to senescence and coincides with the dramatic spatial clustering of CT</span><em>CF.</em><span>Knocking down<span> </span></span><em>HMGB2</em><span><span> </span>suffices for senescence-induced<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ctcf" title="Learn more about CTCF">CTCF</a><span> </span>clustering and for loop reshuffling, while ectopically expressing<span> </span></span><em>HMGB2</em><span><span> </span>rescues these effects. Our data suggest that HMGB2-mediated genomic reorganization constitutes a<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/primer-molecular-biology" title="Learn more about Primer (molecular biology)">primer</a><span> </span>for the ensuing senescent program.</span></p>',
'date' => '2018-04-26',
'pmid' => 'https://www.sciencedirect.com/science/article/pii/S1097276518302338',
'doi' => '10.1016/j.molcel.2018.03.030',
'modified' => '2018-05-09 22:50:53',
'created' => '2018-05-09 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3337',
'name' => 'Widespread bacterial protein histidine phosphorylation revealed by mass spectrometry-based proteomics',
'authors' => 'Clement M Potel, Miao-Hsia Lin, Albert J R Heck, Simone Lemeer',
'description' => '<p>For decades, major difficulties in analyzing histidine phosphorylation have limited the study of phosphohistidine signaling. Here we report a method revealing widespread and abundant protein histidine phosphorylation in <i>Escherichia coli</i>. We generated an extensive <i>E. coli</i> phosphoproteome data set, in which a remarkably high percentage (<span class="stix"><span class="stix">∼</span></span>10%) of phosphorylation sites are phosphohistidine sites. This resource should help enable a better understanding of the biological function of histidine phosphorylation.</p>',
'date' => '2018-01-31',
'pmid' => 'https://www.nature.com/articles/nmeth.4580',
'doi' => '10.1038/nmeth.4580',
'modified' => '2018-02-11 02:37:05',
'created' => '2018-02-11 02:37:05',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 8 => array(
'id' => '3214',
'name' => 'MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1',
'authors' => 'Ann-Christin Hau, Britta Moyo Grebbin, Zsuzsa Agoston, Marie Anders-Maurer, Tamara Müller, Anja Groß, Jasmine Kolb, Julian D. Langer, Claudia Döring, Dorothea Schulte',
'description' => '<p><span>Pre–B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone–derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes: In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron-specific gene </span><em>doublecortin (Dcx)</em><span>. Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.</span></p>',
'date' => '2017-07-24',
'pmid' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'doi' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'modified' => '2017-08-29 11:17:03',
'created' => '2017-07-29 07:39:31',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 9 => array(
'id' => '3006',
'name' => 'reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells',
'authors' => 'Kinkley S et al.',
'description' => '<p>The combinatorial action of co-localizing chromatin modifications and regulators determines chromatin structure and function. However, identifying co-localizing chromatin features in a high-throughput manner remains a technical challenge. Here we describe a novel reChIP-seq approach and tailored bioinformatic analysis tool, normR that allows for the sequential enrichment and detection of co-localizing DNA-associated proteins in an unbiased and genome-wide manner. We illustrate the utility of the reChIP-seq method and normR by identifying H3K4me3 or H3K27me3 bivalently modified nucleosomes in primary human CD4(+) memory T cells. We unravel widespread bivalency at hypomethylated CpG-islands coinciding with inactive promoters of developmental regulators. reChIP-seq additionally uncovered heterogeneous bivalency in the population, which was undetectable by intersecting H3K4me3 and H3K27me3 ChIP-seq tracks. Finally, we provide evidence that bivalency is established and stabilized by an interplay between the genome and epigenome. Our reChIP-seq approach augments conventional ChIP-seq and is broadly applicable to unravel combinatorial modes of action.</p>',
'date' => '2016-08-17',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27530917',
'doi' => '',
'modified' => '2016-08-26 11:56:46',
'created' => '2016-08-26 11:38:15',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 10 => array(
'id' => '2981',
'name' => 'UMI-4C for quantitative and targeted chromosomal contact profiling',
'authors' => 'Omer Schwartzman, Zohar Mukamel, Noa Oded-Elkayam, Pedro Olivares-Chauvet, Yaniv Lubling, Gilad Landan, Shai Izraeli and Amos Tanay',
'description' => '<p>We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (umis) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.</p>',
'date' => '2016-07-04',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27376768',
'doi' => '10.1038/nmeth.3922',
'modified' => '2016-07-13 09:33:41',
'created' => '2016-07-13 09:32:29',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 11 => array(
'id' => '2911',
'name' => 'Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock',
'authors' => 'Yoon Sik Kim, Young-Beom Kim, Woong Bin Kim, Seung Won Lee, Seog Bae Oh, Hee-Chul Han, C. Justin LeeEmail author, Christopher S. Colwell and Yang In Kim',
'description' => '<h3>Background</h3>
<p>Recent evidence indicates that histamine, acting on histamine 1 receptor (H1R), resets the circadian clock in the mouse suprachiasmatic nucleus (SCN) by increasing intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) through the activation of Ca<sub>V</sub>1.3 L-type Ca<sup>2+</sup> channels and Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release from ryanodine receptor-mediated internal stores.</p>
<h3>Results</h3>
<p>In the current study, we explored the underlying mechanisms with various techniques including Ca<sup>2+</sup>- and Cl<sup>−</sup>-imaging and extracellular single-unit recording. Our hypothesis was that histamine causes Cl<sup>−</sup> efflux through cystic fibrosis transmembrane conductance regulator (CFTR) to elicit membrane depolarization needed for the activation of Ca<sub>V</sub>1.3 Ca<sup>2+</sup> channels in SCN neurons. We found that histamine elicited Cl<sup>−</sup> efflux and increased [Ca<sup>2+</sup>]<sub>i</sub> in dissociated mouse SCN cells. Both of these events were suppressed by bumetanide [Na<sup>+</sup>-K<sup>+</sup>-2Cl<sup>−</sup> cotransporter isotype 1 (NKCC1) blocker], CFTR<sub>inh</sub>-172 (CFTR inhibitor), gallein (G<sub>βγ</sub> protein inhibitor) and H89 [protein kinase A (PKA) inhibitor]. By itself, H1R activation with 2-pyridylethylamine increased the level of cAMP in the SCN and this regulation was prevented by gallein. Finally, histamine-evoked phase shifts of the circadian neural activity rhythm in the mouse SCN slice were blocked by bumetanide, CFTR<sub>inh</sub>-172, gallein or H89 and were not observed in NKCC1 or CFTR KO mice.</p>
<h3>Conclusions</h3>
<p>Taken together, these results indicate that histamine recruits the H1R-G<sub>βγ</sub>-cAMP/PKA pathway in the SCN neurons to activate Ca<sub>V</sub>1.3 channels through CFTR-mediated Cl<sup>−</sup> efflux and ultimately to phase-shift the circadian clock. This pathway and NKCC1 may well be potential targets for agents designed to treat problems resulting from the disturbance of the circadian system.</p>',
'date' => '2016-03-22',
'pmid' => 'http://molecularbrain.biomedcentral.com/articles/10.1186/s13041-016-0227-1',
'doi' => '10.1186/s13041-016-0227-1',
'modified' => '2016-07-05 10:50:21',
'created' => '2016-05-11 07:38:13',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 12 => array(
'id' => '2810',
'name' => 'Standardizing chromatin research: a simple and universal method for ChIP-seq',
'authors' => 'Laura Arrigoni, Andreas S. Richter, Emily Betancourt, Kerstin Bruder, Sarah Diehl, Thomas Manke and Ulrike Bönisch',
'description' => '<p><span>Here we demonstrate that harmonization of ChIP-seq workflows across cell types and conditions is possible when obtaining chromatin from properly isolated nuclei. We established an ultrasound-based nuclei extraction method (Nuclei Extraction by Sonication) that is highly effective across various organisms, cell types and cell numbers. The described method has the potential to replace complex cell-type-specific, but largely ineffective, nuclei isolation protocols. This article demonstrates protocol standardization using the Bioruptor shearing systems and the IP-Star Automation System for ChIP automation.</span></p>',
'date' => '2015-12-23',
'pmid' => 'http://pubmed.gov/26704968',
'doi' => '10.1093/nar/gkv1495',
'modified' => '2016-06-09 09:47:00',
'created' => '2016-01-10 08:32:58',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 13 => array(
'id' => '2804',
'name' => 'HNF1β drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC)',
'authors' => 'Filipa Lopes-Coelho, Sofia Gouveia-Fernandes, Luís G Gonçalves, Carolina Nunes, Inês Faustino, Fernanda Silva, Ana Félix, Sofia A Pereira, Jacinta Serpa',
'description' => '<p>Chemoresistance to platinum-based antineoplastic agents is a consistent feature among ovarian carcinomas; however, whereas high-grade serous carcinoma (OSC) acquires resistance during chemotherapy, ovarian clear cell carcinoma (OCCC) is intrinsically resistant. The main objective of this study was to explore, in vitro and in vivo, if hepatocyte nuclear factor 1β (HNF1β) and glutaminolysis contribute for the resistance of OCCC to carboplatin through the intrinsically increased GSH bioavailability. To disclose the role of HNF1β, experiments were also performed in an OSC cell line, which does not express HNF1β. Metabolic profiles, GSH quantification, HNF1β, and γ-glutamylcysteine ligase catalytic subunit (GCLC) and modifier subunit (GCLM) expression, cell cycle, and death were assessed in ES2 cell line (OCCC) and OVCAR3 cell line (OSC); HNF1β knockdown was performed in ES2 and murine model of subcutaneous and peritoneal OCCC tumors was established to test buthionine sulphoxamine (BSO), as a sensitizer to carboplatin. Glutaminolysis is activated in ES2 and OVCAR3, though ES2 exclusively synthesizes amino acids and GSH. ES2 cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 to carboplatin. HNF1β regulates the expression of GCLC, but not GCLM, and consequently GSH production in ES2. In vivo, BSO prior to carboplatin reduces dramatically subcutaneous tumor size and GSH levels, as well as peritoneal dissemination. Our study discloses HNF1β as the mediator of intrinsic OCCC chemoresistance and sheds a light to re-explore a cancer adjuvant therapeutic approach using BSO to overcome the lack of efficient therapy in OCCC.</p>',
'date' => '2015-10-31',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26520442',
'doi' => '10.1007/s13277-015-4290-5',
'modified' => '2016-01-19 07:13:46',
'created' => '2015-12-13 09:42:34',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 14 => array(
'id' => '2795',
'name' => 'LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2',
'authors' => 'Maryem A. Hussein, Elina Shrestha, Mireille Ouimet, Tessa J. Barrett, Sarah Leone, Kathryn J. Moore, Yann Hérault, Edward A. Fisher, Michael J. Garabedian',
'description' => '<p>High cholesterol and diabetes are major risk factors for atherosclerosis. Regression of atherosclerosis is mediated in part by the Liver X Receptor (LXR) through the induction of genes involved in cholesterol transport and efflux. In the context of diabetes, regression of atherosclerosis is impaired. We proposed that changes in glucose levels modulate LXR-dependent gene expression. Using a mouse macrophage cell line (RAW 264.7) and primary bone marrow derived macrophages (BMDMs) cultured in normal or diabetes relevant high glucose conditions we found that high glucose inhibits the LXR-dependent expression of ATP-binding cassette transporter A1 (ABCA1), but not ABCG1. To probe for this mechanism, we surveyed the expression of a host of chromatin-modifying enzymes and found that Protein Arginine Methyltransferase 2 (PRMT2) was reduced in high compared to normal glucose conditions. Importantly, ABCA1 expression and ABCA1-mediated cholesterol efflux were reduced in Prmt2-/- compared to wild type BMDMs. Monocytes from diabetic mice also showed decreased expression of Prmt2 compared to non-diabetic counterparts. Thus, PRMT2 represents a glucose-sensitive factor that plays a role in LXR-mediated ABCA1-dependent cholesterol efflux and lends insight to the presence of increased atherosclerosis in diabetic patients.</p>',
'date' => '2015-08-19',
'pmid' => 'http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135218',
'doi' => '10.1371/journal.pone.0135218',
'modified' => '2015-11-10 13:43:13',
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'name' => 'Sensitive detection of chromatin-altering polymorphisms reveals autoimmune disease mechanisms.',
'authors' => 'Del Rosario RC, Poschmann J, Rouam SL, Png E, Khor CC, Hibberd ML, Prabhakar S',
'description' => 'Most disease associations detected by genome-wide association studies (GWAS) lie outside coding genes, but very few have been mapped to causal regulatory variants. Here, we present a method for detecting regulatory quantitative trait loci (QTLs) that does not require genotyping or whole-genome sequencing. The method combines deep, long-read chromatin immunoprecipitation-sequencing (ChIP-seq) with a statistical test that simultaneously scores peak height correlation and allelic imbalance: the genotype-independent signal correlation and imbalance (G-SCI) test. We performed histone acetylation ChIP-seq on 57 human lymphoblastoid cell lines and used the resulting reads to call 500,066 single-nucleotide polymorphisms de novo within regulatory elements. The G-SCI test annotated 8,764 of these as histone acetylation QTLs (haQTLs)-an order of magnitude larger than the set of candidates detected by expression QTL analysis. Lymphoblastoid haQTLs were highly predictive of autoimmune disease mechanisms. Thus, our method facilitates large-scale regulatory variant detection in any moderately sized cohort for which functional profiling data can be generated, thereby simplifying identification of causal variants within GWAS loci.',
'date' => '2015-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25799442',
'doi' => '',
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'name' => 'Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus, Osmeridae), an anadromous Pacific smelt.',
'authors' => 'Candy JR, Campbell NR, Grinnell MH, Beacham TD, Larson WA, Narum SR',
'description' => 'Twelve eulachon (Thaleichthys pacificus, Osmeridae) populations ranging from Cook Inlet, Alaska and along the west coast of North America to the Columbia River were examined by restriction-site-associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high geneflow species. A total of 4104 single-nucleotide polymorphisms (SNPs) were discovered across the genome, with 193 putatively adaptive SNPs as determined by FST outlier tests. Estimates of population structure in eulachon with the putatively adaptive SNPs were similar, but provided greater resolution of stocks compared with a putatively neutral panel of 3911 SNPs or previous estimates with 14 microsatellites. A cline of increasing measures of genetic diversity from south to north was found in the adaptive panel, but not in the neutral markers (SNPs or microsatellites). This may indicate divergent selective pressures in differing freshwater and marine environments between regional eulachon populations and that these adaptive diversity patterns not seen with neutral markers could be a consideration when determining genetic boundaries for conservation purposes. Estimates of effective population size (Ne ) were similar with the neutral SNP panel and microsatellites and may be utilized to monitor population status for eulachon where census sizes are difficult to obtain. Greater differentiation with the panel of putatively adaptive SNPs provided higher individual assignment accuracy compared to the neutral panel or microsatellites for stock identification purposes. This study presents the first SNPs that have been developed for eulachon, and analyses with these markers highlighted the importance of integrating genome-wide neutral and adaptive genetic variation for the applications of conservation and management.',
'date' => '2015-03-03',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25737187',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
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'name' => 'Silica nanoparticles induce oxidative stress, inflammation, and endothelial dysfunction in vitro via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling.',
'authors' => 'Caixia Guo,1,2 Yinye Xia,1,2 Piye Niu,1,2 Lizhen Jiang,1,2 Junchao Duan,1,2 Yang Yu,1,2 Xianqing Zhou,1,2 Yanbo Li,1,2 Zhiwei Sun1,2 ',
'description' => '<p><strong>Abstract:</strong><span> Despite the widespread application of silica nanoparticles (SiNPs) in industrial, commercial, and biomedical fields, their response to human cells has not been fully elucidated. Overall, little is known about the toxicological effects of SiNPs on the cardiovascular system. In this study, SiNPs with a 58 nm diameter were used to study their interaction with human umbilical vein endothelial cells (HUVECs). Dose- and time-dependent decrease in cell viability and damage on cell plasma-membrane integrity showed the cytotoxic potential of the SiNPs. SiNPs were found to induce oxidative stress, as evidenced by the significant elevation of reactive oxygen species generation and malondialdehyde production and downregulated activity in glutathione peroxidase. SiNPs also stimulated release of cytoprotective nitric oxide (NO) and upregulated inducible nitric oxide synthase (NOS) messenger ribonucleic acid, while downregulating endothelial NOS and ET-1 messenger ribonucleic acid, suggesting that SiNPs disturbed the NO/NOS system. SiNP-induced oxidative stress and NO/NOS imbalance resulted in endothelial dysfunction. SiNPs induced inflammation characterized by the upregulation of key inflammatory mediators, including IL-1β, IL-6, IL-8, TNFα, ICAM-1, VCAM-1, and MCP-1. In addition, SiNPs triggered the activation of the Nrf2-mediated antioxidant system, as evidenced by the induction of nuclear factor-κB and MAPK pathway activation. Our findings demonstrated that SiNPs could induce oxidative stress, inflammation, and NO/NOS system imbalance, and eventually lead to endothelial dysfunction via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling. This study indicated a potential deleterious effect of SiNPs on the vascular endothelium, which warrants more careful assessment of SiNPs before their application. </span></p>',
'date' => '2015-02-20',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25759575',
'doi' => '10.2147/IJN.S76114',
'modified' => '2016-11-29 10:33:26',
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'name' => 'Step-by-step preparation of proteins for mass spectrometric analysis.',
'authors' => 'Franz T, Li X.',
'description' => 'Nowadays, identification of proteins from biological samples by mass spectrometry is widely used. In principle there are two scenarios. Proteins are pre-fractionated in some way, e.g. by gel electrophoresis or are analyzed as complex mixture (shot gun). Shot gun proteomics became recently more popular, because of technological developments on the mass spectrometer side which allows now the identification of several thousand proteins from complex biological matrix. However, in many cases it is still useful to separate proteins first in a gel. But not only mass spectrometer technology made progress. This is also true for the sample preparation. Recently, protocols and techniques were developed which make the analysis of starting material in the low microgram range possible and also simplify the whole procedure. Detailed protocols will be described allowing also inexperienced beginners to get good results. ',
'date' => '0000-00-00',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/25820726',
'doi' => '',
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'name' => 'Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours',
'authors' => 'Bujko M, Kober P, Statkiewicz M, Mikula M, Ligaj M, Zwierzchowski L, Ostrowski J, Siedlecki JA',
'description' => 'Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). Results indicate that μ-protocadherin downregulation occurs during early stages of tumourigenesis and progression into the adenoma-carcinoma sequence. Epigenetic mechanisms are involved in this silencing.',
'date' => '0000-00-00',
'pmid' => 'http://www.hindawi.com/journals/grp/2015/317093/',
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'description' => '<p>We use the <strong>Bioruptor</strong> sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The <strong>H3K4me3 antibody</strong> is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.</p>
<p>Diagenode provides not only very good products for research but also an excellent customer support.</p>',
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<p>Diagenode provides not only very good products for research but also an excellent customer support.</p><cite>Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany</cite></blockquote>
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'4950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 115V',
'B02010003',
'4950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="water-cooler-115v-1-unit" data-reveal-id="cartModal-1814" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Water cooler, 115V</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/water-cooler-230v-1-unit"><img src="/img/product/shearing_technologies/B02010003%20_water_cooler.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02010002</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1813" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1813" id="CartAdd/1813Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1813" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Water cooler, 230V</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 230V',
'B02010002',
'4950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 230V',
'B02010002',
'4950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="water-cooler-230v-1-unit" data-reveal-id="cartModal-1813" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Water cooler, 230V</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/single-cycle-valve-for-water-cooler-1-unit"><img src="/img/product/shearing_technologies/B02020004%20_single_cycle_valve.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02020005</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1816" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1816" id="CartAdd/1816Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1816" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Single Cycle Valve for Water Cooler</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="single-cycle-valve-for-water-cooler-1-unit" data-reveal-id="cartModal-1816" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Single Cycle Valve for Water Cooler</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/metallic-soundproof-box-1-unit"><img src="/img/product/shearing_technologies/B01200001%20_soundproof_box.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200001</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1790" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1790" id="CartAdd/1790Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1790" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Metallic soundproof box</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="metallic-soundproof-box-1-unit" data-reveal-id="cartModal-1790" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Metallic soundproof box</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-ml-bioruptor-microtubes-500-tubes"><img src="/img/product/shearing_technologies/C30010013_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010013</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2627" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2627" id="CartAdd/2627Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2627" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5 ml Bioruptor<sup>®</sup> Plus Microtubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-ml-bioruptor-microtubes-500-tubes" data-reveal-id="cartModal-2627" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5 ml Bioruptor® Plus Microtubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010009</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Plus TPX tubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/rna-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000010</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2616" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2616" id="CartAdd/2616Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2616" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> RNA Extraction kit for Bioruptor Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
'165',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
'165',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="rna-extraction-kit-50-rxns" data-reveal-id="cartModal-2616" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">RNA Extraction kit for Bioruptor Plus</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/protein-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000020</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2617" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2617" id="CartAdd/2617Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2617" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction kit for Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-kit-50-rxns" data-reveal-id="cartModal-2617" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/protein-extraction-beads-20-g"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000021</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2618" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2618" id="CartAdd/2618Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2618" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction beads</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads',
'C20000021',
'100',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads',
'C20000021',
'100',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-beads-20-g" data-reveal-id="cartModal-2618" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Protein Extraction beads</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/Bioruptor-Plus-DNA-QC-kit"><img src="/img/product/kits/dnaqckit.jpg" alt="dna qc kit" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C40010001</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2835" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<h6 style="height:60px">Bioruptor® Plus DNA QC kit</h6>
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<p style="text-align: left;"><strong>SDS concentration</strong></p>
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<p style="text-align: center;">< 0.1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
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<td style="text-align: center;">
<p style="text-align: center;">1%</p>
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<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<td style="text-align: center;">
<p style="text-align: center;">No</p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
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<p style="text-align: center;">100 million cells</p>
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<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
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<p style="text-align: center;">up to 25 g of tissue</p>
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<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
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<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
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<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
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<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
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<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
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<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
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<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
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<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
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<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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$meta_title = 'Bioruptor® Plus sonication device'
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<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
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<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
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<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<tbody>
<tr>
<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
</tr>
<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
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<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
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<tr>
<td>Safety classification</td>
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<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
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<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
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<tr>
<td>Refrigerant</td>
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<tr>
<td>Refrigerant quantity</td>
<td>0.04 kg</td>
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<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
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<tr>
<td>max. delivery</td>
<td>20 l/min</td>
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<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
</tr>
<tr>
<td>max. delivery (suction)</td>
<td>17 l/min</td>
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<tr>
<td>Pump connection</td>
<td>G 1/4" female thread</td>
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<tr>
<td>min. filling capacity</td>
<td>1.4 l</td>
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<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
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<tr>
<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
</tr>
<tr>
<td>Net weight</td>
<td>23 kg</td>
</tr>
<tr>
<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
</tr>
<tr>
<td>max. current</td>
<td>2.8 A</td>
</tr>
<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
</tr>
<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
</tr>
<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
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'price_JPY' => '759750',
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<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
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<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
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<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
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<tr>
<td>Safety classification</td>
<td>Class I/NFL</td>
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<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
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<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
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<tr>
<td>Refrigerant</td>
<td>R290</td>
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<tr>
<td>Refrigerant quantity</td>
<td>0.04 kg</td>
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<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
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<tr>
<td>max. delivery</td>
<td>20 l/min</td>
</tr>
<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
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<tr>
<td>max. delivery (suction)</td>
<td>17 l/min</td>
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<tr>
<td>Pump connection</td>
<td>G 1/4" female thread</td>
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<td>min. filling capacity</td>
<td>1.4 l</td>
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<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
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<tr>
<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
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<tr>
<td>Net weight</td>
<td>23 kg</td>
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<tr>
<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
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<tr>
<td>max. current</td>
<td>2.8 A</td>
</tr>
<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
</tr>
<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
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<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
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'price_GBP' => '4300',
'price_JPY' => '759750',
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'price_AUD' => '12375',
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'name' => 'Single Cycle Valve for Water Cooler',
'description' => '<p><span>The Single Cycle Valve for the Bioruptor® controls the water flow between the Bioruptor® Pico (B01060001) / Bioruptor® Plus (UCD-300) and the water cooler. Cold water cycles into the Bioruptor® sonication bath only during the off cycle (no sonication) and is stopped during the on cycle (sonication). This reduces any water flow disturbance in the tank and allows for more efficient sonication of samples.</span></p>
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<li>Compatible with Bioruptor® Pico and Bioruptor® Plus</li>
<li>Increases shearing efficiency</li>
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'format' => '1 unit',
'catalog_number' => 'B02020005',
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'sf_code' => 'B02020005-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1050',
'price_USD' => '950',
'price_GBP' => '900',
'price_JPY' => '164480',
'price_CNY' => '',
'price_AUD' => '2375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
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'slug' => 'single-cycle-valve-for-water-cooler-1-unit',
'meta_title' => 'Single Cycle Valve for Water Cooler',
'meta_keywords' => '',
'meta_description' => 'Single Cycle Valve for Water Cooler',
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'description' => '<p><span>This metallic soundproof box has been designed for a highly efficient acoustic insulation of the Bioruptor® sonication bath. This box is only compatible with Bioruptor® standard and Bioruptor® Plus. Can be stored at room temperature or in a cold room (4°C).</span></p>',
'label1' => 'Characteristics',
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<li>Compatible with Bioruptor® standard and Bioruptor® Plus</li>
<li>Very efficient acoustic insulation of the Bioruptor waterbath.</li>
<li>Dimensions: 35(W) x 30(D) x 52(H) mm</li>
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<p><strong>Dimensions :</strong><br />Width : 35 cm<br />Depth : 35 cm<br />Height : 52 cm</p>',
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'format' => '1 unit',
'catalog_number' => 'B01200001',
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'sf_code' => 'B01200001-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1550',
'price_USD' => '1450',
'price_GBP' => '1400',
'price_JPY' => '242810',
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'price_AUD' => '3625',
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'meta_title' => 'Metallic soundproof box',
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'meta_description' => 'Metallic soundproof box',
'modified' => '2019-10-20 13:42:38',
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'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
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'info1' => '',
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'label3' => '',
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'format' => '500 tubes',
'catalog_number' => 'C30010013',
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'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
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'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
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'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
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'format' => '1 pack',
'catalog_number' => 'B01200043',
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'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
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'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
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'name' => '15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'description' => '<p><span>TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultrasound to the sample. TPX® tubes have been validated for use on the Bioruptors® Standard and Bioruptors® Plus. Recommended sample volume: 500 μl - 2 ml.</span></p>',
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'format' => '50 pc',
'catalog_number' => 'C30010009',
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'type' => 'ACC',
'search_order' => '01-Accessory',
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'price_USD' => '120',
'price_GBP' => '95',
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'price_AUD' => '300',
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'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s </span>Bioruptor<sup>®</sup> Plus<span> used in combination with the Protein Extraction kit ensures efficient disruption, homogenization and high yields of proteins from tissues. Tubes are already pre-filled with the protein extraction beads which have been designed to enhance the protein extraction process.</span></p>',
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<li>Fast and simple</li>
<li>No contamination between samples</li>
<li>Efficient</li>
<li>Gentle processing</li>
<li>Reproducible</li>
<li>Temperature controlled</li>
<li>Multiplexing capability</li>
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'type' => 'REF',
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'price_JPY' => '14880',
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<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
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'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. <strong>Diagenode's Bioruptor</strong><sup>® </sup><strong>Plus</strong> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.</div>
<div class="large-12 columns"></div>
<div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/pro-rna-extract-fig3a.png" /></div>
<div class="small-6 medium-6 large-6 columns text-center end small-offset-3"><small><strong>Efficient extraction of pure RNA with high RIN. </strong><br /><span>Total RNA profiles from mouse brain. Tissue was disrupted with Bioruptor<sup>®</sup> Plus as described in the protocol and analyzed on BioAnalyzer (Agilent). Note that small RNAs are present in all profiles indicating that the RNA is largely intact.</span></small></div>
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'meta_title' => 'RNA extraction for Next Generation Sequencing using Diagenode's Bioruptor® sonication device | Diagenode',
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div>
</div>
<p></p>
<div class="row">
<div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" />
<p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p>
</div>
<div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" />
<p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p>
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<div class="large-12 columns">Diagenode's high yields FFPE DNA extraction using Bioruptor<sup><span>®</span></sup> is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by traditional methods or can be sheared with the Bioruptor<sup>®</sup> Pico ultrasonicator for downstream NGS library prep using the MicroPlex Library Preparation Kit.</div>
<div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/ffpe_workflow.png" /></div>
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<div class="small-12 medium-12 large-12 columns"><br />
<p><span>Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</span></p>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/TAB-BR-comparaison.pdf" target="_blank"><img src="https://www.diagenode.com/img/bouton-comparaison.png" /></a></p>
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<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">Technology explained</h2>
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<h2 style="text-align: center;">Reproductibility is our priority</h2>
</div>
</div>
<div><img src="https://www.diagenode.com/img/shearing/reproductibility.png" alt="reproductibility" />
<p class="bottom_note"></p>
</div>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;">An affordable instrument for wide range of applications</h2>
</div>
</div>
<p style="text-align: center;">Designed for any researchers, the Bioruptor gives the user the right level of flexibility.</p>
<table style="width: 972px;">
<tbody>
<tr style="height: 56px;">
<th style="width: 380px; height: 56px;"></th>
<th class="text-center" style="width: 126px; height: 56px;">Bioruptor</th>
<th class="text-center" style="width: 141px; height: 56px;">Cup Horn Sonicators</th>
<th class="text-center" style="width: 156px; height: 56px;">Focused <br />ultra-sonicators</th>
<th class="text-center" style="width: 155px; height: 56px;">Multi Sample Sonicator</th>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Instrument pricing</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Consumables pricing</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Range of applications</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Scalable and sample volume flexibility</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
<tr style="height: 38px;">
<td style="width: 380px; height: 38px;">Throughput</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
</tbody>
</table>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">Bioruptor ultrasonication for best results in:</h2>
<p><b><span>✓ Chromatin shearing</span><span> </span><span style="font-weight: 400;">- Industry leader in accurate and tight fragment ranges</span></b></p>
<p><b><span>✓ DNA shearing</span><span> </span><span style="font-weight: 400;">- Excellent results for optimal fragment lengths in NGS library prep</span></b></p>
<p><b><span>✓<span> </span></span>Protein aggregation studies </b><span style="font-weight: 400;">- Standardizing seeding with the robust Bioruptor.<br /></span><i><span style="font-weight: 400;">Read the app note by Dr. Kelvin Luk at the University of Pennsylvania </span></i><a href="https://www.diagenode.com/en/documents/standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease" style="color: #13b29c;"><i><span style="font-weight: 400;">“Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>3D genome analysis with Hi-C</b><span style="font-weight: 400;"> - Preparing chromatin libraries with high-quality sonication.<br /></span><i><span style="font-weight: 400;">Read the app note, “</span></i><a href="https://www.diagenode.com/en/documents/applicationnote-arima-low-input" style="color: #13b29c;"><i><span style="font-weight: 400;">Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>Mass spectrometry</b> <b>and increasing protein identification</b><span style="font-weight: 400;">- Sample preparation using Preomics iST and Bioruptor sonication.<br /></span><i><span style="font-weight: 400;">Read the app note “</span></i><a href="https://www.diagenode.com/en/documents/wp-ist-adaptators" style="color: #13b29c;"><i><span style="font-weight: 400;">Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder”</span></i></a></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>Cell lysis, liposome prep, protein extraction, RNA extraction and more</b></span></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>CUT&RUN –Sonication of input DNA (for enrichment comparison) for NGS</b></span></p>
</div>
</div>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
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<p><span>Disruption of bacterial cells is required for releasing biological molecules from within the cells for various biological applications. Ultrasound technology has been widely used for such cell lysis applications. Ultrasonic disruption is based on cavitation, the creation of cavities in cell suspension. These cavities or small bubbles of dissolved gases or vapors arise from the alteration of pressure in liquids. The bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming leading to mechanical stress that effectively lyses cells. Diagenode’s Bioruptor</span><span><sup>®</sup> </span><span>sonicaton device uses state-of-the-art ultrasound technology to disrupt cells for biological, chemical, pharmaceutical, and industrial applications. </span></p>
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'id' => '61',
'name' => 'Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis',
'description' => '<p>Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck Institute for Biochemistry, Martinsried, Germany are using the Bioruptor<span>® </span>for their research using C. elegans, a powerful metazoan model system to study and understand fundamental problems in biology. Traditionally C. elegans research has focused mainly on genetics. However, recent studies suggest that C. elegans can also be a facile system for biochemistry. Tagged proteins can be expressed in vivo and interacting partners can be identified using various biochemical approaches. In addition, protein networks and pathways can be identified by combining genetic screens with biochemistry.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/protocols/mass_spectrometry -Celegans-protocol.pdf',
'slug' => 'mass_spectrometry -Celegans-protocol',
'meta_keywords' => 'mass_spectrometry -Celegans-protocol',
'meta_description' => 'mass_spectrometry -Celegans-protocol',
'modified' => '2016-01-15 12:07:23',
'created' => '2016-01-15 11:55:19',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 9 => array(
'id' => '62',
'name' => 'Mass-Spectrometry: procedure for shotgun proteomics',
'description' => '<p>Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in addition to degradation and removal of unwanted components like nucleic acids. Therefore, it requires mechanical breakdown accompanied by denaturing agents and heating of the samples. Matthias Mann and his team from the Department of Proteomics and Signal Transduction at the Max Plank Institute of Biochemistry developed and employ highly efficient protocols for protein isolation in conjunction with Bioruptor to perform quantitative proteomics.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/protocols/bioruptor-mass_spectometry-protocol.pdf',
'slug' => 'bioruptor-mass_spectometry-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-02-05 14:22:56',
'created' => '2016-02-05 14:22:56',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 10 => array(
'id' => '23',
'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus',
'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>',
'image_id' => '213',
'type' => 'Protocol',
'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf',
'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:40:14',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 11 => array(
'id' => '26',
'name' => 'RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit',
'description' => '<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disruption releases RNA, while homogenization reduces sample viscosity to facilitate RNA purification. Diagenode’s Bioruptor</span><span>® </span><span>Sonicator uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues in one step. Diagenode’s RNA extraction reagent (included in the RNA extraction kit) is used as sonication medium and maintains the integrity of RNA while disrupting cells and dissolving cell components. </span></p>',
'image_id' => '216',
'type' => 'Protocol',
'url' => 'files/protocols/RNA_Extraction_from_Tissue_with_Bioruptor_Standard_Plus_protocol.pdf',
'slug' => 'rna-extraction-from-tissue-with-bioruptor-standard-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:45:55',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 12 => array(
'id' => '43',
'name' => 'TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®',
'description' => '<p><span>The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a native environment. Molecular complexes can then be isolated and used for various applications for the identification of partners. Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes combined the TAP tagging with the Bioruptor</span><span>®</span><span>. This combination is essential for the efficient purification of a chromatin protein. </span></p>',
'image_id' => null,
'type' => 'Protocol',
'url' => 'files/protocols/TAP-TAG-procedure-for-bioruptor.pdf',
'slug' => 'tap-tag-procedure-for-bioruptor',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:40',
'created' => '2015-08-10 10:50:46',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 13 => array(
'id' => '24',
'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®',
'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>',
'image_id' => '220',
'type' => 'Protocol',
'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf',
'slug' => 'western-blot-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:39:29',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4454',
'name' => 'Histone lysine demethylase inhibition reprograms prostate cancermetabolism and mechanics.',
'authors' => 'Chianese Ugo and Papulino Chiara and Passaro Eugenia andEvers Tom Mj and Babaei Mehrad and Toraldo Antonella andDe Marchi Tommaso and Niméus Emma and Carafa Vincenzo andNicoletti Maria Maddalena and Del Gaudio Nunzio andIaccarino Nunzia an',
'description' => '<p>OBJECTIVE: Aberrant activity of androgen receptor (AR) is the primary cause underlying development and progression of prostate cancer (PCa) and castration-resistant PCa (CRPC). Androgen signaling regulates gene transcription and lipid metabolism, facilitating tumor growth and therapy resistance in early and advanced PCa. Although direct AR signaling inhibitors exist, AR expression and function can also be epigenetically regulated. Specifically, lysine (K)-specific demethylases (KDMs), which are often overexpressed in PCa and CRPC phenotypes, regulate the AR transcriptional program. METHODS: We investigated LSD1/UTX inhibition, two KDMs, in PCa and CRPC using a multi-omics approach. We first performed a mitochondrial stress test to evaluate respiratory capacity after treatment with MC3324, a dual KDM-inhibitor, and then carried out lipidomic, proteomic, and metabolic analyses. We also investigated mechanical cellular properties with acoustic force spectroscopy. RESULTS: MC3324 induced a global increase in H3K4me2 and H3K27me3 accompanied by significant growth arrest and apoptosis in androgen-responsive and -unresponsive PCa systems. LSD1/UTX inhibition downregulated AR at both transcriptional and non-transcriptional level, showing cancer selectivity, indicating its potential use in resistance to androgen deprivation therapy. Since MC3324 impaired metabolic activity, by modifying the protein and lipid content in PCa and CRPC cell lines. Epigenetic inhibition of LSD1/UTX disrupted mitochondrial ATP production and mediated lipid plasticity, which affected the phosphocholine class, an important structural element for the cell membrane in PCa and CRPC associated with changes in physical and mechanical properties of cancer cells. CONCLUSIONS: Our data suggest a network in which epigenetics, hormone signaling, metabolite availability, lipid content, and mechano-metabolic process are closely related. This network may be able to identify additional hotspots for pharmacological intervention and underscores the key role of KDM-mediated epigenetic modulation in PCa and CRPC.</p>',
'date' => '2022-08-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35944897',
'doi' => '10.1016/j.molmet.2022.101561',
'modified' => '2022-10-21 09:37:56',
'created' => '2022-09-28 09:53:13',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4214',
'name' => 'Comprehensive characterization of the epigenetic landscape in Multiple Myeloma',
'authors' => 'Elina Alaterre et al.',
'description' => '<p>Background: Human multiple myeloma (MM) cell lines (HMCLs) have been widely used to understand the<br />molecular processes that drive MM biology. Epigenetic modifications are involved in MM development,<br />progression, and drug resistance. A comprehensive characterization of the epigenetic landscape of MM would<br />advance our understanding of MM pathophysiology and may attempt to identify new therapeutic targets.<br />Methods: We performed chromatin immunoprecipitation sequencing to analyze histone mark changes<br />(H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3 and H3K36me3) on 16 HMCLs.<br />Results: Differential analysis of histone modification profiles highlighted links between histone modifications<br />and cytogenetic abnormalities or recurrent mutations. Using histone modifications associated to enhancer<br />regions, we identified super-enhancers (SE) associated with genes involved in MM biology. We also identified<br />promoters of genes enriched in H3K9me3 and H3K27me3 repressive marks associated to potential tumor<br />suppressor functions. The prognostic value of genes associated with repressive domains and SE was used to<br />build two distinct scores identifying high-risk MM patients in two independent cohorts (CoMMpass cohort; n =<br />674 and Montpellier cohort; n = 69). Finally, we explored H3K4me3 marks comparing drug-resistant and<br />-sensitive HMCLs to identify regions involved in drug resistance. From these data, we developed epigenetic<br />biomarkers based on the H3K4me3 modification predicting MM cell response to lenalidomide and histone<br />deacetylase inhibitors (HDACi).<br />Conclusions: The epigenetic landscape of MM cells represents a unique resource for future biological studies.<br />Furthermore, risk-scores based on SE and repressive regions together with epigenetic biomarkers of drug<br />response could represent new tools for precision medicine in MM.</p>',
'date' => '2022-01-16',
'pmid' => 'https://www.thno.org/v12p1715',
'doi' => '10.7150/thno.54453',
'modified' => '2022-01-27 13:17:28',
'created' => '2022-01-27 13:14:17',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4164',
'name' => 'Chromatin dysregulation associated with NSD1 mutation in head and necksquamous cell carcinoma.',
'authors' => 'Farhangdoost, Nargess et al. ',
'description' => '<p>Chromatin dysregulation has emerged as an important mechanism of oncogenesis. To develop targeted treatments, it is important to understand the transcriptomic consequences of mutations in chromatin modifier genes. Recently, mutations in the histone methyltransferase gene nuclear receptor binding SET domain protein 1 (NSD1) have been identified in a subset of common and deadly head and neck squamous cell carcinomas (HNSCCs). Here, we use genome-wide approaches and genome editing to dissect the downstream effects of loss of NSD1 in HNSCC. We demonstrate that NSD1 mutations are responsible for loss of intergenic H3K36me2 domains, followed by loss of DNA methylation and gain of H3K27me3 in the affected genomic regions. In addition, those regions are enriched in cis-regulatory elements, and subsequent loss of H3K27ac correlates with reduced expression of their target genes. Our analysis identifies genes and pathways affected by the loss of NSD1 and paves the way to further understanding the interplay among chromatin modifications in cancer.</p>',
'date' => '2021-02-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33626351',
'doi' => '10.1016/j.celrep.2021.108769',
'modified' => '2021-12-21 15:35:45',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3396',
'name' => 'The Itaconate Pathway Is a Central Regulatory Node Linking Innate Immune Tolerance and Trained Immunity',
'authors' => 'Domínguez-Andrés Jorge, Novakovic Boris, Li Yang, Scicluna Brendon P., Gresnigt Mark S., Arts Rob J.W., Oosting Marije, Moorlag Simone J.C.F.M., Groh Laszlo A., Zwaag Jelle, Koch Rebecca M., ter Horst Rob, Joosten Leo A.B., Wijmenga Cisca, Michelucci Ales',
'description' => '<p>Sepsis involves simultaneous hyperactivation of the immune system and immune paralysis, leading to both organ dysfunction and increased susceptibility to secondary infections. Acute activation of myeloid cells induced itaconate synthesis, which subsequently mediated innate immune tolerance in human monocytes. In contrast, induction of trained immunity by b-glucan counteracted tolerance induced in a model of human endotoxemia by inhibiting the expression of immune-responsive gene 1 (IRG1), the enzyme that controls itaconate synthesis. b-Glucan also increased the expression of succinate dehydrogenase (SDH), contributing to the integrity of the TCA cycle and leading to an enhanced innate immune response after secondary stimulation. The role of itaconate was further validated by IRG1 and SDH polymorphisms that modulate induction of tolerance and trained immunity in human monocytes. These data demonstrate the importance of the IRG1-itaconateSDH axis in the development of immune tolerance and training and highlight the potential of b-glucaninduced trained immunity to revert immunoparalysis.</p>',
'date' => '2018-10-01',
'pmid' => 'http://www.pubmed.gov/30293776',
'doi' => '10.1016/j.cmet.2018.09.003',
'modified' => '2018-11-22 15:18:30',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3377',
'name' => 'Interplay of cell–cell contacts and RhoA/MRTF‐A signaling regulates cardiomyocyte identity',
'authors' => 'Dorn et al',
'description' => '<p><span>Cell–cell and cell–matrix interactions guide organ development and homeostasis by controlling lineage specification and maintenance, but the underlying molecular principles are largely unknown. Here, we show that in human developing cardiomyocytes cell–cell contacts at the intercalated disk connect to remodeling of the actin cytoskeleton by regulating the RhoA‐</span><span class="styled-content fixed-case">ROCK</span><span><span> </span>signaling to maintain an active<span> </span></span><span class="styled-content fixed-case">MRTF</span><span>/</span><span class="styled-content fixed-case">SRF</span><span><span> </span>transcriptional program essential for cardiomyocyte identity. Genetic perturbation of this mechanosensory pathway activates an ectopic fat gene program during cardiomyocyte differentiation, which ultimately primes the cells to switch to the brown/beige adipocyte lineage in response to adipogenesis‐inducing signals. We also demonstrate by<span> </span></span><em>in vivo</em><span><span> </span>fate mapping and clonal analysis of cardiac progenitors that cardiac fat and a subset of cardiac muscle arise from a common precursor expressing Isl1 and Wt1 during heart development, suggesting related mechanisms of determination between the two lineages.</span></p>',
'date' => '2018-05-15',
'pmid' => 'http://emboj.embopress.org/content/early/2018/05/15/embj.201798133',
'doi' => '10.15252/embj.201798133',
'modified' => '2018-05-22 22:50:53',
'created' => '2018-05-22 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3368',
'name' => 'Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity',
'authors' => 'Darya A. Haas, Arno Meiler, Katharina Geiger, Carola Vogt, Ellen Preuss, Georg Kochs, Andreas Pichlmair',
'description' => '<p><span>Viruses have evolved a plethora of mechanisms to target host antiviral responses. Here, we propose a yet uncharacterized mechanism of immune regulation by the orthomyxovirus Thogoto virus (THOV) ML protein through engaging general transcription factor TFIIB. ML generates a TFIIB depleted nuclear environment by re-localizing it into the cytoplasm. Although a broad effect on gene expression would be anticipated, ML expression, delivery of an ML-derived functional domain or experimental depletion of TFIIB only leads to altered expression of a limited number of genes. Our data indicate that TFIIB is critically important for the </span><em>de novo</em><span>recruitment of Pol II to promoter start sites and that TFIIB may not be required for regulated gene expression from paused promoters. Since many immune genes require<span> </span></span><em>de novo</em><span>recruitment of Pol II, targeting of TFIIB by THOV represents a neat mechanism to affect immune responses while keeping other cellular transcriptional activities intact. Thus, interference with TFIIB activity may be a favourable site for therapeutic intervention to control undesirable inflammation.</span></p>',
'date' => '2018-04-30',
'pmid' => 'http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006980',
'doi' => '10.1371/journal.ppat.1006980',
'modified' => '2018-05-09 08:55:23',
'created' => '2018-05-09 08:55:23',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3369',
'name' => 'HMGB2 Loss upon Senescence Entry Disrupts Genomic Organization and Induces CTCF Clustering across Cell Types',
'authors' => 'Zirkel et. al.',
'description' => '<p><span>Processes like cellular senescence are characterized by complex events giving rise to heterogeneous cell populations. However, the early molecular events driving this cascade remain elusive. We hypothesized that senescence entry is triggered by an early disruption of the cells’ three-dimensional (3D) <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/genome-organization" title="Learn more about Genome Organization">genome organization</a>. To test this, we combined Hi-C, single-cell and population <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transcriptome" title="Learn more about Transcriptome">transcriptomics</a>, imaging, and </span><em>in silico</em><span>modeling of three distinct cells types entering senescence. Genes involved in DNA conformation maintenance are suppressed upon senescence entry across all cell types. We show that nuclear depletion of the abundant<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/hmgb2" title="Learn more about HMGB2">HMGB2</a><span> </span>protein occurs early on the path to senescence and coincides with the dramatic spatial clustering of CT</span><em>CF.</em><span>Knocking down<span> </span></span><em>HMGB2</em><span><span> </span>suffices for senescence-induced<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ctcf" title="Learn more about CTCF">CTCF</a><span> </span>clustering and for loop reshuffling, while ectopically expressing<span> </span></span><em>HMGB2</em><span><span> </span>rescues these effects. Our data suggest that HMGB2-mediated genomic reorganization constitutes a<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/primer-molecular-biology" title="Learn more about Primer (molecular biology)">primer</a><span> </span>for the ensuing senescent program.</span></p>',
'date' => '2018-04-26',
'pmid' => 'https://www.sciencedirect.com/science/article/pii/S1097276518302338',
'doi' => '10.1016/j.molcel.2018.03.030',
'modified' => '2018-05-09 22:50:53',
'created' => '2018-05-09 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3337',
'name' => 'Widespread bacterial protein histidine phosphorylation revealed by mass spectrometry-based proteomics',
'authors' => 'Clement M Potel, Miao-Hsia Lin, Albert J R Heck, Simone Lemeer',
'description' => '<p>For decades, major difficulties in analyzing histidine phosphorylation have limited the study of phosphohistidine signaling. Here we report a method revealing widespread and abundant protein histidine phosphorylation in <i>Escherichia coli</i>. We generated an extensive <i>E. coli</i> phosphoproteome data set, in which a remarkably high percentage (<span class="stix"><span class="stix">∼</span></span>10%) of phosphorylation sites are phosphohistidine sites. This resource should help enable a better understanding of the biological function of histidine phosphorylation.</p>',
'date' => '2018-01-31',
'pmid' => 'https://www.nature.com/articles/nmeth.4580',
'doi' => '10.1038/nmeth.4580',
'modified' => '2018-02-11 02:37:05',
'created' => '2018-02-11 02:37:05',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 8 => array(
'id' => '3214',
'name' => 'MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1',
'authors' => 'Ann-Christin Hau, Britta Moyo Grebbin, Zsuzsa Agoston, Marie Anders-Maurer, Tamara Müller, Anja Groß, Jasmine Kolb, Julian D. Langer, Claudia Döring, Dorothea Schulte',
'description' => '<p><span>Pre–B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone–derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes: In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron-specific gene </span><em>doublecortin (Dcx)</em><span>. Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.</span></p>',
'date' => '2017-07-24',
'pmid' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'doi' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'modified' => '2017-08-29 11:17:03',
'created' => '2017-07-29 07:39:31',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 9 => array(
'id' => '3006',
'name' => 'reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells',
'authors' => 'Kinkley S et al.',
'description' => '<p>The combinatorial action of co-localizing chromatin modifications and regulators determines chromatin structure and function. However, identifying co-localizing chromatin features in a high-throughput manner remains a technical challenge. Here we describe a novel reChIP-seq approach and tailored bioinformatic analysis tool, normR that allows for the sequential enrichment and detection of co-localizing DNA-associated proteins in an unbiased and genome-wide manner. We illustrate the utility of the reChIP-seq method and normR by identifying H3K4me3 or H3K27me3 bivalently modified nucleosomes in primary human CD4(+) memory T cells. We unravel widespread bivalency at hypomethylated CpG-islands coinciding with inactive promoters of developmental regulators. reChIP-seq additionally uncovered heterogeneous bivalency in the population, which was undetectable by intersecting H3K4me3 and H3K27me3 ChIP-seq tracks. Finally, we provide evidence that bivalency is established and stabilized by an interplay between the genome and epigenome. Our reChIP-seq approach augments conventional ChIP-seq and is broadly applicable to unravel combinatorial modes of action.</p>',
'date' => '2016-08-17',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27530917',
'doi' => '',
'modified' => '2016-08-26 11:56:46',
'created' => '2016-08-26 11:38:15',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 10 => array(
'id' => '2981',
'name' => 'UMI-4C for quantitative and targeted chromosomal contact profiling',
'authors' => 'Omer Schwartzman, Zohar Mukamel, Noa Oded-Elkayam, Pedro Olivares-Chauvet, Yaniv Lubling, Gilad Landan, Shai Izraeli and Amos Tanay',
'description' => '<p>We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (umis) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.</p>',
'date' => '2016-07-04',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27376768',
'doi' => '10.1038/nmeth.3922',
'modified' => '2016-07-13 09:33:41',
'created' => '2016-07-13 09:32:29',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 11 => array(
'id' => '2911',
'name' => 'Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock',
'authors' => 'Yoon Sik Kim, Young-Beom Kim, Woong Bin Kim, Seung Won Lee, Seog Bae Oh, Hee-Chul Han, C. Justin LeeEmail author, Christopher S. Colwell and Yang In Kim',
'description' => '<h3>Background</h3>
<p>Recent evidence indicates that histamine, acting on histamine 1 receptor (H1R), resets the circadian clock in the mouse suprachiasmatic nucleus (SCN) by increasing intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) through the activation of Ca<sub>V</sub>1.3 L-type Ca<sup>2+</sup> channels and Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release from ryanodine receptor-mediated internal stores.</p>
<h3>Results</h3>
<p>In the current study, we explored the underlying mechanisms with various techniques including Ca<sup>2+</sup>- and Cl<sup>−</sup>-imaging and extracellular single-unit recording. Our hypothesis was that histamine causes Cl<sup>−</sup> efflux through cystic fibrosis transmembrane conductance regulator (CFTR) to elicit membrane depolarization needed for the activation of Ca<sub>V</sub>1.3 Ca<sup>2+</sup> channels in SCN neurons. We found that histamine elicited Cl<sup>−</sup> efflux and increased [Ca<sup>2+</sup>]<sub>i</sub> in dissociated mouse SCN cells. Both of these events were suppressed by bumetanide [Na<sup>+</sup>-K<sup>+</sup>-2Cl<sup>−</sup> cotransporter isotype 1 (NKCC1) blocker], CFTR<sub>inh</sub>-172 (CFTR inhibitor), gallein (G<sub>βγ</sub> protein inhibitor) and H89 [protein kinase A (PKA) inhibitor]. By itself, H1R activation with 2-pyridylethylamine increased the level of cAMP in the SCN and this regulation was prevented by gallein. Finally, histamine-evoked phase shifts of the circadian neural activity rhythm in the mouse SCN slice were blocked by bumetanide, CFTR<sub>inh</sub>-172, gallein or H89 and were not observed in NKCC1 or CFTR KO mice.</p>
<h3>Conclusions</h3>
<p>Taken together, these results indicate that histamine recruits the H1R-G<sub>βγ</sub>-cAMP/PKA pathway in the SCN neurons to activate Ca<sub>V</sub>1.3 channels through CFTR-mediated Cl<sup>−</sup> efflux and ultimately to phase-shift the circadian clock. This pathway and NKCC1 may well be potential targets for agents designed to treat problems resulting from the disturbance of the circadian system.</p>',
'date' => '2016-03-22',
'pmid' => 'http://molecularbrain.biomedcentral.com/articles/10.1186/s13041-016-0227-1',
'doi' => '10.1186/s13041-016-0227-1',
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'name' => 'Standardizing chromatin research: a simple and universal method for ChIP-seq',
'authors' => 'Laura Arrigoni, Andreas S. Richter, Emily Betancourt, Kerstin Bruder, Sarah Diehl, Thomas Manke and Ulrike Bönisch',
'description' => '<p><span>Here we demonstrate that harmonization of ChIP-seq workflows across cell types and conditions is possible when obtaining chromatin from properly isolated nuclei. We established an ultrasound-based nuclei extraction method (Nuclei Extraction by Sonication) that is highly effective across various organisms, cell types and cell numbers. The described method has the potential to replace complex cell-type-specific, but largely ineffective, nuclei isolation protocols. This article demonstrates protocol standardization using the Bioruptor shearing systems and the IP-Star Automation System for ChIP automation.</span></p>',
'date' => '2015-12-23',
'pmid' => 'http://pubmed.gov/26704968',
'doi' => '10.1093/nar/gkv1495',
'modified' => '2016-06-09 09:47:00',
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'name' => 'HNF1β drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC)',
'authors' => 'Filipa Lopes-Coelho, Sofia Gouveia-Fernandes, Luís G Gonçalves, Carolina Nunes, Inês Faustino, Fernanda Silva, Ana Félix, Sofia A Pereira, Jacinta Serpa',
'description' => '<p>Chemoresistance to platinum-based antineoplastic agents is a consistent feature among ovarian carcinomas; however, whereas high-grade serous carcinoma (OSC) acquires resistance during chemotherapy, ovarian clear cell carcinoma (OCCC) is intrinsically resistant. The main objective of this study was to explore, in vitro and in vivo, if hepatocyte nuclear factor 1β (HNF1β) and glutaminolysis contribute for the resistance of OCCC to carboplatin through the intrinsically increased GSH bioavailability. To disclose the role of HNF1β, experiments were also performed in an OSC cell line, which does not express HNF1β. Metabolic profiles, GSH quantification, HNF1β, and γ-glutamylcysteine ligase catalytic subunit (GCLC) and modifier subunit (GCLM) expression, cell cycle, and death were assessed in ES2 cell line (OCCC) and OVCAR3 cell line (OSC); HNF1β knockdown was performed in ES2 and murine model of subcutaneous and peritoneal OCCC tumors was established to test buthionine sulphoxamine (BSO), as a sensitizer to carboplatin. Glutaminolysis is activated in ES2 and OVCAR3, though ES2 exclusively synthesizes amino acids and GSH. ES2 cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 to carboplatin. HNF1β regulates the expression of GCLC, but not GCLM, and consequently GSH production in ES2. In vivo, BSO prior to carboplatin reduces dramatically subcutaneous tumor size and GSH levels, as well as peritoneal dissemination. Our study discloses HNF1β as the mediator of intrinsic OCCC chemoresistance and sheds a light to re-explore a cancer adjuvant therapeutic approach using BSO to overcome the lack of efficient therapy in OCCC.</p>',
'date' => '2015-10-31',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26520442',
'doi' => '10.1007/s13277-015-4290-5',
'modified' => '2016-01-19 07:13:46',
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'name' => 'LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2',
'authors' => 'Maryem A. Hussein, Elina Shrestha, Mireille Ouimet, Tessa J. Barrett, Sarah Leone, Kathryn J. Moore, Yann Hérault, Edward A. Fisher, Michael J. Garabedian',
'description' => '<p>High cholesterol and diabetes are major risk factors for atherosclerosis. Regression of atherosclerosis is mediated in part by the Liver X Receptor (LXR) through the induction of genes involved in cholesterol transport and efflux. In the context of diabetes, regression of atherosclerosis is impaired. We proposed that changes in glucose levels modulate LXR-dependent gene expression. Using a mouse macrophage cell line (RAW 264.7) and primary bone marrow derived macrophages (BMDMs) cultured in normal or diabetes relevant high glucose conditions we found that high glucose inhibits the LXR-dependent expression of ATP-binding cassette transporter A1 (ABCA1), but not ABCG1. To probe for this mechanism, we surveyed the expression of a host of chromatin-modifying enzymes and found that Protein Arginine Methyltransferase 2 (PRMT2) was reduced in high compared to normal glucose conditions. Importantly, ABCA1 expression and ABCA1-mediated cholesterol efflux were reduced in Prmt2-/- compared to wild type BMDMs. Monocytes from diabetic mice also showed decreased expression of Prmt2 compared to non-diabetic counterparts. Thus, PRMT2 represents a glucose-sensitive factor that plays a role in LXR-mediated ABCA1-dependent cholesterol efflux and lends insight to the presence of increased atherosclerosis in diabetic patients.</p>',
'date' => '2015-08-19',
'pmid' => 'http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135218',
'doi' => '10.1371/journal.pone.0135218',
'modified' => '2015-11-10 13:43:13',
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'name' => 'Sensitive detection of chromatin-altering polymorphisms reveals autoimmune disease mechanisms.',
'authors' => 'Del Rosario RC, Poschmann J, Rouam SL, Png E, Khor CC, Hibberd ML, Prabhakar S',
'description' => 'Most disease associations detected by genome-wide association studies (GWAS) lie outside coding genes, but very few have been mapped to causal regulatory variants. Here, we present a method for detecting regulatory quantitative trait loci (QTLs) that does not require genotyping or whole-genome sequencing. The method combines deep, long-read chromatin immunoprecipitation-sequencing (ChIP-seq) with a statistical test that simultaneously scores peak height correlation and allelic imbalance: the genotype-independent signal correlation and imbalance (G-SCI) test. We performed histone acetylation ChIP-seq on 57 human lymphoblastoid cell lines and used the resulting reads to call 500,066 single-nucleotide polymorphisms de novo within regulatory elements. The G-SCI test annotated 8,764 of these as histone acetylation QTLs (haQTLs)-an order of magnitude larger than the set of candidates detected by expression QTL analysis. Lymphoblastoid haQTLs were highly predictive of autoimmune disease mechanisms. Thus, our method facilitates large-scale regulatory variant detection in any moderately sized cohort for which functional profiling data can be generated, thereby simplifying identification of causal variants within GWAS loci.',
'date' => '2015-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25799442',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
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'name' => 'Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus, Osmeridae), an anadromous Pacific smelt.',
'authors' => 'Candy JR, Campbell NR, Grinnell MH, Beacham TD, Larson WA, Narum SR',
'description' => 'Twelve eulachon (Thaleichthys pacificus, Osmeridae) populations ranging from Cook Inlet, Alaska and along the west coast of North America to the Columbia River were examined by restriction-site-associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high geneflow species. A total of 4104 single-nucleotide polymorphisms (SNPs) were discovered across the genome, with 193 putatively adaptive SNPs as determined by FST outlier tests. Estimates of population structure in eulachon with the putatively adaptive SNPs were similar, but provided greater resolution of stocks compared with a putatively neutral panel of 3911 SNPs or previous estimates with 14 microsatellites. A cline of increasing measures of genetic diversity from south to north was found in the adaptive panel, but not in the neutral markers (SNPs or microsatellites). This may indicate divergent selective pressures in differing freshwater and marine environments between regional eulachon populations and that these adaptive diversity patterns not seen with neutral markers could be a consideration when determining genetic boundaries for conservation purposes. Estimates of effective population size (Ne ) were similar with the neutral SNP panel and microsatellites and may be utilized to monitor population status for eulachon where census sizes are difficult to obtain. Greater differentiation with the panel of putatively adaptive SNPs provided higher individual assignment accuracy compared to the neutral panel or microsatellites for stock identification purposes. This study presents the first SNPs that have been developed for eulachon, and analyses with these markers highlighted the importance of integrating genome-wide neutral and adaptive genetic variation for the applications of conservation and management.',
'date' => '2015-03-03',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25737187',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
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'id' => '3076',
'name' => 'Silica nanoparticles induce oxidative stress, inflammation, and endothelial dysfunction in vitro via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling.',
'authors' => 'Caixia Guo,1,2 Yinye Xia,1,2 Piye Niu,1,2 Lizhen Jiang,1,2 Junchao Duan,1,2 Yang Yu,1,2 Xianqing Zhou,1,2 Yanbo Li,1,2 Zhiwei Sun1,2 ',
'description' => '<p><strong>Abstract:</strong><span> Despite the widespread application of silica nanoparticles (SiNPs) in industrial, commercial, and biomedical fields, their response to human cells has not been fully elucidated. Overall, little is known about the toxicological effects of SiNPs on the cardiovascular system. In this study, SiNPs with a 58 nm diameter were used to study their interaction with human umbilical vein endothelial cells (HUVECs). Dose- and time-dependent decrease in cell viability and damage on cell plasma-membrane integrity showed the cytotoxic potential of the SiNPs. SiNPs were found to induce oxidative stress, as evidenced by the significant elevation of reactive oxygen species generation and malondialdehyde production and downregulated activity in glutathione peroxidase. SiNPs also stimulated release of cytoprotective nitric oxide (NO) and upregulated inducible nitric oxide synthase (NOS) messenger ribonucleic acid, while downregulating endothelial NOS and ET-1 messenger ribonucleic acid, suggesting that SiNPs disturbed the NO/NOS system. SiNP-induced oxidative stress and NO/NOS imbalance resulted in endothelial dysfunction. SiNPs induced inflammation characterized by the upregulation of key inflammatory mediators, including IL-1β, IL-6, IL-8, TNFα, ICAM-1, VCAM-1, and MCP-1. In addition, SiNPs triggered the activation of the Nrf2-mediated antioxidant system, as evidenced by the induction of nuclear factor-κB and MAPK pathway activation. Our findings demonstrated that SiNPs could induce oxidative stress, inflammation, and NO/NOS system imbalance, and eventually lead to endothelial dysfunction via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling. This study indicated a potential deleterious effect of SiNPs on the vascular endothelium, which warrants more careful assessment of SiNPs before their application. </span></p>',
'date' => '2015-02-20',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25759575',
'doi' => '10.2147/IJN.S76114',
'modified' => '2016-11-29 10:33:26',
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'name' => 'Step-by-step preparation of proteins for mass spectrometric analysis.',
'authors' => 'Franz T, Li X.',
'description' => 'Nowadays, identification of proteins from biological samples by mass spectrometry is widely used. In principle there are two scenarios. Proteins are pre-fractionated in some way, e.g. by gel electrophoresis or are analyzed as complex mixture (shot gun). Shot gun proteomics became recently more popular, because of technological developments on the mass spectrometer side which allows now the identification of several thousand proteins from complex biological matrix. However, in many cases it is still useful to separate proteins first in a gel. But not only mass spectrometer technology made progress. This is also true for the sample preparation. Recently, protocols and techniques were developed which make the analysis of starting material in the low microgram range possible and also simplify the whole procedure. Detailed protocols will be described allowing also inexperienced beginners to get good results. ',
'date' => '0000-00-00',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/25820726',
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'name' => 'Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours',
'authors' => 'Bujko M, Kober P, Statkiewicz M, Mikula M, Ligaj M, Zwierzchowski L, Ostrowski J, Siedlecki JA',
'description' => 'Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). Results indicate that μ-protocadherin downregulation occurs during early stages of tumourigenesis and progression into the adenoma-carcinoma sequence. Epigenetic mechanisms are involved in this silencing.',
'date' => '0000-00-00',
'pmid' => 'http://www.hindawi.com/journals/grp/2015/317093/',
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'description' => '<p>We use the <strong>Bioruptor</strong> sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The <strong>H3K4me3 antibody</strong> is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.</p>
<p>Diagenode provides not only very good products for research but also an excellent customer support.</p>',
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'author' => 'Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland',
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<p>Diagenode provides not only very good products for research but also an excellent customer support.</p><cite>Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany</cite></blockquote>
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$featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>To expedite the larger-scale phenotyping of pollen number difference within <em>Arabidopsis</em> family, we have modified an existing method more suitable for our purpose of pollen counting using a Bioruptor<sup>®</sup>. We grew four plants per genotype. Three flower buds per plant were harvested and dried at 65°C overnight. We collected flower buds with mature pollen, but before the anthers were opened (flower stage 13). We did not use the first and second flowers of the inflorescence, as they tend to show developmentally immature flower morphologies. 30 <span>μ</span>l of 5% Tween-20 is added to the dried flower and samples are sonicated using the Bioruptor<sup>®</sup> Plus with high power mode for 10 cycles (<span>sonication cycle: 30 sec ON, 30 sec OFF) </span>to separate pollen grains from the anthers. Then, all pollen solutions are measured with a cell counter.</p><cite>Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland</cite></blockquote>
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</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack" data-reveal-id="cartModal-1794" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml tube holder for Bioruptor® Standard &...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack"><img src="/img/product/shearing_technologies/B01200014_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200014</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1795" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1795" id="CartAdd/1795Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1795" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'B01200014',
'1850',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'B01200014',
'1850',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack" data-reveal-id="cartModal-1795" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">50 ml tube holder for Bioruptor® Standard &...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/water-cooler-115v-1-unit"><img src="/img/product/shearing_technologies/B02010003%20_water_cooler.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02010003</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1814" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1814" id="CartAdd/1814Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1814" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Water cooler, 115V</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 115V',
'B02010003',
'4950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 115V',
'B02010003',
'4950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="water-cooler-115v-1-unit" data-reveal-id="cartModal-1814" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Water cooler, 115V</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/water-cooler-230v-1-unit"><img src="/img/product/shearing_technologies/B02010003%20_water_cooler.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02010002</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1813" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1813" id="CartAdd/1813Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1813" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Water cooler, 230V</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 230V',
'B02010002',
'4950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 230V',
'B02010002',
'4950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="water-cooler-230v-1-unit" data-reveal-id="cartModal-1813" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Water cooler, 230V</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/single-cycle-valve-for-water-cooler-1-unit"><img src="/img/product/shearing_technologies/B02020004%20_single_cycle_valve.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02020005</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1816" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1816" id="CartAdd/1816Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1816" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Single Cycle Valve for Water Cooler</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="single-cycle-valve-for-water-cooler-1-unit" data-reveal-id="cartModal-1816" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Single Cycle Valve for Water Cooler</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/metallic-soundproof-box-1-unit"><img src="/img/product/shearing_technologies/B01200001%20_soundproof_box.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200001</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1790" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1790" id="CartAdd/1790Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1790" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Metallic soundproof box</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="metallic-soundproof-box-1-unit" data-reveal-id="cartModal-1790" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Metallic soundproof box</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-ml-bioruptor-microtubes-500-tubes"><img src="/img/product/shearing_technologies/C30010013_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010013</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2627" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2627" id="CartAdd/2627Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2627" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5 ml Bioruptor<sup>®</sup> Plus Microtubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-ml-bioruptor-microtubes-500-tubes" data-reveal-id="cartModal-2627" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5 ml Bioruptor® Plus Microtubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010009</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Plus TPX tubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/rna-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000010</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2616" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2616" id="CartAdd/2616Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2616" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> RNA Extraction kit for Bioruptor Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
'165',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
'165',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="rna-extraction-kit-50-rxns" data-reveal-id="cartModal-2616" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">RNA Extraction kit for Bioruptor Plus</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/protein-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000020</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2617" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<h6 style="height:60px">Bioruptor® Plus DNA QC kit</h6>
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'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>',
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'date' => '0000-00-00',
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<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
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</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
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<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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</p>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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'type' => 'ACC',
'search_order' => '01-Accessory',
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'slug' => '15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack',
'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus',
'meta_keywords' => '',
'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus',
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'name' => '50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'description' => '<p><span>The attachment for 50 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 3 x 50 ml tubes at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
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'sf_code' => 'B01200014-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1850',
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'price_GBP' => '1600',
'price_JPY' => '289800',
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'price_AUD' => '4625',
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'slug' => '50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack',
'meta_title' => '50 ml tube holder for Bioruptor Standard & Bioruptor Plus',
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'meta_description' => '50 ml tube holder for Bioruptor Standard & Bioruptor Plus',
'modified' => '2017-08-19 10:37:17',
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'name' => 'Water cooler, 115V',
'description' => '<p><span>This water cooling system allows continuous (Bioruptor® Standard or Plus) or regulated cooling (Bioruptor® Plus or Pico) of the Bioruptor sonication bath. The regulated valve (</span><a href="http://www.diagenode.com/en/catalog/sonication-54/bioruptor-accessories-56/water-cooler-accessories-60/product/single-cycle-valve-bioruptor-pico-bioruptor-plus--369" target="_blank">Single Cycle valve</a><span>) ensures that water will only be replaced during the OFF cycle to avoid any interference between the water flow and the sonication process.</span></p>',
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<tr>
<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
</tr>
<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
</tr>
<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
</tr>
<tr>
<td>Safety classification</td>
<td>Class I/NFL</td>
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<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
</tr>
<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
</tr>
<tr>
<td>Refrigerant</td>
<td>R290</td>
</tr>
<tr>
<td>Refrigerant quantity</td>
<td>0.04 kg</td>
</tr>
<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
</tr>
<tr>
<td>max. delivery</td>
<td>20 l/min</td>
</tr>
<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
</tr>
<tr>
<td>max. delivery (suction)</td>
<td>17 l/min</td>
</tr>
<tr>
<td>Pump connection</td>
<td>G 1/4" female thread</td>
</tr>
<tr>
<td>min. filling capacity</td>
<td>1.4 l</td>
</tr>
<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
</tr>
<tr>
<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
</tr>
<tr>
<td>Net weight</td>
<td>23 kg</td>
</tr>
<tr>
<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
</tr>
<tr>
<td>max. current</td>
<td>2.8 A</td>
</tr>
<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
</tr>
<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
</tr>
<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
</tr>
</tbody>
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'format' => '1 unit',
'catalog_number' => 'B02010003',
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'sf_code' => 'B02010003-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '4850',
'price_USD' => '4950',
'price_GBP' => '4300',
'price_JPY' => '759750',
'price_CNY' => '',
'price_AUD' => '12375',
'country' => 'ALL',
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'slug' => 'water-cooler-115v-1-unit',
'meta_title' => 'Water cooler, 115V',
'meta_keywords' => '',
'meta_description' => 'Water cooler, 115V',
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'name' => 'Water cooler, 230V',
'description' => '<p><span>This water cooling system allows continuous (Bioruptor® Standard or Plus) or regulated cooling (Bioruptor® Plus or Pico) of the Bioruptor sonication bath. The regulated valve (</span><a href="http://www.diagenode.com/en/catalog/sonication-54/bioruptor-accessories-56/water-cooler-accessories-60/product/single-cycle-valve-bioruptor-pico-bioruptor-plus--369" target="_blank">Single Cycle valve</a><span>) ensures that water will only be replaced during the OFF cycle to avoid any interference between the water flow and the sonication process.</span></p>',
'label1' => 'Characteristics',
'info1' => '<table>
<tbody>
<tr>
<td>Operating temperature range</td>
<td>-20 ... 40°C</td>
</tr>
<tr>
<td>Temperature adjustment</td>
<td>digital</td>
</tr>
<tr>
<td>Temperature indication</td>
<td>digital</td>
</tr>
<tr>
<td>Internal temperature sensor</td>
<td>Pt100</td>
</tr>
<tr>
<td>Resolution of display</td>
<td>0.1 K</td>
</tr>
<tr>
<td>Temperature stability at -10°C</td>
<td>0,2 K</td>
</tr>
<tr>
<td>Safety classification</td>
<td>Class I/NFL</td>
</tr>
<tr>
<td>Cooling power<br /> at 15°C<br /> at 0°C<br /> at -10°C<br /> at -20°C</td>
<td>0.3 kW<br /> 0.2 kW<br /> 0.14 kW<br /> 0.07 kW</td>
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<tr>
<td>Refrigeration machine</td>
<td>air-cooled, natural<br /> refrigerant</td>
</tr>
<tr>
<td>Refrigerant</td>
<td>R290</td>
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<tr>
<td>Refrigerant quantity</td>
<td>0.04 kg</td>
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<tr>
<td>Circulation pump</td>
<td>Pressure- and suction pump</td>
</tr>
<tr>
<td>max. delivery</td>
<td>20 l/min</td>
</tr>
<tr>
<td>max. delivery pressure</td>
<td>0.2 bar</td>
</tr>
<tr>
<td>max. delivery (suction)</td>
<td>17 l/min</td>
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<tr>
<td>Pump connection</td>
<td>G 1/4" female thread</td>
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<tr>
<td>min. filling capacity</td>
<td>1.4 l</td>
</tr>
<tr>
<td>Volume of expansion</td>
<td>2.6 l</td>
</tr>
<tr>
<td>Overall dimensions WxDxH **</td>
<td>225x360x380 mm</td>
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<tr>
<td>Net weight</td>
<td>23 kg</td>
</tr>
<tr>
<td>Power supply requirement</td>
<td>230V 1~ 50/60Hz</td>
</tr>
<tr>
<td>max. current</td>
<td>2.8 A</td>
</tr>
<tr>
<td>Fuse (1 phase)</td>
<td>4 A</td>
</tr>
<tr>
<td>min. ambient temperature</td>
<td>5°C</td>
</tr>
<tr>
<td>max. ambient temperature</td>
<td>40°C</td>
</tr>
</tbody>
</table>',
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'catalog_number' => 'B02010002',
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'sf_code' => 'B02010002-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '4850',
'price_USD' => '4950',
'price_GBP' => '4300',
'price_JPY' => '759750',
'price_CNY' => '',
'price_AUD' => '12375',
'country' => 'ALL',
'except_countries' => 'None',
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'featured' => false,
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'last_datasheet_update' => '0000-00-00',
'slug' => 'water-cooler-230v-1-unit',
'meta_title' => 'Water cooler, 230V',
'meta_keywords' => '',
'meta_description' => 'Water cooler, 230V',
'modified' => '2018-02-14 16:26:52',
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(int) 5 => array(
'id' => '1816',
'antibody_id' => null,
'name' => 'Single Cycle Valve for Water Cooler',
'description' => '<p><span>The Single Cycle Valve for the Bioruptor® controls the water flow between the Bioruptor® Pico (B01060001) / Bioruptor® Plus (UCD-300) and the water cooler. Cold water cycles into the Bioruptor® sonication bath only during the off cycle (no sonication) and is stopped during the on cycle (sonication). This reduces any water flow disturbance in the tank and allows for more efficient sonication of samples.</span></p>
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'label1' => '特徴',
'info1' => '<ul>
<li>Compatible with Bioruptor® Pico and Bioruptor® Plus</li>
<li>Increases shearing efficiency</li>
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'label2' => '',
'info2' => '<p></p>
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'info3' => '<p></p>
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'format' => '1 unit',
'catalog_number' => 'B02020005',
'old_catalog_number' => '',
'sf_code' => 'B02020005-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1050',
'price_USD' => '950',
'price_GBP' => '900',
'price_JPY' => '164480',
'price_CNY' => '',
'price_AUD' => '2375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'single-cycle-valve-for-water-cooler-1-unit',
'meta_title' => 'Single Cycle Valve for Water Cooler',
'meta_keywords' => '',
'meta_description' => 'Single Cycle Valve for Water Cooler',
'modified' => '2021-03-23 04:10:10',
'created' => '2015-06-29 14:08:20',
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(int) 6 => array(
'id' => '1790',
'antibody_id' => null,
'name' => 'Metallic soundproof box',
'description' => '<p><span>This metallic soundproof box has been designed for a highly efficient acoustic insulation of the Bioruptor® sonication bath. This box is only compatible with Bioruptor® standard and Bioruptor® Plus. Can be stored at room temperature or in a cold room (4°C).</span></p>',
'label1' => 'Characteristics',
'info1' => '<ul>
<li>Compatible with Bioruptor® standard and Bioruptor® Plus</li>
<li>Very efficient acoustic insulation of the Bioruptor waterbath.</li>
<li>Dimensions: 35(W) x 30(D) x 52(H) mm</li>
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<p><strong>Dimensions :</strong><br />Width : 35 cm<br />Depth : 35 cm<br />Height : 52 cm</p>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'B01200001',
'old_catalog_number' => 'BioBox-200',
'sf_code' => 'B01200001-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1550',
'price_USD' => '1450',
'price_GBP' => '1400',
'price_JPY' => '242810',
'price_CNY' => '',
'price_AUD' => '3625',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
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'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'metallic-soundproof-box-1-unit',
'meta_title' => 'Metallic soundproof box',
'meta_keywords' => '',
'meta_description' => 'Metallic soundproof box',
'modified' => '2019-10-20 13:42:38',
'created' => '2015-06-29 14:08:20',
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'id' => '2627',
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'name' => '0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'description' => '<p><span>The 0.5 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Standard (B01010001 or Bioruptor® Plus (B01020001) and are strongly recommended for DNA and chromatin shearing applications using this instruments. Recommended sample volume: 50 μl - 100 μl.</span></p>',
'label1' => '',
'info1' => '',
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'label3' => '',
'info3' => '',
'format' => '500 tubes',
'catalog_number' => 'C30010013',
'old_catalog_number' => 'WA-004-0500',
'sf_code' => 'C30010013-1862',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '105',
'price_USD' => '145',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '362',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
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'online' => true,
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'last_datasheet_update' => '0000-00-00',
'slug' => '0-5-ml-bioruptor-microtubes-500-tubes',
'meta_title' => '0.5 ml Bioruptor® Microtubes',
'meta_keywords' => '',
'meta_description' => '0.5 ml Bioruptor® Microtubes',
'modified' => '2021-12-20 12:30:33',
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'name' => '0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'description' => '<p><span>The attachment for 0.5 ml (Standard/Plus) tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 12 samples at a time.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
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'format' => '1 pack',
'catalog_number' => 'B01200043',
'old_catalog_number' => 'UCD-pack 0.5',
'sf_code' => 'B01200043-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '1300',
'price_USD' => '1800',
'price_GBP' => '1150',
'price_JPY' => '203645',
'price_CNY' => '',
'price_AUD' => '4500',
'country' => 'ALL',
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'slug' => '0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '0.5/0.65 ml tube holder for Bioruptor® Standard & Plus & Pico',
'modified' => '2020-03-09 09:53:05',
'created' => '2015-06-29 14:08:20',
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'name' => '15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'description' => '<p><span>TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultrasound to the sample. TPX® tubes have been validated for use on the Bioruptors® Standard and Bioruptors® Plus. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
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'label3' => '',
'info3' => '',
'format' => '50 pc',
'catalog_number' => 'C30010009',
'old_catalog_number' => 'M-UN-15',
'sf_code' => 'C30010009-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '100',
'price_USD' => '120',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
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'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-tpx-tubes-50-pc',
'meta_title' => '15 ml TPX tubes',
'meta_keywords' => '',
'meta_description' => '15 ml TPX tubes',
'modified' => '2017-08-19 18:39:10',
'created' => '2015-06-29 14:08:20',
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'name' => 'RNA Extraction kit for Bioruptor Plus',
'description' => '<p><span>The combination of the RNA extraction kit and the </span>Bioruptor<sup>®</sup><span> Plus maintains the integrity of RNA and ensures maximum yield of RNA. Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disruption releases RNA, while homogenization reduces sample viscosity to facilitate RNA purification. </span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C20000010',
'old_catalog_number' => 'AL-001-0050',
'sf_code' => 'C20000010-',
'type' => 'REF',
'search_order' => '01-Accessory',
'price_EUR' => '145',
'price_USD' => '165',
'price_GBP' => '145',
'price_JPY' => '22715',
'price_CNY' => '',
'price_AUD' => '412',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
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'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'rna-extraction-kit-50-rxns',
'meta_title' => 'RNA Extraction kit for Bioruptor Plus | Diagenode',
'meta_keywords' => 'RNA Extraction,RNA Protocol',
'meta_description' => 'Isolation of intact RNA is essential for many techniques used in gene expression analysis. RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit',
'modified' => '2018-11-26 11:46:46',
'created' => '2015-06-29 14:08:20',
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'name' => 'Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
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<ul>
<li>Fast and simple</li>
<li>No contamination between samples</li>
<li>Efficient</li>
<li>Gentle processing</li>
<li>Reproducible</li>
<li>Temperature controlled</li>
<li>Multiplexing capability</li>
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'name' => 'Bioruptor<sup>®</sup> Plus DNA QC kit',
'description' => '<p>We have developed the DNA Quality Control Kit for you to be able to track the efficiency of your <strong>Bioruptor</strong><span><strong>® Plus/Standard</strong> </span><span>and to figure out the right time for servicing. After the sonication, if your QC does not reach our standards, please, contact Diagenode for recommendations. However, if your QC pass, we wish you every success with your Bioruptor</span><span>®</span><span>.</span></p>',
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<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. <strong>Diagenode's Bioruptor</strong><sup>® </sup><strong>Plus</strong> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.</div>
<div class="large-12 columns"></div>
<div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/pro-rna-extract-fig3a.png" /></div>
<div class="small-6 medium-6 large-6 columns text-center end small-offset-3"><small><strong>Efficient extraction of pure RNA with high RIN. </strong><br /><span>Total RNA profiles from mouse brain. Tissue was disrupted with Bioruptor<sup>®</sup> Plus as described in the protocol and analyzed on BioAnalyzer (Agilent). Note that small RNAs are present in all profiles indicating that the RNA is largely intact.</span></small></div>
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div>
</div>
<p></p>
<div class="row">
<div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" />
<p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p>
</div>
<div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" />
<p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p>
</div>
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<div class="large-12 columns">Diagenode's high yields FFPE DNA extraction using Bioruptor<sup><span>®</span></sup> is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by traditional methods or can be sheared with the Bioruptor<sup>®</sup> Pico ultrasonicator for downstream NGS library prep using the MicroPlex Library Preparation Kit.</div>
<div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/ffpe_workflow.png" /></div>
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<div class="small-12 medium-12 large-12 columns"><br />
<p><span>Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</span></p>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/TAB-BR-comparaison.pdf" target="_blank"><img src="https://www.diagenode.com/img/bouton-comparaison.png" /></a></p>
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<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">Technology explained</h2>
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<h2 style="text-align: center;">Reproductibility is our priority</h2>
</div>
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<div><img src="https://www.diagenode.com/img/shearing/reproductibility.png" alt="reproductibility" />
<p class="bottom_note"></p>
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<h2 style="text-align: center;">An affordable instrument for wide range of applications</h2>
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<p style="text-align: center;">Designed for any researchers, the Bioruptor gives the user the right level of flexibility.</p>
<table style="width: 972px;">
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<th class="text-center" style="width: 126px; height: 56px;">Bioruptor</th>
<th class="text-center" style="width: 141px; height: 56px;">Cup Horn Sonicators</th>
<th class="text-center" style="width: 156px; height: 56px;">Focused <br />ultra-sonicators</th>
<th class="text-center" style="width: 155px; height: 56px;">Multi Sample Sonicator</th>
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<td style="width: 380px; height: 38px;">Instrument pricing</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
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<td style="width: 380px; height: 38px;">Consumables pricing</td>
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<td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td>
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<td style="width: 380px; height: 38px;">Range of applications</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
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<td style="width: 380px; height: 38px;">Scalable and sample volume flexibility</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i></center></td>
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<td style="width: 380px; height: 38px;">Throughput</td>
<td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
<td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td>
</tr>
</tbody>
</table>
<div class="content_block">
<div class="centered row">
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">Bioruptor ultrasonication for best results in:</h2>
<p><b><span>✓ Chromatin shearing</span><span> </span><span style="font-weight: 400;">- Industry leader in accurate and tight fragment ranges</span></b></p>
<p><b><span>✓ DNA shearing</span><span> </span><span style="font-weight: 400;">- Excellent results for optimal fragment lengths in NGS library prep</span></b></p>
<p><b><span>✓<span> </span></span>Protein aggregation studies </b><span style="font-weight: 400;">- Standardizing seeding with the robust Bioruptor.<br /></span><i><span style="font-weight: 400;">Read the app note by Dr. Kelvin Luk at the University of Pennsylvania </span></i><a href="https://www.diagenode.com/en/documents/standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease" style="color: #13b29c;"><i><span style="font-weight: 400;">“Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>3D genome analysis with Hi-C</b><span style="font-weight: 400;"> - Preparing chromatin libraries with high-quality sonication.<br /></span><i><span style="font-weight: 400;">Read the app note, “</span></i><a href="https://www.diagenode.com/en/documents/applicationnote-arima-low-input" style="color: #13b29c;"><i><span style="font-weight: 400;">Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit”</span></i></a></p>
<p><b><b><span>✓<span> </span></span></b>Mass spectrometry</b> <b>and increasing protein identification</b><span style="font-weight: 400;">- Sample preparation using Preomics iST and Bioruptor sonication.<br /></span><i><span style="font-weight: 400;">Read the app note “</span></i><a href="https://www.diagenode.com/en/documents/wp-ist-adaptators" style="color: #13b29c;"><i><span style="font-weight: 400;">Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder”</span></i></a></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>Cell lysis, liposome prep, protein extraction, RNA extraction and more</b></span></p>
<p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>CUT&RUN –Sonication of input DNA (for enrichment comparison) for NGS</b></span></p>
</div>
</div>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
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<p><span>Disruption of bacterial cells is required for releasing biological molecules from within the cells for various biological applications. Ultrasound technology has been widely used for such cell lysis applications. Ultrasonic disruption is based on cavitation, the creation of cavities in cell suspension. These cavities or small bubbles of dissolved gases or vapors arise from the alteration of pressure in liquids. The bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming leading to mechanical stress that effectively lyses cells. Diagenode’s Bioruptor</span><span><sup>®</sup> </span><span>sonicaton device uses state-of-the-art ultrasound technology to disrupt cells for biological, chemical, pharmaceutical, and industrial applications. </span></p>
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'description' => '<p><span>Just follow these four steps for shearing chromatin, and you’ll be well on your way to excellent ChIP results without the troubleshooting headache. </span></p>',
'image_id' => null,
'type' => 'Protocol',
'url' => 'files/protocols/WP-4steps-fragmentation-for-chip-V1_09_14.pdf',
'slug' => 'wp-4steps-fragmentation-for-chip-v1-09-14',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-01-14 14:32:45',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '18',
'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus',
'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>',
'image_id' => '214',
'type' => 'Protocol',
'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf',
'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:41:17',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '27',
'name' => 'Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus',
'description' => '<p>Standard procedure to shear Chromatin from Yeast. </p>',
'image_id' => '218',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_Shearing_in_Yeast_Cells_with_Bioruptor_protocol.pdf',
'slug' => 'chromatin-shearing-in-yeast-cells-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-17 19:32:52',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '33',
'name' => 'DNA Shearing for Bioruptor® Standard and Plus',
'description' => '<p><span>For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor</span><sup><span>® </span></sup><span>0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-0500). </span></p>',
'image_id' => '201',
'type' => 'Protocol',
'url' => 'files/protocols/DNA_Shearing_for_Standard_and_Plus_protocol.pdf',
'slug' => 'dna-shearing-for-standard-and-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:01',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '10',
'name' => 'FFPE DNA extraction protocol using the Bioruptor®',
'description' => '<p><span>The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, providing an invaluable repository of information for genetic analysis. These samples can be analyzed for a wealth of applications including biomarker discovery, drug development, and cancer research. </span><span>Diagenode’s FFPE DNA Extraction kit is optimized for the extraction of DNA from FFPE tissue sections in conjunction with the Bioruptor</span><span>®</span><span>. </span></p>',
'image_id' => '204',
'type' => 'Protocol',
'url' => 'files/protocols/FFPE_DNA_extraction_protocol.pdf',
'slug' => 'ffpe-dna-extraction-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:42:46',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 8 => array(
'id' => '61',
'name' => 'Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis',
'description' => '<p>Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck Institute for Biochemistry, Martinsried, Germany are using the Bioruptor<span>® </span>for their research using C. elegans, a powerful metazoan model system to study and understand fundamental problems in biology. Traditionally C. elegans research has focused mainly on genetics. However, recent studies suggest that C. elegans can also be a facile system for biochemistry. Tagged proteins can be expressed in vivo and interacting partners can be identified using various biochemical approaches. In addition, protein networks and pathways can be identified by combining genetic screens with biochemistry.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/protocols/mass_spectrometry -Celegans-protocol.pdf',
'slug' => 'mass_spectrometry -Celegans-protocol',
'meta_keywords' => 'mass_spectrometry -Celegans-protocol',
'meta_description' => 'mass_spectrometry -Celegans-protocol',
'modified' => '2016-01-15 12:07:23',
'created' => '2016-01-15 11:55:19',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 9 => array(
'id' => '62',
'name' => 'Mass-Spectrometry: procedure for shotgun proteomics',
'description' => '<p>Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in addition to degradation and removal of unwanted components like nucleic acids. Therefore, it requires mechanical breakdown accompanied by denaturing agents and heating of the samples. Matthias Mann and his team from the Department of Proteomics and Signal Transduction at the Max Plank Institute of Biochemistry developed and employ highly efficient protocols for protein isolation in conjunction with Bioruptor to perform quantitative proteomics.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/protocols/bioruptor-mass_spectometry-protocol.pdf',
'slug' => 'bioruptor-mass_spectometry-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-02-05 14:22:56',
'created' => '2016-02-05 14:22:56',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 10 => array(
'id' => '23',
'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus',
'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>',
'image_id' => '213',
'type' => 'Protocol',
'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf',
'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:40:14',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 11 => array(
'id' => '26',
'name' => 'RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit',
'description' => '<p><span>Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disruption releases RNA, while homogenization reduces sample viscosity to facilitate RNA purification. Diagenode’s Bioruptor</span><span>® </span><span>Sonicator uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues in one step. Diagenode’s RNA extraction reagent (included in the RNA extraction kit) is used as sonication medium and maintains the integrity of RNA while disrupting cells and dissolving cell components. </span></p>',
'image_id' => '216',
'type' => 'Protocol',
'url' => 'files/protocols/RNA_Extraction_from_Tissue_with_Bioruptor_Standard_Plus_protocol.pdf',
'slug' => 'rna-extraction-from-tissue-with-bioruptor-standard-plus-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:45:55',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 12 => array(
'id' => '43',
'name' => 'TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®',
'description' => '<p><span>The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a native environment. Molecular complexes can then be isolated and used for various applications for the identification of partners. Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes combined the TAP tagging with the Bioruptor</span><span>®</span><span>. This combination is essential for the efficient purification of a chromatin protein. </span></p>',
'image_id' => null,
'type' => 'Protocol',
'url' => 'files/protocols/TAP-TAG-procedure-for-bioruptor.pdf',
'slug' => 'tap-tag-procedure-for-bioruptor',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:40',
'created' => '2015-08-10 10:50:46',
'ProductsProtocol' => array(
[maximum depth reached]
)
),
(int) 13 => array(
'id' => '24',
'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®',
'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>',
'image_id' => '220',
'type' => 'Protocol',
'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf',
'slug' => 'western-blot-with-bioruptor-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:39:29',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4454',
'name' => 'Histone lysine demethylase inhibition reprograms prostate cancermetabolism and mechanics.',
'authors' => 'Chianese Ugo and Papulino Chiara and Passaro Eugenia andEvers Tom Mj and Babaei Mehrad and Toraldo Antonella andDe Marchi Tommaso and Niméus Emma and Carafa Vincenzo andNicoletti Maria Maddalena and Del Gaudio Nunzio andIaccarino Nunzia an',
'description' => '<p>OBJECTIVE: Aberrant activity of androgen receptor (AR) is the primary cause underlying development and progression of prostate cancer (PCa) and castration-resistant PCa (CRPC). Androgen signaling regulates gene transcription and lipid metabolism, facilitating tumor growth and therapy resistance in early and advanced PCa. Although direct AR signaling inhibitors exist, AR expression and function can also be epigenetically regulated. Specifically, lysine (K)-specific demethylases (KDMs), which are often overexpressed in PCa and CRPC phenotypes, regulate the AR transcriptional program. METHODS: We investigated LSD1/UTX inhibition, two KDMs, in PCa and CRPC using a multi-omics approach. We first performed a mitochondrial stress test to evaluate respiratory capacity after treatment with MC3324, a dual KDM-inhibitor, and then carried out lipidomic, proteomic, and metabolic analyses. We also investigated mechanical cellular properties with acoustic force spectroscopy. RESULTS: MC3324 induced a global increase in H3K4me2 and H3K27me3 accompanied by significant growth arrest and apoptosis in androgen-responsive and -unresponsive PCa systems. LSD1/UTX inhibition downregulated AR at both transcriptional and non-transcriptional level, showing cancer selectivity, indicating its potential use in resistance to androgen deprivation therapy. Since MC3324 impaired metabolic activity, by modifying the protein and lipid content in PCa and CRPC cell lines. Epigenetic inhibition of LSD1/UTX disrupted mitochondrial ATP production and mediated lipid plasticity, which affected the phosphocholine class, an important structural element for the cell membrane in PCa and CRPC associated with changes in physical and mechanical properties of cancer cells. CONCLUSIONS: Our data suggest a network in which epigenetics, hormone signaling, metabolite availability, lipid content, and mechano-metabolic process are closely related. This network may be able to identify additional hotspots for pharmacological intervention and underscores the key role of KDM-mediated epigenetic modulation in PCa and CRPC.</p>',
'date' => '2022-08-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35944897',
'doi' => '10.1016/j.molmet.2022.101561',
'modified' => '2022-10-21 09:37:56',
'created' => '2022-09-28 09:53:13',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4214',
'name' => 'Comprehensive characterization of the epigenetic landscape in Multiple Myeloma',
'authors' => 'Elina Alaterre et al.',
'description' => '<p>Background: Human multiple myeloma (MM) cell lines (HMCLs) have been widely used to understand the<br />molecular processes that drive MM biology. Epigenetic modifications are involved in MM development,<br />progression, and drug resistance. A comprehensive characterization of the epigenetic landscape of MM would<br />advance our understanding of MM pathophysiology and may attempt to identify new therapeutic targets.<br />Methods: We performed chromatin immunoprecipitation sequencing to analyze histone mark changes<br />(H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3 and H3K36me3) on 16 HMCLs.<br />Results: Differential analysis of histone modification profiles highlighted links between histone modifications<br />and cytogenetic abnormalities or recurrent mutations. Using histone modifications associated to enhancer<br />regions, we identified super-enhancers (SE) associated with genes involved in MM biology. We also identified<br />promoters of genes enriched in H3K9me3 and H3K27me3 repressive marks associated to potential tumor<br />suppressor functions. The prognostic value of genes associated with repressive domains and SE was used to<br />build two distinct scores identifying high-risk MM patients in two independent cohorts (CoMMpass cohort; n =<br />674 and Montpellier cohort; n = 69). Finally, we explored H3K4me3 marks comparing drug-resistant and<br />-sensitive HMCLs to identify regions involved in drug resistance. From these data, we developed epigenetic<br />biomarkers based on the H3K4me3 modification predicting MM cell response to lenalidomide and histone<br />deacetylase inhibitors (HDACi).<br />Conclusions: The epigenetic landscape of MM cells represents a unique resource for future biological studies.<br />Furthermore, risk-scores based on SE and repressive regions together with epigenetic biomarkers of drug<br />response could represent new tools for precision medicine in MM.</p>',
'date' => '2022-01-16',
'pmid' => 'https://www.thno.org/v12p1715',
'doi' => '10.7150/thno.54453',
'modified' => '2022-01-27 13:17:28',
'created' => '2022-01-27 13:14:17',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4164',
'name' => 'Chromatin dysregulation associated with NSD1 mutation in head and necksquamous cell carcinoma.',
'authors' => 'Farhangdoost, Nargess et al. ',
'description' => '<p>Chromatin dysregulation has emerged as an important mechanism of oncogenesis. To develop targeted treatments, it is important to understand the transcriptomic consequences of mutations in chromatin modifier genes. Recently, mutations in the histone methyltransferase gene nuclear receptor binding SET domain protein 1 (NSD1) have been identified in a subset of common and deadly head and neck squamous cell carcinomas (HNSCCs). Here, we use genome-wide approaches and genome editing to dissect the downstream effects of loss of NSD1 in HNSCC. We demonstrate that NSD1 mutations are responsible for loss of intergenic H3K36me2 domains, followed by loss of DNA methylation and gain of H3K27me3 in the affected genomic regions. In addition, those regions are enriched in cis-regulatory elements, and subsequent loss of H3K27ac correlates with reduced expression of their target genes. Our analysis identifies genes and pathways affected by the loss of NSD1 and paves the way to further understanding the interplay among chromatin modifications in cancer.</p>',
'date' => '2021-02-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33626351',
'doi' => '10.1016/j.celrep.2021.108769',
'modified' => '2021-12-21 15:35:45',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3396',
'name' => 'The Itaconate Pathway Is a Central Regulatory Node Linking Innate Immune Tolerance and Trained Immunity',
'authors' => 'Domínguez-Andrés Jorge, Novakovic Boris, Li Yang, Scicluna Brendon P., Gresnigt Mark S., Arts Rob J.W., Oosting Marije, Moorlag Simone J.C.F.M., Groh Laszlo A., Zwaag Jelle, Koch Rebecca M., ter Horst Rob, Joosten Leo A.B., Wijmenga Cisca, Michelucci Ales',
'description' => '<p>Sepsis involves simultaneous hyperactivation of the immune system and immune paralysis, leading to both organ dysfunction and increased susceptibility to secondary infections. Acute activation of myeloid cells induced itaconate synthesis, which subsequently mediated innate immune tolerance in human monocytes. In contrast, induction of trained immunity by b-glucan counteracted tolerance induced in a model of human endotoxemia by inhibiting the expression of immune-responsive gene 1 (IRG1), the enzyme that controls itaconate synthesis. b-Glucan also increased the expression of succinate dehydrogenase (SDH), contributing to the integrity of the TCA cycle and leading to an enhanced innate immune response after secondary stimulation. The role of itaconate was further validated by IRG1 and SDH polymorphisms that modulate induction of tolerance and trained immunity in human monocytes. These data demonstrate the importance of the IRG1-itaconateSDH axis in the development of immune tolerance and training and highlight the potential of b-glucaninduced trained immunity to revert immunoparalysis.</p>',
'date' => '2018-10-01',
'pmid' => 'http://www.pubmed.gov/30293776',
'doi' => '10.1016/j.cmet.2018.09.003',
'modified' => '2018-11-22 15:18:30',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3377',
'name' => 'Interplay of cell–cell contacts and RhoA/MRTF‐A signaling regulates cardiomyocyte identity',
'authors' => 'Dorn et al',
'description' => '<p><span>Cell–cell and cell–matrix interactions guide organ development and homeostasis by controlling lineage specification and maintenance, but the underlying molecular principles are largely unknown. Here, we show that in human developing cardiomyocytes cell–cell contacts at the intercalated disk connect to remodeling of the actin cytoskeleton by regulating the RhoA‐</span><span class="styled-content fixed-case">ROCK</span><span><span> </span>signaling to maintain an active<span> </span></span><span class="styled-content fixed-case">MRTF</span><span>/</span><span class="styled-content fixed-case">SRF</span><span><span> </span>transcriptional program essential for cardiomyocyte identity. Genetic perturbation of this mechanosensory pathway activates an ectopic fat gene program during cardiomyocyte differentiation, which ultimately primes the cells to switch to the brown/beige adipocyte lineage in response to adipogenesis‐inducing signals. We also demonstrate by<span> </span></span><em>in vivo</em><span><span> </span>fate mapping and clonal analysis of cardiac progenitors that cardiac fat and a subset of cardiac muscle arise from a common precursor expressing Isl1 and Wt1 during heart development, suggesting related mechanisms of determination between the two lineages.</span></p>',
'date' => '2018-05-15',
'pmid' => 'http://emboj.embopress.org/content/early/2018/05/15/embj.201798133',
'doi' => '10.15252/embj.201798133',
'modified' => '2018-05-22 22:50:53',
'created' => '2018-05-22 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3368',
'name' => 'Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity',
'authors' => 'Darya A. Haas, Arno Meiler, Katharina Geiger, Carola Vogt, Ellen Preuss, Georg Kochs, Andreas Pichlmair',
'description' => '<p><span>Viruses have evolved a plethora of mechanisms to target host antiviral responses. Here, we propose a yet uncharacterized mechanism of immune regulation by the orthomyxovirus Thogoto virus (THOV) ML protein through engaging general transcription factor TFIIB. ML generates a TFIIB depleted nuclear environment by re-localizing it into the cytoplasm. Although a broad effect on gene expression would be anticipated, ML expression, delivery of an ML-derived functional domain or experimental depletion of TFIIB only leads to altered expression of a limited number of genes. Our data indicate that TFIIB is critically important for the </span><em>de novo</em><span>recruitment of Pol II to promoter start sites and that TFIIB may not be required for regulated gene expression from paused promoters. Since many immune genes require<span> </span></span><em>de novo</em><span>recruitment of Pol II, targeting of TFIIB by THOV represents a neat mechanism to affect immune responses while keeping other cellular transcriptional activities intact. Thus, interference with TFIIB activity may be a favourable site for therapeutic intervention to control undesirable inflammation.</span></p>',
'date' => '2018-04-30',
'pmid' => 'http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006980',
'doi' => '10.1371/journal.ppat.1006980',
'modified' => '2018-05-09 08:55:23',
'created' => '2018-05-09 08:55:23',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3369',
'name' => 'HMGB2 Loss upon Senescence Entry Disrupts Genomic Organization and Induces CTCF Clustering across Cell Types',
'authors' => 'Zirkel et. al.',
'description' => '<p><span>Processes like cellular senescence are characterized by complex events giving rise to heterogeneous cell populations. However, the early molecular events driving this cascade remain elusive. We hypothesized that senescence entry is triggered by an early disruption of the cells’ three-dimensional (3D) <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/genome-organization" title="Learn more about Genome Organization">genome organization</a>. To test this, we combined Hi-C, single-cell and population <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/transcriptome" title="Learn more about Transcriptome">transcriptomics</a>, imaging, and </span><em>in silico</em><span>modeling of three distinct cells types entering senescence. Genes involved in DNA conformation maintenance are suppressed upon senescence entry across all cell types. We show that nuclear depletion of the abundant<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/hmgb2" title="Learn more about HMGB2">HMGB2</a><span> </span>protein occurs early on the path to senescence and coincides with the dramatic spatial clustering of CT</span><em>CF.</em><span>Knocking down<span> </span></span><em>HMGB2</em><span><span> </span>suffices for senescence-induced<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/ctcf" title="Learn more about CTCF">CTCF</a><span> </span>clustering and for loop reshuffling, while ectopically expressing<span> </span></span><em>HMGB2</em><span><span> </span>rescues these effects. Our data suggest that HMGB2-mediated genomic reorganization constitutes a<span> </span><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/primer-molecular-biology" title="Learn more about Primer (molecular biology)">primer</a><span> </span>for the ensuing senescent program.</span></p>',
'date' => '2018-04-26',
'pmid' => 'https://www.sciencedirect.com/science/article/pii/S1097276518302338',
'doi' => '10.1016/j.molcel.2018.03.030',
'modified' => '2018-05-09 22:50:53',
'created' => '2018-05-09 22:50:53',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3337',
'name' => 'Widespread bacterial protein histidine phosphorylation revealed by mass spectrometry-based proteomics',
'authors' => 'Clement M Potel, Miao-Hsia Lin, Albert J R Heck, Simone Lemeer',
'description' => '<p>For decades, major difficulties in analyzing histidine phosphorylation have limited the study of phosphohistidine signaling. Here we report a method revealing widespread and abundant protein histidine phosphorylation in <i>Escherichia coli</i>. We generated an extensive <i>E. coli</i> phosphoproteome data set, in which a remarkably high percentage (<span class="stix"><span class="stix">∼</span></span>10%) of phosphorylation sites are phosphohistidine sites. This resource should help enable a better understanding of the biological function of histidine phosphorylation.</p>',
'date' => '2018-01-31',
'pmid' => 'https://www.nature.com/articles/nmeth.4580',
'doi' => '10.1038/nmeth.4580',
'modified' => '2018-02-11 02:37:05',
'created' => '2018-02-11 02:37:05',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 8 => array(
'id' => '3214',
'name' => 'MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1',
'authors' => 'Ann-Christin Hau, Britta Moyo Grebbin, Zsuzsa Agoston, Marie Anders-Maurer, Tamara Müller, Anja Groß, Jasmine Kolb, Julian D. Langer, Claudia Döring, Dorothea Schulte',
'description' => '<p><span>Pre–B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone–derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes: In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron-specific gene </span><em>doublecortin (Dcx)</em><span>. Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.</span></p>',
'date' => '2017-07-24',
'pmid' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'doi' => 'http://jcb.rupress.org/content/early/2017/07/24/jcb.201701154',
'modified' => '2017-08-29 11:17:03',
'created' => '2017-07-29 07:39:31',
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(int) 9 => array(
'id' => '3006',
'name' => 'reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells',
'authors' => 'Kinkley S et al.',
'description' => '<p>The combinatorial action of co-localizing chromatin modifications and regulators determines chromatin structure and function. However, identifying co-localizing chromatin features in a high-throughput manner remains a technical challenge. Here we describe a novel reChIP-seq approach and tailored bioinformatic analysis tool, normR that allows for the sequential enrichment and detection of co-localizing DNA-associated proteins in an unbiased and genome-wide manner. We illustrate the utility of the reChIP-seq method and normR by identifying H3K4me3 or H3K27me3 bivalently modified nucleosomes in primary human CD4(+) memory T cells. We unravel widespread bivalency at hypomethylated CpG-islands coinciding with inactive promoters of developmental regulators. reChIP-seq additionally uncovered heterogeneous bivalency in the population, which was undetectable by intersecting H3K4me3 and H3K27me3 ChIP-seq tracks. Finally, we provide evidence that bivalency is established and stabilized by an interplay between the genome and epigenome. Our reChIP-seq approach augments conventional ChIP-seq and is broadly applicable to unravel combinatorial modes of action.</p>',
'date' => '2016-08-17',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27530917',
'doi' => '',
'modified' => '2016-08-26 11:56:46',
'created' => '2016-08-26 11:38:15',
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(int) 10 => array(
'id' => '2981',
'name' => 'UMI-4C for quantitative and targeted chromosomal contact profiling',
'authors' => 'Omer Schwartzman, Zohar Mukamel, Noa Oded-Elkayam, Pedro Olivares-Chauvet, Yaniv Lubling, Gilad Landan, Shai Izraeli and Amos Tanay',
'description' => '<p>We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (umis) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.</p>',
'date' => '2016-07-04',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27376768',
'doi' => '10.1038/nmeth.3922',
'modified' => '2016-07-13 09:33:41',
'created' => '2016-07-13 09:32:29',
'ProductsPublication' => array(
[maximum depth reached]
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(int) 11 => array(
'id' => '2911',
'name' => 'Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock',
'authors' => 'Yoon Sik Kim, Young-Beom Kim, Woong Bin Kim, Seung Won Lee, Seog Bae Oh, Hee-Chul Han, C. Justin LeeEmail author, Christopher S. Colwell and Yang In Kim',
'description' => '<h3>Background</h3>
<p>Recent evidence indicates that histamine, acting on histamine 1 receptor (H1R), resets the circadian clock in the mouse suprachiasmatic nucleus (SCN) by increasing intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) through the activation of Ca<sub>V</sub>1.3 L-type Ca<sup>2+</sup> channels and Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release from ryanodine receptor-mediated internal stores.</p>
<h3>Results</h3>
<p>In the current study, we explored the underlying mechanisms with various techniques including Ca<sup>2+</sup>- and Cl<sup>−</sup>-imaging and extracellular single-unit recording. Our hypothesis was that histamine causes Cl<sup>−</sup> efflux through cystic fibrosis transmembrane conductance regulator (CFTR) to elicit membrane depolarization needed for the activation of Ca<sub>V</sub>1.3 Ca<sup>2+</sup> channels in SCN neurons. We found that histamine elicited Cl<sup>−</sup> efflux and increased [Ca<sup>2+</sup>]<sub>i</sub> in dissociated mouse SCN cells. Both of these events were suppressed by bumetanide [Na<sup>+</sup>-K<sup>+</sup>-2Cl<sup>−</sup> cotransporter isotype 1 (NKCC1) blocker], CFTR<sub>inh</sub>-172 (CFTR inhibitor), gallein (G<sub>βγ</sub> protein inhibitor) and H89 [protein kinase A (PKA) inhibitor]. By itself, H1R activation with 2-pyridylethylamine increased the level of cAMP in the SCN and this regulation was prevented by gallein. Finally, histamine-evoked phase shifts of the circadian neural activity rhythm in the mouse SCN slice were blocked by bumetanide, CFTR<sub>inh</sub>-172, gallein or H89 and were not observed in NKCC1 or CFTR KO mice.</p>
<h3>Conclusions</h3>
<p>Taken together, these results indicate that histamine recruits the H1R-G<sub>βγ</sub>-cAMP/PKA pathway in the SCN neurons to activate Ca<sub>V</sub>1.3 channels through CFTR-mediated Cl<sup>−</sup> efflux and ultimately to phase-shift the circadian clock. This pathway and NKCC1 may well be potential targets for agents designed to treat problems resulting from the disturbance of the circadian system.</p>',
'date' => '2016-03-22',
'pmid' => 'http://molecularbrain.biomedcentral.com/articles/10.1186/s13041-016-0227-1',
'doi' => '10.1186/s13041-016-0227-1',
'modified' => '2016-07-05 10:50:21',
'created' => '2016-05-11 07:38:13',
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(int) 12 => array(
'id' => '2810',
'name' => 'Standardizing chromatin research: a simple and universal method for ChIP-seq',
'authors' => 'Laura Arrigoni, Andreas S. Richter, Emily Betancourt, Kerstin Bruder, Sarah Diehl, Thomas Manke and Ulrike Bönisch',
'description' => '<p><span>Here we demonstrate that harmonization of ChIP-seq workflows across cell types and conditions is possible when obtaining chromatin from properly isolated nuclei. We established an ultrasound-based nuclei extraction method (Nuclei Extraction by Sonication) that is highly effective across various organisms, cell types and cell numbers. The described method has the potential to replace complex cell-type-specific, but largely ineffective, nuclei isolation protocols. This article demonstrates protocol standardization using the Bioruptor shearing systems and the IP-Star Automation System for ChIP automation.</span></p>',
'date' => '2015-12-23',
'pmid' => 'http://pubmed.gov/26704968',
'doi' => '10.1093/nar/gkv1495',
'modified' => '2016-06-09 09:47:00',
'created' => '2016-01-10 08:32:58',
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'id' => '2804',
'name' => 'HNF1β drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC)',
'authors' => 'Filipa Lopes-Coelho, Sofia Gouveia-Fernandes, Luís G Gonçalves, Carolina Nunes, Inês Faustino, Fernanda Silva, Ana Félix, Sofia A Pereira, Jacinta Serpa',
'description' => '<p>Chemoresistance to platinum-based antineoplastic agents is a consistent feature among ovarian carcinomas; however, whereas high-grade serous carcinoma (OSC) acquires resistance during chemotherapy, ovarian clear cell carcinoma (OCCC) is intrinsically resistant. The main objective of this study was to explore, in vitro and in vivo, if hepatocyte nuclear factor 1β (HNF1β) and glutaminolysis contribute for the resistance of OCCC to carboplatin through the intrinsically increased GSH bioavailability. To disclose the role of HNF1β, experiments were also performed in an OSC cell line, which does not express HNF1β. Metabolic profiles, GSH quantification, HNF1β, and γ-glutamylcysteine ligase catalytic subunit (GCLC) and modifier subunit (GCLM) expression, cell cycle, and death were assessed in ES2 cell line (OCCC) and OVCAR3 cell line (OSC); HNF1β knockdown was performed in ES2 and murine model of subcutaneous and peritoneal OCCC tumors was established to test buthionine sulphoxamine (BSO), as a sensitizer to carboplatin. Glutaminolysis is activated in ES2 and OVCAR3, though ES2 exclusively synthesizes amino acids and GSH. ES2 cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 to carboplatin. HNF1β regulates the expression of GCLC, but not GCLM, and consequently GSH production in ES2. In vivo, BSO prior to carboplatin reduces dramatically subcutaneous tumor size and GSH levels, as well as peritoneal dissemination. Our study discloses HNF1β as the mediator of intrinsic OCCC chemoresistance and sheds a light to re-explore a cancer adjuvant therapeutic approach using BSO to overcome the lack of efficient therapy in OCCC.</p>',
'date' => '2015-10-31',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26520442',
'doi' => '10.1007/s13277-015-4290-5',
'modified' => '2016-01-19 07:13:46',
'created' => '2015-12-13 09:42:34',
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'id' => '2795',
'name' => 'LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2',
'authors' => 'Maryem A. Hussein, Elina Shrestha, Mireille Ouimet, Tessa J. Barrett, Sarah Leone, Kathryn J. Moore, Yann Hérault, Edward A. Fisher, Michael J. Garabedian',
'description' => '<p>High cholesterol and diabetes are major risk factors for atherosclerosis. Regression of atherosclerosis is mediated in part by the Liver X Receptor (LXR) through the induction of genes involved in cholesterol transport and efflux. In the context of diabetes, regression of atherosclerosis is impaired. We proposed that changes in glucose levels modulate LXR-dependent gene expression. Using a mouse macrophage cell line (RAW 264.7) and primary bone marrow derived macrophages (BMDMs) cultured in normal or diabetes relevant high glucose conditions we found that high glucose inhibits the LXR-dependent expression of ATP-binding cassette transporter A1 (ABCA1), but not ABCG1. To probe for this mechanism, we surveyed the expression of a host of chromatin-modifying enzymes and found that Protein Arginine Methyltransferase 2 (PRMT2) was reduced in high compared to normal glucose conditions. Importantly, ABCA1 expression and ABCA1-mediated cholesterol efflux were reduced in Prmt2-/- compared to wild type BMDMs. Monocytes from diabetic mice also showed decreased expression of Prmt2 compared to non-diabetic counterparts. Thus, PRMT2 represents a glucose-sensitive factor that plays a role in LXR-mediated ABCA1-dependent cholesterol efflux and lends insight to the presence of increased atherosclerosis in diabetic patients.</p>',
'date' => '2015-08-19',
'pmid' => 'http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0135218',
'doi' => '10.1371/journal.pone.0135218',
'modified' => '2015-11-10 13:43:13',
'created' => '2015-10-27 10:16:07',
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(int) 15 => array(
'id' => '2647',
'name' => 'Sensitive detection of chromatin-altering polymorphisms reveals autoimmune disease mechanisms.',
'authors' => 'Del Rosario RC, Poschmann J, Rouam SL, Png E, Khor CC, Hibberd ML, Prabhakar S',
'description' => 'Most disease associations detected by genome-wide association studies (GWAS) lie outside coding genes, but very few have been mapped to causal regulatory variants. Here, we present a method for detecting regulatory quantitative trait loci (QTLs) that does not require genotyping or whole-genome sequencing. The method combines deep, long-read chromatin immunoprecipitation-sequencing (ChIP-seq) with a statistical test that simultaneously scores peak height correlation and allelic imbalance: the genotype-independent signal correlation and imbalance (G-SCI) test. We performed histone acetylation ChIP-seq on 57 human lymphoblastoid cell lines and used the resulting reads to call 500,066 single-nucleotide polymorphisms de novo within regulatory elements. The G-SCI test annotated 8,764 of these as histone acetylation QTLs (haQTLs)-an order of magnitude larger than the set of candidates detected by expression QTL analysis. Lymphoblastoid haQTLs were highly predictive of autoimmune disease mechanisms. Thus, our method facilitates large-scale regulatory variant detection in any moderately sized cohort for which functional profiling data can be generated, thereby simplifying identification of causal variants within GWAS loci.',
'date' => '2015-05-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25799442',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
'created' => '2015-07-24 15:39:05',
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(int) 16 => array(
'id' => '2643',
'name' => 'Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus, Osmeridae), an anadromous Pacific smelt.',
'authors' => 'Candy JR, Campbell NR, Grinnell MH, Beacham TD, Larson WA, Narum SR',
'description' => 'Twelve eulachon (Thaleichthys pacificus, Osmeridae) populations ranging from Cook Inlet, Alaska and along the west coast of North America to the Columbia River were examined by restriction-site-associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high geneflow species. A total of 4104 single-nucleotide polymorphisms (SNPs) were discovered across the genome, with 193 putatively adaptive SNPs as determined by FST outlier tests. Estimates of population structure in eulachon with the putatively adaptive SNPs were similar, but provided greater resolution of stocks compared with a putatively neutral panel of 3911 SNPs or previous estimates with 14 microsatellites. A cline of increasing measures of genetic diversity from south to north was found in the adaptive panel, but not in the neutral markers (SNPs or microsatellites). This may indicate divergent selective pressures in differing freshwater and marine environments between regional eulachon populations and that these adaptive diversity patterns not seen with neutral markers could be a consideration when determining genetic boundaries for conservation purposes. Estimates of effective population size (Ne ) were similar with the neutral SNP panel and microsatellites and may be utilized to monitor population status for eulachon where census sizes are difficult to obtain. Greater differentiation with the panel of putatively adaptive SNPs provided higher individual assignment accuracy compared to the neutral panel or microsatellites for stock identification purposes. This study presents the first SNPs that have been developed for eulachon, and analyses with these markers highlighted the importance of integrating genome-wide neutral and adaptive genetic variation for the applications of conservation and management.',
'date' => '2015-03-03',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25737187',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
'created' => '2015-07-24 15:39:05',
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(int) 17 => array(
'id' => '3076',
'name' => 'Silica nanoparticles induce oxidative stress, inflammation, and endothelial dysfunction in vitro via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling.',
'authors' => 'Caixia Guo,1,2 Yinye Xia,1,2 Piye Niu,1,2 Lizhen Jiang,1,2 Junchao Duan,1,2 Yang Yu,1,2 Xianqing Zhou,1,2 Yanbo Li,1,2 Zhiwei Sun1,2 ',
'description' => '<p><strong>Abstract:</strong><span> Despite the widespread application of silica nanoparticles (SiNPs) in industrial, commercial, and biomedical fields, their response to human cells has not been fully elucidated. Overall, little is known about the toxicological effects of SiNPs on the cardiovascular system. In this study, SiNPs with a 58 nm diameter were used to study their interaction with human umbilical vein endothelial cells (HUVECs). Dose- and time-dependent decrease in cell viability and damage on cell plasma-membrane integrity showed the cytotoxic potential of the SiNPs. SiNPs were found to induce oxidative stress, as evidenced by the significant elevation of reactive oxygen species generation and malondialdehyde production and downregulated activity in glutathione peroxidase. SiNPs also stimulated release of cytoprotective nitric oxide (NO) and upregulated inducible nitric oxide synthase (NOS) messenger ribonucleic acid, while downregulating endothelial NOS and ET-1 messenger ribonucleic acid, suggesting that SiNPs disturbed the NO/NOS system. SiNP-induced oxidative stress and NO/NOS imbalance resulted in endothelial dysfunction. SiNPs induced inflammation characterized by the upregulation of key inflammatory mediators, including IL-1β, IL-6, IL-8, TNFα, ICAM-1, VCAM-1, and MCP-1. In addition, SiNPs triggered the activation of the Nrf2-mediated antioxidant system, as evidenced by the induction of nuclear factor-κB and MAPK pathway activation. Our findings demonstrated that SiNPs could induce oxidative stress, inflammation, and NO/NOS system imbalance, and eventually lead to endothelial dysfunction via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling. This study indicated a potential deleterious effect of SiNPs on the vascular endothelium, which warrants more careful assessment of SiNPs before their application. </span></p>',
'date' => '2015-02-20',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25759575',
'doi' => '10.2147/IJN.S76114',
'modified' => '2016-11-29 10:33:26',
'created' => '2016-11-29 10:32:33',
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(int) 18 => array(
'id' => '2655',
'name' => 'Step-by-step preparation of proteins for mass spectrometric analysis.',
'authors' => 'Franz T, Li X.',
'description' => 'Nowadays, identification of proteins from biological samples by mass spectrometry is widely used. In principle there are two scenarios. Proteins are pre-fractionated in some way, e.g. by gel electrophoresis or are analyzed as complex mixture (shot gun). Shot gun proteomics became recently more popular, because of technological developments on the mass spectrometer side which allows now the identification of several thousand proteins from complex biological matrix. However, in many cases it is still useful to separate proteins first in a gel. But not only mass spectrometer technology made progress. This is also true for the sample preparation. Recently, protocols and techniques were developed which make the analysis of starting material in the low microgram range possible and also simplify the whole procedure. Detailed protocols will be described allowing also inexperienced beginners to get good results. ',
'date' => '0000-00-00',
'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/25820726',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
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'id' => '2600',
'name' => 'Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours',
'authors' => 'Bujko M, Kober P, Statkiewicz M, Mikula M, Ligaj M, Zwierzchowski L, Ostrowski J, Siedlecki JA',
'description' => 'Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). Results indicate that μ-protocadherin downregulation occurs during early stages of tumourigenesis and progression into the adenoma-carcinoma sequence. Epigenetic mechanisms are involved in this silencing.',
'date' => '0000-00-00',
'pmid' => 'http://www.hindawi.com/journals/grp/2015/317093/',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
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'description' => '<p>We use the <strong>Bioruptor</strong> sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The <strong>H3K4me3 antibody</strong> is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.</p>
<p>Diagenode provides not only very good products for research but also an excellent customer support.</p>',
'author' => 'Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany',
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'description' => '<p>To expedite the larger-scale phenotyping of pollen number difference within <em>Arabidopsis</em> family, we have modified an existing method more suitable for our purpose of pollen counting using a Bioruptor<sup>®</sup>. We grew four plants per genotype. Three flower buds per plant were harvested and dried at 65°C overnight. We collected flower buds with mature pollen, but before the anthers were opened (flower stage 13). We did not use the first and second flowers of the inflorescence, as they tend to show developmentally immature flower morphologies. 30 <span>μ</span>l of 5% Tween-20 is added to the dried flower and samples are sonicated using the Bioruptor<sup>®</sup> Plus with high power mode for 10 cycles (<span>sonication cycle: 30 sec ON, 30 sec OFF) </span>to separate pollen grains from the anthers. Then, all pollen solutions are measured with a cell counter.</p>',
'author' => 'Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland',
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$testimonials = '<blockquote><p>We use the <strong>Bioruptor</strong> sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The <strong>H3K4me3 antibody</strong> is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.</p>
<p>Diagenode provides not only very good products for research but also an excellent customer support.</p><cite>Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany</cite></blockquote>
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$featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>To expedite the larger-scale phenotyping of pollen number difference within <em>Arabidopsis</em> family, we have modified an existing method more suitable for our purpose of pollen counting using a Bioruptor<sup>®</sup>. We grew four plants per genotype. Three flower buds per plant were harvested and dried at 65°C overnight. We collected flower buds with mature pollen, but before the anthers were opened (flower stage 13). We did not use the first and second flowers of the inflorescence, as they tend to show developmentally immature flower morphologies. 30 <span>μ</span>l of 5% Tween-20 is added to the dried flower and samples are sonicated using the Bioruptor<sup>®</sup> Plus with high power mode for 10 cycles (<span>sonication cycle: 30 sec ON, 30 sec OFF) </span>to separate pollen grains from the anthers. Then, all pollen solutions are measured with a cell counter.</p><cite>Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland</cite></blockquote>
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'description' => '<p>To expedite the larger-scale phenotyping of pollen number difference within <em>Arabidopsis</em> family, we have modified an existing method more suitable for our purpose of pollen counting using a Bioruptor<sup>®</sup>. We grew four plants per genotype. Three flower buds per plant were harvested and dried at 65°C overnight. We collected flower buds with mature pollen, but before the anthers were opened (flower stage 13). We did not use the first and second flowers of the inflorescence, as they tend to show developmentally immature flower morphologies. 30 <span>μ</span>l of 5% Tween-20 is added to the dried flower and samples are sonicated using the Bioruptor<sup>®</sup> Plus with high power mode for 10 cycles (<span>sonication cycle: 30 sec ON, 30 sec OFF) </span>to separate pollen grains from the anthers. Then, all pollen solutions are measured with a cell counter.</p>',
'author' => 'Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland',
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<a href="/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack"><img src="/img/product/shearing_technologies/B01200040_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200011</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1792" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1792" id="CartAdd/1792Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1792" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 1.5 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('1.5 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200011',
'1850',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('1.5 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200011',
'1850',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack" data-reveal-id="cartModal-1792" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">1.5 ml tube holder for Bioruptor® Standard ...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack"><img src="/img/product/shearing_technologies/B01200013_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200013</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1794" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1794" id="CartAdd/1794Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1794" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'B01200013',
'1850',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'B01200013',
'1850',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack" data-reveal-id="cartModal-1794" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml tube holder for Bioruptor® Standard &...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack"><img src="/img/product/shearing_technologies/B01200014_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200014</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1795" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1795" id="CartAdd/1795Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1795" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'B01200014',
'1850',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('50 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus',
'B01200014',
'1850',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack" data-reveal-id="cartModal-1795" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">50 ml tube holder for Bioruptor® Standard &...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/water-cooler-115v-1-unit"><img src="/img/product/shearing_technologies/B02010003%20_water_cooler.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02010003</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1814" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1814" id="CartAdd/1814Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1814" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Water cooler, 115V</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 115V',
'B02010003',
'4950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 115V',
'B02010003',
'4950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="water-cooler-115v-1-unit" data-reveal-id="cartModal-1814" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Water cooler, 115V</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/water-cooler-230v-1-unit"><img src="/img/product/shearing_technologies/B02010003%20_water_cooler.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02010002</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1813" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1813" id="CartAdd/1813Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1813" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Water cooler, 230V</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 230V',
'B02010002',
'4950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Water cooler, 230V',
'B02010002',
'4950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="water-cooler-230v-1-unit" data-reveal-id="cartModal-1813" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Water cooler, 230V</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/single-cycle-valve-for-water-cooler-1-unit"><img src="/img/product/shearing_technologies/B02020004%20_single_cycle_valve.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B02020005</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1816" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1816" id="CartAdd/1816Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1816" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Single Cycle Valve for Water Cooler</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Single Cycle Valve for Water Cooler',
'B02020005',
'950',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="single-cycle-valve-for-water-cooler-1-unit" data-reveal-id="cartModal-1816" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Single Cycle Valve for Water Cooler</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/metallic-soundproof-box-1-unit"><img src="/img/product/shearing_technologies/B01200001%20_soundproof_box.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200001</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1790" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1790" id="CartAdd/1790Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1790" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Metallic soundproof box</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Metallic soundproof box',
'B01200001',
'1450',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="metallic-soundproof-box-1-unit" data-reveal-id="cartModal-1790" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Metallic soundproof box</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-ml-bioruptor-microtubes-500-tubes"><img src="/img/product/shearing_technologies/C30010013_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010013</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2627" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2627" id="CartAdd/2627Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2627" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5 ml Bioruptor<sup>®</sup> Plus Microtubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5 ml Bioruptor<sup>®</sup> Plus Microtubes',
'C30010013',
'145',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-ml-bioruptor-microtubes-500-tubes" data-reveal-id="cartModal-2627" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5 ml Bioruptor® Plus Microtubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200043_tube_holder.jpg" alt="Product image" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200043</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1809" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1809" id="CartAdd/1809Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1809" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('0.5/0.65 ml tube holder for Bioruptor<sup>®</sup> Standard & Plus',
'B01200043',
'1800',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1809" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">0.5/0.65 ml tube holder for Bioruptor® Stan...</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010009</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Plus TPX tubes</strong> to my shopping cart.</p>
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<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
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<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
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<li>
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<a href="/en/p/rna-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
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<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> RNA Extraction kit for Bioruptor Plus</strong> to my shopping cart.</p>
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<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
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<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('RNA Extraction kit for Bioruptor Plus',
'C20000010',
'165',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="rna-extraction-kit-50-rxns" data-reveal-id="cartModal-2616" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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<h6 style="height:60px">RNA Extraction kit for Bioruptor Plus</h6>
</div>
</div>
</li>
<li>
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction kit for Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
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</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-kit-50-rxns" data-reveal-id="cartModal-2617" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
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</li>
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction beads</strong> to my shopping cart.</p>
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<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads',
'C20000021',
'100',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
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<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads',
'C20000021',
'100',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
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</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-beads-20-g" data-reveal-id="cartModal-2618" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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</li>
<li>
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Bioruptor<sup>®</sup> Plus DNA QC kit</strong> to my shopping cart.</p>
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<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Bioruptor<sup>®</sup> Plus DNA QC kit',
'C40010001',
'135',
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<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Bioruptor<sup>®</sup> Plus DNA QC kit',
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</div>
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</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="Bioruptor-Plus-DNA-QC-kit" data-reveal-id="cartModal-2835" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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</div>
<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus DNA QC kit</h6>
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</div>
</li>
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'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®',
'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>',
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'modified' => '2016-04-29 17:39:29',
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'modified' => '2023-08-31 14:27:41',
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'description' => 'Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in three key types of colorectal tissue samples, for normal mucosa, adenoma, and carcinoma. A gradual decrease of mRNA levels and protein expression was observed in progressive stages of colorectal carcinogenesis which are consistent with reports of increasing MUPCDH 5′ promoter region DNA methylation. High MUPCDH methylation was also observed in HCT116 and SW480 CRC cell lines that revealed low gene expression levels compared to COLO205 and HT29 cell lines which lack DNA methylation at the MUPCDH locus. Furthermore, HCT116 and SW480 showed lower levels of RNA polymerase II and histone H3 lysine 4 trimethylation (H3K4me3) as well as higher levels of H3K27 trimethylation at the MUPCDH promoter. MUPCDH expression was however restored in HCT116 and SW480 cells in the presence of 5-Aza-2′-deoxycytidine (DNA methyltransferase inhibitor). Results indicate that μ-protocadherin downregulation occurs during early stages of tumourigenesis and progression into the adenoma-carcinoma sequence. Epigenetic mechanisms are involved in this silencing.',
'date' => '0000-00-00',
'pmid' => 'http://www.hindawi.com/journals/grp/2015/317093/',
'doi' => '',
'modified' => '2015-07-24 15:39:05',
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$externalLink = ' <a href="http://www.hindawi.com/journals/grp/2015/317093/" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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