Unparalleled ChIP-Seq results with the most rigorously validated antibodies
Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.
ChIP-seq profile of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.
ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region.
Diagenode’s highly validated antibodies:
| Cat. No. | Product | Application | Purity | |
|---|---|---|---|---|
| C15410367 |
PPARg Antibody (50 µg) |
ChIP-qPCR ChIP-seq ELISA | Affinity purified polyclonal antibody. | |
| C15310266 |
AF9 Antibody (100 μl) |
ChIP-qPCR ChIP-seq | Whole antiserum from rabbit containing 0.05% azide. | |
| C15310197 |
AML1-ETO Antibody (100 µl) |
ChIP-qPCR ChIP-seq ELISA WB | Whole antiserum | |
| C15410337 |
BRD4 Antibody (50 µg) |
ChIP-seq ELISA WB | Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300. | |
| C15310002 |
CBFb Antibody (100 µl) |
ChIP-qPCR ChIP-seq ELISA | Whole antiserum | |
| C15410339 |
CBX2 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IP WB | Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. | |
| C15410333 |
CBX8 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IP WB | Affinity purified polyclonal antibody. | |
| C15410334 |
CHD1 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IP WB | Affinity purified | |
| C15410340 |
CHD7 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IP WB | Affinity purified polyclonal antibody in TBS containing 0.1% BSA and 0.09% azide. | |
| C15410210 |
CTCF Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA IF siRNA Knockdown WB | Affinity purified | |
| C15410363 |
EHF Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA | Affinity purified polyclonal antibody. | |
| C15100066-100 |
ERalpha Antibody (100 µl) |
ChIP-qPCR ChIP-seq ELISA GSA IC IP WB | Ascites fluid | |
| C15310001 |
ETO Antibody (100 µl) |
ChIP-qPCR ChIP-seq ELISA | Whole antiserum | |
| C15410039 |
EZH2 Antibody (50 µg) |
ChIP-qPCR ChIP-seq IF siRNA Knockdown WB | Protein G purified | |
| C15410231-100 |
FOXA1 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IF IHC IP WB | Affinity purified | |
| C15410343 |
FOXA2 Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA WB | Affinity purified polyclonal antibody in PBS containing 0.05% azide. | |
| C15410232-100 |
FOXM1 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IHC WB | Affinity purified | |
| C15200010 |
GR Antibody (50 µg) |
ChIP-qPCR ChIP-seq ELISA Flow Cyt GSA IP WB | Ammonium sulphate purified | |
| C15410201 |
H2A.Z Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA IF WB | Affinity purified | |
| C15210021 |
H2A.Z Antibody (100 µg) |
ChIP-seq IF WB | Affinity purified polyclonal antibody | |
| C15410173 |
H2A.Zac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF | Affinity purified | |
| C15410202 |
H2A.Zac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15210009 |
H2A.ZK4ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
| C15210012 |
H2A.ZK7ac Antibody (100 μg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
| C15410215 |
H2AK5ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15410212 |
H2BK12ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15410220 |
H2BK15ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15210010 |
H2BK20ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
| C15210011 |
H3.3 Antibody (100 µg) |
ChIP-qPCR ChIP-seq WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
| C15210005 |
H3K14ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Protein A purified monoclonal antibody in PBS containing 1% BSA and 0.09% azide. | |
| C15410139 |
H3K18ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
| C15210020 |
H3K18ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Affinity purified monoclonal antibody | |
| C15410344 |
H3K23ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody in PBS containing 0.05% azide. | |
| C15210007 |
H3K23me2 Antibody (100 µg) |
ChIP-qPCR ChIP-seq WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
| C15410196 |
H3K27ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
| C15410174 |
H3K27ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified polyclonal antibody. | |
| C15210016 |
H3K27ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Affinity purified polyclonal antibody. | |
| C15410069 |
H3K27me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified polyclonal antibody. | |
| C15410195 |
H3K27me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
| C15210017 |
H3K27me3 Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB WB | Affinity purified polyclonal antibody. | |
| C15410091 |
H3K27me3S28p Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
| C15410307 |
H3K36ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
| C15310127 |
H3K36me2 polyclonal antibody (100 µl) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Whole antiserum from rabbit. | |
| C15310058 |
H3K36me3 Antibody (100 µl) |
ChIP-qPCR ChIP-seq DB ELISA WB | Whole antiserum from rabbit containing 0.05% azide. | |
| C15410058 |
H3K36me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
| C15410192 |
H3K36me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified polyclonal antibody. | |
| C15210013 |
H3K36me3 Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB WB | Affinity purified polyclonal antibody. | |
| C15410322 |
H3K4ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody | |
| C15410037 |
H3K4me1 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody. | |
| C15410194 |
H3K4me1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
| C15210018 |
H3K4me1 Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Affinity purified polyclonal antibody. | |
| C15410035 |
H3K4me2 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified polyclonal antibody. | |
| C15200152 |
H3K4me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA IF WB | Protein A purified monoclonal antibody. | |
| C15410003 |
H3K4me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified polyclonal antibody. | |
| C15410213 |
H3K56ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA WB | Affinity purified | |
| C15210006 |
H3K79ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified | |
| C15410082 |
H3K79me1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA WB | Affinity purified | |
| C15410051 |
H3K79me2 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15410068 |
H3K79me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody | |
| C15410005 |
H3K9/14ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15410200 |
H3K9/14ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
| C15410004 |
H3K9ac Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15410177 |
H3K9ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
| C15210015 |
H3K9ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB WB | Affinity purified polyclonal antibody. | |
| C15410056 |
H3K9me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody. | |
| C15410193 |
H3K9me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified polyclonal antibody. | |
| C15500003 |
H3K9me3 recombinant antibody and negative control (50 µg) |
ChIP-qPCR ChIP-seq IF | affinity purified | |
| C15410171 |
H3R17me2(asym)K18ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15410331 |
H4K12ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
| C15210008 |
H4K20ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
| C15200147 |
H4K20me1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB IF WB | Protein A purified | |
| C15410207 |
H4K20me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
| C15410057 |
H4K20me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
| C15410024 |
H4K5,8,12,16ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB IF | Affinity purified polyclonal antibody. | |
| C15410021 |
H4K5,8,12ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
| C15410025 |
H4K5ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
| C15410325 |
HDAC1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA IF Protein Analysis siRNA Knockdown WB | Affinity purified | |
| C15410053 |
HDAC1 Antibody - replaced by the reference C15410325 (50 µg) |
ChIP-qPCR ChIP-seq ELISA IF WB | Affinity purified | |
| C15200201 |
HDAC2 Antibody (50 μg) |
ChIP-qPCR ChIP-seq IF siRNA Knockdown WB | Protein A purified | |
| C15410364 |
HDAC3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq ELISA | Affinity purified polyclonal antibody. | |
| C15410362 |
HDAC6 Antibody (50 µg) |
ChIP-qPCR ChIP-seq ELISA | Affinity purified polyclonal antibody. | |
| C15410338 |
JARID1C Antibody (100 μl) |
ChIP-qPCR ChIP-seq IP WB | Affinity purified polyclonal antibody in TBS containing 0.1% BSA and 0.09% azide. | |
| C15410332 |
JMJD2a Antibody (100 μl) |
ChIP-qPCR ChIP-seq IP WB | Affinity purified | |
| C15410335 |
KAT2B Antibody (100 μl) |
ChIP-qPCR ChIP-seq WB | Affinity purified | |
| C15410067 |
LSD1 Antibody (50 µg) |
ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB | Affinity purified | |
| C15210003 |
macroH2A.1/H2A.2 Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified | |
| C15310264 |
MLL1 Antibody (100 µl) |
ChIP-qPCR ChIP-seq ELISA | Whole antiserum from rabbit containing 0.05% azide. | |
| C15310254 |
MYH11 Antibody (100 μl) |
ChIP-qPCR ChIP-seq ELISA WB | Whole antiserum | |
| C15410341 |
NCOR1 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IP WB | Affinity purified polyclonal antibody. | |
| C15410240-100 |
NF-E2 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IF WB | Affinity purified | |
| C15310256 |
NFKB p65 Antibody (100 μl) |
ChIP-qPCR ChIP-seq ELISA IHC WB | Whole antiserum | |
| C15310261 |
NFYA Antibody (100 μl) |
ChIP-qPCR ChIP-seq IHC WB | Whole antiserum | |
| C15410241-100 |
NFYB Antibody (100 μl) |
ChIP-qPCR ChIP-seq IF IP WB | Affinity purified | |
| C15200013 |
NRF1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq WB | Protein A purified | |
| C15410305 |
OCT4 Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA WB | Affinity purified | |
| C15200211 |
p300 Antibody (50 μg) |
ChIP-qPCR ChIP-seq | Protein A purified monoclonal antibody | |
| C15410083 |
p53 Antibody (50 µg / 28 µl) |
ChIP-qPCR ChIP-seq ELISA WB | Affinity purified | |
| C15200004 |
Pol II Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB | Protein A purified monoclonal antibody. | |
| C15200253 |
Pol II Antibody (50 µg) |
Protein A purified monoclonal antibody | ||
| C15100055-100 |
Pol II Antibody - replaced by the antibody C15200253 (100 µl) |
ChIP-qPCR ChIP-seq siRNA Knockdown WB | Ascites fluid | |
| C15200005 |
Pol II S2p Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB | Affinity purified monoclonal antibody in PBS containing 0.05% azide. | |
| C15200007 |
Pol II S5p Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB | Affinity purified monoclonal antibody in PBS containing 0.05% azide. | |
| C15310155 |
RARA Antibody (100 µl) |
ChIP-qPCR ChIP-seq ELISA WB | Whole antiserum | |
| C15410342 |
RBBP5 Antibody (100 μl) |
ChIP-qPCR ChIP-seq IHC IP WB | Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. | |
| C15410036 |
SAP30 Antibody (50 µg) |
ChIP-qPCR ChIP-seq WB | Protein G purified | |
| C15410274 |
SMAD1 Antibody (100 μg) |
ChIP-qPCR ChIP-seq WB | Affinity purified | |
| C15410368 |
SUV39H1 Antibody (50 µg) |
ChIP-seq ELISA WB | Affinity purified polyclonal antibody | |
| C15410266-100 |
TARDBP Antibody (100 μl) |
ChIP-qPCR ChIP-seq IF IHC IP WB | Affinity purified | |
| C15200002 |
TBP Antibody (100 µg) |
ChIP-qPCR ChIP-seq siRNA Knockdown WB | Ammonium sulphate purified | |
| C15410345 |
YY1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq ELISA WB | Affinity purified polyclonal antibody in PBS containing 0.05% azide. | |
| C15200016 |
ZMYM3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq Protein array | Protein A purified monoclonal antibody in PBS containing 0.05% azide. |
